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All Journal Jurnal Review Pendidikan dan Pengajaran (JRPP) MAHESA : Malahayati Health Student Journal Innovative: Journal Of Social Science Research Jurnal Laboratorium Khatulistiwa Jurnal Riset Ilmiah Indonesian Journal of Community Empowerment Media Penelitian dan Pengembangan Kesehatan MEDICA (International Medical Scientific Journal) Aksi Kita: Jurnal Pengabdian Kepada Masyarakat Jurnal Info Kesehatan ELECTRON (Journal of Science and Technology)
Imma Fatayati
Poltekkes Kemenkes Pontianak
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Identifikasi Bakteri Gram Negatif Pada Air Tebu Di Wilayah Kota Pontianak Destia Aufani; Supriyanto Supriyanto; Imma Fatayati; Bagus Muhammad Ihsan
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v3i6.6781

Abstract

Air tebu merupakan minuman yang terbuat dari perasan batang tebu yang diperas dengan menggunakan mesin khusus membuatnya memiliki rasa yang manis alami, minuman ini sangat digemari oleh masyarakat. Akan tetapi kontaminasi mikroba pada air tebu sangat tinggi sehingga dapat menyebabkan berbagai penyakit. Berbagai bakteri dari genus Escherichia, Salmonella, Klebsiella, Shigella, dan Enterobacter yang merupakan salah satu indikator polusi limbah dan kondisi yang tidak menguntungkan untuk makanan dan minuman. Penelitian ini bertujuan untuk mengetahui bakteri yang terdapat pada minuman air tebu di Kota Pontianak. Penelitian ini menggunakan beberapa sampel air tebu di wilayah kota Pontianak dengan menggunakan metode kultur. Hasil yang didapatkan dari 70 sampel yaitu mendapatkan 8 sampel positif bakteri Enterobacter aerogenes, 4 sampel positif bakteri Salmonella typhi, 14 sampel positif Klebsiella pneumonia, 6 sampel positif Shigella dysentriae, dan 15 sampel positif Escherichia coli.
EFEKTIFITAS KOMBUCHA TEH BUNGA ROSELLA DALAM MENGHAMBAT PERTUMBUHAN BAKTERI ESHERICHIA COLI DAN SHIGELLA DYSENTERIAE UNTUK MENCEGAH TERJADINYA DIARE Fatayati, Imma; Djohan, Herlinda; Roofiif, Sayyid Al
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1348

Abstract

One probiotic product is kombucha tea. Kombucha tea can be combined with rosella tea. Some of the bacteria that attack the digestive tract are Escherichia Coli and Shigella dysenteriae which can cause diarrhea. It has been suggested that probiotics can be used to prevent diarrhea. This research aims to determine whether there are probiotic benefits of Rosella flower kombucha fermented for 7 days and 14 days to prevent diarrhea. Review of the growth inhibitory power of Escherichia Coli and Shigella dysenteriae bacteria. This research is in the form of Quasi-experimental. The population of this study was rosella flower kombucha. The sample for this research was rosella flower kombucha with 7 days of fermentation and 14 days of fermentation. The number of repetitions for each treatment was 16 replications, with total replications was 62 replications. The results of this research showed that the inhibitory power of rosella flower kombucha against Shigella dysenteriae bacteria with an average fermentation time of 7 days was 11.31 mm (strong), for 14 days of fermentation the average was 24.72 mm (very strong). Meanwhile, the inhibitory power of rosella flower kombucha against E. Coli bacteria with a fermentation time of 7 days averaged 11.09 mm (strong), for 14-day fermentation, the average was 20.34 mm (very strong). This research concluded that the inhibitory power of rosella flower kombucha fermented for 7 days was in the strong category and 14 days in the very strong category for both the growth of Shigella dysentriae and `E. coli bacteria.
ANALISIS ANTI BAKTERI SEDIAAN GEL PEMBERSIH TANGAN EKSTRAK ETANOL DAUN BELIMBING WULUH TERHADAP BAKTERI Escherichia coli Sya’baniar, Luthfiranda; Suwandi, Edy; Ihsan, Bagus Muhammad; Sari, Emilda; Fatayati, Imma
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1492

