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Potential of Garlic Filtrate as An Alternative Anticoagulant for Whole Blood Samples Ari Nuswantoro; Jessica Ningtyas Berlianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2683

Abstract

Synthetic anticoagulants such as heparin, citric, and ethylenediaminetetraacetic acid (EDTA) are commonly used to prevent blood clots. In contrast, its widespread use in clinical laboratories is still constrained by price, its toxic nature, and its short shelf life. Therefore, an alternative material that is relatively cheap, non-toxic, and easy to obtain and process in a ready-to-use form is needed. Garlic contains allicin and ajoene, which are anti-platelet and anti-thrombogenic. This study’s aim is to explore the potential of garlic filtrate as an alternative anticoagulant.  Blood from 16 individuals was used and separated into four groups: non-anticoagulant, 50 μL/mL garlic filtrate, 100 μL/mL garlic filtrate, and 150 μL/mL heparin, for a total of 64 treatments. The Lee and White method showed that non-anticoagulated blood had normal clotting times (mean 8 minutes and 56 seconds), whereas heparin plasma and garlic filtrate plasma had longer clotting times (more than 20 minutes); and this is statistically different based on the ANOVA test with a significance value (p) of 0.000 < 0.05. On spectrophotometric measurements, the levels of calcium ions in heparinized plasma and serum were 8.66 mg/dL and 8.52 mg/dL, respectively, while in garlic plasma filtrate of 50 μL/mL and 100 μL/mL were 4.13 mg/dL and 3.58 mg/dL, respectively; this is also statistically different based on the ANOVA test with a significance value of 0.000 < 0.05. The differences indicate that garlic filtrate can extend clotting time and reduce calcium ions therefore it is worth reviewing as an alternative anticoagulant.
Pengaruh Penambahan Lidah Buaya (Aloe barbadensis Miller) pada Minyak Goreng Bekas terhadap Kadar Bilangan Asam Maulidiyah Salim; Gervacia Jenny Ratnawati; Ari Nuswantoro
Jurnal Vokasi Kesehatan Vol 7, No 1 (2021): JANUARI 2021
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.639 KB) | DOI: 10.30602/jvk.v7i1.833

Abstract

Minyak goreng bekas memiliki kadar bilangan asam yang tinggi dan dapat menyebabkan kerusakan minyak tersebut. Tujuan dari penelitian ini adalah untuk mengetahui apakah ada pengaruh penambahan lidah buaya (Aloe barbadensis Miller) pada minyak goreng bekas terhadap kadar bilangan asam. Desain penelitian yang digunakan adalah eksperimen semu, menggunakan Teknik purposive sampling. Sampel adalah minyak goreng yang telah mengalami proses penggorengan secara berulang yang dibagi menjadi ke dalam 4 kelompok perlakuan dan masing-masing 6 pengulangan sehingga total sampel menjadi 24. Kadar asam lemak bebas diperiksa dengan menggunakan metode alkalimetri. Nilai rata-rata kadar bilangan asam pada kelompok yang terdiri dari tanpa penambahan lidah buaya, dengan penambahan lidah buaya 25 gram, dengan penambahan lidah buaya 50 gram, dan dengan penambahan lidah buaya 75 gram berturut-turut adalah 0,17%, 0,15%, 0,11%, dan 0,09%.  Hasil uji regresi linier sederhana memberikan nilai probabilitas 0,000 (p < 0,05) sehingga dinyatakan ada pengaruh penambahan lidah buaya pada minyak goreng bekas terhadap kadar bilangan asam.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN KITOLOD (Isotoma longiflora) TERHADAP PERTUMBUHAN BAKTERI Streptococcus pyogenes Ari Nuswantoro; Kartini Kartini
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3343

