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All Journal Wahana Peternakan Jurnal Ilmiah Ilmu-Ilmu Peternakan
Chanafi, Mochammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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OPTIMASI SUHU ANNEALING PROSES PCR AMPLIFIKASI GEN ATP1 A1 SAPI JABRES DAN GALEKAN: Optimization of Annealing Temperature PCR Process ATP1 A1 Gene Amplification in Jabres and Galekan Cows Dewi Khosiya Robba; Tuwi Ramsiati, Dyah; Indah Wulansari, Wahyuni; Chanafi, Mochammad; Asepriyadi; Ariyanti, Rina; Nihaya, Ullin
Wahana Peternakan Vol. 8 No. 1 (2024): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v8i1.1160

Abstract

Adanya kenaikan suhu pada lingkungan sekitar ternak sapi lokal Jabres dan sapi Galekan memiliki efek negatif terhadap perkembangan, pertumbuhan, produktivitas dan reproduksi-nya. Untuk itu, diperlukan metode untuk mengetahui kemampuan adaptasi sapi terhadap cekaman panas. Pada penelitian ini, sampel yang digunakan adalah DNA sapi lokal Jabres dan sapi Galekan dengan menggunakan metode penelitian molekuler in-vitro seperti isolasi DNA, Amplifikasi gen ATP1 A1 dengan Optimasi PCR, elektroforesis dan hasil visualisasinya. Isolasi DNA pada 8 sampel darah sapi Jabres dan sapi Galekan menunjukkan hasil isolasi DNA yang tepat dan sesuai dengan ditandai munculnya pita DNA yang terang dan tebal. Sedangkan, suhu annealing pada proses optimasi PCR yang digunakan untuk menempelkan primer forward ATP1 A1 5'- TCC CCA AGC TAG TGA CCA AG -3' dan primer reverse ATP1 A1 5'- TCT GTG GCT CTG ATT CTC CC -3' adalah 57oC, 57,3oC, 58,1oC, 59,3oC, 60,7oC, 61,9oC, 62,7oC, 63oC. Hasil optimasi PCR dielektroforesis dengan 100 volt selama 35 menit kemudian gel agarose hasil elektroforesis divisualisasikan menggunakan UV transilluminator sehingga didapatkan hasil suhu annealing yang optimal pada suhu 57 oC.   Kata Kunci : Sapi, PCR, gen ATP1A1, Annealing
Evaluasi Keberhasilan Kebuntingan Pada Sapi Madura Melalui Metode Kawin Alam: Evaluation of the success of pregnancy in Madura cattle through the natural mating method Wulansari, Wahyuni Indah; Dwi Kusumawati, Enike; Khosiya Robba, Dewi; Chanafi, Mochammad; Ramsiati, Dyah Tuwi; Nugroho Krisnaningsih, Aju Tjatur; Ariyanti, Rina
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 26 No 2 (2023): November 2023
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiiip.v26i2.29200

Abstract

The aim of this research is to determine the success of pregnancy in Madurese cattle using natural mating methods. The material used in this research was 36 female Madurese cattle and 3 male Madurese cattle. The research method used was field research by examining pregnancy and observing whether there was recurrent heat in cows that had been mated. The variables used in this research are Non Return Rate (NRR), Conception Rate (CR) and Service per Conception (S/C). Analysis of the data obtained was analyzed descriptively and discussed and compared with the literature. The research results showed that the percentage in Non Return Rate (NRR) for group A was 66.667%; group B 58.333% and group C 33.333%. The percentage of Conception Rate (CR) in group A was 66.667%; group B 58.333% and group C 33.333%. The Service per Conception (S/C) figure for group A was 1.5; group B 1.7 and group C 3. The percentage of Non Return Rate (NRR) for groups A and B meets the normal value, namely 66.667%, while group C is below the normal value of 33.333%. The Conception Rate (CR) percentage for groups A and B still meets the normal value, namely 66.667%, while group C is below the normal value of 33.333%. From the research results, it can be concluded that overall the evaluation of pregnancy in Madurese cattle using the natural mating method at the Beef Cattle Research Workshop is still in accordance with existing standards, this is a common occurrence in Indonesia which is adapted to natural conditions, livestock distribution and  livestock management.
Co-Authors Ariyanti, Rina Asepriyadi Dewi Khosiya Robba Dwi Kusumawati, Enike Indah Wulansari, Wahyuni Khosiya Robba, Dewi Nihaya, Ullin Nugroho Krisnaningsih, Aju Tjatur Ramsiati, Dyah Tuwi Tuwi Ramsiati, Dyah Wulansari, Wahyuni Indah