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All Journal Jurnal Kesehatan: Jurnal Ilmu- Ilmu Keperawatan, Kebidanan, Farmasi dan Analis Kesehatan INDOGENIUS Pharmacy Genius Mukhtabar: Journal of Medical Laboratory Technology
Ramdan, Siti Rahmah Kurnia
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Determination of Anthocyanin Levels in Telang Flower (Clitoria Ternatae) Using the Differential pH Method Based on Three Types of Solvents Ramdan, Siti Rahmah Kurnia; Wulandari, Risda
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 10 No. 1 (2023): Jurnal Kesehatan (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v10i1.203

Abstract

Anthocyanins are pigments that are soluble in polar solvents naturally and belong to the flavonoid group found in various types of plants. As the name implies, this pigment gives color to the flowers, fruit, and leaves, and has been widely used as a natural colorant in various food products and other applications. Telang flower is an example of a plant that has high levels of anthocyanins. The research method to prepare the test sample used was extraction by maceration using 3 types of solvents ethanol 96%, n-butanol and ethyl acetate. The test was carried out using the differential pH method using UV-Vis spectrophotometry at 510 nm and 700 nm. The assay results obtained were the average anthocyanin content of telang flower extract in ethanol 96% 0,946%, n-butanol 1,684%, ethyl acetate 0,181%. The results of data analysis showed that all the average levels of telang flower extract from each solvent were different, in other words the average levels of telang flower extract from each solvent were significant differences. The highest anthocyanin levels in this study were produced in the telang flower extract with n-butanol as a solvent of 1,684% and the lowest in the telang flower extract with ethyl acetate solvent of 0,181%.
Study of Taurine Content in Energy Drink Powders: UV-Vis Spectrophotometric Method Ramdan, Siti Rahmah Kurnia; Athollah, Rifqi Nabil; Yusuf, Anna L; Nugraha, Davit; Wahlanto, Panji
INDOGENIUS Vol 4 No 2 (2025): INDOGENIUS
Publisher : Department of Publication of Inspirasi Elburhani Foundation Desa. Pamokolan, Kecamatan Cihaurbeuti, Kabupaten Ciamis, Provinsi Jawa Barat, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/igj.v4i2.593

Abstract

Background and Objective: Energy drinks are commonly consumed as a fast and affordable solution to fatigue, especially among adolescents and young adults. However, public knowledge about their appropriate use and the health risks of excessive intake remains limited. Taurine (2-aminoethanesulfonic acid), a conditionally essential amino acid found in many energy drinks, contributes to several physiological functions. Excessive intake of taurine may adversely affect kidney function. Method: The method of this study using UV-Vis spectrophotometry. A 0.1% ninhydrin solution was used for qualitative testing, while UV-Vis spectrophotometry was employed for quantitative measurement. Proteins were precipitated using Carrez I and Carrez II reagents before analysis. Results: A purplish-blue color change in the qualitative test confirmed the presence of taurine. Quantitative analysis revealed taurine concentrations of 0.025% and 0.012% in the two samples.  Conclusion: The results validate UV-Vis spectrophotometry as a practical and dependable technique for analyzing taurine, highlighting the importance of educating the public on the associated health risks of excessive consumption.
Penetapan Kadar Antosianin Ekstrak Etanol Bunga Telang (Clitoria ternatea) dengan Metode Differensial pH Ramdan, Siti Rahmah Kurnia; Alviansyah, Aldi
Pharmacogenius Journal Vol 3 No 1 (2024): Pharmacy Genius
Publisher : Yayasan Inspirasi El Burhani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/pharmgen.v3i01.306

Abstract

Pendahuluan: Antosianin merupakan keluarga besar polifenol yang menunjukkan pigmen  pada berbagai jenis  buah-buahan dan tanaman di alam. Pigmen yang ditunjukkan yaitu merah, merah jambu, biru dan ungu. Antosianin bertanggung jawab terhadap adanya warna biru dari bunga telang. Berbagai manfaat yang dimiliki antosianin bagi kesehatan diantaranya sebagai proteksi DNA, antiinflamasi, anti kanker, antioksidan, antidiabetes, mencegah penyakit kardiovaskular dan penyakit neurodegeneratif. Tujuan: Penelitian ini bertujuan untuk menentukan kadar antosianin pada ekstrak etanol bunga telang. Metode: Penentuan kadar antosianin pada ekstrak etanol  bunga telang dilakukan dengan metode differensial pH dengan menggunakan spektrofotometri uv-vis pada panjang gelombang 510 nm dan 700 nm. Hasil: Kadar antosianin pada ekstrak etanol bunga telang yang dilakukan pada penelitian ini dengan metode differensial pH diperoleh sebesar 0,853%. Kesimpulan: Dari hasil penelitian dapat disimpulkan bahwa kadar antosianin bunga telang dari hasil ekstraksi maserasi dengan  pelarut etanol 70% cukup tinggi dan berpotensi sebagai salah satu sumber obat bahan alam dan sebagai pewarna alami.
Uji Aktivitas Antioksidan Seduhan Bunga Telang (Clitoria ternatea L) Dengan Metode DPPH Ramdan, Siti Rahmah Kurnia
Pharmacogenius Journal Vol 3 No 1 (2024): Pharmacy Genius
Publisher : Yayasan Inspirasi El Burhani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/pharmgen.v3i01.328