Abstract

Kitolod is a plant that is easy to grow in the tropics, including Indonesia. Kitolod leaves contain flavonoids, alkaloids, tannins, saponins and terpenoids that have the potential to be used as antibacterial agents to inhibit the growth of pathogenic bacteria such as Streptococcus pyogenes. This study aims to determine the antibacterial activity of kitolod leaf extract on the growth of Streptococcus pyogenes. This research is quasi-experimental with purposive sampling technique. Kitolod leaves were extracted by maceration method using ethanol then dissolved in 15% DMSO to obtain concentrations of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, and 90%. Each concentration was replicated three times, and the control used the antibiotic bacitracin. After carrying out the antimicrobial sensitivity test using the Kirby-Bauer diffusion method, the results obtained were inhibition zones of 17.66 mm (sensitive), 18.16 mm (sensitive), 18.50 mm (sensitive), 19 mm (sensitive), 19. 83 mm (sensitive), 21.16 mm (sensitive), 21.83 mm (sensitive), 22.33 mm (sensitive), and 23.83 mm (sensitive) respectively, while the bacitracin control was 17.5 mm (sensitive). Thus, kitolod leaf extract proved to have antibacterial activity against the growth of Streptococcus pyogenes.
Identifikasi Bakteri Coliform pada Minuman Air Tahu dan Air Tebu yang Dijual di Wilayah Kota Pontianak Ramadan, Aditiya; Jordan, Aristo; Ardhani, Adha Riesta; Monalita, Ria; Munardi, Fhatia Natasya; Syahdilla, Ade; Nuswantoro, Ari; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1470

Abstract

Air tahu dan air tebu merupakan minuman manis dan menyegarakan yang cukup diminati oleh masyarakat Kota Pontianak terutama dari kalangan anak-anak, remaja, dan orang tua. Cemaran bakteri Coliform yang terdapat pada minuman air tahu dan air tebu yang dijual di Kota Pontianak dapat menyebabkan masalah serius pada kesehatan. Tujuan dari penelitian ini adalah untuk mengetahui adanya cemaran bakteri Coliform pada minuman air tahu dan air tebu yang dijual di pinggir jalan Kota Pontianak dengan menggunakan metode kultur. Desain penelitian ini adalah penelitian deskriptif dengan pengambilan sampel secara total sampling. Penelitian ini dilakukan dengan cara mengambil sebanyak 43 sampel air tahu dan 70 sampel air tebu yang dijual di pinggir jalan di Kota Pontianak. Hasil penelitian dari 43 sampel air tahu menunjukkan 17 sampel (39,5%) positif bakteri Coliform, 26 sampel lainnya (60,5%) negatif bakteri Coliform, dan hasil peneilitan dari 70 sampel air tebu menunjukkan 37 sampel (52,8%) positif bakteri Coliform, dan 33 sampel lainnya (47,2%) negatif Coliform.
PROFILE OF RENAL FUNCTION LABORATORY EXAMINATIONS IN PROLANIS PATIENTS IN PONTIANAK CITY Nuswantoro, Ari; Aprillia, Dinasti; Purwaningsih, Indah; Arifah, Rizki Zanuba
Jurnal Laboratorium Khatulistiwa Vol 5, No 2 (2022): Mei 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v5i2.979

