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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Mufadilah, Mitha Aprilia
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Economics, Econometrics & Finance

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Salt and Heat Stress Trigger Morpho-Physiological Changes, Antioxidant Enzyme and Secondary Metabolites Gene Expression in Rice (Oryza sativa L.) Ubaidillah, Mohammad; Rozzita, Noor; Mufadilah, Mitha Aprilia; Thamrin , Nurhaliza; Puspito, Agung Nugroho; Kim, Kyung Min
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.256-270

Abstract

Climate change significantly increases salt and heat stress in rice plants. This condition causes plants to activate antioxidant enzymes and produce secondary metabolites. This study aimed to determine the morpho-physiological changes and gene expression profiles of antioxidant enzymes and secondary metabolites. This study used a completely randomized design factorial. The first factor was local rice varieties (IR64, Silaun, and Cigeulis), and the second factor was stress treatments (control, NaCl 150 mM, 40°C, and NaCl 150 mM + 40°C). The results showed that multiple stress significantly affected the plant height, stem length, stem diameter, leaf area, root length, total main root, plant biomass, necrotic length, chlorophyll content, relative water content, and plant ROS production. Multiple stress could up-regulate the gene expression of antioxidant enzymes (Mn-SOD, Cu/Zn SOD, Cytosolic APX, OsAPX1, CAT, OsCATA, and GPOD) in rice after stress combination treatments and increase the secondary metabolites gene expression (P5CS and GABA-T) in all rice varieties. Still, the OsNOMT gene was only active in the Cigeulis variety.
The Study of Exogenous Auxin and Cytokinins in Embryogenesis and Fiber Genes Expression during In Vitro Regeneration of Cotton (Gossypium hirsutum L.) Mufadilah, Mitha Aprilia; Thamrin , Nurhaliza; Puspito, Agung Nugroho; Ubaidillah, Mohammad
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.517-529

Abstract

The efficiency of regeneration media, based on the morpho-cytochemical response and embryogenesis gene expression of widely cultivated cotton in Indonesia (Gossypium hirsutum L.), needs to be studied further. This study's objectives are to identify the effect of 2,4-D and IBA hormones on the morphology and induction of cotton callus; to determine the effect of regeneration media on the morphology, cytochemical, and gene expression of cotton plant during embryogenesis. This study was conducted by inducing cotton callus on MS0 medium, MS Basal + 0.1 mg/L 2,4-D and MS Basal + 0.1 mg/L IBA. The best callus induction results were regenerated on the regeneration media, namely M1 (MS Vitamin + 0.01 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), M2 (MS Vitamin + 0.01 ppm 2,4-D + 0.5 ppm IBA + 0.5 ppm Kinetin), M3 (MS Vitamin + 0.00 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), and M4 (MS Vitamin + 0, 00 ppm 2,4- D + 0.5 ppm IBA + 0.5 ppm Kinetin). The embryogenesis gene expression was observed. The best morphological results for callus induction were obtained on MS Basal + 0.1 mg/L 2,4-D medium with a compact and white callus, indicates high embryogenic and regeneration ability. The highest percentage of regenerated callus parameters was found in M2 media and further confirmed by cytochemical characterization. Embryogenesis genes expressed were GhSERK1, GhSERK2, LEC, GhWUS, and GhWOX11. Other fiber-related genes GhMYB25-like, GhHD-1, and F3H were also expressed during the embryogenesis.
Flower bagging affects seed quality and dormancy period in Na-Oogst tobacco (Nicotiana tabacum L. var H-382) Thamrin, Nurhaliza; Amilin, Miftahul; Mufadilah, Mitha Aprilia; Ubaidillah, Mohammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 2 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/ija.v51i2.45493

Abstract

Seeds of tobacco plants are unable to germinate immediately after harvest because they have a dormancy period. The seed dormancy period may be shortened by bagging methods to affect the hormone content in the seeds. The bagging flower makes the dark conditions in the process of flowering and seed development, affecting the content of the hormone’s auxin and ABA. This study aimed to determine the effects of different bag colors on the dormancy period of tobacco seeds as evidenced by their vigor and viability. The research was conducted from March to August 2022 at PT. Dwi Light Tobacco Green House, Lombok Wetan, Bondowoso, Indonesia. Four crepe colors of paper were used as bagging treatments (black, red, yellow, blue) and control (without bagging) and replicated four times. The results showed that the bagging treatment significantly affected one hundred-seed weight, seed germination, seed growth rate, and seedling height. Black crepe paper resulted in the highest seed germination percentage (98.5%) and seed growth rate (11.68). Keywords: bag color, seed germination, viability, vigor
Co-Authors Amilin, Miftahul Kim, Kyung Min Puspito, Agung Nugroho Rozzita, Noor Thamrin , Nurhaliza Thamrin, Nurhaliza Ubaidillah, Mohammad