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Bioethanol Production from Spirulina (Arthrospira platensis) by Microwave-Assisted Acid Hydrolysis Pretreatment Ma'mun, Sholeh; Prasetio, Muhammad Wisnu; Anugrah, Aditya Rizki
Jurnal Bahan Alam Terbarukan Vol 13, No 1 (2024): June 2024 [Nationally Accredited Sinta 2]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v13i1.49344

Abstract

The world's high consumption of fossil energy increases carbon dioxide (CO2) emissions. The depletion of fossil fuel sources, combined with rising CO2 emissions, has prompted intensive research into renewable energy sources. Bioethanol is an environmentally friendly energy source that has the potential to reduce reliance on gasoline. Bioethanol is produced through the fermentation of monosaccharides. The first and second generations of bioethanol are derived, respectively, from food crops, agricultural waste, and plantations, while the third generation is derived from algae. However, the third generation bioethanol research is still being conducted intensively to develop an optimal process. Macro/microalgae are low-level plants that have the potential to become raw materials for bioethanol. Arthrospira platensis, a spirulina species, is a microalgae with a high carbohydrate content. Apart from that, this type of microalgae is easy to cultivate and grow. This research aims to determine the reducing sugar content which are monosaccharides produced from acid hydrolysis using a microwave at a temperature of 100 °C for 60 – 120 minutes with 0.2 M H2SO4 as the solvent. The hydrolysate obtained was then fermented anaerobically with Saccharomyces cerevisiae in a shaking water bath. The High Performance Liquid Chromatography (HPLC) test was carried out to identify the reducing sugar groups in the hydrolysate. Moreover, the solid content of the biochar remaining from the hydrolysis process was analyzed using Fourier-Transform Infrared Spectrometer (FTIR). From the test results, it was found that the highest concentration of D-glucose (1.19 g/L) occurred at 90 minutes of the hydrolysis. In addition, the hydrolysis of microalgae was also carried out with 0.3 M H2SO4 solvent for 90 minutes. The hydrolysate was then fermented for 96 hours. From the distillation process, it was obtained a bioethanol yield of 2.89%.
Identifikasi Gugus Gula Pereduksi Mikroalga Arthrospira platensis Hasil Hidrolisis dengan Bantuan Gelombang Mikro Ma'mun, Sholeh
Jurnal Teknik Vol 13, No 1 (2024): Januari - Juli 2024
Publisher : Universitas Muhammadiyah Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31000/jt.v13i1.10826

Abstract

Menipisnya sumber bahan bakar fosil dan meningkatnya emisi karbon dioksida (CO2) telah mendorong kegiatan penelitian untuk menemukan sumber-sumber energi terbarukan. Bioetanol merupakan sumber energi yang ramah lingkungan dan memiliki prospek yang menjanjikan untuk mengurangi ketergantungan pada gasolin. Bioetanol dihasilkan dari proses fermentasi monosakarida. Bioetanol generasi pertama dan kedua berasal dari tanaman pangan, limbah pertanian, dan limbah perkebunan, sedangkan generasi ketiga dihasilkan dari mikroalga. Arthrospira platensis merupakan salah satu jenis mikroalga dengan kandungan karbohidrat yang tinggi. Penelitian ini bertujuan untuk menentukan kandungan gugus gula pereduksi yang merupakan monosakarida hasil hidrolisis dengan bantuan gelombang mikro. Sebanyak 10 g serbuk mikroalga ditambahkan ke dalam 100 mL larutan 0,3 M H2SO4. Proses hidrolisis dijalankan dalam sebuah reaktor microwave pada suhu 100 °C selama 90 menit. Selanjutnya hidrolisat yang diperoleh difermentasi dengan ragi Saccharomyces cerevisiae secara anaerob di dalam shaking water bath. Uji HPLC dilakukan untuk mengidentifikasi gugus-gugus gula pereduksi dalam hidrolisat, sedangkan uji GC dilakukan untuk menentukan konsentrasi bioetanol yang dihasilkan dari proses fermentasi. Sementara itu, kandungan padatan biochar sisa hidrolisis dianalisis menggunakan FTIR. Dari hasil pengujian HPLC diperoleh konsentrasi glukosa sebelum fermentasi sebesar 11,963 g/L dan setelah fermentasi sebesar 0,546 g/L atau sebanyak 95% glukosa terkonversi menjadi etanol. Selanjutnya hasil distilasi dari fermentasi hidrolisat diuji dengan GC dan diperoleh kadar etanol sebesar 0,39%.
An Investigation into the Effectiveness of Green Betel (Piper betle L.) Leaf Extract Hand Sanitizer Yahya, Diyas Aledya; Permatasari, Intan; Ma'mun, Sholeh
BERKALA SAINSTEK Vol 11 No 2 (2023)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v11i2.39190

Abstract

Green betel (Piper betle L.) leaf contains anti-thrush, anti-cough, astringent, and antiseptic chemicals such as saponins, flavonoids, polyphenols, and essential oils. Hand sanitizer can also be used as an antiseptic agent, which is a more practical option. The purpose of this study was to see how the composition of green betel leaf extract made by infusion affected its physical properties and bacterial inhibition. Hand sanitizer samples were made using different concentrations of green betel leaf extract (10 - 25 wt%) with and without the addition of tea tree essential oil. Sample testing included organoleptic tests, physical properties tests, and effectiveness tests on the growth of Escherichia coli bacteria. The results showed that the best composition was found to be at a concentration of 10 wt% green betel leaf extract without the addition of essential oil, with pH ranging from 5.2 to 5.8 and viscosities ranging from 1.32 to 1.99 cps, in the form of a watery gel and a clear yellow color. Meanwhile, hand sanitizer sensitivity testing revealed that none of the samples could inhibit the growth of Escherichia coli bacteria. This indicates that the concentration of green betel leaf extract in the sample is still insufficient to inhibit bacterial growth.
Determination of Reducing Sugar Groups from Hydrolysis of Arthrospira platensis Microalgae using Microwaves Ma'mun, Sholeh; Rochmaningsih, Nur Ariffa; Nabila, Riqqah; Sumalyani, Aisyah
BERKALA SAINSTEK Vol 12 No 2 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i2.47304

