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Deskripsi Manajemen Sumber Daya Manusia Laboratorium Biologi SMA di Kota Yogyakarta Safitri, Novita Ramadhani; Ikrima, Zalfa; Salsabilla, Farah; Insra, Atala Rania; Saifuddin, Much Fuad; Puspitasari, Etika Dyah
BIOEDUKASI (Jurnal Pendidikan Biologi) Vol 15, No 1 (2024): BIOEDUKASI, MEI 2024
Publisher : UNIVERSITAS MUHAMMADIYAH METRO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/bioedukasi.v15i1.5876

Laboratory management is an integral part of laboratory activities related to human resources and professional staff who manage skillfully in a combination of management and laboratory equipment. This study aims to describe the management of human resources in the biology laboratory of Senior High School in Yogyakarta City by looking at the achievement of its duties, which are adjusted to the Regulation of the Minister of National Education No. 26 of 2008 in supporting the laboratory management of School A, School B, and School C. This study used descriptive analysis with a qualitative approach. The data analysis technique in this research is using descriptive analysis of interview results. The research results from the three schools were insufficient to meet the standards of school laboratory personnel, because there was still a shortage of laboratory personnel that should be needed. The competencies listed in the Regulation of the Minister of National Education No. 26 of 2008 of the three schools have been implemented.

Biosafety pada Laboratorium Biologi Sekolah Menengah Atas Se-Kota Yogyakarta Sari, Maulida Ivana; Nisa, Rifda Khairun; Messy, Messy; Rahmawati, Senja Amalia; Saifuddin, Much. Fuad; Puspitasari, Etika Dyah
Bioeduca : Journal of Biology Education Vol 4, No 2 (2022): Bioeduca : Journal of Biology Education
Publisher : Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/bioeduca.v4i2.12366

Laboratorium biologi memiliki potensi besar menyebabkan kontaminasi atau menyebarkan penyakit, termasuk pada level laboratorium biologi SMA. Oleh karena itu, biosafety perlu dipastikan terimplementasi guna mengurangi risiko paparan agen yang menyebarkan penyakit. Penelitian ini untuk mengetahui penerapan Biosafety di Laboratorium Biologi SMA di kota Yogyakarta. Pengambilan sampel sekolah secara random dari sekolah yang ada di kota Yogyakarta. Pengambilan data melalui wawancara secara langsung terhadap laboran atau guru biologi sekolah A, B, dan C. Data dianalisis secara deskriptif kualitatif. Hasil analisis menunjukkan sekolah A, B, dan C telah menerapkan Biosafety saat praktikum di laboratorium biologi. Hanya saja ada beberapa hal dari ketiga sekolah tersebut yang harus diperbaiki dan ditingkatkan agar menjadi laboratorium biologi sekolah dengan keamanan yang baik.

Quality of biology laboratory infrastructure and Facilities in High School Arum, Sekar Ganda Ning Tyas; Saifuddin, Much Fuad; Pratiwi, Silfi; Isnayanti, Tri Rahayu; Rahma, Dinda Iftiqa; Puspitasari, Etika Dyah; Wulandari, Retno; Purbosari, Purwanti Pratiwi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i2.4459

Practicum is one of the essential learning activities for students psychomotor and fosters scientific attitudes in students. Therefore, adequate laboratory infrastructure is needed. Biology laboratory standards are contained in the regulation of the Minister of national education No. 24 of 2007 concerning Standards for Educational Facilities and Infrastructure. The research as descriptive qualitative was conducted at School A, School B, and School C with data collection techniques using observation and interviews. The study results indicate that laboratory facilities and infrastructure, including infrastructure, facilities and Biosecurity Level 1, follow the standards set in the regulation of the Minister of national education No. 24 of 2007. Based on the research that has been done, the most suitable facilities and infrastructure according to the standards of regulation of the Minister of national education No. 24 of 2007 are School B. Fulfillment of standards can support the smooth running of practicum. Besides, schools must keep raising standards to a higher level.

