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All Journal Journal Of Vocational Health Studies Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Ners Jurnal teknologi Kesehatan Borneo MAHESA : Malahayati Health Student Journal Jurnal EduHealth JURNAL ILMIAH GLOBAL EDUCATION Innovative: Journal Of Social Science Research Jurnal Vokasi Kesehatan Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Media Penelitian dan Pengembangan Kesehatan MEDICA (International Medical Scientific Journal) Jurnal Info Kesehatan ELECTRON (Journal of Science and Technology)
Ari Nuswantoro
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
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Potential of Garlic Filtrate as An Alternative Anticoagulant for Whole Blood Samples Ari Nuswantoro; Jessica Ningtyas Berlianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2683

Abstract

Synthetic anticoagulants such as heparin, citric, and ethylenediaminetetraacetic acid (EDTA) are commonly used to prevent blood clots. In contrast, its widespread use in clinical laboratories is still constrained by price, its toxic nature, and its short shelf life. Therefore, an alternative material that is relatively cheap, non-toxic, and easy to obtain and process in a ready-to-use form is needed. Garlic contains allicin and ajoene, which are anti-platelet and anti-thrombogenic. This study’s aim is to explore the potential of garlic filtrate as an alternative anticoagulant.  Blood from 16 individuals was used and separated into four groups: non-anticoagulant, 50 μL/mL garlic filtrate, 100 μL/mL garlic filtrate, and 150 μL/mL heparin, for a total of 64 treatments. The Lee and White method showed that non-anticoagulated blood had normal clotting times (mean 8 minutes and 56 seconds), whereas heparin plasma and garlic filtrate plasma had longer clotting times (more than 20 minutes); and this is statistically different based on the ANOVA test with a significance value (p) of 0.000 < 0.05. On spectrophotometric measurements, the levels of calcium ions in heparinized plasma and serum were 8.66 mg/dL and 8.52 mg/dL, respectively, while in garlic plasma filtrate of 50 μL/mL and 100 μL/mL were 4.13 mg/dL and 3.58 mg/dL, respectively; this is also statistically different based on the ANOVA test with a significance value of 0.000 < 0.05. The differences indicate that garlic filtrate can extend clotting time and reduce calcium ions therefore it is worth reviewing as an alternative anticoagulant.
Combination of Aloe Vera and Chocolate to Reduce The Number of The Colony of Staphylococcus Aureus Edy Suwandi; Ari Nuswantoro; Sugito Sugito
Jurnal teknologi Kesehatan Borneo Vol 3 No 2 (2022): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v3i2.197

Abstract

Aloe vera (AV) and chocolate are good examples of functional foods because they contain prebiotics that can help the host defend itself against harmful bacterial infections such as Staphylococcus aureus. Many studies have used AV or chocolate alone to help fight infection, but not much is known about using both together. This study aims to count the number of colonies of S. aureus infected on mice’s skin (Mus musculus) after being given AV and cocoa in certain doses per kilogram of body weight. A total of 35 healthy male mice were infected with S. aureus in the superficial area of ​​the nape of the skin. After that, they were divided into seven groups, each consisting of 5 mice, and given the following treatments: given 50 mg/kg BW AV (group A), 50 mg/kg BW brown (B), 50 mg/kg BW combination of AV and chocolate (C), given 75 mg/kg BW AV (D), given 75 mg/kg BW brown (E), given 75 mg/kg BW combination of AV and chocolate (F), and control (without prebiotic administration), K). Mice that were given 75 mg/kg BW AV/chocolate/AV+chocolate had a better ability to reduce the number of bacteria than those that were given 50 mg/kg BW AV/chocolate/AV+chocolate. In addition, rats that were given AV and chocolate together had a better ability to reduce the number of bacteria than those that were given either only AV or chocolate alone. Prebiotics are both found in AV and chocolate but with different substances. This causes mice given a combination of prebiotics to be considered better than those given only one type of prebiotic source.
Pengaruh Penambahan Lidah Buaya (Aloe barbadensis Miller) pada Minyak Goreng Bekas terhadap Kadar Bilangan Asam Maulidiyah Salim; Gervacia Jenny Ratnawati; Ari Nuswantoro
Jurnal Vokasi Kesehatan Vol 7, No 1 (2021): JANUARI 2021
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.639 KB) | DOI: 10.30602/jvk.v7i1.833

