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Screening of Extract Secondary Metabolites of Bacteria Which Have Symbiosis with Sponges from Central Tapanuli Bakar Island as an Antibacteria mirnawati, mirnawati; Rasyidah; Mayasari , Ulfayani; Gultom, Endang Sulistyarini
International Journal of Ecophysiology Vol. 6 No. 2 (2024): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v6i1.17821

This study aims to determine the presence of antibacterial-producing bacteria from sponges from Bakar Island, Central Tapanuli Regency and to determine the antibacterial activity of methanol extracts from potential bacterial isolates against Staphylococcus aureus and Escherichiacoli. 21 pure isolates were found and it was known that 9 isolates produced antibacterial against S. aureus and 4 isolates against E. coli. Bacterial isolates with the highest inhibition zone against bothS.aureus and E. coli were found in isolates with code SP20 with each large inhibition zone are 14.5 mm and 16.04 mm. Then microscopic observations were carried out, namely gram staining and were obtained 8 isolates were gram positive, and 1 gram was negative. The biochemical test showed that SP20 showed positive results for the Motility, TSIA and Catalase Test but negative for Citrate Test. Bacterial isolates withthe code SP20 had the largest inhibition zone so that these isolates were to be extracted.The resultsof the screening test showed that the secondary metabolitesof the sponge symbiont bacterial extract from Bakar Island were positive for flavonoids and saponins. And then test the activity of secondary metabolites with 3 concentrations, 10%, 20%, and 30%.The results showed that the best concentration of the test bacteria was S. aureus at a concentration of 30% with an inhibition zone of 3.08 mm and E. coli at 5.04 mm.

FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter

UJI AKTIVITAS ANTI BAKTERI MADU TRIGONA TERHADAP BAKTERI Escherichia coli dan Klebsiella pneumoniae Gultom, Endang Sulistyarini; Pohan, Selvia Dewi; Situmorang, Nurbaity; Hafzari, Rini
Eksakta : Jurnal Penelitian dan Pembelajaran MIPA Vol 10, No 1 (2025): Eksakta : Jurnal Penelitian dan Pembelajaran MIPA
Publisher : Fakultas Keguruan Dan Ilmu Pendidikan, UM-Tapsel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/eksakta.v10i1.299-304

Antibiotic resistance has the greatest impact on human health, at least 2 million people are infected by antibiotic-resistant bacteria. The use of natural medicines is increasingly in demand by the public, one of which is often used to treat diseases is trigona honey. This study aims to determine the antibacterial activity of Trigona honey against Escherichia coli and Klebsiella pneumoniae bacteria. The test bacteria used were Multidrug Resistant Escherichia coli and Klebsiella pneumoniae bacteria obtained from the collection of the Microbiology Laboratory of the University of North Sumatera Hospital. Antimicrobial activity testing was carried out using the hole / well diffusion method on Mueller Hinton Agar (MHA) media with chloramphenicol as a positive control, acetone as a negative control and trigona honey with a concentration of 500 ppm. Antibacterial activity was seen by measuring the inhibition zone formed. The results showed that Trigona honey was able to inhibit the growth of Escherichia coli and Klebsiella pneumoniae bacteria with inhibition zone activity included in the weak category, while the positive control showed antibacterial activity in the strong category.

Profil Pertumbuhan dan Produksi Mutan Bawang Putih (Allium sativum L.) Kultivar Doulu Generasi MV5 Marbun, Jumita Sari; Gultom, Endang Sulistyarini
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 1 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i1.2291

Penelitian ini bertujuan untuk mengetahui pengaruh radiasi sinar gamma terhadap pertumbuhan dan produksi dari tanaman bawang putih (Allium sativum L.) kultivar Doulu pada generasi Mutasi Vegetatif ke 5. Penelitian ini dilakukan selama 4 bulan dari tanggal 3 September 2022 - 2 Januari 2023. Penelitian ini dilaksanakan di Desa Sionggang Utara, Kabupaten Toba Samosir, Sumatera Utara menggunakan 240 sampel siung bawang putih (Allium sativum L.) kultivar Doulu generasi MV4. Rancangan yang digunakan dalam penelitian ini yaitu Rancangan Acak Kelompok (RAK), menggunakan uji AVONA dan memakai uji lanjut BNJ 5%. Dosis perlakuan dari sinar gamma ini adalah G0 (kontrol), G1 (2 Gy), G2 (4 Gy), G3 (6 Gy), G4 (8 Gy), dan G5 (10 Gy). Parameter yang diamati yaitu umur tumbuh, jumlah daun, diameter umbi, berat umbi, jumlah siung, berat siung, dan diameter siung. Susunan daun pada tanaman bawang putih sama yaitu rendah atau low. Struktur umbi bawang putih nya regular two-fan groups, regular multi-cloved radial, regular multi-fan groups, dan irregular. Pertumbuhan bawang putih pada dosis 4 Gy yang lebih baik untuk digunakan. Sedangkan untuk umur tumbuh (4 Gy), jumlah daun (10 Gy), diameter umbi (4 Gy), berat umbi (4 Gy), jumlah siung (8 Gy), berat siung (4 Gy), dan diameter siung (4 Gy).