PENGELOLAAN LIMBAH LABORATORIUM BIOLOGI SMA DI KABUPATEN BANTUL, D.I. YOGYAKARTA Wulandari, Shely Dwi; Ghoida, Siti Najah; Pangastuti, Syifa; Ni'mah, Ulfatun; Basri, Frida Nora Ayu; Saifuddin, Much. Fuad; Puspitasari, Etika Dyah
Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi Vol 6, No 2 (2022): DIDAKTIKA BIOLOGI
Publisher : Universitas Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/dikbio.v6i2.4769

Limbah laboratorium biologi berasal dari percobaan atau praktikum dalam bentuk padat dan cair. Limbah padat pada laboratorium biologi biasanya berupa kertas atau endapan yang masih dengan mudah teratasi. Sedangkan limbah cair, biasanya tidak menggunakan bahan/reagen yang berbahaya. Tujuan dari penelitian ini untuk mengetahui pengelolaan limbah di laboratorium biologi SMA. Metode yang digunakan dalam penelitian adalah secara deskriptif dengan teknik pengumpulan data berupa dengan wawancara, observasi dan dokumentasi. Penelitian dilakukan pada tahun pelajaran 2021/2022 di SMA Negeri Kabupaten Bantul, D. I. Yogyakarta, yaitu SMA Negeri 1 Pleret, SMA Negeri 1 Jetis, dan SMA Negeri 1 Kasihan. Hasil wawancara dengan laboran sekolah dideskripsikan secara kualitatif dan dokumentasi dilakukan untuk mendukung data yang telah diperoleh, sedangkan hasil observasi dilakukan secara kuantitatif dalam persentase. Hasil penelitian menunjukkan pengelolaan limbah laboratorium biologi di ketiga sekolah dapat dikategorikan dengan sangat baik dengan total persentase penilaian tidak kurang dari 90%. Penilaian dalam pengelolaan limbah laboratorium biologi hanya pada pengelolaan limbah padat dan pengelolaan limbah cair saja dikarenakan ketiga sekolah tidak menggunakan bahan-bahan yang berbahaya (B3) dalam melaksanakan praktikum. Meskipun demikian, limbah yang dihasilkan dari praktikum tetap memerlukan pengelolaan khusus. Biological laboratory waste comes from experiments or practicum in solid and liquid form. Solid waste in a biology laboratory is usually paper or sludge, which is still easily resolved. Meanwhile, liquid waste usually does not use hazardous materials/reagents. This study aimed to determine waste management in high school biology laboratory.The method used in this research was descriptive with data collection techniques in the form of interview, observation and documentation. The research was conducted in the 2021/2022 school year at SMA Negeri Bantul Regency, Special Region of Yogyakarta, namely SMA Negeri 1 Pleret, SMA Negeri 1 Jetis, dan SMA Negeri 1 Kasihan. The results of interviews with school laboratory assistants were described qualitatively and documentation was carried out to support the data that had been obtained, while the results of observations were carried out quantitatively in percentages.The results showed that the management of biological laboratory waste in the three schools could be categorized very well with a total percentage of not less than 90%. Assessment in the management of biological laboratory waste was only on solid and liquid waste management because the three schools did not use hazardous materials in carrying out practicums. Even so, the waste generated from the practicum still requires special management.

ANALISIS BIOSAFETY DAN BIOSECURITY LABORATORIUM BIOLOGI SMA DI KABUPATEN SLEMAN Faisal, Muhammmad; Lestari, Diah Ayu; Loka, Fika Pita; Sausannuri, Isnairana; Permatasari, Indah; Fiodora, Alika; Saifuddin, Much. Fuad; Puspitasari, Etika Dyah
Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi Vol 7, No 1 (2023): DIDAKTIKA BIOLOGI
Publisher : Universitas Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/dikbio.v7i1.4767