Abstract

Minyak goreng bekas memiliki kadar bilangan asam yang tinggi dan dapat menyebabkan kerusakan minyak tersebut. Tujuan dari penelitian ini adalah untuk mengetahui apakah ada pengaruh penambahan lidah buaya (Aloe barbadensis Miller) pada minyak goreng bekas terhadap kadar bilangan asam. Desain penelitian yang digunakan adalah eksperimen semu, menggunakan Teknik purposive sampling. Sampel adalah minyak goreng yang telah mengalami proses penggorengan secara berulang yang dibagi menjadi ke dalam 4 kelompok perlakuan dan masing-masing 6 pengulangan sehingga total sampel menjadi 24. Kadar asam lemak bebas diperiksa dengan menggunakan metode alkalimetri. Nilai rata-rata kadar bilangan asam pada kelompok yang terdiri dari tanpa penambahan lidah buaya, dengan penambahan lidah buaya 25 gram, dengan penambahan lidah buaya 50 gram, dan dengan penambahan lidah buaya 75 gram berturut-turut adalah 0,17%, 0,15%, 0,11%, dan 0,09%.  Hasil uji regresi linier sederhana memberikan nilai probabilitas 0,000 (p < 0,05) sehingga dinyatakan ada pengaruh penambahan lidah buaya pada minyak goreng bekas terhadap kadar bilangan asam.
Lipid Profile of Prolanis Patients in Pontianak City Ari Nuswantoro; Dinasti Aprillia; Juliana Cristyaningsih
Jurnal teknologi Kesehatan Borneo Vol 4 No 1 (2023): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v4i1.209

Abstract

Perubahan lingkungan, teknologi dan gaya hidup memicu naiknya kasus penyakit tidak menular, seperti diabetes melitus (DM) dan hipertensi (HT), yang bersifat kronis dan membawa risiko kematian terutama di negara berkembang seperti Indonesia. Dua penyakit tersebut dapat dideteksi dengan menilai profil lipid individu dimana kondisi dislipidemia dapat dianggap mengarah pada risiko terjadinya penyakit kardiovaskuler. Pemerintah Indonesia meluncurkan program Prolanis terhadap pasien DM dan HT yang salah satu kegiatannya adalah pemeriksaan profil lipid yang meliputi kadar kolesterol, trigliserida, HDL-kolesterol, dan LDL-kolesterol. Dari hasil pemeriksaan ini dapat dinilai rasio trigliserida terhadap HDL-kolesterol, yang merupakan prediktor paling kuat terhadap penyakit kardiovaskuler. Penelitian ini mengambil data pemeriksaan profil lipid dari 337 pasien Prolanis di Kota Pontianak. Hasil penelitian menunjukkan bahwa pasien DM didominasi oleh orang dengan hiperkolesterolemia (63,03%), trigliserida normal (56,36%), HDL-kolesterol normal (91,51%), LDL-kolesterol tinggi (83,03%), dan rasio trigliserida/HDL-kolesterol normal (60%). Demikian pula pada pasien HT, hiperkolesterolemia dan LDL-kolesterol tinggi dialami sebagian besar pasien (69,76 dan 84,30%), sedangkan trigliserida normal, HDL-kolesterol normal, dan rasio Trigliserida/HDL-kolesterol normal lebih mendominasi dengan persentase 61,61%, 94,19%, dan 68,03%. Hasil penelitian ini menunjukkan bahwa baik pasien DM maupun HT memiliki kecenderungan profil lipid yang normal dan menuju dislipidemia.
IDENTIFIKASI DAN PROFIL RESISTENSI BAKTERI PADA MINUMAN AIR TAHU DAN AIR TEBU DI WILAYAH KOTA PONTIANAK Aditia Aditia; Ari Nuswantoro; Herlinda Djohan; Bagus Muhammad Ihsan; Nurul Uq'ma; Anisya Nur Shafitri; Affifatuzahara Affifatuzahara; Fahma Ajeng Al-qorifah; Benedikta Anjelica
Jurnal Ilmiah Global Education Vol. 4 No. 4 (2023): JURNAL ILMIAH GLOBAL EDUCATION, Volume 4 Nomor 4, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/jige.v4i4.1860