IMPLEMENTATION OF LEARNING BASED ON E-WORKSHEETS WITH A SCIENTIFIC APPROACH ON THE MATERIAL OF THE DIGESTIVE SYSTEM IN CLASS XI SMA UNGGULAN AL-MANAR Gultom, Endang Sulistyarini; Yenda, Azura Nur
Jurnal Pelita Pendidikan Vol. 13 No. 1 (2025): JURNAL PELITA PENDIDIKAN
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar

This study aims to determine the application of e-worksheet with a scientific approach in class XI IPA SMA Unggulan Al-Manar in the learning process as measured by students' critical thinking skills. The instrument for measuring the question is administered both before and after the implementation of the learning. The data analysis technique employs quantitative analysis, specifically the calculation of the average value, to assess the students' critical thinking skills. The results of the data analysis indicate that the implementation of learning using e-worksheet with a scientific approach can enhance the critical thinking skills of students in class XI IPA at SMA Unggulan Al-Manar. This is evidenced by the change in the cognitive value of students during the pretest and posttest, which fell within the very good category. The average value of students during the pretest was 54.13, while the average value increased to 86.75 during the posttest, indicating an improvement in critical thinking skills.

Identification of Bacterial Symbiont Sponge with Antibacterial Activity against Multi Drug Resistant Organism (MDRO) Bacteria from Sea Waters in Sibolga, North Sumatra Indonesia Gultom, Endang Sulistyarini; Hasruddin, Hasruddin; Sitompul, Aida Fitriani; Situmorang, Albert D; Prasetya, Eko
Biosfer: Jurnal Tadris Biologi Vol 12 No 2 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i2.10138

This research employed the experimental method with descriptive data analysis. It aimed to isolate and identify sponge symbiont bacteria with antibacterial potential against Multi-Drug Resistance Organisms (MDRO) with the 16S rRNA gene marker. Based on the study results, two sponges were obtained, namely Clathrina sp and Agelas sp, each having 11 and 7 isolates of symbiont bacteria, respectively. Based on the antibacterial activity test results, out of 18 isolates of symbiont bacteria, only 4 had antibacterial potential against MDRO (Klebsiella pneumoniae ESBL, Pseudomonas aeruginosa ESBL, or Staphylococcus lugdunensis MRSA) with inhibition zones of 9.3 mm, 8.3 mm, and 8.1 mm, respectively. The results of bacterial identification using the 16S rRNA gene sequence isolate S1I3, S1I9, and A1I3 belong to the Bacillus cereus and Bacillus paramycoides types. This research is expected to provide important information about sponge symbiont bacteria with antibacterial potential against MDRO.

PEMBERDAYAAN MASYARAKAT DESA PARBABA DOLOK, KABUPATEN SAMOSIR, SUMATERA UTARA MELALUI INOVASI PENGOLAHAN LIMBAH KULIT KOPI (CASCARA) MENJADI TEH HERBAL Hafzari, Rini; Gultom, Endang Sulistyarini
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol. 31 No. 4 (2025): Oktober-Desember
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkm.v31i4.69709