Abstract

ABSTRAK   Pendahuluan: Bunga telang (Clitoria ternatea L.) merupakan tanaman yang memiliki pigmen ungu yang mencolok. Warna ungu dari tanaman ini disebabkan karena adanya kandungan antosianin yang dikenal aktivitas antioksidaanya yang sangat baik.  Minuman sari bunga telang dapat dibuat dengan cara penyeduhan pada suhu tertentu. Aktivitas antioksidan dari dekokta bunga telang dapat diketahui dengan berbagai metode diantaranya menggunakan  metode DPPH (2.2 diphenyl-1-picrylhydrazil). Tujuan: Tujuan dari penelitian ini untuk mengetahui aktivitas antioksidan seduhan bunga telang dengan aquades pada suhu 50oC dengan menggunakan metode DPPH. Metode: Penelitian ini dilakukan dengan membuat dekokta bunga telang pada suhu 50oC dengan mengukur daya hambatnya terhadap senyawa DPPH sebagai radikal bebas yang ditunjukkan oleh nilai absorbansi dari hasil rekasi dibandingkan dengan larutan kontrol dengan menggunakan Spektrofotometri UV-Vis pada panjang gelombang 400-800 nm. Hasil: Hasil penelitian ini berupa nilai absorbansi dan persen penghambatan dekokta bunga telang terhadap senyawa DPPH, aktivitas antioksidan ditunjukkan oleh nilai IC50 yaitu konsentrasi yang yang mampu menghambat 50% radikal bebas.  Dari hasil penelitian diperoleh bahwa aktivitas antioksidan dekokta bunga telang pada suhu 50oC memiliki aktivitas sedang. Kesimpulan: Dari data penelitian dapat disimpulkan bahwa dekokta bunga telang memiliki aktivitas antioksidan sedang yang sangat baik untuk dimanfaatkan sebagai minuman kesehatan.
Analysis of Sodium Benzoate Contents in Beverages Non-BPOM Packaged Fruit Juice Using UV-Vis Spectrophotometry Method Ramdan, Siti Rahmah Kurnia; Amalia, Syifa
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 2 (2025): Mukhtabar: Journal of Medical Laboratory Technology (October 2025)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i2.932

Abstract

Background & Objective: The rapid growth of the beverage industry in Indonesia has led to an increasing circulation of various soft drink products in society, including packaged fruit juice beverages produced by Micro, Small, and Medium Enterprises (MSMEs). These products often contain additional ingredients, particularly preservatives such as sodium benzoate. However, some manufacturers do not provide clear information regarding the concentration of these additives on product labels. The uncontrolled and unmonitored use of food additives may pose potential health risks, especially if the preservatives exhibit carcinogenic properties. This study aims to analyze the levels of sodium benzoate in packaged fruit juice drinks available on the market. The absence of sodium benzoate in the tested samples would indicate that the products are free from synthetic preservatives and therefore safe for consumption. Method: This study employed a descriptive-analytical design to determine the concentration of sodium benzoate in non-BPOM registered packaged fruit juice beverages. Quantitative analysis of sodium benzoate was performed using UV-Vis spectrophotometry. The population consisted of non-BPOM packaged fruit juice beverages available in local markets and retail outlets. Samples were collected using purposive sampling, based on product availability and labeling that did not specify preservative content. Result: The qualitative analysis using 5% FeCl3 reagent indicated positive results for the presence of sodium benzoate in both sample A and sample B. Furthermore, quantitative analysis revealed that the sodium benzoate concentrations in sample A and sample B were each 40 mg/kg (0.004%).   Conclusion: The findings of this study confirmed the presence of sodium benzoate in both non-BPOM packaged fruit juice samples. These levels were relatively low and remained within acceptable safety limits established by international food safety standards, indicating that the analyzed products are safe for consumption with respect to sodium benzoate content.
Co-Authors Alviansyah, Aldi Amalia, Syifa Anna L Yusuf Athollah, Rifqi Nabil Davit Nugraha panji wahlanto Wulandari, Risda