Abstract

Tofu and sugarcane juice drinks are in great demand because they taste delicious and affordable. These drinks can be contaminated with pathogenic bacteria due to hygiene and poor environment. Contamination of pathogenic bacteria that are resistant to antibiotics is a serious health threat.  The objective of this study is to identify and provide antibiotic resistance bacteria from tofu and sugarcane juice drinks overview. This Study is a descriptive-observation with a cross-sectional design. Bacterial identification is carried out by culture, while resistance testing is carried out by Kirby-Bauer. Penicillin, amoxicillin, ampicillin, ciprofloxacin, chloramphenicol, gentamicin, tetracycline, and vancomycin were used. 43 samples of tofu water drink and 70 samples of sugarcane juice drink were selected by purposive sampling. Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes, and Klebsiella pneumoniae founds in tofu drinks, while Enterobacter aerogenes, Salmonella typhi, Shigella dysenteriae, and Escherichia coli founds in sugarcane juice. Staphylococcus aureus resistant to penicillin (100%), Salmonella typhi resistant to penicillin (66.6%), Enterobacter aerogenes resistant to tetracycline (70%), Klebsiella pneumoniae resistant to penicillin (100%), Shigella dysenteriae resistant to ampicillin and gentamicin (100%), and Escherichia coli resistant to vancomycin (100%). Pathogenic-resistant bacteria in tofu and sugarcane juice drinks show the risk of spreading resistant bacteria which can trigger bacterial resistance with a wider spectrum.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN KITOLOD (Isotoma longiflora) TERHADAP PERTUMBUHAN BAKTERI Streptococcus pyogenes Ari Nuswantoro; Kartini Kartini
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3343

Abstract

Kitolod is a plant that is easy to grow in the tropics, including Indonesia. Kitolod leaves contain flavonoids, alkaloids, tannins, saponins and terpenoids that have the potential to be used as antibacterial agents to inhibit the growth of pathogenic bacteria such as Streptococcus pyogenes. This study aims to determine the antibacterial activity of kitolod leaf extract on the growth of Streptococcus pyogenes. This research is quasi-experimental with purposive sampling technique. Kitolod leaves were extracted by maceration method using ethanol then dissolved in 15% DMSO to obtain concentrations of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, and 90%. Each concentration was replicated three times, and the control used the antibiotic bacitracin. After carrying out the antimicrobial sensitivity test using the Kirby-Bauer diffusion method, the results obtained were inhibition zones of 17.66 mm (sensitive), 18.16 mm (sensitive), 18.50 mm (sensitive), 19 mm (sensitive), 19. 83 mm (sensitive), 21.16 mm (sensitive), 21.83 mm (sensitive), 22.33 mm (sensitive), and 23.83 mm (sensitive) respectively, while the bacitracin control was 17.5 mm (sensitive). Thus, kitolod leaf extract proved to have antibacterial activity against the growth of Streptococcus pyogenes.
Resistance Profile Antibiotics Pathogenic Bacteria from SWAB Wounds of Pontianak City Diabetes Treatment Clinic Patients Azura azura; Sri Tumpuk; Ari Nuswantoro
Jurnal EduHealth Vol. 14 No. 04 (2023): Jurnal eduHealt, 2023, December
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes mellitus (DM) is a type of metabolic disorder where the sufferer has high blood sugar levels due to the body's failure to respond or produces insufficient amounts of insulin, or a metabolic disorder caused by hyperglycemia due to abnormalities in insulin secretion and insulin action or both. One of the complications of diabetes is ulcers, where a superficial infection occurs on the sufferer's skin and becomes a strategic location for bacterial growth. Most of the drugs that are widely used to inhibit or kill bacteria that cause infections in humans are antibiotics which carry the risk of drug resistance. Antibiotic resistance is a growing problem in many parts of the world. Antibiotic resistance can occur due to inappropriate or excessive use of antibiotics. This study aims to determine the susceptibility of pathogenic bacteria to several antimicrobial agents or antibiotics and to determine the percentage of antibiotic resistance in diabetes mellitus patients who experience complications, which will make it easier to choose the right antibiotic for healing. This research uses a descriptive method using samples of bacterial isolates which have been identified as 11 samples of Staphylococcus aureus bacterial isolates, 11 samples of Pseudomonas aeruginosa isolates and 39 samples of Klebsiella pneumoniae bacterial isolates. Antimicrobial susceptibility test (AST) disk diffusion method (Kirby & Bauer test) using Mueller Hinton media. With eight different types of antibiotics, this test was carried out to prove antimicrobial activity by measuring the diameter of the antimicrobial activity inhibition zone. Resistance test results were obtained in Staphylococcus aureus experiencing the highest resistance to the antibiotic Gentamycin (CN) at 63.63%, in Pseudomonas aeruginosa experiencing high resistance to the antibiotic Ampicillin (AMP) at 54.54%, and in Klebsiella pneumoniae experiencing the highest resistance. against the antibiotic Ampicillin (AMP) was 41.02%. Preventing antibiotic resistance in wound healing is very important to ensure antibiotics remain effective in treating infections. Therefore, this research was carried out in order to determine the correct type of antibiotic for healing wounds in diabetes mellitus sufferers.
Normal dan Dislipidemia: Profil Lipid Pasien Hipertensi- Prolanis di Kota Pontianak Sharen Sen; Ari Nuswantoro; Laila Kamilla; Dinasti Aprilia
MAHESA : Malahayati Health Student Journal Vol 4, No 1 (2024): Volume 4 Nomor 1 (2024)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v4i1.13266

