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All Journal Scientific Journal of Dermatology and Venereology Sriwijaya Journal of Forensic and Medicolegal Crown: Journal of Dentistry and Health Research
Rinna Azrida
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Public Health

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Molecular Epidemiology of Tinea Pedis Among Palm Oil Plantation Workers in Labuhan Batu Utara Regency, Indonesia: Identification of Causative Fungal Pathogens Haryani, Sela; Riri Arisanty Syafrin Lubis; Rinna Azrida
Scientific Journal of Dermatology and Venereology Vol. 1 No. 2 (2023): Scientific Journal of Dermatology and Venereology
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/sjdv.v1i1.5

Abstract

Introduction: Tinea pedis, commonly known as athlete's foot, is a prevalent fungal infection affecting the feet. Palm oil plantation workers, often exposed to humid and warm conditions, are at an increased risk of contracting this condition. This study aimed to investigate the molecular epidemiology of tinea pedis among palm oil plantation workers in Labuhan Batu Utara Regency, Indonesia, and identify the causative fungal pathogens. Methods: A cross-sectional study was conducted among palm oil plantation workers in Labuhan Batu Utara Regency. Demographic data and clinical information were collected through questionnaires. Skin scrapings were obtained from the affected areas of the feet and subjected to microscopic examination and fungal culture. Molecular identification of the isolated fungi was performed using DNA sequencing of the internal transcribed spacer (ITS) region. Results: A total of 250 palm oil plantation workers participated in the study. The prevalence of tinea pedis was found to be 42.8%. The most common clinical presentations were interdigital scaling and maceration (65.2%), followed by vesicular lesions (23.1%) and hyperkeratosis (11.7%). The predominant fungal pathogens identified were Trichophyton rubrum (58.3%), Trichophyton mentagrophytes (27.8%), and Epidermophyton floccosum (13.9%). Conclusion: Tinea pedis is a significant health problem among palm oil plantation workers in Labuhan Batu Utara Regency. The identification of the causative fungal pathogens is crucial for implementing effective preventive and therapeutic measures to control the spread of this infection.
Factors Affecting the Incidence of Tinea Pedis in Palm Oil Plantation Workers in Sonomartani Village, Kualuh Hulu District, Labuhan Batu Utara Regency, Indonesia Haryani, Sela; Riri Arisanty Syafrin Lubis; Rinna Azrida
Scientific Journal of Dermatology and Venereology Vol. 1 No. 1 (2023): Scientific Journal of Dermatology and Venereology
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/sjdv.v1i1.194

Abstract

by humid conditions, greatly affects the high incidence of tinea pedis in oil palm workers. This cannot be separated from minimal knowledge about the importance of keeping feet clean while working. This study aimed to determine the factors that influence the incidence of tinea pedis in oil palm plantation workers in Sonomartani Village, Kualuh Hulu District, Labuhan Batu Utara Regency, Indonesia. Methods: Cross-sectional observational study. A total of 48 research subjects participated in this study. Observations on sociodemographics and factors of the risk of developing tinea pedis were carried out in this study. Data analysis was carried out using SPSS univariate and bivariate. Results: The results of the study showed that age <35 years and female gender were associated with the incidence of tinea pedis, p<0.05. Personal hygiene factors such as using their own towels, washing feet after activities, and the habit of drying shoes after use are associated with the incidence of tinea pedis, p<0.05. Conclusion: The habit of washing feet and keeping shoes dry is a factor risk that contributes to the incidence of tinea pedis in oil palm plantation workers in Sonomartani Village, Kualuh Hulu District, Labuhan Batu Utara Regency, Indonesia.
Forensic DNA Phenotyping for Predicting Externally Visible Characteristics in Indonesian Populations: A Novel Tool for Criminal Investigations Rinna Azrida; Febria Suryani; Bjorka Alma; Sony Sanjaya; Khairiel Anwar
Sriwijaya Journal of Forensic and Medicolegal Vol. 2 No. 2 (2024): Sriwijaya Journal of Forensic and Medicolegal
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/sjfm.v2i2.190

