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All Journal Jurnal Sains dan Teknologi Biosaintifika: Journal of Biology & Biology Education
Putu Sathiya Adi Janendra
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Agriculture, Biological Sciences & Forestry Computer Science & IT Education

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Optimizing Canang Flower Waste Extract for Staining Fasciolopsis buski Eggs: An Enhancement of the Kato-Katz Method I Komang Tri Yasa Widnyana; Kadek Indira Maheswari; Putu Sathiya Adi Janendra; Dewa Gede Putra Mahayana; Indra Dwisaputra; Made Bayu Permasutha; Irma Rahmayani; Pasala, Metamalik
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22829

Abstract

Fasciolopsis buski is a parasitic helminth that can infect humans. Diagnosing helminthiasis can be confirmed through fecal testing utilizing the Kato-Katz method.  The Kato-Katz method employs methylene blue staining, which poses environmental hazards due to its carcinogenic characteristics. An alternative to staining is the use of a canang, a common Balinese item used for prayers. The utilized waste canang flowers were Tagetes erecta, Impatiens balsamina (red), and Impatiens balsamina (purple). The research began with an extraction procedure that involved cutting the flowers into small pieces, resulting in approximately 500 grams of fragments. The flower components were macerated in two liters of 96% ethanol for five days. Additionally, cellophane immersion of the extracted findings was performed. The findings indicated that each sample from the three treatment groups (T1, 1%; T2, 2%; and T3, 3%) and the two control groups.  Sub-analysis testing evaluated the quantity of helminth eggs, quantified as eggs per gram of feces via field-of-view observation. The T3 (3%) exhibited results that were not statistically significantly different (P>0.05) from the positive control group. The T3 (3%) test provides the most favorable and optimal results as a substitute for methylene blue in microscopic staining evaluations.
POTENSI FETAL BOVINE SERUM PADA MODIFIKASI MEDIUM KULTUR JONES DALAM DIAGNOSIS Blastocystis hominis: STUDI MENGENAI GAMBARAN MORFOLOGI DAN UJI PH Kadek Intan Arta Sarita; Putu Sathiya Adi Janendra; Kadek Indira Maheswari; Kadek Edy Sukarma; Made Bayu Permasutha; Made Kurnia Widiastuti Giri
JST (Jurnal Sains dan Teknologi) Vol. 13 No. 1 (2024): April
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v13i1.78206

Abstract

Diarrhea is the third leading cause of death among children under the age of five worldwide. Diarrhea can result from bacterial infections, viral infections, or parasitic infestations that disrupt the functioning of the digestive system. Therefore, research on the morphology of Blastocystis hominis is necessary to aid in the diagnosis of diarrhea. This study is an experimental investigation conducted on Jones culture medium that has been altered with fetal bovine serum. The study consists of five trial groups with different volume: P1 (0.1 mL), P2 (0.5 mL), P3 (1.0 mL), P4 (1.5 mL), and P5 (2.0 mL). The morphological findings of Blastocystis hominis in each trial group, with pH averages P1 (7.29), P2 (7.27), P3 (7.30), dan P4 (7.31), were examined microscopically. The results were found to be optimal, with a statistical value of P>0.05 in the independent t-test. The P5 test group (6.15) differed significantly from the gold standard at P<0.001. The fetal bovine serum can be utilized for clinical reasons at a volume of 0.1 mL and a pH of 7.29, as it is already capable of displaying morphological results.
Co-Authors Dewa Gede Putra Mahayana I Komang Tri Yasa Widnyana Indra Dwisaputra Irma Rahmayani Kadek Edy Sukarma Kadek Indira Maheswari Kadek Intan Arta Sarita Made Bayu Permasutha Made Kurnia Widiastuti Giri Pasala, Metamalik