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All Journal Jurnal Kesehatan: Jurnal Ilmu- Ilmu Keperawatan, Kebidanan, Farmasi dan Analis Kesehatan Mukhtabar: Journal of Medical Laboratory Technology
Mutmainah, Rahayu Nirmatul
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Almond as Alternative Media for Growth of Staphylococcus aureus and Escherichia coli Nurmalasari, Ary; Marlina, Leni; Ruhimat, Undang; Mutmainah, Rahayu Nirmatul
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 9 No. 2 (2022): Jurnal Kesehatan (Oktober 2022)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v9i2.344

Abstract

The high cost of media for microbial culture encourages researchers to create alternative media using local raw materials at low prices. One of the most important media compositions for bacterial growth is protein. This content can be obtained from nuts, one of which is almonds (Prunus dulcis). The purpose of this study   is   to  find  natural   media  that    has   the   potential    for   the    growth of microorganisms at a low price. The  research method  used is an experimental method to determine the growth of Escherichia coli and Staphylococcus aureus bacteria on natural media of almonds. The results showed the growth of Escherichia coli and Staphylococcus aureus bacteria in this alternative media of almonds. Based on the results of the study, it can be concluded that  almond  nut  media  can  be  applied  as  an  alternative  medium in   laboratory research, especially in the field of_microbiology. It is suggested that further research should be carried out on natural media which are sources of carbohydrates and proteins as alternative growth media for bacteria.
Utilization of Purple Sweet Potato Juice As an Alternative Violet Crystal in Coloring Streptococcus sp Bacteria and Escherichia coli Ruhimat, Undang; Mutmainah, Rahayu Nirmatul; Ramdan, Siti Rahmah Kurnia; Firdaus, Nabil Ridla
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 11 No. 2 (2024): Jurnal Kesehatan (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v11i2.769

Abstract

Gram staining is a staining method used to distinguish bacterial species into two major groups, namely Gram-positive and Gram-negative, Crystal violet is the primary (main) dye that will colour the target microorganisms. Staining in bacteria can be replaced using alternative substances with low cost, readily available and harmless, one of which uses substances that have anthocyanin content, namely purple sweet potato. The purpose of this study was to determine the results of using purple sweet potato as an alternative substance to replace crystal violet in staining Streptococcus sp and Escherichia coli bacteria. The method used is experimental. Based on the results of research that has been done on the use of purple sweet potato juice as an alternative substance to crystal violet in gram staining of Streptococcus sp and Escherichia coli bacteria, it shows that purple sweet potato material can be used at pH 6.5 and 5.7 while at pH 5.5 and 5 the bacteria are not perfectly coloured. So it can be concluded that purple sweet potato juice can be used as an alternative substance to Crystal violet in gram staining.
Overview of Routine Blood Test Results in Rheumatoid Arthritis Patients in Ciamis Regency Mutmainah, Rahayu Nirmatul; Rabbani, Muhammad Abdul Hadi; Hanif, Rizal
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 2 (2025): Mukhtabar: Journal of Medical Laboratory Technology (October 2025)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i2.787

Abstract

Background & Objectives: Based on data from the Ciamis District Health Service Profile (2022), rheumatoid arthritis (RA) ranks 6th out of the 10 most common diseases at community health centers, with 16,195 cases. Systemic inflammation in RA can affect blood cell productivity. This has the potential to cause blood abnormalities, which can lead to diseases such as anemia, leukemia, or blood clotting problems. Routine blood tests are performed as a screening test to determine blood values in RA patients. The purpose of this study was to determine the results of routine blood tests in RA patients in Ciamis Regency. Method: This study used a descriptive method with total sampling technique. The examination was conducted on RA patients in Ciamis Regency in May 2024 with a sample size of 20 respondents. The study used secondary data that was processed into diagrams and then explained narratively. The study used a hematology analyzer located in the hematology laboratory of Ciamis Regional General Hospital. Results: This study found that the percentage of normal blood values for erythrocyte parameters was 70%, hemoglobin was 70%, hematocrit was 70%, average erythrocyte index was normal at 90%, leukocytes were 95%, average leukocyte types were normal at 80%, and thrombocytes were 90%. Low values were found in the erythrocyte (30%), hemoglobin (30%), hematocrit (30%), MCV (25%), MCH (10%), RDW SD (10%), and lymphocyte (11%) parameters. Meanwhile, high values were found in the parameters of white blood cells (10%), lymphocytes (8%), monocytes (15%), neutrophils (10%), eosinophils (40%), basophils (5%), and platelets (10%).     Conclusion: It can be concluded that the values for erythrocytes, hemoglobin, hematocrit, erythrocyte indices, leukocytes, leukocyte types, and platelets were generally within the normal range.
Co-Authors Firdaus, Nabil Ridla Hanif, Rizal Nurmalasari, Ary Rabbani, Muhammad Abdul Hadi Ramdan, Siti Rahmah Kurnia Syamsiar, Syamsiar Undang Ruhimat