dela Cruz, Joseph Flores
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Pathogenicity of Clostridium perfringens Philippine Isolate in Necrotic Enteritis Across Broiler Growth Stages Racho, Ma. Rosario S.; Purnamasari, Listya; Olarve, Joseph P.; Rovira, Hope G.; dela Cruz, Joseph Flores
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.124-133

Abstract

Clostridium perfringens was isolated from Philippines broiler chickens of a local farm exhibiting clinical signs of necrotic enteritis. This local isolate induced necrotic enteritis (NE) experimentally in susceptible broiler chickens to demonstrate the disease and the lesions it would produce. Experimental chickens were subjected to stress such as vaccination and pathogenic Escherichia coli and Salmonella enteritidis. Seven treatment groups involving various combinations of the above agents were used to demonstrate lesions of NE. Gross intestinal lesion scoring was performed at necropsy on the 3rd, 7th, 11th, 14th, and 18th-day post-infection, depending on the treatment group. Results showed that the local C. perfringens isolate was able to cause lesions of enteritis but did not demonstrate the classic towel-like lesion of NE, as described by the literature. The treatment combination of C. perfringens and S. enteritidis produced the highest intestinal lesions scores. However, C. perfringens alone can experimentally induce enteritis with a lesser severity. This is the first report of experimental induction of NE in broiler chickens using a local C. perfringens isolate in the Philippines.
Effects of Dietary Plant-Derived Anti-Coccidia on the Production Performance and Oocyst Output of Broiler Chickens Naag, Hazel M.; Purnamasari, Listya; Olarve, Joseph P.; dela Cruz, Joseph Flores
Buletin Peternakan Vol 49, No 2 (2025): BULETIN PETERNAKAN VOL. 49 (2) MAY 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i2.101217

Abstract

Adding feed additives to combat coccidiosis, such as anti-coccidial drugs to broiler diets, can lead to side effects, meat residues, and antimicrobial resistance issues. Alternatively, plant-derived anti-coccidia could serve as safe and simple alternatives compared to synthetic/chemical feed additives. The objective of this study was to evaluate the production performance and oocyst output of broilers fed diets with plant-derived anti coccidia. A total of 300 day-old Cobb 500 broiler chicks were randomly assigned in 30 cages in a completely randomized design with 3 treatment groups, 10 replicates per treatment of 10 chicks per cage. The treatment diets were fed for 35 days: basal diet without anti-coccidia (T1), 500ppm synthetic anti-coccidia containing Narasin and Nicarbazin (T2), and 500ppm plant-derived anti-coccidia containing Cinnamomum camphora, Elephatopus scaber, and Valeriana walichii (T3). Results showed significant differences (p<0.05) in the average body weight gain, feed intake, feed efficiency, and income over feed and chick cost among the three treatment groups. Fecal analysis results showed the absence of oocysts in those feeds with synthetic anti-coccidia and plant derived anti-coccidia. T3 that contains plant-derived anti-coccidia has the highest growth performance among other treatments. In conclusion, plant-derived anti coccidia can be used as an alternative anti-coccidial drug without compromising the growth performance of broiler chickens
Efficacy of Calcium Pidolate Supplementation on the Growth Performance, Tibial Breaking Strength and Income over Feed Cost of Broiler Chickens Aguilon, Loren Domondon; Purnamasari, Listya; Olarve, Joseph Penano; dela Cruz, Joseph Flores
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.7