Abstract

Diabetes mellitus (DM) and hypertension is one of the chronic diseases that cause health problems in Indonesia. The government organizes a Management Program Chronic Disease (Prolanis) in order to manage patients with both of these diseases. One of the activities carried out is laboratory tests of urea and serum creatinine. This study aims to describe the number of DM and hypertension sufferers as well as serum urea and creatinine levels in Prolanis participants which checked themselves into the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. The study was conducted using a total sampling method by looking at the medical record data of Prolanis participants, who will check themselves to the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak, from January – December 2021 so that data were obtained from 333 patients consisting of 84 men and 349 women with an age range of 26 – 91 years. The results showed that 163 patients were diagnosed with DM and the remaining 170 people suffered from hypertension. The average value of urea levels to male patients is 36.4 mg/dL (14 – 113 mg/dL) and to women it is 27.3 mg/dL (11 – 80 mg/dL). The average value of creatinine levels to men is 1.3 mg/dL (0.5 – 5.3 mg/dL) and to women is 0.8 mg/dL (0.4 – 1.9 mg/dL). Urea and creatinine levels are theoretically associated with the incidence of DM and hypertension.
Pengukuran Nilai MPN Pada Air Minum Isi Ulang Di Wilayah Kota Pontianak Yang Disterilisasi Dengan Portabel Ultraviolet Dan Portabel Filtrasi Reza, Nur; Nuswantoro, Ari; Indrawati, Ratih; Muhammad Ihsan, Bagus
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Air merupakan kebutuhan utama manusia untuk bertahan hidup sedangkan sumber air bersih alami semakin banyak yang tercemar. Sebagai alternatif, masyarakat mendapatkan air minum dari depot air minum isi ulang (DAMIU) yang menggunakan metode sterilisasi portabel ultraviolet (UV) dan portabel filtrasi. Namun demikian, bakteri coliform masih ditemukan pada air minum karena air baku yang tercemar, alat yang digunakan tidak steril, dan minimnya wawasan masyarakat mengenai sanitasi dan higien DAMIU. Tujuan penelitian ini menghitung nilai most probable number (MPN) pada DAMIU di Kota Pontianak. Penelitian ini berbentuk deskriptif eksploratif berdesain cross-sectional. Data diperoleh dengan melakukan pemeriksaan MPN terhadap 36 sampel DAMIU. Hasil menunjukkan 14 (77,78%) DAMIU metode portabel ultraviolet dan 17 DAMIU (94,44%) portabel filtrasi menunjukkan nilai MPN di atas batas yang ditentukan. Banyaknya DAMIU yang tidak sesuai persyaratan kualitas air minum layak konsumsi sesuai Permenkes menunjukkan adanya risiko penularan penyakit yang disebabkan oleh coliform melalui air minum DAMIU di Kota Pontianak.
POTENSI TEPUNG KULIT PISANG RAJA SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Penicillium sp. Kamilla, Laila; Fadilla, Alda Nur; Nuswantoro, Ari; Tumpuk, Sri
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1665

Abstract

Sumber karbohidrat yang mudah ditemukan dan belum banyak dimanfaatkan adalah tepung kulit pisang raja. Kulit pisang raja memiliki potensi untuk digunakan sebagai media alternatif karena memiliki kandungan zat seperti karbohidrat, protein, dan lemak. Tujuan penelitian ini untuk mengetahui potensi tepung kulit pisang raja sebagai media pertumbuhan jamur Penicillium sp. Penelitian ini menggunakan desain penelitian Quasi Experiment. Populasi dalam penelitian ini yaitu kulit pisang raja. Sampel yang digunakan adalah tepung kulit pisang raja yang dibuat dalam konsentrasi 10%, 20%, 30%, 40%, 50% dengan 5 kali pengulangan didapatkan jumlah sampel 25. Hasil penelitian yaitu konsentrasi 10% rata- rata 11,6 CFU, konsentrasi 20% rata-rata 12,8 CFU, konsentrasi 30% rata-rata 17,4 CFU, konsentrasi 40% rata-rata 21,8 CFU, konsentrasi 50% rata-rata 28 CFU dan media standar Sabouraud Dextrose Agar (SDA) koloni diperoleh rata-rata 152,6 CFU. Hasil uji Anova didapatkan nilai 0,000 < 0,05 yang menyatakan ada perbedaan pertumbuhan jumlah koloni pada tiap konsentrasi media alternatif dengan media standar Sabouraud Dextrose Agar (SDA) dapat disimpulkan media alternatif tepung kulit pisang raja berpotensi digunakan sebagai media pertumbuhan jamur Penicillium sp. namun tidak dapat menggantikan media standar Sabouraud Dextrose Agar (SDA).
PERBEDAAN HASIL HITUNG BERBAGAI JENIS LEUKOSIT PADA PASIEN COVID-19 YANG ISOLASI MANDIRI DENGAN ISOLASI DI RSUD KABUPATEN MELAWI Reza, Nur; Santoso, Edi; Nuswantoro, Ari; Indrawati, Ratih; Nurhayati, Etiek; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 3 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i3.1886

