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Yasinta Moi, Maria
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AKTIVITAS ANTIBAKTERI BUNGA GALA-GALA MERAH (SESBANIA GRANDIFLORA L.) TERHADAP PERTUMBUHAN BAKTERI STAPHYLOCOCCUS EPIDERMIDIS Yasinta Moi, Maria; Ulia Rita Sila, Vinsensia
Jurnal Biogenerasi Vol. 9 No. 2 (2024): Volume 9 Nomor 2 tahun 2024 periode (Juni 2024 - September 2024)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v9i2.4370

Abstract

Gala-gala is known to have many benefits as a medicinal plant that can cure some kinds of diseases. People in Timor Island, East Nusa Tenggara, use red gala-gala flowers as a source of vegetables and medicines to cure infectious diseases such as itching that causes abscesses. This study aims to find out the antibacterial activity of red gala-gala flower extract to inhibit the growth of Staphylococcus epidermidis bacteria. The method of antibacterial activity test used paper disk diffusion with concentrations of 20% and 40%. Antibacterial activities are shown by the presence of a halo zone around the agar surface that contains the test bacteria. The results were the average diameter of the barrier zone in 20% concentration at 9.11 mm, 40% at 10.76 mm, and the positive control at 30.46 mm. Also, there were no halo zones in the negative control. Based on the results, it can be concluded that red gala-gala flower extract possesses antibacterial activity in the sensitive category because it can inhibit the growth of bacteria within the diameter of the barrier zone produced by 10-15 mm. Thus, the red gala-gala flower can be used as one of the sources of natural bioactive anti-bacterial compounds to heal caused by microorganisms
AKTIVITAS ANTIBAKTERI BUNGA GALA-GALA MERAH (SESBANIA GRANDIFLORA L.) TERHADAP PERTUMBUHAN BAKTERI STAPHYLOCOCCUS EPIDERMIDIS Yasinta Moi, Maria; Ulia Rita Sila, Vinsensia
Jurnal Biogenerasi Vol. 9 No. 2 (2024): Volume 9 Nomor 2 tahun 2024 periode (Juni 2024 - September 2024)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v9i2.4370

Abstract

Gala-gala is known to have many benefits as a medicinal plant that can cure some kinds of diseases. People in Timor Island, East Nusa Tenggara, use red gala-gala flowers as a source of vegetables and medicines to cure infectious diseases such as itching that causes abscesses. This study aims to find out the antibacterial activity of red gala-gala flower extract to inhibit the growth of Staphylococcus epidermidis bacteria. The method of antibacterial activity test used paper disk diffusion with concentrations of 20% and 40%. Antibacterial activities are shown by the presence of a halo zone around the agar surface that contains the test bacteria. The results were the average diameter of the barrier zone in 20% concentration at 9.11 mm, 40% at 10.76 mm, and the positive control at 30.46 mm. Also, there were no halo zones in the negative control. Based on the results, it can be concluded that red gala-gala flower extract possesses antibacterial activity in the sensitive category because it can inhibit the growth of bacteria within the diameter of the barrier zone produced by 10-15 mm. Thus, the red gala-gala flower can be used as one of the sources of natural bioactive anti-bacterial compounds to heal caused by microorganisms
POTENSI TUMBUHAN BABOTEK (Cardiospernum halicacabum L.) SEBAGAI ANTIBAKTERI Yasinta Moi, Maria; Dorteia Da Cruz, Ijilda
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6651