Abstract

Antiseptik digunakan untuk membunuh atau menghambat pertumbuhan mikroorganisme pada jaringan hidup. Daun belimbing wuluh mengandung senyawa metabolit antibakteri seperti alkaloid, flavonoid, saponin, tanin, fenol, steroid dan triterpenoid. Tujuan penelitian ini menjelaskan perbedaan gel pembersih tangan ekstrak daun belimbing wuluh dua macam formula dalam menghambat Escherichia coli. Dengan purposive sampling, sampel yang digunakan adalah ekstrak daun belimbing wuluh dengan 2 perlakuan yaitu kosentrasi 7,5% dan 15% yang masing-masing dilakukan pengulangan sebanyak 16 kali dengan metode difusi cakram. Hasil penelitian yaitu sediaan gel pembersih tangan memiliki aktivitas antibakteri terhadap Escherichia coli pada formula I memiliki zona hambat rata-rata 7,4mm dan formula II memiliki zona hambat rata-rata 9,6mm kategori sedang. Hasil organoleptis warna hijau bau khas berbentuk semi solid dan rata-rata pH 6. Hasil uji Wilcoxon didapatkan p value 0,000 < α 0,05 artinya terdapat perbedaan gel pembersih tangan ekstrak daun belimbing wuluh formula I dan formula II dalam mengahambat pertumbuhan Escherichia coli.
KORELASI ANTARA RASIO NEUTROFIL/LIMFOSIT DENGAN KADAR C-REACTIVE PROTEIN PADA PENDERITA TUBERKULOSIS PARU Aufani, Destia; Azzahra, Larasabella; Supriyanto, Supriyanto; Fatayati, Imma; Nuswantoro, Ari
Media Penelitian dan Pengembangan Kesehatan Vol. 33 No. 2 (2023): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v33i2.2089

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis and can persist in all parts of the body. Laboratory tests used to determine the level of inflammation in TB sufferers include the neutrophil/lymphocyte ratio (RNL) and C-reactive protein (CRP) levels. The RNL value is the ratio of the absolute number of neutrophils and the absolute number of lymphocytes, while CRP is an acute phase protein produced in the liver, and its levels increase within 6 hours in acute inflammation. The aim of this study was to determine the relationship between RNL and CRP in pulmonary TB patients. This research was a descriptive study with a cross-sectional design carried out from April to June 2021 at the Lung Health Services Integrated Service Unit, West Kalimantan Province. The number of samples was determined using a purposive sampling technique, with the criteria being newly diagnosed TB patients who had positive BTA, positive TCM and positive X-ray results, resulting in 48 samples. CRP levels were measured using the latex agglutination method, while the number of neutrophils and lymphocytes used the impedance method. Analysis was carried out using the Shapiro-Wilk normality test and Kendall's tau b correlation test. Statistical analysis using the Kendall's tau b correlation test obtained a significant value of 0.000 (<0.05), which means there was a relationship between RNL and CRP levels in pulmonary TB patients with a correlation coefficient of 0.489, which means the relationship between the two variables is enough.
Evaluasi Robustness Metode Deteksi Cepat Berbasis MPN untuk Legionella pneumophila terhadap Gangguan Mikroflora Non-Target pada Sampel Air dari Menara Pendingin Ulfia, Phisa Isyah; Sungkawa, Hendra Budi; Fatayati, Imma
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1887