Abstract

The depletion of fossil fuel sources and increasing carbon dioxide (CO2) emissions have encouraged research into renewable energy sources. Bioethanol is an environmentally friendly energy source with considerable potential for reducing dependence on gasoline. Bioethanol is produced from the fermentation process of monosaccharides. The first and second generations of bioethanol are derived from food crops, agricultural waste, and plantation waste, whereas the third generation is produced from microalgae. Arthrospira platensis is a carbohydrate-rich microalgae. This study attempts to determine the content of reducing sugar groups which are monosaccharides formed from a hydrolysis with microwaves. A total of 10 g of microalgae powder was added to 100 mL of 0.3 M H2SO4 solution. The hydrolysis process was carried out in a microwave reactor at a temperature of 100°C for 90 minutes. The hydrolysate obtained was then fermented with Saccharomyces cerevisiae anaerobically in a shaking water bath. The High Performance Liquid Chromatography (HPLC) test was performed to identify reducing sugar groups in the hydrolysate, and the Gas Chromatography (GC) test was performed to determine the concentration of bioethanol produced during the fermentation process. Meanwhile, the solid content of biochar that remained after hydrolysis was analyzed using Fourier-Transform Infrared Spectoscopy (FTIR). The HPLC test findings showed that the glucose concentrations before and after fermentation were 10.52 and 1.91 g/L, respectively, indicating that 81.8% of the glucose was converted to bioethanol. Furthermore, the distillation results from the fermented hydrolysate were analyzed using GC, yielding a bioethanol content of 3.90 g/L.
Zodia (Evodia suaveolens) Leaves as Natural Mosquito Repellent Material: Effectiveness Test of Distillation Methods Sumalyani, Aisyah; Ma'mun, Sholeh; Riyanto, Riyanto
BERKALA SAINSTEK Vol. 12 No. 4 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i4.53351

Abstract

Indonesia has around 40 types of plants with essential oil content from a total of around 150 types of essential oils traded in the international market. Essential oils are produced from various parts of aromatic plants such as fruits, seeds, leaves, flowers, roots, stems, bark, and even the entire tree. One of the plants that contains and has the potential to produce essential oils is the zodia (Evodia suaveolens), especially the leaves. Evodone is one of the active compounds contained in the essential oil of zodia leaves which has the ability as a mosquito repellent. This study aims to determine the effectiveness of hydrodistillation, steam-hydrodistillation, and steam distillation in terms of yield, physical properties, and active insecticide compound content. A total of 1000 g of fresh zodia leaves were put into a distillation kettle either boiled, steamed, or in direct contact with steam for three hours at 100 °C and atmospheric pressure. The remaining water content in the essential oil from the distillate was then absorbed with Na2SO4. Furthermore, the water-free essential oil was tested for its physical properties consisting of density, optical rotation, refractive index, and color tests, and then its chemical composition was analyzed using GC-MS. The results showed that the yield for hydrodistillation, steam-hydrodistillation, and steam distillation were 0.37%; 0.43%; and 0.31%, respectively. From the optical rotation test, the essential oil from steam distillation had the lowest value indicating the highest level of purity compared to the other two methods. This is in accordance with the density and refractive index data, where the essential oil from steam distillation had the highest value, where the color was darker. Meanwhile, based on the results of GC-MS analysis, the highest active insecticide compound in the form of evodone was obtained from steam-hydrodistillation (14.25%), followed by hydrodistillation (7.62%), and steam distillation (4.81%).
Lauryl Hydroxysultaine as a Low Dose Foam Enhancing Booster Putri, Avicenna Mustika; Ma'mun, Sholeh; Zulkania, Ariany
BERKALA SAINSTEK Vol. 12 No. 4 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i4.53374

Abstract

The growth of Indonesia's cosmetics industry reached 21.9%, with 913 companies in 2022 and 1,010 companies by mid-2023. Among the total products from local cosmetic companies, the largest market segment is personal care. Shampoo and bodywash are the two main categories of personal care cleansing products. Foam is an important indicator of product quality; however, technically, foam does not always correlate with the cleaning strength of a product. Nevertheless, consumers expect shampoos and body washes to lather easily and produce abundant foam. The foaming ability of a product is influenced by several factors, one of which is the addition of foam boosters. Cocamidopropyl betaine (CAPB) is the most used foam booster and is produced on a large scale. Another foam booster is lauryl hydroxysultaine (LHS) which has better foam-producing ability than CAPB. This study aims to determine the right formulation of LHS addition in shampoo and bodywash thus it meets the specified requirements. Shampoo and bodywash were prepared with several formulations, the samples were then tested for their viscosity, foam quality, and irritation potential. The results showed that LHS performed better than CAPB for the three parameters above. It was obtained that a dose of 2% LHS gave a better effect than 3% CAPB. It is, therefore, quite profitable from an economic and environmental perspective.