Abstract

ABSTRACT The primary cause of cardiovascular disease (CVD) is hypertension (HT). Through the Prolanis program, BPJS treats HT patients in Indonesia through various activities, including physical activity and monitoring lipid profiles, which until now have not been widely reported. The aim of this study was to describe the lipid profile of HT patients participating in Prolanis in Pontianak City. The samples are the medical records from 110 patients chosen using the total sampling method in 2022 at the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. Information gathered from 99 women and 11 men revealed that 80.8% of the women had hypercholesterolemia; triglycerides and HDL were mainly within normal ranges; LDL ranged from normal (13.6%) to extremely high (8.2%); and the ratios of cholesterol to HDL and triglycerides to HDL were mainly normal. One of the Prolanis program's goals, physical exercise, appears to have an impact on the patient's typical lipid profile. Nonetheless, the fact that some people have dyslipidemia raises awareness of the risk of CVD and supports the necessity of keeping this program in place. Keywords: Cardiovascular Disease, Lipid Profile, Hypertensive Patients, Prolanis  ABSTRAK Penyebab utama penyakit kardiovaskular (CVD) adalah hipertensi (HT). Melalui program Prolanis, BPJS menangani pasien HT di Indonesia melalui berbagai kegiatan, termasuk aktivitas fisik dan pemantauan profil lipid, yang hingga saat ini belum banyak dilaporkan. Tujuan dari penelitian ini adalah untuk mendeskripsikan profil lipid pasien HT yang mengikuti Prolanis di Kota Pontianak. Sampel penelitian ini adalah rekam medis dari 110 pasien yang dipilih dengan menggunakan metode total sampling pada tahun 2022 di Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. Informasi yang dikumpulkan dari 99 wanita dan 11 pria menunjukkan bahwa 80,8% wanita mengalami hiperkolesterolemia; trigliserida dan HDL sebagian besar dalam kisaran normal; LDL berkisar dari normal (13,6%) hingga sangat tinggi (8,2%); dan rasio kolesterol terhadap HDL serta trigliserida terhadap HDL sebagian besar dalam kisaran normal. Salah satu tujuan program Prolanis, yaitu latihan fisik, tampaknya berdampak pada profil lipid pasien. Meskipun demikian, fakta bahwa beberapa orang memiliki dislipidemia meningkatkan kesadaran akan risiko CVD dan mendukung perlunya mempertahankan program ini. Kata Kunci: Penyakit Kardiovaskular, Profil Lipid, Pasien Hipertensi, Prolanis
Gambaran Kadar Glukosa Darah Sewaktu Pada Ibu Hamil di Puskesmas Tambelan Sampit Kota Pontianak Aditiya Ramadan; Ari Nuswantoro; Linda Triana; Bagus Muhammad Ihsan
Jurnal Ners Vol. 8 No. 1 (2024): APRIL 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v8i1.22203