Abstract

COVID-19 infection causes various clinical manifestations so the treatment method is also different for each individual. Patient isolation is one of the treatments to break the chain of transmission of COVID-19. Asymptomatic to moderate symptomatic patients are usually asked to self-isolate, while those with severe and critical symptoms are isolated in hospital. Leukocyte examination is an important parameter and plays a role in determining patient follow-up. The aim of this study was to find out whether there were differences in leukocyte differential-counts in COVID-19 patients who were self-isolating and those isolated at Melawi Regional Hospital. This study had a cross-sectional design by taking data from medical records of COVID-19 patients at Melawi Regional Hospital during October 2021 to July 2022. The data taken were from 399 COVID-19 patients, consisting of 182 patients are self-isolating and 217 patients are isolating at Melawi Regional Hospital. In self-isolated patients, the average value of leukocyte count was 7.04 x 10³ cells/µL, and the leukocyte differential-counts was neutrophils 58.45%, lymphocytes 21.81%, monocytes 13.84%, eosinophils 1.52%, and basophils 4.38%. In isolated patients at Melawi Regional Hospital, the average value of leukocyte count was 9.89 x 10³ cells/µL, and the leukocyte differential-counts was neutrophils 75.09%, lymphocytes 12.97%, monocytes 8.38%, eosinophils 0.46%, and basophils 3.11%. The Mann-Whitney U test obtained a significance value of 0.000 (p < 0.05), which means there is a significant difference in the results of leukocyte counts between self-isolated patients and those isolated at Melawi Regional Hospital.
KAJIAN TENTANG PENURUNAN JUMLAH DONOR DARAH AKIBAT PANDEMI COVID-19: DATA SELAMA 4 TAHUN DARI UDD PMI KOTA PONTIANAK Aditia, Aditia; Samwidya, Veronika Sriayu; Nuswantoro, Ari; Djohan, Herlinda; Supriyanto, Supriyanto; Reza, Nur
Media Penelitian dan Pengembangan Kesehatan Vol. 32 No. 4 (2022): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v32i4.1890

Abstract

The COVID-19 pandemic has left a noticeable mark on the health care sector. One such example is the blood services provided by the Blood Donor Unit (UDD) of Indonesian Red Cross (PMI). Every time there was an increase in COVID-19 cases, blood donation activities sharply decreased, according to the UDD PMI. During the COVID-19 pandemic, there was a scarcity of blood donors. This research aimed to see the difference in the number of blood donors before and during the pandemic. This research was descriptive analytical with a cross-sectional design by collecting data on the number of blood donors at UDD PMI Pontianak City from 2018 to 2022. The results of the study show that the number of blood donors before the pandemic was 52,607 donors, while the number of blood donors during the pandemic was 48,421 donors. which means there was a decrease in the number of blood donors by 4,186 donors (7.96%). The Independent Samples Test obtained a significant value of 0.045 (p < 0.05), which means there was a difference in the number of blood donors before and during the COVID-19 pandemic at UDD PMI Pontianak City. Concerns about the risk of infection and government policies limiting activities outside the home can explain why the COVID-19 pandemic has resulted in a decline in blood donation rates.
KORELASI KADAR C-REACTIVE PROTEIN DENGAN JUMLAH TROMBOSIT PADA PENDERITA DIABETES MELITUS TIPE 2 Nuswantoro, Ari; Wicaksono, Didik; Aditia, Aditia
Media Penelitian dan Pengembangan Kesehatan Vol. 33 No. 2 (2023): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v33i2.1903

