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All Journal IMJ (Indonesian Midwifery Journal) Pelita Eksakta Promotor: Jurnal Mahasiswa Kesehatan Masyarakat
Sefina, Nadia
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Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Mathematics Medicine & Pharmacology Nursing Physics Public Health Other

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Pengaruh Kandungan Ekstrak Gel Lidah buaya (Aloe vera L.) terhadap Kualitas Spermatozoa Mencit Jantan (Mus musculus L.) dalam Penerapan Etnosains Sefina, Nadia
IMJ (Indonesian Midwifery Journal) Vol 7, No 2 (2024): IMJ (Indonesia Midwifery Journal) Februari - Agustus 2024
Publisher : Universitas Muhammadiyah Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31000/imj.v7i2.8817

Abstract

Salah satu tumbuhan yang berpotensi sebagai obat yaitu lidah buaya (Aloe vera L.). Kandungan senyawa aktif pada daun lidah buaya diduga berpengaruh terhadap proses spermatogenesis dan pematangan sperma. Senyawa aktif ini termasuk tanin, saponin, dan flavonoid. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak gel lidah buaya (Aloe vera L.) terhadap kualitas spermatozoa mencit jantan (Mus musculus L.) dalam penerapan etnosains. Penelitian dilakukan di Laboratorium Biologi,  Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang dimulai pada tanggal 2 Maret 2023 sampai dengan 6 April 2023. Metode penelitian yang digunakan adalah eksperimental dengan 3 kelompok perlakuan dan 5 ulangan, P0 sebagai kontrol sehingga tidak dilakukan pemberian ekstrak, P1 diberikan ekstrak gel lidah buaya dosis 0,5 ml dengan konsentrasi gel 100 % dan P2 diberikan ekstrak gel lidah buaya dosis 0,5 ml dengan campuran konsentrasi gel lidah buaya 50 % dan aquades 50 %. Penelitian ini dianalisis menggunakan analisis deskriptif dalam bentuk tabel yang memberikan gambaran secara umum mengenai kualitas spermatozoa yang diberikan ekstrak gel lidah buaya (Aloe vera L.). Pemberian ekstrak gel lidah buaya (Aloe vera L.) memberikan hasil penurunan kualitas spermatozoa dilihat dari morfologi yang abnormal dan motilitas spermatozoa yang lambat. Kata kunci Aloe vera, Mus musculus, Spermatozoa, Morfologi, Mortilitas
Assistance and Capacity Building for Entrepreneurship through Optimization of Field and Sea Products Based on Community Circular Economy Ahda, Yuni; Syahrastani, Syahrastani; Faridah, Anni; Razak, Abdul; Pratama, Sandi Fransisco; Farma, Siska Alicia; Sagurung, Mariance Dartiani; Sefina, Nadia; Valofi, Nagra Aulia
Pelita Eksakta Vol 8 No 01 (2025): Pelita Eksakta, Vol. 8, No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss01/266

Abstract

The village of Muara Sikabaluan in the Mentawai Islands faces various challenges in education, economy, and the environment. A community empowerment program based on the circular economy has been implemented to enhance the capacity for processing agricultural and marine products, such as bananas, taro, cassava, and fish. Training in product processing and online and offline marketing was provided to improve the knowledge and skills of residents, especially housewives. The program also promotes the establishment of a village business and economic center as a hub for business development and community welfare improvement. Pretest and posttest results showed significant increases in residents' knowledge and skills related to product processing and the application of circular economy principles. With a participatory and sustainable approach, Muara Sikabaluan Village is expected to become a model for sustainable village development.
Optimisation of 25-OH D Elisa Kit Usage Using EDTA, Heparin, and Gel Blood Tubes Sefina, Nadia; Achyar, Afifatul; Wahyuni, Ira
PROMOTOR Vol. 8 No. 5 (2025): OKTOBER
Publisher : Universitas Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/pro.v8i5.1388

Abstract

The use of anticoagulants in blood collection tubes is also an important factor in the measurement of vitamin D and its metabolites. Vitamin D levels in circulating blood can be measured by measuring 25-hydroxyvitamin D levels in blood. This study aims to compare the effectiveness of using EDTA, heparin, and gel blood tubes in maintaining the stability of 25-OH D in blood samples. The type of research used is Analytical Descriptive research. The samples used in this study used venous blood which was determined using the Simple Random Sampling technique. Samples were taken from 5 semester S1 internship students totalling three people to take blood. Where the blood was taken as much as 3 cc and divided into several purple, green and yellow vacuntainer tubes. From the data obtained, it shows that the use of vacutainer tubes in blood storage to obtain serum for the most optimum vitamin D level test is in a tube with a green lid containing lithium heparin anticoagulant. Where the results obtained on lithium heparin anticoagulant are in the range of >60 ng/mL. As strong evidence of no potential adverse effects at high (optimal) levels. Tubes with purple or lavender coloured covers containing EDTA had results <20 ng/mL indicating that vitamin D deficiency may occur due to lack of vitamin D nutrition. On the other hand, the tube with yellow coloured lid containing anticoagulant gel showed >100 ng/mL indicating vitamin D excess.
Co-Authors Abdul Razak Achyar, Afifatul Anni Faridah Pratama, Sandi Fransisco Sagurung, Mariance Dartiani Syahrastani, Syahrastani Valofi, Nagra Aulia Yuni Ahda