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All Journal Jurnal Kesehatan: Jurnal Ilmu- Ilmu Keperawatan, Kebidanan, Farmasi dan Analis Kesehatan
Mutmainah, Rahayu Nirmatul
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Health Professions Medicine & Pharmacology Nursing Public Health

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Almond as Alternative Media for Growth of Staphylococcus aureus and Escherichia coli Nurmalasari, Ary; Marlina, Leni; Ruhimat, Undang; Mutmainah, Rahayu Nirmatul
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 9 No. 2 (2022): Jurnal Kesehatan (Oktober 2022)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v9i2.344

Abstract

The high cost of media for microbial culture encourages researchers to create alternative media using local raw materials at low prices. One of the most important media compositions for bacterial growth is protein. This content can be obtained from nuts, one of which is almonds (Prunus dulcis). The purpose of this study   is   to  find  natural   media  that    has   the   potential    for   the    growth of microorganisms at a low price. The  research method  used is an experimental method to determine the growth of Escherichia coli and Staphylococcus aureus bacteria on natural media of almonds. The results showed the growth of Escherichia coli and Staphylococcus aureus bacteria in this alternative media of almonds. Based on the results of the study, it can be concluded that  almond  nut  media  can  be  applied  as  an  alternative  medium in   laboratory research, especially in the field of_microbiology. It is suggested that further research should be carried out on natural media which are sources of carbohydrates and proteins as alternative growth media for bacteria.
Utilization of Purple Sweet Potato Juice As an Alternative Violet Crystal in Coloring Streptococcus sp Bacteria and Escherichia coli Ruhimat, Undang; Mutmainah, Rahayu Nirmatul; Ramdan, Siti Rahmah Kurnia; Firdaus, Nabil Ridla
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 11 No. 2 (2024): Jurnal Kesehatan (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v11i2.769

Abstract

Gram staining is a staining method used to distinguish bacterial species into two major groups, namely Gram-positive and Gram-negative, Crystal violet is the primary (main) dye that will colour the target microorganisms. Staining in bacteria can be replaced using alternative substances with low cost, readily available and harmless, one of which uses substances that have anthocyanin content, namely purple sweet potato. The purpose of this study was to determine the results of using purple sweet potato as an alternative substance to replace crystal violet in staining Streptococcus sp and Escherichia coli bacteria. The method used is experimental. Based on the results of research that has been done on the use of purple sweet potato juice as an alternative substance to crystal violet in gram staining of Streptococcus sp and Escherichia coli bacteria, it shows that purple sweet potato material can be used at pH 6.5 and 5.7 while at pH 5.5 and 5 the bacteria are not perfectly coloured. So it can be concluded that purple sweet potato juice can be used as an alternative substance to Crystal violet in gram staining.
Co-Authors Firdaus, Nabil Ridla Nurmalasari, Ary Ramdan, Siti Rahmah Kurnia Undang Ruhimat