Abstract

Calcium pidolate is an organic calcium source that can be used as a supplement in the diet of broiler chickens. This is an innovative new form of calcium supplementation that promotes mineral absorption in broiler chickens. This study aimed to determine the effect of calcium pidolate supplementation on the growth performance of broiler chickens. A total of 120 one-day-old Ross chicks were used in this study. The broiler chickens were randomly distributed into two treatment groups with three replicates per treatment. The two treatment groups were the control group with calcium phosphorus supplementation and treatment 2 (T2) with calcium pidolate supplementation. The evaluated growth performance parameters included body weight, body weight gain, average daily gain, average feed consumption, feed efficiency, feed conversion ratio, percent liveability, and tibia breaking strength. The income over feed and chick cost (IOFCC) was also evaluated. The results showed no significant improvement in body weight, body weight gain, average daily gain, average feed consumption, feed efficiency, or percent viability in the T2 group. However, there was a significant increase in the tibia breaking strength of the T2 group compared to that of the control group. In addition, the control group had a greater IOFCC than did the T2 group. In summary, calcium pidolate supplementation may be used as a calcium source for improving the bone strength of broiler chickens.
Evaluation of Adulticidal Efficacy of Cypermethrin, Tetramethrin, and Piperonyl Butoxide in Housefly (Musca Domestica) Licup, Bianca Patricia E.; Purnamasari, Listya; Olarve, Joseph Penano; dela Cruz, Joseph Flores
JURNAL KESEHATAN LINGKUNGAN Vol. 16 No. 3 (2024): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v16i3.2024.222-228

Abstract

Introduction: Houseflies serve as vectors of diseases in animals and humans. Chemical pesticides are a practical way of eliminating insects; however, resistance to insecticides has been reported. This study aims to evaluate the adulticidal efficacy of cypermethrin, tetramethrin, and piperonyl butoxide in housefly. Methods: Adult flies were cultured within net enclosures using completely randomized design with four replications each treatment. Treatment A was water and served as the negative control, treatment B was cypermethrin + tetramethrin + piperonyl butoxide 32% EC and served as the positive control, and treatment C was cypermethrin + tetramethrin + piperonyl butoxide 48% EC. The number of dead houseflies were counted 15, 30, 60, and 120 minutes post- application of treatments. The results were analyzed through analysis of variance (P≤0.05) followed by Tukey’s HSD (honestly significant difference). Results and Discussion: The values for the cumulative mean and percentage of killed houseflies were all significantly different from each other, with treatment C showing the highest values, followed by treatment B, then treatment A. The maximum adulticidal effect of treatment C was apparent within 15 minutes post-application, while the maximum adulticidal effect of treatment B was observed within 30 minutes after application. Conclusion: Results suggest that treatment C was a more effective adulticidal than other groups. Using cypermethrin + tetramethrin + piperonyl butoxide 48% EC can be the solution to the problem, but it should be noted that future resistance may occur. Increased concentrations may also imply environmental, human, and animal health hazards.
Effects of Dietary Inclusion of Angelica gigas Nakai root extract on the Growth Performance, Hematological and Serum Biochemical Parameters in Broilers dela Cruz, Joseph Flores; Purnamasari, listya; Hwang, Seong Gu
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.91533

Abstract

The study aimed to evaluate the impact of dietary supplementation with Angelica gigas Nakai (AGN) root extract on growth performance, hematological indices, and serum biochemical parameters in broiler chickens. A total of 320 straight-run Cobb broiler chicks from a commercial hatchery were distributed among four treatment groups: Basal diet (BD) as the Control; Treatment 1 (T1): BD + 2 g/kg AGN; Treatment 2 (T2): BD + 4 g/kg AGN; and Treatment 3 (T3): BD + 8 g/kg AGN), each comprising eight replicates with 10 birds per replicate. The supplementation of AGN resulted in dose-dependent improvements (P < 0.05) in body weight, gain, and feed efficiency. On both day 21 and day 35, increasing AGN dosage in the diet led to a significantly higher (P < 0.05) values of red blood cells (RBCs), white blood cells (WBCs), hemoglobin (Hb), and packed cell volume (PCV). By day 21, AGN supplementation dose-dependently decreased (P < 0.05) serum alkaline phosphatase (ALP), aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), urea, and creatinine levels. Meanwhile, AGN dose escalation correlated with a notable increase (P < 0.05) in serum total protein (TP), albumin, and globulin levels. On day 35, increasing AGN levels led to a significant reduction (P < 0.05) in serum AST and ALT activity, along with lowered serum glucose, cholesterol, triglycerides, urea, and creatinine levels. In conclusion, AGN supplementation enhanced growth performance and positively influenced hematological indices and serum biochemistry profiles in broiler chickens. The study confirms the safe and effective utilization of AGN at an 8 g/kg (0.8 %) feed additive dosage to optimize broiler performance. These findings provide valuable insights into the potential benefits of AGN in poultry nutrition.