Abstract

Legionellosis is an infection caused by Legionella pneumophila, commonly transmitted through artificial water systems such as cooling towers. Early detection of this pathogen is crucial, particularly in high-risk facilities. This study aimed to assess the robustness of a rapid detection method based on the Most Probable Number (MPN) principle, focusing on the effectiveness of pretreatment in reducing interference from non-target microorganisms. A total of 30 positive trays from Legiolert™ tests were subcultured on Tryptic Soy Agar (TSA) to identify any surviving non-target microbes. Robustness was defined by the proportion of trays without non-target microbial growth. Results showed a robustness rate of 93.33%, with full robustness (100%) observed in low and moderate microbial load groups, and 75% in the high-load group. A non-parametric binomial test using SPSS was performed to evaluate the statistical significance of the deviation from the robustness cut-off. The resulting p-value was 0.446 (p > 0.05), indicating no statistically significant difference. These findings demonstrate that the Legiolert™ method maintains reliable performance even in complex sample matrices, supporting its use in routine water quality monitoring in accordance with Indonesian Ministry of Health Regulation No. 2 of 2023.
ANALISIS SEDIAAN FOOT SPRAY EKSTRAK ETANOL DAUN BELIMBING WULUH TERHADAP BAKTERI Staphylococcus aureus Fatayati, Imma; Farwa, Syalsabilla; Tumpuk, Sri; Wahdaniah, Wahdaniah
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1821

Abstract

The appearance of foot odor is due to the emergence of sweat in the apocrine glands which contain organic ingredients such as the amino acid leucine. Bacteria on the feet have the degrading enzyme leucine dehydrogenase which will degrade the amino acid leucine in sweat into isovaleric acid which is an odorous compound. Foot spray is a foot odor removal product that dries faster and is easier to use. Starfruit leaves contain alkaloids, flavonoids, saponins, tannins and terpenoids as antibacterials. This research aims to explain the size of the inhibition zone formed in five formulas to inhibit Staphylococcus aureus bacteria. This research uses a Quasi Experiment design. The sample used was foot spray of starfruit leaf extract with five treatments, namely formula I (concentration 10%), formula II (concentration 20%), formula III (concentration 30%), formula IV (concentration 40%) and formula V (concentration 50%) with 5 replications so that the number of samples used was 25 samples which were tested for strength against Staphylococcus aureus using the diffusion method. Based on the results of the inhibition test, the inhibition zone formed in formula I was an average of 9.2 mm (moderat), formula II was 10.6 mm (moderat), formula III was 11.4 mm (stronge), formula IV was 12 mm (stronge) and formula V was 12.6 mm (stronge). From the results Friedman test, it was found that p value = 0.002 < α 0.05 so that there were differences in foot spray preparations of ethanol extract of starfruit leaves formulas I, II, III, IV and V against Staphylococcus aureus.
Evaluation of the accuracy and precision of the microlab 300 device for total cholesterol test on the CHOD-PAP method Ananda, Fanny Rizki; Sungkawa, Hendra Budi; Kamilla, Laila; Fatayati, Imma
ELECTRON (Journal of Science and Technology) Vol. 7 No. 1 (2025): ELECTRON (Journal of Science and Technology)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/electron.v7i1.516

Abstract

Internal quality assurance (PMI) is a routine preventive and monitoring activity in the laboratory to minimize errors and produce accurate tests. Quality accuracy and precision are important indicators in assessing PMI. Accuracy indicates the closeness of the test results to the true value, while precision describes the closeness of the results of repeated tests on the same sample. Total cholesterol examination using a spectrophotometer, especially the CHOD-PAP method, is often carried out at the Clinical Laboratory of the Poltekkes Kemenkes Pontianak due to its stability. This observational study aims to evaluate the accuracy and precision of the Microlab 300 tool for total cholesterol examination using the CHOD-PAP method at the Integrated Laboratory of the Poltekkes Kemenkes Pontianak. The study population was the assayed control serum solution, with samples in the form of normal control serum solution dialab which was divided into 100 vials containing 50 µL. A total of 30 vials were used for the preliminary period and 30 vials for the control period. The parameter examined was total cholesterol by CHOD-PAP method. The results showed the average accuracy of the Microlab 300 tool was 96.08% and precision was 99.21%. The daily graph using Westgard's rule shows that the control material is within the control limits. Thus, it can be concluded that the Microlab 300 tool shows good accuracy and precision in the examination of total cholesterol CHOD-PAP method in the laboratory
The Relationship Between Cholesterol Levels and the Incidence of Preeclampsia in Pregnant Women at Gang Sehat Community Health Center Nurisma, Yuni; Fatayati, Imma; Ihsan, Bagus Muhammad; Mikrani, Ashfaque Raza
MEDICA (International Medical Scientific Journal) Vol. 7 No. 2 (2025): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i2.510