Laboratorium biologi Sekolah Menengah Atas (SMA) harus dapat menjamin kesehatan dan keselamatan kerja (K3) selama peserta didik berada di laboratorium sehingga kompetensi pembelajaran dapat tercapai. K dapat terlaksana dengan adanya biosafety dan biosecurity. Fokus kajian dalam artikel ini untuk mengetahui kondisi laboratorium biologi SMA berdasarkan biosafety dan biosecurity. Penelitian ini menggunakan pendekatan deskriptif kuantitatif dan kualitatif. Sampel sekolah dipilih secara random sampling dari SMA negeri dan terakreditasi A di wilayah Kabupaten Sleman. Pengambilan data menggunakan tiga cara, yaitu instrumen angket, observasi dan wawancara dilakukan kepada kepala laboratorium biologi dan laboran. Hasil menunjukkan sekolah belum memenuhi standar sarana dan prasarana sesuai kriteria biosafety level 1 dan biosecurity, belum adanya penggunaan Alat Pelindung Diri (APD) oleh peserta didik untuk mendukung terlaksananya K3, dan kinerja belum sesuai implementasi biosafety dan biosecurity. Hal ini menunjukkan bahwa diperlukan peningkatan kualitas laboratorium biologi SMA untuk memenuhi kriteria biosafety (level 1) dan biosecurity. High school biology laboratories (SMA) must be able to guarantee Occupational Health and Safety (K3) while students are in the laboratory so that learning competencies can be achieved. K3 can be implemented with biosafety and biosecurity. The focus of the study in this article was to determine the condition of the high school biology laboratory based on biosafety and biosecurity. This research used quantitative and qualitative descriptive approach. The sample schools were randomly selected from state high schools and were accredited A in Sleman Regency area. Data collection used three methods: questionnaire instruments, observations, and interviews with the head of the biology laboratory and laboratory assistant. The results showed that the school has not met the facilities and infrastructure standards according to the biosafety level 1 and biosecurity criteria, students have not used Personal Protective Equipment (PPE) to support the implementation of K3, and performance has not met the implementation of biosafety and biosecurity. This show that it is necessary to improve the quality of high school biology laboratories to meet biosafety criteria (level 1) and biosecurity.

ACTIVITY OF NONI EXTRACT (Morinda citrifolia L.) AS A BIOFUNGICIDE AGAINST THE GROWTH OF THE FUNGUS Fusarium oxysporum IN VITRO Puspitasari, Etika Dyah; Matsjeh, Sabirin; Mardiah, Ainun; Juni Lestari, Tri Tunggal; Kumalasari, Dewi; Kartika Sari, Prita Anggraeni
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : LPPM Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v14i1.1185

Fusarium oxysporum is a fungus that attacks various plants and causes wilting. Several plants, including bananas, have experienced a decrease in productivity due to the Fusarium fungus. This research aims to determine the effect of the whole plant of noni plants (fruits, leaves, and twigs), methanol extract, and ethyl acetate on the growth of fusarium fungi. To determine the optimal extract concentration to inhibit fungal growth. This research is a factorial study with independent variables in the form of extract, ingredients (fruit, leaves, and twigs), and differences in concentration. The activity of the extract as a biofungicide was analyzed based on the fungal growth inhibition zone. The results were analyzed using Anava and continued with the Duncan test. The research results show that methanol extract and ethyl acetate extract of noni have the potential as bio fungicides for the fungus Fusarium oxysporum. Noni fruit ethyl acetate extract at a concentration of 80% is optimum for inhibiting the growth of Fusarium oxysporum

ACTIVITY OF NONI EXTRACT (Morinda citrifolia L.) AS A BIOFUNGICIDE AGAINST THE GROWTH OF THE FUNGUS Fusarium oxysporum IN VITRO Puspitasari, Etika Dyah; Matsjeh, Sabirin; Mardiah, Ainun; Juni Lestari, Tri Tunggal; Kumalasari, Dewi; Kartika Sari, Prita Anggraeni
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v14i1.1185

Fusarium oxysporum is a fungus that attacks various plants and causes wilting. Several plants, including bananas, have experienced a decrease in productivity due to the Fusarium fungus. This research aims to determine the effect of the whole plant of noni plants (fruits, leaves, and twigs), methanol extract, and ethyl acetate on the growth of fusarium fungi. To determine the optimal extract concentration to inhibit fungal growth. This research is a factorial study with independent variables in the form of extract, ingredients (fruit, leaves, and twigs), and differences in concentration. The activity of the extract as a biofungicide was analyzed based on the fungal growth inhibition zone. The results were analyzed using Anava and continued with the Duncan test. The research results show that methanol extract and ethyl acetate extract of noni have the potential as bio fungicides for the fungus Fusarium oxysporum. Noni fruit ethyl acetate extract at a concentration of 80% is optimum for inhibiting the growth of Fusarium oxysporum