Abstract

Introduction: Forensic DNA phenotyping (FDP) is an emerging field that utilizes genetic information to predict an individual's externally visible characteristics (EVCs). While its application has shown promise in aiding criminal investigations globally, its utility within the diverse Indonesian population remains largely unexplored. This study aimed to investigate the potential of FDP for predicting EVCs in a representative sample of the Indonesian population, evaluating its accuracy and implications as a novel tool for criminal investigations in this unique context. Methods: This study involved the analysis of anonymized DNA samples from 1000 individuals self-identified as belonging to various ethnic groups across Indonesia. A panel of single nucleotide polymorphisms (SNPs) known to be associated with EVCs including hair color, eye color, skin pigmentation, and biogeographic ancestry (BGA) was selected based on existing literature. Genotyping data was generated to reflect the genetic diversity observed in Indonesian populations. Statistical analyses were performed to assess the predictive power of the SNP panel for each EVC and to evaluate the correlation between predicted phenotypes and self-reported characteristics. Results: The results demonstrated a moderate to high predictive accuracy for several EVCs within the Indonesian population. Skin pigmentation showed the highest predictability, followed by eye color and hair color. BGA prediction effectively clustered individuals based on their genetic profiles, aligning with the known population structure of Indonesia. Significant variations in allele frequencies for EVC-associated SNPs were observed across different ethnic groups, highlighting the importance of population-specific data. Conclusion: This study provides preliminary evidence suggesting that forensic DNA phenotyping holds significant potential as a novel tool for criminal investigations in Indonesia. The ability to predict EVCs from DNA could provide valuable leads in cases where traditional DNA profiling yields no matches. However, further research with real Indonesian population data is crucial to validate these findings and to develop robust, population-specific FDP models. Ethical and legal considerations surrounding the use of this technology in the Indonesian context must also be carefully addressed.
In Vitro and In Vivo Efficacy of a Novel Strontium-Doped Bioactive Glass Hydrogel for Dentin-Pulp Complex Regeneration Rinna Azrida; Bryan Helsey; Bernadette Wilson; Mohammad Yoshandi
Crown: Journal of Dentistry and Health Research Vol. 3 No. 1 (2025): Crown: Journal of Dentistry and Health Research
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/crown.v3i1.236

Abstract

Introduction: The regeneration of the dentin-pulp complex is a major challenge in vital pulp therapy. This study aimed to develop and evaluate a novel injectable hydrogel composed of strontium-doped bioactive glass (Sr-BG) in a methacrylated gelatin (GelMA) matrix to promote dentin-pulp complex regeneration. Strontium was added for its dual therapeutic effects of enhancing odontogenic differentiation and inhibiting bacterial activity. Methods: We synthesized Sr-BG nanoparticles using a sol-gel method and characterized them with X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The nanoparticles were then incorporated into a GelMA hydrogel. We assessed the material's physical properties, including its swelling ratio, degradation rate, and ion release profiles (Si, Ca, P, Sr). We also evaluated its in vitro biocompatibility and odontogenic potential using human dental pulp stem cells (hDPSCs), assessing cell viability (MTT assay), alkaline phosphatase (ALP) activity, and the expression of odontogenic markers (DSPP, DMP-1, RUNX2) via RT-qPCR. We tested its antibacterial properties against Streptococcus mutans. For the in vivo evaluation, the hydrogel was used as a pulp capping agent in the mechanically exposed molars of Wistar rats. After 4 and 8 weeks, we assessed tissue regeneration using histological analysis (H&E and Masson's trichrome staining) and micro-computed tomography (micro-CT). Results: The synthesized Sr-BG nanoparticles were amorphous with a particle size of about 80-120 nm. The Sr-BG/GelMA hydrogel exhibited controlled swelling and degradation, along with a sustained release of therapeutic ions. In vitro, the hydrogel demonstrated excellent biocompatibility and significantly upregulated ALP activity and the expression of DSPP, DMP-1, and RUNX2 in hDPSCs compared to the control group (p < 0.05). The material also showed significant antibacterial activity against S. mutans. In vivo, histological analysis revealed the formation of a thick, continuous, and well-organized tertiary dentin bridge with minimal inflammation in the Sr-BG/GelMA group at 8 weeks. Micro-CT analysis confirmed a significantly greater volume and density of newly formed mineralized tissue compared to control groups treated with calcium hydroxide. Conclusion: The novel strontium-doped bioactive glass hydrogel showed significant potential for dentin-pulp complex regeneration. Its combined osteoinductive, angiogenic, and antibacterial properties make it a promising biomaterial for advanced vital pulp therapy, offering a superior alternative to traditional pulp capping agents.
Co-Authors Bernadette Wilson Bjorka Alma Bryan Helsey Febria Suryani HARYANI, SELA Khairiel Anwar Mohammad Yoshandi Riri Arisanty Syafrin Lubis Sony Sanjaya