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All Journal JBIO: jurnal biosains (the journal of biosciences) Jurnal Pelita Pendidikan Biosfer: Jurnal Tadris Biologi EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA IJOEP : International Journal of Ecophysiology BIO-CONS: Jurnal Biologi dan Konservasi
Gultom, Endang Sulistyarini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Energy Immunology & microbiology Medicine & Pharmacology Social Sciences Other

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Screening of Extract Secondary Metabolites of Bacteria Which Have Symbiosis with Sponges from Central Tapanuli Bakar Island as an Antibacteria mirnawati, mirnawati; Rasyidah; Mayasari , Ulfayani; Gultom, Endang Sulistyarini
International Journal of Ecophysiology Vol. 6 No. 2 (2024): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v6i1.17821

Abstract

This study aims to determine the presence of antibacterial-producing bacteria from sponges from Bakar Island, Central Tapanuli Regency and to determine the antibacterial activity of methanol extracts from potential bacterial isolates against Staphylococcus aureus and Escherichiacoli. 21 pure isolates were found and it was known that 9 isolates produced antibacterial against S. aureus and 4 isolates against E. coli. Bacterial isolates with the highest inhibition zone against bothS.aureus and E. coli were found in isolates with code SP20 with each large inhibition zone are 14.5 mm and 16.04 mm. Then microscopic observations were carried out, namely gram staining and were obtained 8 isolates were gram positive, and 1 gram was negative. The biochemical test showed that SP20 showed positive results for the Motility, TSIA and Catalase Test but negative for Citrate Test. Bacterial isolates withthe code SP20 had the largest inhibition zone so that these isolates were to be extracted.The resultsof the screening test showed that the secondary metabolitesof the sponge symbiont bacterial extract from Bakar Island were positive for flavonoids and saponins. And then test the activity of secondary metabolites with 3 concentrations, 10%, 20%, and 30%.The results showed that the best concentration of the test bacteria was S. aureus at a concentration of 30% with an inhibition zone of 3.08 mm and E. coli at 5.04 mm.
FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

Abstract

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter
UJI AKTIVITAS ANTI BAKTERI MADU TRIGONA TERHADAP BAKTERI Escherichia coli dan Klebsiella pneumoniae Gultom, Endang Sulistyarini; Pohan, Selvia Dewi; Situmorang, Nurbaity; Hafzari, Rini
Eksakta : Jurnal Penelitian dan Pembelajaran MIPA Vol 10, No 1 (2025): Eksakta : Jurnal Penelitian dan Pembelajaran MIPA
Publisher : Fakultas Keguruan Dan Ilmu Pendidikan, UM-Tapsel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/eksakta.v10i1.299-304

Abstract

Antibiotic resistance has the greatest impact on human health, at least 2 million people are infected by antibiotic-resistant bacteria. The use of natural medicines is increasingly in demand by the public, one of which is often used to treat diseases is trigona honey. This study aims to determine the antibacterial activity of Trigona honey against Escherichia coli and Klebsiella pneumoniae bacteria. The test bacteria used were Multidrug Resistant Escherichia coli and Klebsiella pneumoniae bacteria obtained from the collection of the Microbiology Laboratory of the University of North Sumatera Hospital. Antimicrobial activity testing was carried out using the hole / well diffusion method on Mueller Hinton Agar (MHA) media with chloramphenicol as a positive control, acetone as a negative control and trigona honey with a concentration of 500 ppm. Antibacterial activity was seen by measuring the inhibition zone formed. The results showed that Trigona honey was able to inhibit the growth of Escherichia coli and Klebsiella pneumoniae bacteria with inhibition zone activity included in the weak category, while the positive control showed antibacterial activity in the strong category.
Profil Pertumbuhan dan Produksi Mutan Bawang Putih (Allium sativum L.) Kultivar Doulu Generasi MV5 Marbun, Jumita Sari; Gultom, Endang Sulistyarini
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 1 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i1.2291