Pengaruh pemberian variasi konsentrasi rootmost terhadap inisiasi akar tanaman mint (Mentha rotundifolia (L.) Huds.) Hayya, Nina Fadia; Puspitasari, Etika Dyah
Jurnal Esabi (Jurnal Edukasi dan Sains Biologi) Vol. 6 No. 2 (2024)
Publisher : Universitas Bung Hatta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37301/esabi.v6i2.80

Salah satu tanaman yang memiliki banyak peminat ialah tanaman mint. Sehingga diperlukan adanya pengembangan pembudidayaan tanaman mint di Indonesia. Selain karena jumlah peminat yang tinggi, hal ini juga agar dapat mengurangi ketergantungan impor bahan baku industri minyak mint. Salah satu tanaman mint yang mudah dibudidayakan ialah spesies Mentha rotudinfolia (L.) Huds. Pembudidayaan menggunakan metode stek pucuk dengan tambahan zat tumbuh Rootmost. Penelitian ini bertujuan untuk mengetahui konsentrasi rootmost yang paling efektif dalam mempengaruhi pertumbuhan akar tanaman mint. Penelitian ini merupakan penelitian eksperimen dengan memotong pucuk tanaman mint sepanjang 5 cm, lalu direndam dalam rootmost pada konsentrasi 0%, 0,1%, 0,2%, 0,3%, dan 0,4%. Kemudian stek ditanam ke dalam media cocopeat dalam tray selama 2 minggu. Parameter yang diukur adalah jumlah akar dan panjang akar. Hasil penelitian dianalisis menggunakan uji One Way Anova dan dilanjutkan dengan uji LSD dengan taraf signifikansi 0,5%. Hasil penelitian diperoleh yaitu konsentrasi rootmost yang paling efektif dalam pembentukan akar ada pada konsentrasi 0,1%, sedangkan yang paling efektif dalam pemanjangan akar ada pada konsentrasi 0,4%.

The characteristics teaching materials of biology laboratory management Saifuddin, Much Fuad; Puspitasari, Etika Dyah
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 6 No. 02 (2021): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v6i02.1958

The suitable teaching materials will make it easier for students to learn in the classroom. To present suitable teaching materials, a needs analysis is needed. This study aimed to determine the types and characteristics of teaching materials needed in learning biology laboratory management. The survey method was carried out to students who were randomly selected. The instrument is arranged in the form of a questionnaire by paying attention to each characteristic indicator of various teaching materials. The validity of the instrument used a combination of expert judgment. The questionnaire is distributed via google form to students who have taken laboratory management courses with a filling period of 2 weeks obtained 123 respondents. The data were analyzed descriptively of quantitatively and qualitatively. The results showed that the percentage of students who needed teaching materials for biology laboratory management lectures was 98%; the characteristics of teaching materials wanted by students included: containing learning objectives, using simple language, sequenced materials according to lecture sessions, containing summaries, and providing feedback. Teaching materials based on existing characteristics can be in the form of modules that are adapted to the Semester Learning Plan.

Effect of Fungicidal Methanol Extract of Kirinyuh Leaves (Eupatorium odoratum L.) and Noni Leaves (Morinda citrifolia L.) against MOLD Fusarium oxysporum on Tomatoes 'Ulya, Walidatul; Sofhiya, Evie Nur; Puspitasari, Etika Dyah
Biotik Vol 12 No 2 (2024): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v12i2.25335

Tomato plants are one of the leading horticultural commodities that are easily attacked by disease. Plant wilt disease is one of the diseases that often attacks tomato plants which is caused by Fusarium oxysporum. The use of chemical fungicides causes many losses, so there is a need for plant-based fungicides made from natural ingredients. Kirinyuh leaves and Noni leaves are natural ingredients that can be used as a vegetable fungicide to control fungi Fusarium oxysporum. The experiment was carried out alive and prepared using a Completely Randomized Design with 6 treatments and 6 replications. Data obtained from observations will be analyzed using the Duncan test at the 5% level. The concentrations used by each extract were 20%, 40%, 60%, and 80%, negative control (Aquadest), positive control (dithane M-45). The results showed that the Noni leaf extract fungicide with a concentration of 60% had the most effective effect in inhibiting the fungal growth zone Fusarium oxysporum on tomatoes.

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