Abstract

Type 2 diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia and insulin resistance, associated with decreased nitric oxide (NO) levels, increased free radical production, inflammation, endothelial dysfunction, and progressive vascular damage. C-reactive protein (CRP), an acute phase protein regulated by pro-inflammatory cytokines like interleukin-6 (IL-6), serves as a systemic inflammation marker. Pentameric CRP (pCRP) and monomeric CRP (mCRP) are implicated in mediating inflammation and endothelial dysfunction, with roles in endothelial cell adhesion, phagocytosis, inflammatory signaling, and interactions with endothelial cells, neutrophils, macrophages, and platelets. Additionally, leukocytes secrete platelet-activating factors, enhancing platelet activation and cytokine and chemokine release. This descriptive analytical study with a cross-sectional design aimed to investigate the correlation between CRP levels and platelet counts in patients with type 2 DM. Utilizing purposive sampling, 47 patients diagnosed with DM at Sultan Syarif Mohamad Alkadrie Hospital, Pontianak, during June 2023 were selected. CRP levels were measured using the fluorescence immunoassay (FIA) method, while platelet counts were determined through the impedance method. Statistical analysis employing Kendall's Tau-b test revealed a significant correlation (p < 0.05, r = -0.508), indicating that elevated CRP levels are significantly associated with reduced platelet counts in type 2 DM patients.
Co-Authors Adawiyah, Rabi’atul Aditia Aditia Aditia, Aditia Affifatuzahara Affifatuzahara Affifatuzahara, Affifatuzahara Al-qorifah, Fahma Ajeng Alfianita, Riska Anisya Nur Shafitri Anjelica, Benedikta Aprilia, Dinasti Ardhani, Adha Riesta Arifah, Rizki Zanuba Artanti, Lina Yunda Astuti, Fitri Mega Aufani, Destia Aulia, Annisa Syafa Azura, Azura Azzahra, Larasabella Bagus Muhammad Ihsan Benedikta Anjelica Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprillia Djohan, Herlinda Edi Santoso Emilda Sari Fadilla, Alda Nur Fahma Ajeng Al-qorifah Fahrani, Chantika Lailatussa'adah Fitra Fathuhudin Grovindo Grovindo Hartati, Fitri Ulya Hendra Budi Sungkawa, Hendra Budi Herlinda Djohan Ihsan, Bagus Muhammad Imma Fatayati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Jessica Ningtyas Berlianti Jordan, Aristo Juliana Cristyaningsih Kartini Kartini Laila Kamilla Laila Kamilla Linda Triana Linda Triana Liza Harum Nur Annisa Maretalinia Maretalinia Maretalinia, Maretalinia Maulidiyah Salim Merda Sri Rahayu Monalita, Ria Muhammad Ihsan, Bagus Munardi, Fhatia Natasya Natasya Intan Ramadhani Natasya Intan Ramadhani Nurhayati, Etiek Nurul Uq'ma P Purwanto Panyada Cholsakhon Perdede, Lusi Hernawati Pratiwi, Tarisya Fitri Rahayu, Merda Sri Ramadan, Aditiya Ratnawaty, Gervacia Jenny Ratulangi, Agus Dimas Reza Setiawan Reza, Nur Rezky, Muhammad Andyka Riska Alfianita Rizki Zanuba Arifah Rizki, Sri Yuniarti Romadi Romadi Sadid, Randy Samwidya, Veronika Sriayu Sen, Sharen Shafitri, Anisya Nur Slamet Slamet Sri Tumpuk Sugito Sugito Sugito Sugito Supriyanto Supriyanto Sutriswanto Sutriswanto Suwandi, Edy Syahdilla, Ade Syopingi Syopingi Syopingi, Syopingi Tantri Rahmatika Theresia Indah Budhy Sulisetyawati Tika, Dian Tumpuk, Sri Uq'ma, Nurul Veny Veny Wahdaniah Wahyudi Wicaksono, Didik Yunus, Mohd