Abstract

characterised by high blood sugar levels as well as problems with protein, lipid, and carbohydrate metabolism as a result of inadequate insulin activity. Patients who have wounds infected by pathogenic bacteria, both Gram positive and Gram negative, run the risk of developing a number of problems, including neuropathy, hypertension, coronary heart disease, retinopathy, nephropathy, and gangrene. This cross-sectional, observational, descriptive study aims to pinpoint pathogenic bacteria in the diabetic patients' wounds at the diabetes wound care clinic in Pontianak City. The bacterial identification stage includes inoculation on enrichment media, Gram staining, inoculation and observation of colonies on differential-selective media, and biochemical tests. Examination results showed that Klebsiella pneumoniae was identified in 20 samples (57%), Pseudomonas aeruginosa in 8 samples (23%), and Staphylococcus aureus in 7 samples (20%). According to the study, diabetic wound problems put DM patients at risk of contracting dangerous microorganisms and spreading them to others. In order to prevent infection and other health issues, patients are instructed on how to properly care for their wounds. They are also provided the correct follow-up by medical professionals.
Identifikasi Bakteri Coliform pada Minuman Air Tahu dan Air Tebu yang Dijual di Wilayah Kota Pontianak Ramadan, Aditiya; Jordan, Aristo; Ardhani, Adha Riesta; Monalita, Ria; Munardi, Fhatia Natasya; Syahdilla, Ade; Nuswantoro, Ari; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1470

Abstract

Air tahu dan air tebu merupakan minuman manis dan menyegarakan yang cukup diminati oleh masyarakat Kota Pontianak terutama dari kalangan anak-anak, remaja, dan orang tua. Cemaran bakteri Coliform yang terdapat pada minuman air tahu dan air tebu yang dijual di Kota Pontianak dapat menyebabkan masalah serius pada kesehatan. Tujuan dari penelitian ini adalah untuk mengetahui adanya cemaran bakteri Coliform pada minuman air tahu dan air tebu yang dijual di pinggir jalan Kota Pontianak dengan menggunakan metode kultur. Desain penelitian ini adalah penelitian deskriptif dengan pengambilan sampel secara total sampling. Penelitian ini dilakukan dengan cara mengambil sebanyak 43 sampel air tahu dan 70 sampel air tebu yang dijual di pinggir jalan di Kota Pontianak. Hasil penelitian dari 43 sampel air tahu menunjukkan 17 sampel (39,5%) positif bakteri Coliform, 26 sampel lainnya (60,5%) negatif bakteri Coliform, dan hasil peneilitan dari 70 sampel air tebu menunjukkan 37 sampel (52,8%) positif bakteri Coliform, dan 33 sampel lainnya (47,2%) negatif Coliform.
Co-Authors Adawiyah, Rabi’atul Aditia Aditia Aditia, Aditia Aditiya Ramadan Affifatuzahara Affifatuzahara Anisya Nur Shafitri Ardhani, Adha Riesta Arifah, Rizki Zanuba Astuti, Fitri Mega Aufani, Destia Aulia, Annisa Syafa Azura azura Azzahra, Larasabella Bagus Muhammad Ihsan Benedikta Anjelica Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprilia Dinasti Aprillia Djohan, Herlinda Edi Santoso Emilda Sari Fadilla, Alda Nur Fahma Ajeng Al-qorifah Fahrani, Chantika Lailatussa'adah Fitra Fathuhudin Grovindo Grovindo Hartati, Fitri Ulya Herlinda Djohan Imma Fatayati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Jenny Ratnawati, Gervacia Jessica Ningtyas Berlianti Jordan, Aristo Juliana Cristyaningsih Kartini Kartini Laila Kamilla Laila Kamilla Linda Triana Linda Triana Linda Triana Liza Harum Nur Annisa Maretalinia Maretalinia Maretalinia, Maretalinia Maulidiyah Salim Merda Sri Rahayu Monalita, Ria Muhammad Ihsan, Bagus Munardi, Fhatia Natasya Natasya Intan Ramadhani Nurhayati, Etiek Nurul Uq'ma P Purwanto Panyada Cholsakhon Perdede, Lusi Hernawati Rahayu, Merda Sri Ramadan, Aditiya Ratnawaty, Gervacia Jenny Reza Setiawan Reza, Nur Rezky, Muhammad Andyka Riska Alfianita Rizki Zanuba Arifah Romadi Romadi Sadid, Randy Samwidya, Veronika Sriayu Sharen Sen Slamet Sri Tumpuk Sugito Sugito Sugito Sugito Supriyanto Supriyanto Supriyanto Supriyanto Sutriswanto Sutriswanto Suwandi, Edy Syahdilla, Ade Syopingi Syopingi Syopingi, Syopingi Tantri Rahmatika Tarisya Fitri Pratiwi Theresia Indah Budhy Sulisetyawati Tika, Dian Tumpuk, Sri Veny Veny Wahdaniah Wahyudi Wicaksono, Didik Yunus, Mohd