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Determination of Reducing Sugar Groups from Hydrolysis of Arthrospira platensis Microalgae using Microwaves Ma'mun, Sholeh; Rochmaningsih, Nur Ariffa; Nabila, Riqqah; Sumalyani, Aisyah
BERKALA SAINSTEK Vol 12 No 2 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i2.47304

Abstract

The depletion of fossil fuel sources and increasing carbon dioxide (CO2) emissions have encouraged research into renewable energy sources. Bioethanol is an environmentally friendly energy source with considerable potential for reducing dependence on gasoline. Bioethanol is produced from the fermentation process of monosaccharides. The first and second generations of bioethanol are derived from food crops, agricultural waste, and plantation waste, whereas the third generation is produced from microalgae. Arthrospira platensis is a carbohydrate-rich microalgae. This study attempts to determine the content of reducing sugar groups which are monosaccharides formed from a hydrolysis with microwaves. A total of 10 g of microalgae powder was added to 100 mL of 0.3 M H2SO4 solution. The hydrolysis process was carried out in a microwave reactor at a temperature of 100°C for 90 minutes. The hydrolysate obtained was then fermented with Saccharomyces cerevisiae anaerobically in a shaking water bath. The High Performance Liquid Chromatography (HPLC) test was performed to identify reducing sugar groups in the hydrolysate, and the Gas Chromatography (GC) test was performed to determine the concentration of bioethanol produced during the fermentation process. Meanwhile, the solid content of biochar that remained after hydrolysis was analyzed using Fourier-Transform Infrared Spectoscopy (FTIR). The HPLC test findings showed that the glucose concentrations before and after fermentation were 10.52 and 1.91 g/L, respectively, indicating that 81.8% of the glucose was converted to bioethanol. Furthermore, the distillation results from the fermented hydrolysate were analyzed using GC, yielding a bioethanol content of 3.90 g/L.
Zodia (Evodia suaveolens) Leaves as Natural Mosquito Repellent Material: Effectiveness Test of Distillation Methods Sumalyani, Aisyah; Ma'mun, Sholeh; Riyanto, Riyanto
BERKALA SAINSTEK Vol. 12 No. 4 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i4.53351

Abstract

Indonesia has around 40 types of plants with essential oil content from a total of around 150 types of essential oils traded in the international market. Essential oils are produced from various parts of aromatic plants such as fruits, seeds, leaves, flowers, roots, stems, bark, and even the entire tree. One of the plants that contains and has the potential to produce essential oils is the zodia (Evodia suaveolens), especially the leaves. Evodone is one of the active compounds contained in the essential oil of zodia leaves which has the ability as a mosquito repellent. This study aims to determine the effectiveness of hydrodistillation, steam-hydrodistillation, and steam distillation in terms of yield, physical properties, and active insecticide compound content. A total of 1000 g of fresh zodia leaves were put into a distillation kettle either boiled, steamed, or in direct contact with steam for three hours at 100 °C and atmospheric pressure. The remaining water content in the essential oil from the distillate was then absorbed with Na2SO4. Furthermore, the water-free essential oil was tested for its physical properties consisting of density, optical rotation, refractive index, and color tests, and then its chemical composition was analyzed using GC-MS. The results showed that the yield for hydrodistillation, steam-hydrodistillation, and steam distillation were 0.37%; 0.43%; and 0.31%, respectively. From the optical rotation test, the essential oil from steam distillation had the lowest value indicating the highest level of purity compared to the other two methods. This is in accordance with the density and refractive index data, where the essential oil from steam distillation had the highest value, where the color was darker. Meanwhile, based on the results of GC-MS analysis, the highest active insecticide compound in the form of evodone was obtained from steam-hydrodistillation (14.25%), followed by hydrodistillation (7.62%), and steam distillation (4.81%).