Abstract

Babotek plant (Cardiospernum halicacabum L.) is one of the plants utilized by the ethnic community of Dualaus Village, Belu Regency, East Nusa Tenggara, as a traditional medicinal plant to treat diarrhea, flu, fever, and rheumatism. This research aims to test whether the Babotek plant has the ability to act as an antibacterial agent in inhibiting the growth of pathogenic bacteria. The antibacterial activity test method employed the disk diffusion method with plant extract concentrations of 10 ppm, 30 ppm, and 60 ppm, chloramphenicol antibiotic as a positive control, and Aquadest as a negative control. The extraction method used was a stepwise maceration method with ethyl acetate as the solvent. The research results obtained show that the ethyl acetate extract of the Babotek plant is capable of inhibiting the growth of the pathogenic bacteria Staphylococcus epidermidis, with an inhibition zone diameter of 8.2 mm at a concentration of 60 ppm; 7.8 mm at a concentration of 30 ppm; while no inhibition zone was formed at a concentration of 10 ppm. Antibacterial compounds produced by the Babotek plant in inhibiting the growth of test bacteria fall into the moderate category. This research is expected to contribute to efforts to improve public health quality and develop local community wisdom scientifically, as well as the sustainable use of plants.
ANALISIS KEBUTUHAN PENGEMBANGAN POSTER DIGITAL GLOSARIUM BAHASA LATIN MORFOLOGI TUMBUHAN SEBAGAI MEDIA PEMBELAJARAN BERBASIS CANVA Angel Masing, Feliksitas; Yasinta Moi, Maria; Felisima Tae, Lidwina
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4988

Abstract

This study aims to: 1) determine the process and obstacles faced by lecturers and students in learning plant morphology courses; 2) find out information on the use of learning media in plant morphology courses; 3) develop canva-based learning media in plant morphology courses. The subjects of this study were lecturers teaching plant morphology courses and semester III students of the Biology Education Study Program, Timor University. Data collection techniques were in the form of observation guidelines, interviews and documentation. The data analysis technique used was qualitative descriptive analysis. The results of the study showed that: 1) The process of learning plant morphology has been going well but there are still obstacles faced, namely difficulties in learning the Latin language of plant parts; 2) the learning media that have been used so far are print media, audio media, audio-visual media, but canva-based audio-visual media has never been used; 3) based on the results of the needs analysis, it is necessary to develop canva-based learning media in the form of a digital poster of a Latin glossary of plant morphology.
KARAKTERISASI DAN UJI KETAHANAN GARAM EMPEDU ISOLAT BAKTERI ASAM LAKTAT AIR RENDAMAN KACANG MERAH (Phaseolus Vulgaris) Ina Sintia Malo, Artin; Yasinta Moi, Maria
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8005

Abstract

Lactic Acid Bacteria (LAB) are a group of Gram-positive microorganisms that do not form spores and are non-motile. LAB includes microorganisms from a group of bacteria that play an important role in food fermentation by producing lactic acid that can inhibit the growth of pathogenic bacteria and food spoilage bacteria. The role of lactic acid bacteria as probiotics for human and animal health includes the digestive system, capable of acting as an antibiotic substitute, by maintaining microbial balance in the digestive system. Resistance to acid and bile salt is a prerequisite for a microorganism to be used as a probiotic because it indicates its ability to survive in the digestive tract. The objective of this research is to determine the characteristics of lactic acid bacteria isolates obtained from fermented red bean products and to know the resistance ability of lactic acid bacteria isolates to bile salt exposure. Isolation of Lactic Acid Bacteria was performed using the pour plate method on MRSA media. The bacteria were incubated at 37°C for 48 hours. Macroscopic observation was based on morphological characteristics consistent with lactic acid bacteria as commonly seen, which is determined by the color and shape of the colonies, while microscopic observation was focused on the shape and whether they are gram-positive or gram-negative. The results of the isolation from the red bean soaking water on MRSA media yielded one isolate that was successfully isolated from the red bean soaking water and was identified as lactic acid bacteria suspected to resemble the genus Streptococcus sp. The salt tolerance test of the lactic acid bacteria isolate KM2 from red bean soaking water showed an inability to survive in bile salt conditions. This indicates that the isolate does not have sufficient resilience to the bile-rich environment of the gastrointestinal tract.
Co-Authors Dorteia Da Cruz, Ijilda Feliksitas Angel Masing Felisima Tae, Lidwina Ina Sintia Malo, Artin Ulia Rita Sila, Vinsensia