Claim Missing Document
Check
Articles

Found 8 Documents
Search

OPTIMASI SUHU ANNEALING PROSES PCR AMPLIFIKASI GEN ATP1 A1 SAPI JABRES DAN GALEKAN: Optimization of Annealing Temperature PCR Process ATP1 A1 Gene Amplification in Jabres and Galekan Cows Dewi Khosiya Robba; Tuwi Ramsiati, Dyah; Indah Wulansari, Wahyuni; Chanafi, Mochammad; Asepriyadi; Ariyanti, Rina; Nihaya, Ullin
Wahana Peternakan Vol. 8 No. 1 (2024): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v8i1.1160

Abstract

Adanya kenaikan suhu pada lingkungan sekitar ternak sapi lokal Jabres dan sapi Galekan memiliki efek negatif terhadap perkembangan, pertumbuhan, produktivitas dan reproduksi-nya. Untuk itu, diperlukan metode untuk mengetahui kemampuan adaptasi sapi terhadap cekaman panas. Pada penelitian ini, sampel yang digunakan adalah DNA sapi lokal Jabres dan sapi Galekan dengan menggunakan metode penelitian molekuler in-vitro seperti isolasi DNA, Amplifikasi gen ATP1 A1 dengan Optimasi PCR, elektroforesis dan hasil visualisasinya. Isolasi DNA pada 8 sampel darah sapi Jabres dan sapi Galekan menunjukkan hasil isolasi DNA yang tepat dan sesuai dengan ditandai munculnya pita DNA yang terang dan tebal. Sedangkan, suhu annealing pada proses optimasi PCR yang digunakan untuk menempelkan primer forward ATP1 A1 5'- TCC CCA AGC TAG TGA CCA AG -3' dan primer reverse ATP1 A1 5'- TCT GTG GCT CTG ATT CTC CC -3' adalah 57oC, 57,3oC, 58,1oC, 59,3oC, 60,7oC, 61,9oC, 62,7oC, 63oC. Hasil optimasi PCR dielektroforesis dengan 100 volt selama 35 menit kemudian gel agarose hasil elektroforesis divisualisasikan menggunakan UV transilluminator sehingga didapatkan hasil suhu annealing yang optimal pada suhu 57 oC.   Kata Kunci : Sapi, PCR, gen ATP1A1, Annealing
Rancang Alat Reaktor Pirolisis pada Pembuatan Base Oil dari Limbah Plastik dengan Proses Pyrolisis Ariyanti, Rina; Anggraini, Sinar Perbawani Abrina; Ma’sum, Zuhdi
Prosiding SENTIKUIN (Seminar Nasional Teknologi Industri, Lingkungan dan Infrastruktur) Vol 6 (2023): PROSIDING SENTIKUIN
Publisher : Fakultas Teknik Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Base oil is a basic material used in the production of lubricating oil, the types of Base oil can be mineral and synthetic. Base oil obtained from the plastic waste processing process by pyrolysis method is a synthetic type. Today the need for lubricating oil continues to increase. With the abundance of raw material sources in the form of plastic waste, it is very potential to pre-design a Base Oil Plant with the pyrolysis method. The base oil plant is designed with a capacity of 35000 tons / year, operating time for 300 days / year and 24 hours / day. The base oil production process uses a pyrolysis reactor that operates at a temperature of 524 °C and a pressure of 1 atm. The process occurs in batches every 1 hour where in the pyrolysis reactor there is a breakdown of PE compounds (plastic raw materials) in the form of long C chains into short C chains (C3 to C30). The purpose of designing this tool is to determine the capacity (dimensions and size of the tool) that is suitable for use in the production process with a capacity of 35000 tons / year through the calculation of mass and energy balances so that the production process can take place efficiently. The result of the design of this pyrolysis reactor tool is a reactor tank with material in the form of carbon steel SA 201 grade A with dimensions of 20 ft diameter and 24 ft height equipped with a heating coil. Has a volume capacity of 1340 bbl which can process PE melt from melting tanks of 1075 bbl per hour or equivalent to 194,444 kg / day (mass balance calculation) with semi-continuous