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All Journal JURNAL FARMASIMED (JFM) Indonesia Natural Research Pharmaceutical Journal (INRPJ) Midang Indonesian Journal of Pharmaceutical Research
Djohansah, Violetta
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Religion Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Other

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Journal : JURNAL FARMASIMED (JFM)

 1 
Identification, Characterization and Antifungal Activity Test of Chemical Compounds from Methanol Extract of Macroalgae Padina australis Against Dandruff Fungus Malassezia globosa Djohansah, Violetta; Panjaitan, Riong Seulina; Zuraida Sagala
Jurnal FARMASIMED (JFM) Vol 8 No 1 (2025): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/3mat6358

Abstract

Padina australis is a species of brown macroalgae with potential as a natural source of bioactive compounds, including antifungal agents. This study aimed to identify the functional groups present in the methanolic extract of Padina australis using Fourier Transform Infrared (FTIR) spectroscopy and to evaluate its antifungal activity against Malassezia globosa through the disk diffusion method. FTIR analysis revealed the presence of hydroxyl (3433.41 cm⁻¹), aliphatic C–H (2916.47 cm⁻¹), carbonyl (1735.99 and 1643.41 cm⁻¹), amine (1573.97 and 1242.20 cm⁻¹), C–O, and carboxylate (COO⁻) groups, indicating key secondary metabolites such as flavonoids, phenols, esters, amines, and polysaccharides. Antifungal testing showed that the extract at a concentration of 100 ppm produced the largest inhibition zone (16.58 ± 4.71 mm), while the minimum inhibitory concentration (MIC) was determined at 12.5 ppm with an inhibition zone diameter ≥12 mm. These findings suggest that Padina australis holds promising potential as a natural source for developing antifungal therapies against Malassezia globosa.
Development and Method Validation of HPLC Method as an Key Method in the Analysis of Olopatadine HCl Handoyo, Theodorus Rexa; Djohansah, Violetta; Arbah, Windy Annisa
Jurnal FARMASIMED (JFM) Vol 8 No 1 (2025): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/68pp3f94

Abstract

Olopatadine hydrochloride (HCl) is a widely used second-generation antihistamine, and its accurate quantification is critical to ensure product quality and therapeutic efficacy. Reliable analytical methods are therefore essential for regulatory compliance and routine quality control. This study aimed to develop and validate a simple and robust high-performance liquid chromatography (HPLC) method for the determination of olopatadine HCl in pharmaceutical samples. The method was validated in accordance with USP <1225>, AOAC, and ICH Q2(R1) guidelines, with evaluation of key parameters including linearity, accuracy, precision, selectivity, sensitivity, and system suitability. The validation outcomes confirmed that the method provided strong linearity across the tested range, acceptable recovery values, excellent repeatability, and sufficient sensitivity for low-level detection. Overall, the developed HPLC method proved to be accurate, precise, selective, and reliable. Its simplicity and cost-effectiveness make it particularly suitable for routine quality control of olopatadine HCl in research and pharmaceutical industry laboratories.
Co-Authors Arbah, Windy Annisa Ardian, Kristian Derius Asriyanti, Laila Safa Derius Ardian, Kristian Fauziah Fauziah Kamaruddin, Muhammad Junaid Nabilah, Mei Syara Panjaitan, Riong seulina Parisa, Rika Rafael, Rafael Septiyani, Anisah Theodorus Rexa Handoyo Zuraida Sagala