Abstract

Preeclampsia, a dangerous pregnancy complication characterized by elevated blood pressure (>140/90 mmHg), proteinuria, and edema, is a leading cause of maternal mortality. Various risk factors have been identified, including a history of hypertension, age, body mass index (BMI), parity, stress, knowledge level, dietary patterns, exposure to cigarette smoke, and lipid metabolism changes—particularly elevated cholesterol levels. This study aims to analyze the relationship between cholesterol levels and the incidence of preeclampsia among pregnant women at Gang Sehat Community Health Center, Pontianak City. Using a descriptive-analytic design with a cross-sectional approach and purposive sampling technique, the study involved 48 pregnant women with gestational age over 20 weeks during the period of May to June 2024. The results showed that 23 respondents (47.9%) had high cholesterol levels. Interestingly, all 17 respondents (35.4%) who experienced preeclampsia had high cholesterol levels. Meanwhile, among the pregnant women who did not develop preeclampsia, 6 respondents (19.4%) had high cholesterol levels, while 25 respondents (80.6%) had normal cholesterol levels. Statistical analysis using the Chi-Square test revealed a significant correlation between cholesterol levels and the incidence of preeclampsia in pregnant women, with an Asymptotic Significance value of < 0.001 (p < 0.05). In conclusion, there is a significant relationship between cholesterol levels and the incidence of preeclampsia in pregnant women at Gang Sehat Community Health Center, Pontianak City.
The potential of Cilembu sweet potato (Ipomoea batatas L.) as a growth medium for Staphylococcus aureus and Escherichia coli Nuswantoro, Ari; Rahayu, Merda Sri; Fatayati, Imma; Syopingi, Syopingi; Maretalinia, Maretalinia
JURNAL INFO KESEHATAN Vol 22 No 1 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss1.1087

Abstract

The media aims to store, reproduce, and identify bacteria but has disadvantages such as being expensive, containing chemicals, and being easily damaged due to contamination. Therefore we need an alternative media that can overcome these limitations. Cilembu sweet potato (Ipomoea batatas, L.) is a natural ingredient that is easy to obtain and contains sufficient nutrients so it has the potential to be used as a basic ingredient for growth media. The purpose of this study was to determine the potential of Cilembu sweet potato flour as an alternative medium for the growth of Staphylococcus aureus and Escherichia coli bacteria with a quasi-experimental design. Cilembu sweet potato flour is obtained by cleaning, chopping, drying in the oven, crushing with a blender, and sifting the tubers to obtain fairly fine flour. The flour was dissolved in agar and then inoculated with S. aureus and E. coli, each with 16 replications. The results showed that the average number of S. aureus colonies was 119.12 CFU (169.2 CFU in control) while E. coli was 160.56 CFU (221.2 CFU in control). The Mann-Whitney test showed that there was a difference in the number of S. aureus colonies on alternative media and NA (p = 0.006 ≤ 0.05), but there was no difference between the number of E. coli colonies on alternative media and NA (p = 0.057 > 0.05). Finally, there was a difference in the number of S. aureus and E. coli colonies on alternative media (p = 0.04 ≤ 0.05). The nutritional composition shows that Cilembu sweet potato flour has more potential to replace NA as a growth medium for E. coli than for S. aureus.
Identifikasi Bakteri Klebsiella pneumoniae pada SWAB Luka Diabetes Mellitus di Klinik Perawatan Luka Kota Pontianak Labunda, Aura Iqlima; Fatayati, Imma; Slamet, Slamet
MAHESA : Malahayati Health Student Journal Vol 4, No 2 (2024): Volume 4 Nomor 2 (2024)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v4i2.13207