operating conditions / batch (once every 1 hour). From the calculation of the mass balance, it is found that the efficiency of the products produced after going through advanced processes in the form of hydroprocessing and distillation is 86.79%. ABSTRAK Base oil merupakan bahan dasar yang dipakai dalam produksi minyak pelumas, Jenis-jenis dari Base oil dapat berupa mineral dan sintetik. Base oil yang didapatkan dari proses pengolahan limbah plastik dengan metode pirolisis merupakan jenis sintetik. Saat ini kebutuhan akan minyak pelumas terus meningkat. Dengan melimpahnya sumber bahan baku yang berupa limbah plastik maka sangat potensial untuk dilakukan Pra Rancang Pabrik Base Oil dengan metode pirolisis. Pabrik base oil dirancang dengan kapasitas 35000ton/tahun, waktu operasi selama 300 hari/tahun dan 24 jam/hari. Proses produksi base oil menggunakan alat reaktor pirolisis yang beroperasi pada suhu 524 °C dan tekanan 1 atm. Proses terjadi secara batch tiap 1 jam dimana pada reactor pirolisis terjadi pemecahan senyawa-senyawa PE (bahan baku plastik) yang berupa rantai C panjang menjadi rantai C pendek (C3 sampai dengan C30). Tujuan dari perancangan alat ini adalah untuk menentukan kapasitas (dimensi dan ukuran alat) yang sesuai untuk dipakai dalam proses produksi kapasitas 35000 ton/tahun melalui perhitungan neraca massa dan energi sehingga proses produksi dapat berlangsung dengan efisien. Hasil dari perancangan Alat Reaktor pirolisis ini adalah tanki reactor dengan bahan berupa carbon steel SA 201 grade A berdimensi ukuran diameter 20 ft dan tinggi 24 ft yang dilengkapi dengan coil pemanas. Mempunyai kapasitas volume sebesar 1340 bbl yang dapat memproses lelehan PE dari tanki melting sebesar 1075 bbl tiap jam atau setara 194.444 kg/hari (perhitungan neraca massa) dengan kondisi operasi secara semi kontinyu/batch (1 jam sekali). Dari perhitungan neraca massa didapatkan efisiensi produk yang dihasilkan setelah melalui proses lanjutan berupa hidroprocessing dan distilasi adalah sebesar 86,79%
Studi Amofer T. Harzianum terhadap Kandungan Protein Kasar, Serat Kasar, dan Lignin pada Jerami Serai Wangi (C. nardus L.) Ariyanti, Rina; Pamungkas, Dicky; Solehudin; Mubarak, Ade Syahrul
Prosiding SENTIKUIN (Seminar Nasional Teknologi Industri, Lingkungan dan Infrastruktur) Vol 6 (2023): PROSIDING SENTIKUIN
Publisher : Fakultas Teknik Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Citronella straw (Cymbopogon nardus) is waste from the processing of citronella essential oil which can reach 99% of the total raw materials of citronella. This straw waste still has the potential to be used as animal feed, especially ruminants because it still has a better protein content than rice straw (3-4%), which is around 5-6%. Judging from its nutritional content, citronella straw still has a fairly high fiber content of around 30-35% so it is necessary to do pretreatment in the form of ammoniation and fermentation before being used for animal feed as forage. The purpose of this research activity is to determine the effectiveness of ammonia and fermentation treatment and amofer (fermentation ammonia) to reduce crude fiber content while increasing nutrient levels, especially crude protein from citronella straw. The method used in the research activity was a Complete Randomized Design (RAL) with 4 treatments, namely P1 (control / no treatment), P2 (21 days ammoniation), P3 (8 days fermentation) and P4 (amofer). The parameters observed are the content of crude fiber, lignin as well as crude protein. The results obtained from research activities are on the parameters of crude fiber, between ammoniation and fermentation treatment