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All Journal AL-HAYAT: Journal of Biology and Applied Biology Berkala Ilmiah Biologi
Rachmah, Annisa Nur
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Yeast and Wrapping Materials on The Quality of Tempeh Rachmah, Annisa Nur; Cempaka, Laras; Mukaromah, Arnia Sari
Berkala Ilmiah Biologi Vol 15 No 2 (2024)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v15i2.6341

Abstract

Abstract: Tempeh is one of the popular fermented soybeas products in Indonesia and in involves yeast in its manufacture. In addition to its delicious taste and relatively affordable price, tempeh has many nutritional values ​​including vitamin B6 (pyridoxine), vitamin K, protein and high essential amino acids. The good tempeh quality can be affected by materials preference and accuracy throughout making process. Besides soybeans, the tempeh quality is affected by type of yeast and packaging materials. Currently, tempeh making process commonly use instant yeast and plastic as wrapping material than either usar or other wrapping material in the market. The research objective was to elaborate the yeast types impact and wrapping materials on the quality and shelf life of soybean tempeh. The organoleptic assessment was carried out by the hedonic test analyzed by the Kruskal Wallis test and proceeded by the Mann Whitney test if there was a significant difference. Meanwhile, the observation of shelf life was carried out by means of a sensory test until the tempeh rotted. The results showed the type of yeast and packaging materials influencing on the quality of tempeh. Tempeh treatment P5 (using usar and banana leaves) had the highest score in color criteria (3.76±0.0737), smell (2.73±0.1447ceg), texture (2.93±0.1047ceg), taste (3.74±0.1407fj), shelf life (4 days) and faster mold growth than other treatment. According to the results, the panelists preferred tempeh fermented using usar and wrapped in banana leaves, because it was more natural and had the longest durability.
Authentication of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M Perry) Delima and Citra Cultivars by Morphological and Molecular Approach Mukaromah, Arnia Sari; Ulfah, Malia; Rachmah, Annisa Nur; Arfan, Muhammad Ramdhani; Kusumarini, Niken; Febriana, Asri
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.18713

Abstract

Wax apple Delima and Citra Cultivars are two superior non-climacteric tropical fruit commodities from Demak Regency which have similar morphological characters but have different fruit characteristics. Identification wax apple cultivars from Demak regency using DNA barcoding approach has not been researched yet.  The aims of this research are to analyze the morphological similarities of wax apple between Delima and Citra cultivars and to identify genetic variations of wax apple Delima and Citra using trnL-trnF intergenic spacer for molecularly authentication. The results shown that there were no differences in the environmental parameters of the wax apple two cultivated area in Demak Regency. According to the morphological approach, the Delima and Citra cultivars in Demak Regency were grouped separately into Delima and Citra clusters with a similarity index of 61.5% (Citra cultivars) and 60.5% (Delima cultivars) and separated based on the origin of their cultivation area. Genetic variations between wax apple Delima and Citra in Demak Regency consisted of deletions (9delA & 17delA) and conserved P6 loop in all compared cultivars. The possibility of heteroplasmy R (A,G) found at 73 nucleotide number in Delima Betokan cultivar and Citra cultivars (Betokan and Jungpasir). Meanwhile, Delima Jungpasir cultivar is K (G,T). There were P8 stem-loops with different lengths between Delima cultivars (Betokan and Jungpasir) and the same length in Citra cultivars (Betokan and Jungpasir). Therefore, the trnL-trnF intergenic spacer has not been thoroughly used in wax apple Delima and Citra authentication due to the presence of a secondary structure which causes the loss of the electropherogram signal so that the nucleotide sequence cannot be read.
Authentication of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M Perry) Delima and Citra Cultivars by Morphological and Molecular Approach Mukaromah, Arnia Sari; Ulfah, Malia; Rachmah, Annisa Nur; Arfan, Muhammad Ramdhani; Kusumarini, Niken; Febriana, Asri
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.18713

Abstract

Wax apple Delima and Citra Cultivars are two superior non-climacteric tropical fruit commodities from Demak Regency which have similar morphological characters but have different fruit characteristics. Identification wax apple cultivars from Demak regency using DNA barcoding approach has not been researched yet.  The aims of this research are to analyze the morphological similarities of wax apple between Delima and Citra cultivars and to identify genetic variations of wax apple Delima and Citra using trnL-trnF intergenic spacer for molecularly authentication. The results shown that there were no differences in the environmental parameters of the wax apple two cultivated area in Demak Regency. According to the morphological approach, the Delima and Citra cultivars in Demak Regency were grouped separately into Delima and Citra clusters with a similarity index of 61.5% (Citra cultivars) and 60.5% (Delima cultivars) and separated based on the origin of their cultivation area. Genetic variations between wax apple Delima and Citra in Demak Regency consisted of deletions (9delA & 17delA) and conserved P6 loop in all compared cultivars. The possibility of heteroplasmy R (A,G) found at 73 nucleotide number in Delima Betokan cultivar and Citra cultivars (Betokan and Jungpasir). Meanwhile, Delima Jungpasir cultivar is K (G,T). There were P8 stem-loops with different lengths between Delima cultivars (Betokan and Jungpasir) and the same length in Citra cultivars (Betokan and Jungpasir). Therefore, the trnL-trnF intergenic spacer has not been thoroughly used in wax apple Delima and Citra authentication due to the presence of a secondary structure which causes the loss of the electropherogram signal so that the nucleotide sequence cannot be read.
Co-Authors Arfan, Muhammad Ramdhani Arnia Sari Mukaromah Asri Febriana, Asri Laras Cempaka Niken Kusumarini, Niken Ulfah, Malia