Abstract

Penelitian ini  bertujuan untuk mengetahui pengaruh radiasi sinar gamma terhadap pertumbuhan dan produksi dari tanaman bawang putih (Allium sativum L.) kultivar Doulu pada generasi Mutasi Vegetatif ke 5. Penelitian ini dilakukan selama 4 bulan dari tanggal 3 September 2022 - 2 Januari 2023. Penelitian ini dilaksanakan di Desa Sionggang Utara, Kabupaten Toba Samosir, Sumatera Utara menggunakan 240 sampel siung bawang putih (Allium sativum L.) kultivar Doulu generasi MV4. Rancangan yang digunakan dalam penelitian ini yaitu Rancangan Acak Kelompok (RAK), menggunakan uji AVONA dan memakai uji lanjut BNJ 5%. Dosis perlakuan dari sinar gamma ini adalah G0 (kontrol), G1 (2 Gy), G2 (4 Gy), G3 (6 Gy), G4 (8 Gy), dan G5 (10 Gy). Parameter yang diamati yaitu umur tumbuh, jumlah daun, diameter umbi, berat umbi, jumlah siung, berat siung, dan diameter siung. Susunan daun pada tanaman bawang putih sama yaitu rendah atau low. Struktur umbi bawang putih nya regular two-fan groups, regular multi-cloved radial, regular multi-fan groups, dan irregular. Pertumbuhan bawang putih pada dosis 4 Gy yang lebih baik untuk digunakan. Sedangkan untuk umur tumbuh (4 Gy), jumlah daun (10 Gy), diameter umbi (4 Gy), berat umbi (4 Gy), jumlah siung (8 Gy), berat siung (4 Gy), dan diameter siung (4 Gy). 
IMPLEMENTATION OF LEARNING BASED ON E-WORKSHEETS WITH A SCIENTIFIC APPROACH ON THE MATERIAL OF THE DIGESTIVE SYSTEM IN CLASS XI SMA UNGGULAN AL-MANAR Gultom, Endang Sulistyarini; Yenda, Azura Nur
Jurnal Pelita Pendidikan Vol. 13 No. 1 (2025): JURNAL PELITA PENDIDIKAN
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to determine the application of e-worksheet with a scientific approach in class XI IPA SMA Unggulan Al-Manar in the learning process as measured by students' critical thinking skills. The instrument for measuring the question is administered both before and after the implementation of the learning. The data analysis technique employs quantitative analysis, specifically the calculation of the average value, to assess the students' critical thinking skills. The results of the data analysis indicate that the implementation of learning using e-worksheet with a scientific approach can enhance the critical thinking skills of students in class XI IPA at SMA Unggulan Al-Manar. This is evidenced by the change in the cognitive value of students during the pretest and posttest, which fell within the very good category. The average value of students during the pretest was 54.13, while the average value increased to 86.75 during the posttest, indicating an improvement in critical thinking skills.
Identification of Bacterial Symbiont Sponge with Antibacterial Activity against Multi Drug Resistant Organism (MDRO) Bacteria from Sea Waters in Sibolga, North Sumatra Indonesia Gultom, Endang Sulistyarini; Hasruddin, Hasruddin; Sitompul, Aida Fitriani; Situmorang, Albert D; Prasetya, Eko
Biosfer: Jurnal Tadris Biologi Vol 12 No 2 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i2.10138

Abstract

This research employed the experimental method with descriptive data analysis. It aimed to isolate and identify sponge symbiont bacteria with antibacterial potential against Multi-Drug Resistance Organisms (MDRO) with the 16S rRNA gene marker. Based on the study results, two sponges were obtained, namely Clathrina sp and Agelas sp, each having 11 and 7 isolates of symbiont bacteria, respectively. Based on the antibacterial activity test results, out of 18 isolates of symbiont bacteria, only 4 had antibacterial potential against MDRO (Klebsiella pneumoniae ESBL, Pseudomonas aeruginosa ESBL, or Staphylococcus lugdunensis MRSA) with inhibition zones of 9.3 mm, 8.3 mm, and 8.1 mm, respectively. The results of bacterial identification using the 16S rRNA gene sequence isolate S1I3, S1I9, and A1I3 belong to the Bacillus cereus and Bacillus paramycoides types. This research is expected to provide important information about sponge symbiont bacteria with antibacterial potential against MDRO.
Co-Authors Aida Fitriani Sitompul Eko Prasetya, Eko Habsari, Lilis Hasruddin Jayawarsa, A.A. Ketut Marbun, Jumita Sari Mayasari , Ulfayani Nasution, Mhd Yusuf Pulungan, Ahmad Shafwan S Rangkuti, Marlinda Nilan Sari Rasyidah Rini Hafzari Selvia Dewi Pohan Situmorang, Albert D Situmorang, Nurbaity Yenda, Azura Nur