is not significantly different, but significantly different from control and amofer treatment. Meanwhile, in lignin parameters and crude protein levels, ammoniation, fermentation and amofer treatments gave significantly different results to control and inter-treatment treatments. A significant decrease in crude fiber and lignin levels occurred in ammoniation, fermentation and amofer treatment of the control with the largest difference in reduction occurring in the amofer treatment, which was 6.61% in crude fiber parameters and 9.49% in lignin parameters. Crude protein experienced a significant increase in ammoniation, fermentation, and amofer treatment to the control treatment, with the highest percentage increase of 5.95% in the amofer treatment. Ammonia and fermentation treatment by Trichoderma harzianum is effective for reducing crude fiber and lignin levels and increasing crude protein levels in citronella straw with the highest effectiveness occurring in amofer treatment. Abstrak Jerami serai wangi (Cymbopogon nardus L) merupakan limbah sisa hasil dari pengolahan minyak atsiri serai wangi (citronella) yang jumlahnya dapat mencapai 99% dari total bahan baku serai wangi. Limbah berupa jerami ini masih berpotensi untuk dimanfaatkan sebagai pakan ternak khususnya ternak ruminansia karena masih mempunyai kandungan protein yang lebih baik dari jerami padi (3-4%) yaitu sekitar 5-6%. Dilihat dari kandungan nutrisinya, jerami serai wangi masih mempunyai kadar serat yang cukup tinggi yaitu sekitar 30 – 35% sehingga perlu dilakukan pretreatment berupa amoniasi dan fermentasi sebelum digunakan untuk pakan ternak sebagai hijauan. Tujuan dari kegiatan penelitian ini adalah untuk mengetahui efektivitas dari perlakuan amoniasi dan fermentasi serta amofer (amoniasi fermentasi) untuk menurunkan kadar serat kasar sekaligus menaikkan kadar nutrient terutama protein kasar dari jerami serai wangi. Metode yang digunakan dalam kegiatan penelitian adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan yaitu P1 (kontrol/tanpa perlakuan), P2 (amoniasi 21 hari), P3 (fermentasi 8 hari) dan P4 (amofer). Parameter yang diamati adalah kadar serat kasar, lignin serta protein kasar. Hasil yang didapatkan dari kegiatan penelitian yaitu pada parameter serat kasar, antara perlakuan amoniasi dan fermentasi tidak berbeda signifikan, akan tetapi berbeda signifikan terhadap perlakuan kontrol dan amofer. Sedangkan pada parameter lignin dan kadar protein kasar, perlakuan amoniasi, fermentasi maupun amofer memberikan hasil berbeda signifikan terhadap perlakuan kontrol maupun antar perlakuan. Penurunan kadar serat kasar dan lignin secara signifikan terjadi pada perlakuan amoniasi, fermentasi dan amofer terhadap kontrol dengan selisih penurunan terbesar terjadi pada perlakuan amofer yaitu sebesar 6,61% pada parameter serat kasar dan sebesar 9,49% pada parameter lignin. Protein kasar mengalami kenaikan yang signifikan pada perlakuan amoniasi, fermentasi, maupun amofer terhadap perlakuan kontrol, dengan persentase kenaikan tertinggi sebesar 5,95% pada perlakuan amofer. Perlakuan amoniasi dan fermentasi oleh jamur Trichoderma harzianum efektif untuk menurunkan kadar serat kasar dan lignin serta menaikkan kadar protein kasar pada jerami serai wangi dengan efektifitas tertinggi terjadi pada perlakuan amofer.
STOCK POWER: Uncovering the Effect of Asset Structure, Sales Growth, and Business Risk on Share Prices with the Support of Capital Structure as a Regulator Ariyanti, Rina
KINERJA: Jurnal Manajemen Organisasi dan Industri Vol. 2 No. 2 (2023): KINERJA: Jurnal Manajemen Organisasi dan Industri
Publisher : Sekolah Menengah Kejuruan (SMK) Pustek