Program pengabdian kepada masyarakat ini dilaksanakan di Desa Parbaba Dolok, Kabupaten Samosir, Sumatera Utara yang dikenal sebagai sentra produksi kopi. Selama ini, pemanfaatan hasil kopi masih terbatas pada biji, sementara limbah kulit kopi (cascara) belum dimanfaatkan optimal dan seringkali menimbulkan pencemaran lingkungan. Padahal cascara mengandung serat, protein, dan senyawa bioaktif yang berpotensi diolah menjadi produk minuman fungsional. Kegiatan ini bertujuan untuk memberdayakan kelompok tani kopi melalui penerapan teknologi tepat guna dalam mengolah cascara menjadi teh herbal bernilai ekonomi serta mendampingi masyarakat dalam aspek produksi, pengemasan, dan pemasaran digital. Metode yang dilakukan meliputi sosialisasi, pelatihan teknis pengolahan cascara (pencucian, pengeringan dengan food dehydrator, pencacahan, dan pengemasan), demonstrasi penggunaan alat sederhana, workshop desain kemasan dan branding, serta pelatihan pemasaran berbasis media sosial dan marketplace. Evaluasi program dilaksanakan melalui observasi langsung, dokumentasi kegiatan, uji organoleptik sederhana, dan analisis peningkatan kapasitas mitra. Hasil program menunjukkan peningkatan keterampilan masyarakat dalam mengolah cascara menjadi teh herbal siap konsumsi, meningkatnya pemahaman mengenai standar mutu dan kebersihan, serta adanya kemampuan baru dalam pemasaran digital. Luaran yang dicapai berupa produk teh herbal cascara dalam kemasan, publikasi di media massa, video dokumentasi, draft artikel, dan pendaftaran paten sederhana/HKI. Program ini membuktikan bahwa pemanfaatan limbah kopi dengan teknologi tepat guna dapat mengurangi pencemaran, meningkatkan pendapatan masyarakat, serta memperkuat identitas produk lokal.

FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter

Potential of Fig Fruit Ethanol Extract (Ficus carica L.) Against Staphylococcus aureus MRSA and Klebsiella pneumoniae ESBL Bacteria Padang, Enmia Septia; Hafzari, Rini; Gultom, Endang Sulistyarini
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1033-1038

Ficus carica L. commonly known as figs originate from West Asia and have bioactive components with antibacterial properties. This study aimed to determine the antibacterial activity of fig ethanol extract in inhibiting the growth of MDR Staphylococcus aureus MRSA and Klebsiella pneumoniae ESBL bacteria, to determine the MIC and MBC values, and the types of compounds contained in fig ethanol extract. The method began with fig extraction using concentrations of 20%, 40%, 60%, 80%, and 100% with 4 repetitions, testing MIC and MBC using the liquid dilution method, TLC-Bioautography test with ethyl acetate: methanol: aquades (7: 2: 1) eluent. The results showed that fig fruit extract had an inhibition zone with an average value of 3.30 mm at a concentration of 100% which was still in the weak category, the MIC value of Staphylococcus aureus MRSA bacteria was a concentration of 100% and Klebsiella pneumoniae ESBL bacteria was a concentration of 80%. The MBC value of MDR bacteria was not obtained because there was still bacterial growth. The results of the TLC-bioautography test of ethanol extract of fig fruit (Ficus carica L.) can inhibit bacterial growth indicated by the presence of a clear zone with an Rf value of 0.5 cm (unidentified), 0.31 cm (Flavonoid), 0.2 cm (Phenol). The compounds that act as antibacterials against Staphylococcus aureus MRSA and Klebsiella pneumoniae ESBL bacteria are the flavonoid and phenol groups.

Uji Aktivitas Antibakteri Ekstrak Etanol Daun Senduduk (Melastoma Malabathricum Lour.) dalam Menghambat Pertumbuhan Bakteri pada Saluran Hidung: Penelitian Wulandari, Atika; Manurung, Dhea Amelia; Baringbing, Siska Juliana; Zain, Ulfi Khairani; Gultom, Wirda Resinta; Gultom, Endang Sulistyarini; Situmorang, Nurbaity
Jurnal Pengabdian Masyarakat dan Riset Pendidikan Vol. 4 No. 2 (2025): Jurnal Pengabdian Masyarakat dan Riset Pendidikan Volume 4 Nomor 2 (October 202
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jerkin.v4i2.3524

This study aims to determine the antibacterial activity of ethanol extract of senduduk leaves (Melastoma malabathricum Lour.) in inhibiting bacterial growth in the nasal passages. Senduduk plants contain bioactive compounds such as flavonoids, tannins, and saponins that have the potential as natural antibacterials. The study was conducted experimentally in vitro at the Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, State University of Medan in September 2025 using the disc diffusion method. Senduduk leaf extraction was carried out by maceration using 96% ethanol. The positive control used was the antibiotic ciprofloxacin HCl, while the negative control used DMSO (Dimethyl Sulfoxide). The growth medium used was Mueller Hinton Agar (MHA), and antibacterial activity was measured based on the diameter of the inhibition zone around the test disc. The results showed that senduduk leaf extract produced an average inhibition zone of 9.61 mm, while ciprofloxacin HCl produced an inhibition zone of around 28.45 mm, and DMSO showed no inhibition zone. This study is the basis for the development of senduduk leaves as a potential natural antibacterial ingredient in the pharmaceutical and public health fields.

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