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All Journal Jurnal Kesehatan: Jurnal Ilmu- Ilmu Keperawatan, Kebidanan, Farmasi dan Analis Kesehatan INDOGENIUS
Ramdan, Siti Rahmah Kurnia
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Health Professions Medicine & Pharmacology Nursing Public Health

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Determination of Anthocyanin Levels in Telang Flower (Clitoria Ternatae) Using the Differential pH Method Based on Three Types of Solvents Ramdan, Siti Rahmah Kurnia; Wulandari, Risda
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 10 No. 1 (2023): Jurnal Kesehatan (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v10i1.203

Abstract

Anthocyanins are pigments that are soluble in polar solvents naturally and belong to the flavonoid group found in various types of plants. As the name implies, this pigment gives color to the flowers, fruit, and leaves, and has been widely used as a natural colorant in various food products and other applications. Telang flower is an example of a plant that has high levels of anthocyanins. The research method to prepare the test sample used was extraction by maceration using 3 types of solvents ethanol 96%, n-butanol and ethyl acetate. The test was carried out using the differential pH method using UV-Vis spectrophotometry at 510 nm and 700 nm. The assay results obtained were the average anthocyanin content of telang flower extract in ethanol 96% 0,946%, n-butanol 1,684%, ethyl acetate 0,181%. The results of data analysis showed that all the average levels of telang flower extract from each solvent were different, in other words the average levels of telang flower extract from each solvent were significant differences. The highest anthocyanin levels in this study were produced in the telang flower extract with n-butanol as a solvent of 1,684% and the lowest in the telang flower extract with ethyl acetate solvent of 0,181%.
Utilization of Purple Sweet Potato Juice As an Alternative Violet Crystal in Coloring Streptococcus sp Bacteria and Escherichia coli Ruhimat, Undang; Mutmainah, Rahayu Nirmatul; Ramdan, Siti Rahmah Kurnia; Firdaus, Nabil Ridla
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 11 No. 2 (2024): Jurnal Kesehatan (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v11i2.769

Abstract

Gram staining is a staining method used to distinguish bacterial species into two major groups, namely Gram-positive and Gram-negative, Crystal violet is the primary (main) dye that will colour the target microorganisms. Staining in bacteria can be replaced using alternative substances with low cost, readily available and harmless, one of which uses substances that have anthocyanin content, namely purple sweet potato. The purpose of this study was to determine the results of using purple sweet potato as an alternative substance to replace crystal violet in staining Streptococcus sp and Escherichia coli bacteria. The method used is experimental. Based on the results of research that has been done on the use of purple sweet potato juice as an alternative substance to crystal violet in gram staining of Streptococcus sp and Escherichia coli bacteria, it shows that purple sweet potato material can be used at pH 6.5 and 5.7 while at pH 5.5 and 5 the bacteria are not perfectly coloured. So it can be concluded that purple sweet potato juice can be used as an alternative substance to Crystal violet in gram staining.
Study of Taurine Content in Energy Drink Powders: UV-Vis Spectrophotometric Method Ramdan, Siti Rahmah Kurnia; Athollah, Rifqi Nabil; Yusuf, Anna L; Nugraha, Davit; Wahlanto, Panji
INDOGENIUS Vol 4 No 2 (2025): INDOGENIUS
Publisher : Department of Publication of Inspirasi Elburhani Foundation Desa. Pamokolan, Kecamatan Cihaurbeuti, Kabupaten Ciamis, Provinsi Jawa Barat, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/igj.v4i2.593

Abstract

Background and Objective: Energy drinks are commonly consumed as a fast and affordable solution to fatigue, especially among adolescents and young adults. However, public knowledge about their appropriate use and the health risks of excessive intake remains limited. Taurine (2-aminoethanesulfonic acid), a conditionally essential amino acid found in many energy drinks, contributes to several physiological functions. Excessive intake of taurine may adversely affect kidney function. Method: The method of this study using UV-Vis spectrophotometry. A 0.1% ninhydrin solution was used for qualitative testing, while UV-Vis spectrophotometry was employed for quantitative measurement. Proteins were precipitated using Carrez I and Carrez II reagents before analysis. Results: A purplish-blue color change in the qualitative test confirmed the presence of taurine. Quantitative analysis revealed taurine concentrations of 0.025% and 0.012% in the two samples.  Conclusion: The results validate UV-Vis spectrophotometry as a practical and dependable technique for analyzing taurine, highlighting the importance of educating the public on the associated health risks of excessive consumption.
Co-Authors Anna L Yusuf Athollah, Rifqi Nabil Davit Nugraha Firdaus, Nabil Ridla Mutmainah, Rahayu Nirmatul panji wahlanto Undang Ruhimat Wulandari, Risda