Abstract

ABSTRACT Diabetes mellitus (DM) is a metabolic disease that occurs due to hyperglycemia. DM wounds are caused by the onset of bacterial infection in the wound. Wounds in DM patients can be at risk of infection and may result in amputation. Klebsiella pneumoniae bacteria can cause pneumonia, infect wounds, and even bloodstream infections. Identifying Klebsiella pneumoniae bacteria present in DM wounds. Observational description with a cross-sectional design, which identified the species of Klebsiella pneumoniae in DM wounds by culture. The discovery of Klebsiella pneumoniae bacteria in DM wounds. Of the 28 DM wound swab samples conducted at the diabetes treatment clinic, Pontianak City, 20 samples were positive for Klebsiella pneumoniae (71.42%) and 8 samples were negative for Klebsiella pneumoniae (28.57%). Keywords: Gram-Negative Bacteria, Diabetes Mellitus, Wound  ABSTRAK Diabetes mellitus (DM) adalah penyakit metabolik yang terjadi karena hiperglikemia. Luka DM disebabkan oleh timbulnya infeksi bakteri pada luka. Luka pada pasien DM dapat berisiko mengalami infeksi dan dapat berakhir amputasi. Bakteri Klebsiella pneumoniae dapat menyebabkan penyakit pneumonia, menginfeksi luka, bahkan sampai infeksi aliran darah.    Mengidentifikasi bakteri Klebsiella pneumoniae yang ada pada luka DM. Deskripsi observasi dengan desain cross-sectional, yaitu mengidentifikasi spesies dari Klebsiella pneumoniae pada luka DM dengan kultur. Ditemukannya bakteri Klebsiella pneumoniae pada luka DM. Dari 28 sampel swab luka DM yang dilakukan di klinik pengobatan diabetes, Kota Pontianak dapat ditemukan 20 sampel positif Klebsiella pneumoniae (71,42%) dan 8 sampel lainnya negatif Klebsiella pneumoniae (28,57%). Kata Kunci: Bakteri Gram-Negatif, Diabetes Mellitus, Luka
Co-Authors Agitya Cita Amanda Ananda, Fanny Rizki Ari Nuswantoro Aufani, Destia Azzahra, Larasabella Bagus Muhammad Ihsan Deara Zanika Intan Pristanty Destia Aufani Dinasti Aprillia Djohan, Herlinda Etiek Nurhayati Farwa, Syalsabilla Furwanti, Endang Sri Hendra Budi Sungkawa Hendra Budi Sungkawa, Hendra Budi Herlinda Djohan Ihsan, Bagus Ihsan, Bagus Muhammad Ikhsan, Bagus Muhammad Labunda, Aura Iqlima Laila Kamilla Maretalinia Maretalinia Maretalinia, Maretalinia Merda Sri Rahayu Mikrani, Ashfaque Raza Nisa Kartika Komara Nurisma, Yuni Pratiwi, Tarisya Fitri Qatrunada, Putri Norfa Rahayu, Merda Sri Roofiif, Sayyid Al Sari, Emilda Slamet . Sri Tumpuk Supriyanto Supriyanto Supriyanto Supriyanto Sutriswanto, Sutriswanto Suwandi, Edy Sya’baniar, Luthfiranda Syopingi Syopingi Syopingi, Syopingi Tumpuk, Sri Ulfia, Phisa Isyah Wahdaniah Wahyudi