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All Journal Bioscience Jurnal Serambi Biologi BIO-SAINS | Jurnal Ilmiah Biologi
Sirwati, Fadila
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Comparison of Boiling Methods for Bacterial DNA Isolation Using Waterbath and Heatblock Sirwati, Fadila; Nurfalinda, Siti; Salsabila, Siti; Putri, Dwi
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.328

Abstract

Molecular biology analysis has several advantages over culture approaches, including the ability to identify a wider range of target organisms, increased sensitivity and specificity. Bacterial DNA isolation is a simple solution in molecular biology analysis. Boiling techniques using high temperature heating can damage cell wall permeability. Heating boiling technique can be done by using waterbath, and heat block with different heat transfer mechanism. The results showed the concentration value of samples isolated using headblock was in the range of 77.6 ug/ul - 200.45 ug/ul, while the concentration of samples isolated using waterbath was in the range of 145.575 ug/ul - 288.8 ug/ul. The highest DNA concentration was obtained from using a waterbath. Purity values measured at wavelengths A260/A280 were obtained with a range of purity that was not different.
Genetic Variation Analysis of katG Gene Sequence in Mycobacterium tuberculosis Using RFLP In Silico Nurfalinda, Siti; Aulia, Ony Nattasha; Sirwati, Fadila; Alridho, Rezeki Rival; Achayar, Afifatul
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.337

Abstract

Tuberculosis (TB) is an infectious disease that is the leading cause of death in Indonesia. This infection is caused by Mycobacterium tuberculosis. In Indonesia in 2021, there were approximately 824,000 cases of TB. A total of 8,268 TB cases were associated with resistance of M. tuberculosis to anti-tuberculosis drugs (OAT). MDR-TB is a strain that is resistant to two of the most important anti-tuberculosis drugs in first-line TB therapy, namely Rifampicin (RIF) and Isoniazid (INH). The most common mechanism of INH resistance is the katG gene mutation, found (95%) at codon 315. In the katG gene there is a change in the amino acid Ser (AGC) → Thr (ACC). Genetic variation in a population will affect the survival ability of an individual. This study used BamHI restriction enzyme. This study aims to analyze genetic variation in the KatG gene sequence in M. tuberculosis NCBI popset 2363230696 by using RFLP in silico. The results showed that there were genetic variations in the KatG gene sequence and two allelic variations contained in 7 KatG gene sequences using the BamHI restriction enzyme. Key words : M. tuberculosis, BamHI, KatG , Genetic Variation, RFLP in silico.
Literature Review: Benefits and applications of alginate liase enzyme) Sirwati, Fadila; Putri, Dwi Hilda; Rachmayati, Rike
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.131077

Abstract

Alginate is a polymer found in large amounts in the cell walls of brown algae. This polymer consists of α-L guluronic acid (G) and mannuronic acid (M). Alginate can be degraded by an enzyme, known as alginate liase by removing the glycosidic bond and producing an unsaturated oligosaccharide with a double bond at the non-reducing end. To provide an overview of the utilization of alginate lyase, information is needed on the biological activity of alginate lyase as an antibiofilm agent, the production of alginate oligosaccharides, and its antioxidant properties. This study aims to provide scientific insight into the important role of the benefits and applications of alginate lyase. The research design used was a literature review. Articles were collected based on sources from PubMed, Science Direct, and Google Scholar, which included more than thirty national and international journals. The articles collected showed that alginate lyase exhibits many biological activities, including antibiofilm, antioxidant, and oligosaccharide production. These properties have the potential to be used in a wide range of applications, which include the food, pharmaceutical, and biotechnology industries.
Isolasi Jamur Capnodium sp. Penyebab Penyakit Embun Jelaga pada Tanaman Jambu Air (Syzygium aqueum) Sirwati, Fadila
BIO-SAINS : Jurnal Ilmiah Biologi Vol 4 No 1 (2024): Bio-Sains Vol 4 No 1, Maret 2024
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/bio-sains.v3i2.3551

Abstract

Jambu air atau dalam bahasa ilmiah Syzygium aqueum termasuk ke dalam Famili Myrtaceae yang banyak dibudidayakan di Indonesia. Salah satu permasalahan dalam membudidayakan jambu air adalah daunnya yang rentan diserang penyakit embun jelaga yang disebabkan kutu. Penyakit embun jelaga disebabkan oleh jamur Capnodium sp. yang memiliki miselium berwarna hitam dan menutupi permukaan daun jambu air. Penelitian ini termasuk ke dalam penelitian deskriptif, dengan melakukan pengisolasian embun jelaga pada daun S. aqueum. Pengamatan penelitian meliputi karakteristik morfologi jamur Capnodium sp. secara makroskopis dan mikroskopis. Pengamatan secara makroskopis memperlihatkan warna permukaan Capnodium sp. berwarna hitam pada bagian tengah dan putih pada bagian tepi, warna dasar hitam, dengan tekstur permukaan kasar dan seperti kapas, koloni berbentuk bulat, tepi tidak rata dengan diameter 6 cm. Pengamatan secara mikroskopis memperlihatkan hifa bersekat dan berwarna gelap. Capnodium sp. memiliki hifa berbentuk silinder dan percabangan polipodial. Terdapat konidia berbentuk oval sebagai reproduksi aseksual, dan perithecium sebagai reproduksi seksual.
Co-Authors Achayar, Afifatul Alridho, Rezeki Rival Aulia, Ony Nattasha Dwi Hilda Putri Dwi Putri, Dwi Nurfalinda, Siti Rachmayati, Rike Salsabila, Siti