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37481/jmoi.v2i2.108

Abstract

This study aims to determine the effect of Asset Structure, Sales Growth and Business Risk on Stock Prices with Capital Structure as a moderating variable. This type of research is quantitative associative. The sampling method with the "purposive sampling" method is to select samples with certain considerations. The company's sample consists of 29 companies listed on the LQ 45 index. Data processing techniques using the E-Views Version 10.0 program use descriptive statistics, two-model tests, model tests, classical assumption tests, hypothesis tests, and determination coefficient tests. The results showed that simultaneously Asset Structure, Sales Growth and Business Risk had a positive effect on Share Price. Capital Structure does not moderate the relationship between Asset Structure, Business Risk, Sales Growth and Share Price.
FACTORS INFLUENCING SECONDARY SCHOOL STUDENTS’ MOTIVATION TO LEARN ENGLISH IN EFL CLASSROOMS Ariyanti, Rina; Solihati, Tri Agustini; Julistiana, Risma
VOX EDUKASI: Jurnal Ilmiah Ilmu Pendidikan Vol 16, No 2 (2025): NOPEMBER
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/ve.v16i2.5359

Abstract

ABSTRAKPenelitian ini mengkaji faktor-faktor intrinsik dan ekstrinsik utama yang memengaruhi motivasi siswa sekolah menengah dalam belajar bahasa Inggris di kelas EFL di Indonesia. Meskipun bahasa Inggris memiliki peran global yang penting, banyak siswa mengalami kesulitan dalam motivasi. Penelitian ini bertujuan mengidentifikasi pengaruh-pengaruh motivasi tersebut dengan menggunakan pendekatan kualitatif deskriptif. Penelitian ini menggunakan metodologi penelitian kualitatif dengan desain survei. Data dikumpulkan dari 99 siswa melalui kuesioner skala Likert 20 item dan dari 6 siswa melalui wawancara mendalam. Analisis dilakukan dengan perhitungan persentase. Hasil penelitian menunjukkan bahwa faktor intrinsik seperti pengembangan diri, peningkatan bahasa, dan pemahaman budaya berkontribusi pada motivasi, meskipun kepercayaan diri siswa masih rendah. Faktor ekstrinsik seperti ambisi karier, tujuan pendidikan, dan harapan sosial juga berperan signifikan. Faktor-faktor ini saling berinteraksi, meningkatkan motivasi jika didukung, namun menurun jika tidak. Penelitian ini berimplikasi pada pentingnya guru meningkatkan motivasi siswa melalui penumbuhan minat, penguatan kepercayaan diri, serta pengaitan pembelajaran dengan tujuan dan nilai pribadi siswa.Kata Kunci: motivasi, faktor intrinsik, faktor ekstrinsik, pembelajaran bahasa Inggris, EFLABSTRACTThis study investigates key intrinsic and extrinsic factors affecting secondary school students' motivation to learn English in Indonesian EFL classrooms. Despite English's global importance, many students struggle with motivation. The research aims to identify these motivational influences using a descriptive qualitative approach. This study employed a qualitative research methodology with a survey design. Data were collected from 99 students via a 20-item Likert-scale questionnaire and from 6 students through in-depth interviews. Analysis involved percentage calculations. The results of the study indicate that intrinsic factors such as self-development, language improvement, and cultural understanding contribute to motivation, although students' confidence remains low. Extrinsic factors including career ambitions, educational goals, and social expectations also play a significant role. These factors interact, enhancing motivation when supported but declining otherwise. This study implies the importance of enhancing students’ motivation by fostering interest, strengthening self-confidence, and linking learning to their personal goals and values.Keywords: English as a foreign language (EFL) classroom, intrinsic, extrinsic, motivation
Evaluasi Keberhasilan Kebuntingan Pada Sapi Madura Melalui Metode Kawin Alam: Evaluation of the success of pregnancy in Madura cattle through the natural mating method Wulansari, Wahyuni Indah; Dwi Kusumawati, Enike; Khosiya Robba, Dewi; Chanafi, Mochammad; Ramsiati, Dyah Tuwi; Nugroho Krisnaningsih, Aju Tjatur; Ariyanti, Rina
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 26 No 2 (2023): November 2023
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiiip.v26i2.29200

