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Kualitas semen segar sapi pejantan di Balai Besar Inseminasi Buatan Singosari Chika, Syifara; Febriana, Asri; Meilina, Tiara Dwi; Azzahro, Fathimah; Wulandari, Reza Ayuningtyas
Teknosains Vol 18 No 1 (2024): Januari-April
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v18i1.42626

Kebutuhan daging sapi di Indonesia meningkat setiap tahunnya, namun jumlah ketersediaan daging lebih rendah dibandingkan dengan jumlah permintaan konsumen. Salah satu upaya pemerintah dalam meningkatkan jumlah produktivitas dan populasi serta mutu genetik sapi yaitu menggunakan teknologi reproduksi melalui teknik inseminasi buatan. Penelitian ini bertujuan untuk mengetahui faktor-faktor yang memengaruhi kualitas semen segar sapi pejantan di BBIB Singosari. Metode penelitian ini bersifat kualitatif melalui wawancara kepada pengelola BBIB Singosari dan melalui kajian pustaka. Hasil penelitian diperoleh bahwa kualitas semen segar dipengaruhi oleh beberapa faktor, yaitu faktor bobot, musim, umur, kesehatan, suhu, dan pakan. Pada faktor bobot, berat badan sapi jantan berbanding lurus dengan ukuran testis dan peningkatan jumlah sperma. Pada faktor musim, nilai rerata konsentrasi spermatozoa pada musim kemarau lebih tinggi daripada musim penghujan. Faktor umur pada sapi pejantan dengan usia yang lebih matang akan menghasilkan jumlah semen yang lebih banyak dibandingkan dengan usia muda. Faktor kesehatan yaitu sapi pejantan harus bebas dari penyakit agar dapat menghasilkan semen segar yang berkualitas. Pada faktor suhu harus optimal agar menjaga libido sehingga kualitas semen bagus. Pada faktor pakan, nutrisi dalam pakan akan memengaruhi ukuran testis sapi pejantan.

psbA-trnH Intergenic Spacer profile of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M. Perry) Cultivars Meilina, Tiara Dwi; Chika, Syifara; Hariri, Muhammad Rifqi; Febriana, Asri; Mukaromah, Arnia Sari
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 4 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.4.548

The fruit known as wax apple, scientifically named Syzygium samarangense (Blume) Merr. & L.M. Perry, is a well-liked agricultural product originating from Demak Regency in the Central Java Province. When it is difficult to distinguish between different forms of an organism, using DNA barcoding to authenticate the identity of species based on their DNA is an important way. The psbA-trnH intergenic spacer is a commonly used molecular approach to analyse the genetic characteristics of plant species. This study investigated the psbA-trnH intergenic spacer profile of wax apple cultivars from Demak regency. The investigation revealed that the psbA-trnH intergenic spacer sequences of the S. samarangense cultivars, namely Madu Thailand and Madu Deli Hijau, had lengths of 535 and 492 base pairs, respectively. Both cultivars demonstrate a greater nucleotide composition of deoxyadenylic acid (A) and deoxythymidylic acid (T) in comparison to deoxycytidilic acid (C) and deoxyguanylic (G). The genetic distance between S. samarangense ‘Madu Thailand’ and ‘Madu Deli Hijau’ indicates a very tight relationship, with a value of 0.000. The psbA-trnH intergenic spacer proved to be insufficient in differentiating the S. samarangense cultivars from Demak regency, mostly because to its low capacity to discern between the wax apple cultivars. Keywords: Demak, psbA-trnH intergenic spacer, Syzygium samarangense, wax apple

NUTRITIONAL STATUS OF BAROMA RICE IN DIFFERENT COOKING METHODS Akmalia, Hafidha Asni; Febriana, Asri; Pranatami, Dwimei Ayudewandari; Adawiyah, Putri Rabiatul
Jurnal Gizi dan Pangan Soedirman Vol 8 No 2 (2024): Jurnal Gizi dan Pangan Soedirman
Publisher : Program Studi Ilmu Gizi, Jurusan Kesmas Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jgipas.2024.8.2.12808

Rice is one of the staple foods which is the main source nutrition such as carbohydrates, vitamins, minerals, protein, fat and fiber. The various rice cultivars that have been developed have different nutritional variations so that they provide choices according to the wishes of the community. This study aims to compare the profile of the compound content of Baroma rice in different cooking methods. Baroma rice was chosen in the study because the test for the content of compounds had never been carried out considering that the rice cultivar is a new type. The nutrients such as total protein, total fat, total sugar, starch, and calories were measured in the cooking method using stove and magic com with a completely randomized design and repeated 3 times. The results were analyzed using unpaired t-test analysis for the effect of different cooking methods. The result showed that magic com cooking increases fat and protein content while stove cooking elevates calories, starch, and total sugar.

Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.

Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.

Authentication of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M Perry) Delima and Citra Cultivars by Morphological and Molecular Approach Mukaromah, Arnia Sari; Ulfah, Malia; Rachmah, Annisa Nur; Arfan, Muhammad Ramdhani; Kusumarini, Niken; Febriana, Asri
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.18713

Wax apple Delima and Citra Cultivars are two superior non-climacteric tropical fruit commodities from Demak Regency which have similar morphological characters but have different fruit characteristics. Identification wax apple cultivars from Demak regency using DNA barcoding approach has not been researched yet. The aims of this research are to analyze the morphological similarities of wax apple between Delima and Citra cultivars and to identify genetic variations of wax apple Delima and Citra using trnL-trnF intergenic spacer for molecularly authentication. The results shown that there were no differences in the environmental parameters of the wax apple two cultivated area in Demak Regency. According to the morphological approach, the Delima and Citra cultivars in Demak Regency were grouped separately into Delima and Citra clusters with a similarity index of 61.5% (Citra cultivars) and 60.5% (Delima cultivars) and separated based on the origin of their cultivation area. Genetic variations between wax apple Delima and Citra in Demak Regency consisted of deletions (9delA & 17delA) and conserved P6 loop in all compared cultivars. The possibility of heteroplasmy R (A,G) found at 73 nucleotide number in Delima Betokan cultivar and Citra cultivars (Betokan and Jungpasir). Meanwhile, Delima Jungpasir cultivar is K (G,T). There were P8 stem-loops with different lengths between Delima cultivars (Betokan and Jungpasir) and the same length in Citra cultivars (Betokan and Jungpasir). Therefore, the trnL-trnF intergenic spacer has not been thoroughly used in wax apple Delima and Citra authentication due to the presence of a secondary structure which causes the loss of the electropherogram signal so that the nucleotide sequence cannot be read.

Authentication of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M Perry) Delima and Citra Cultivars by Morphological and Molecular Approach Mukaromah, Arnia Sari; Ulfah, Malia; Rachmah, Annisa Nur; Arfan, Muhammad Ramdhani; Kusumarini, Niken; Febriana, Asri
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.18713

Wax apple Delima and Citra Cultivars are two superior non-climacteric tropical fruit commodities from Demak Regency which have similar morphological characters but have different fruit characteristics. Identification wax apple cultivars from Demak regency using DNA barcoding approach has not been researched yet. The aims of this research are to analyze the morphological similarities of wax apple between Delima and Citra cultivars and to identify genetic variations of wax apple Delima and Citra using trnL-trnF intergenic spacer for molecularly authentication. The results shown that there were no differences in the environmental parameters of the wax apple two cultivated area in Demak Regency. According to the morphological approach, the Delima and Citra cultivars in Demak Regency were grouped separately into Delima and Citra clusters with a similarity index of 61.5% (Citra cultivars) and 60.5% (Delima cultivars) and separated based on the origin of their cultivation area. Genetic variations between wax apple Delima and Citra in Demak Regency consisted of deletions (9delA & 17delA) and conserved P6 loop in all compared cultivars. The possibility of heteroplasmy R (A,G) found at 73 nucleotide number in Delima Betokan cultivar and Citra cultivars (Betokan and Jungpasir). Meanwhile, Delima Jungpasir cultivar is K (G,T). There were P8 stem-loops with different lengths between Delima cultivars (Betokan and Jungpasir) and the same length in Citra cultivars (Betokan and Jungpasir). Therefore, the trnL-trnF intergenic spacer has not been thoroughly used in wax apple Delima and Citra authentication due to the presence of a secondary structure which causes the loss of the electropherogram signal so that the nucleotide sequence cannot be read.

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