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In Vitro Anthelmintic Evaluation of Acanthophora spicifera Macroalgal Extract Against Haemonchus contortus in Sheep Sakti, A. A.; Kustantinah; Suwignyo, B.; Sofyan, A.; Panjono; Nurcahyo, R. W.; Baihaqi, Z. A.; Harahap, M. A.; Wulandari; Prasetyo
Tropical Animal Science Journal Vol. 48 No. 6 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.6.508

Abstract

This study investigated the chemical composition and anthelmintic activity of Acanthophora spicifera against Haemonchus contortus from sheep in vitro. Fresh macroalgae were collected from Sepanjang Beach, Gunungkidul, Indonesia. Proximate analysis, fiber fractions, mineral composition, and color profile analysis were conducted to determine chemical profile of the macroalga. It was extracted by maceration with 96% ethanol (1:5) and ultrasonication. Secondary metabolites analyzed from the extract included total flavonoids, phenols, tannins, and saponins. The anthelmintic activity was tested in vitro through H. contortus adult worm motility and egg hatch inhibition tests. Extract treatments included P0: 0.9% physiological NaCl (negative control); P1: 0.5 mg/mL macroalgal extract; P2: 1 mg/mL macroalgal extract; P3: 1.5 mg/mL macroalgal extract; and Palb: 0.5 mg/mL albendazole (positive control). Motility test results showed that the use of macroalgae at levels of 0.5, 1, and 1.5 mg/mL significantly reduced H. contortus motility (p<0.05). However, effective LD₅₀ was reached in 1 and 1.5 mg/mL within the first 10 hours, and LD₁₀₀ after 21 hours (p<0.05). None of the extract levels matched the efficacy of albendazole (p<0.05). The egg hatch inhibition test revealed that the use of macroalgae at levels of 0.5, 1, and 1.5 mg/mL significantly inhibited H. contortus egg hatching by more than 85.73% after 24 hours (p<0.01), demonstrating comparable efficacy to albendazole. Based on these findings, A. spicifera extract contains various secondary metabolites with anthelmintic activity, inhibiting both H. contortus adult worm motility and egg hatching. This suggests that A. spicifera has potential for further development as a bioanthelmintic for ruminant parasite control, although further in vivo studies are needed.
Effect of Shearing on Thermo-Physiological, Behavior, and Productivity Traits of Two Indonesian Local Sheep Breeds Panjono; Ibrahim, A.; Ngadiyono, N.; Maulana, H.; Atmoko, B. A.
Tropical Animal Science Journal Vol. 47 No. 1 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.1.42

Abstract

Thin-tailed sheep (TTS) and Fat-tailed sheep (FTS) are local Indonesian sheep breeds characterized by coarse wool. This study aimed to investigate the effects of wool shearing on the thermo-physiological, behavior, and productivity traits of these sheep. Sixteen selected rams were utilized in this study. Animals were assigned to a factorial completely randomized design and divided into two groups (TTS and FTS) and two treatments (sheared and unsheared). The study spanned three months under controlled conditions. Variables observed included environmental conditions, thermo-physiological parameters (respiratory rate/RR, pulse rate/PR, rectal temperature/RT, and heat stress index/HSI), sheep behavior (feeding duration, drinking frequency, rumination duration, urination frequency, defecation frequency, standing duration, and lying duration), and sheep productivity (feed intake, average daily gain/ADG, and feed conversion ratio/FCR). Data were analyzed using two-way ANOVA. Throughout the study, average temperature and humidity ranged from 25.13-30.48 oC and 64.50%-91.67%, respectively. Wool shearing significantly influenced (p<0.05) sheep’s thermo-physiological, behavior, and productivity traits. These effects were consistent across sheep breeds, with no significant differences noted. Wool shearing significantly reduced (p<0.05) RR, PR, and RT, while the impact on average HSI was not significant. Additionally, sheared sheep exhibited increased (p<0.05) feeding, rumination, standing duration, and higher defecation frequency. Conversely, drinking frequency, urination frequency, and lying duration decreased in the sheared sheep group. Moreover, the sheared sheep demonstrated higher (p<0.05) feed intake and ADG, leading to a reduced (p<0.05) FCR compared to the unsheared group. In conclusion, shearing is a recommended practice for coarse wool-type sheep in tropical environments. This technique does not induce stress and enhances their thermo-physiological, behavior, and productivity traits.
Perceptions and Economic Losses of Foot and Mouth Disease to Beef Cattle Farmers in Bantul Regency, Yogyakarta Putra, D. W.; Kusumastuti, T. A.; Andarwati, S.; Panjono; Guntoro, B.
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.529

Abstract

The objectives of this study were to measure the level of farmers’ perceptions on psychological, socio-cultural and economic aspects, and to analyze the economic losses during foot and mouth disease (FMD) outbreaks. This study was conducted in Bantul District, Yogyakarta, during the period of July-September 2023. A total of 148 farmers affected by FMD were surveyed by a purposive sampling method using a questionnaire and the data were analyzed using the ordinal logistic regression method. The result showed that farmers’ perceptions of FMD outbreaks of psychological, socio-cultural and economic attributes were in the high category. The majority of farmers experienced low economic losses (47.30%). Age and livestock ownership (p<0.01), formal education, and informal education (p<0.1) had a positive effect on the level of farmers’ economic losses, while only farming experience (p<0.01) had a negative effect. Pseudo R-Square value of 33.9% or the level of economic losses, is influenced by farmers’ age, farming experience, livestock ownership, as well as formal and informal education. Reaction in the field of opportunity: the highest level of economic losses in the low category is 0.997%. This study shows that FMD handling carried out by farmers is good enough to prevent the impact of large economic losses on cattle farmers in Bantul Regency.