Abstract

The aim of this research is to determine the success of pregnancy in Madurese cattle using natural mating methods. The material used in this research was 36 female Madurese cattle and 3 male Madurese cattle. The research method used was field research by examining pregnancy and observing whether there was recurrent heat in cows that had been mated. The variables used in this research are Non Return Rate (NRR), Conception Rate (CR) and Service per Conception (S/C). Analysis of the data obtained was analyzed descriptively and discussed and compared with the literature. The research results showed that the percentage in Non Return Rate (NRR) for group A was 66.667%; group B 58.333% and group C 33.333%. The percentage of Conception Rate (CR) in group A was 66.667%; group B 58.333% and group C 33.333%. The Service per Conception (S/C) figure for group A was 1.5; group B 1.7 and group C 3. The percentage of Non Return Rate (NRR) for groups A and B meets the normal value, namely 66.667%, while group C is below the normal value of 33.333%. The Conception Rate (CR) percentage for groups A and B still meets the normal value, namely 66.667%, while group C is below the normal value of 33.333%. From the research results, it can be concluded that overall the evaluation of pregnancy in Madurese cattle using the natural mating method at the Beef Cattle Research Workshop is still in accordance with existing standards, this is a common occurrence in Indonesia which is adapted to natural conditions, livestock distribution and  livestock management.
Mengungkap Keterkaitan Inklusi dan Literasi Keuangan dengan Kinerja dan Keberlanjutan UMKM di Perkotaan Ariyanti, Rina
AKADEMIK: Jurnal Mahasiswa Ekonomi & Bisnis Vol. 4 No. 1 (2024): AKADEMIK: Jurnal Mahasiswa Ekonomi & Bisnis
Publisher : Perhimpunan Sarjana Ekonomi dan Bisnis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37481/jmeb.v4i1.651

Abstract

Within the economic landscape of Depok City, the Micro, Small, and Medium Enterprises (MSMEs) sector holds a pivotal position, contributing significantly to the local economy. Despite its importance, a myriad of challenges confront MSMEs in the city, encompassing issues related to financial access and insufficient financial literacy. Consequently, this study seeks to empirically examine and elucidate the influence of financial inclusion and financial literacy, with business sustainability serving as an intermediary variable within the MSME sector of Depok City. Utilizing the Non-Probability sampling method, this research engaged MSME participants in Depok City, with a population of 7911, employing the Slovin formula to determine a representative sample size of 200. Employing the Partial Least Square- Structural Equation Modeling (PLS-SEM) method for data analysis, this quantitative research aims to contribute valuable insights to the existing body of knowledge. The findings reveal a discernible impact of Financial Inclusion on both the Performance and Sustainability of MSMEs. Similarly, Financial Literacy emerges as a significant factor influencing the Performance and Sustainability of MSMEs. Furthermore, Business Sustainability is identified as a mediating factor, demonstrating its capacity to mediate the relationship between Financial Inclusion and MSME Performance, as well as between Financial Literacy and MSME Performance. This study serves as a valuable reference for MSME stakeholders, underscoring the imperative nature of accessible banking services for optimal financial outcomes. The results advocate for enhanced financial literacy as a means to fortify the performance and sustainability of MSMEs. In conclusion, the study attests to the resilience of many MSMEs in Depok City, substantiating their ability to endure and thrive in their business endeavors, evident by a sustained operational existence exceeding five years.
Implementasi Nilai Sakai Sambayan Dalam Kehidupan Sosial Masyarakat Lampung Modern Ariyanti, Rina; Desiana Putri, Dilla
JURNAL MANAJEMEN PENDIDIKAN Vol 13, No 2 (2025): Jurnal Manajemen Pendidikan
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jmp.v13i2.12891

Abstract

IMPLEMENTATION OF SAKAI SAMBAYAN VALUES IN THE SOCIAL LIFE OF MODERN LAMPUNG SOCIETYSakai Sambayan is a noble cultural value of the people of Lampung that describes the spirit of mutual cooperation, togetherness, and social solidarity among others. This value is the moral and social foundation in various aspects of the life of the people of Lampung, both in customary, social, and religious activities. This study aims to describe how the values of Sakai Sambayan are applied in the life of the modern Lampung people, as well as identify the factors that support and hinder its implementation in the midst of rapid social change. The research method used is a descriptive qualitative approach with data collection techniques through observation, interviews, and documentation studies. The results of the study show that the value of Sakai Sambayan is still maintained and applied in various community activities, such as mutual cooperation in improving public facilities, the implementation of traditional ceremonies, religious activities, and social collaboration in the digital space through online fundraising and community-based social actions. Factors that support the preservation of these values include the strong internalization of local culture in daily life, the active role of traditional and religious leaders, and the support of families and educational institutions in instilling the value of togetherness. However, the application of Sakai Sambayan values faces a number of challenges, such as the increase in individualism due to modern lifestyles, the influence of urbanization that weakens social ties, and the declining participation of the younger generation in traditional social activities. Therefore, the preservation of Sakai Sambayan values needs to be done in an adaptive and innovative manner to remain relevant in the modern era through education, digital media, and community-based social activities.