Laboratorium biologi Sekolah Menengah Atas (SMA) harus dapat menjamin kesehatan dan keselamatan kerja (K3) selama peserta didik berada di laboratorium sehingga kompetensi pembelajaran dapat tercapai. K dapat terlaksana dengan adanya biosafety dan biosecurity. Fokus kajian dalam artikel ini untuk mengetahui kondisi laboratorium biologi SMA berdasarkan biosafety dan biosecurity. Penelitian ini menggunakan pendekatan deskriptif kuantitatif dan kualitatif. Sampel sekolah dipilih secara random sampling dari SMA negeri dan terakreditasi A di wilayah Kabupaten Sleman. Pengambilan data menggunakan tiga cara, yaitu instrumen angket, observasi dan wawancara dilakukan kepada kepala laboratorium biologi dan laboran. Hasil menunjukkan sekolah belum memenuhi standar sarana dan prasarana sesuai kriteria biosafety level 1 dan biosecurity, belum adanya penggunaan Alat Pelindung Diri (APD) oleh peserta didik untuk mendukung terlaksananya K3, dan kinerja belum sesuai implementasi biosafety dan biosecurity. Hal ini menunjukkan bahwa diperlukan peningkatan kualitas laboratorium biologi SMA untuk memenuhi kriteria biosafety (level 1) dan biosecurity. High school biology laboratories (SMA) must be able to guarantee Occupational Health and Safety (K3) while students are in the laboratory so that learning competencies can be achieved. K3 can be implemented with biosafety and biosecurity. The focus of the study in this article was to determine the condition of the high school biology laboratory based on biosafety and biosecurity. This research used  quantitative and qualitative descriptive approach. The sample schools were randomly selected from state high schools and were accredited A in Sleman Regency area. Data collection used three methods: questionnaire instruments, observations, and interviews with the head of the biology laboratory and laboratory assistant. The results showed that the school has not met the facilities and infrastructure standards according to the biosafety level 1 and biosecurity criteria, students have not used Personal Protective Equipment (PPE) to support the implementation of K3, and performance has not met the implementation of biosafety and biosecurity. This show that it is necessary to improve the quality of high school biology laboratories to meet biosafety criteria (level 1) and biosecurity.