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All Journal HAYATI Journal of Biosciences Biology, Medicine, & Natural Product Chemistry Jurnal Sumberdaya Hayati Jurnal Biodjati Jurnal Sains Agro Jurnal Pengabdian Masyarakat Majalah Pengabdian Indonesia
Muhamad Azwar Syah
Department Of Biotechnology, Faculty Of Mathematics And Natural Science, Halu Oleo University, Kendari, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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 1 
Cloning and Co-Expression of Lipase and its Specific Foldase from Ralstonia pickettii BK6 Muhamad Azwar Syah; Andreas Adhi Satya; Esti Puspitasari; Antonius Suwanto; Nisa Rachmania Mubarik
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.71-80

Abstract

This study aimed to obtain a functional lipase (LipRM) from Ralstonia pickettii BK6 through a co-expression involving its foldase. The ORF of the LipRM was 999 bp while the gene encoding of lipase-specific foldase (LifRM) was 1,030 bp. LipRM and LifRM genes were cloned into a plasmid and were successfully co-expressed in Escherichia coli strains to produce functional LipRM. Enzyme activity from partially purified enzymes showed quite surprising results, LipRM activity in E. coli BL21 (DE3) was 25.84 U/ml, while the other strains (DH5α, HB101, S17-1λpir) were 628.98 U/ml, 761 U/ml, and 1206.46 U/ml, respectively. The highest relative activity of LipRM was found at 50-55°C and pH 7-8 with pNP-laurate (C12) as the preferred substrate specificity. LipRM activity was enhanced sharply in the presence of 30% organic solvents (methanol and ethanol) but decreased by more than 50% in the presence of detergents. This study was the first to report heterologous expression of Ralstonia pickettii lipase employing its native foldase resulting in functional lipase from subfamily I.2.
Isolasi dan Karakterisasi Molekuler Gen 16S rRNA Bakteri Lipolitik Asal Limbah Kulit Biji Jambu Mete: Isolation and Molecular Characterization of Lipolytic Bacterial 16S rRNA Gene from Cashew Nutshell Waste Muhamad Azwar Syah
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.20-26

Abstract

Lipolytic bacteria attract great attention to various biotechnology industries because of their enzymatic potential. This study aims to isolate and identify lipolytic bacteria from cashew nutshell waste using the 16S rRNA gene as a molecular marker. Lipolytic bacteria were isolated using serial dilutions and inoculated on lipolytic media. A total of 3 isolates of lipolytic bacteria were obtained from cashew nutshell waste based on screening in LA Rhodamine B. The partial sequence of 16S rRNA gene from LB15 amplified using a pair of primers 63F and 1387R having a size of 1238 bp, while BL6 and BK6 were 1283 bp, respectively. Based on genetic distance analysis and phylogenetic reconstruction, we proposed that LB15 be identified as Burkholderia sp. with 99.92% similarity. In addition, because the 16S rRNA gene sequence similarity of BL6 was 99.87% with Paraburkholderia kururiensis strain 979, BL6 was classified as Paraburkholderia kururiensis. Then, isolate BK6 was identified as Ralstonia sp. with a similarity of 99.53%. The similarity value can be used as a reference in determining the identity of bacteria. A bacterium can be categorized as the same species if it has a similarity value of more than 99%.
Training on Processing Natural Resources into Processed Food and Health Products for Mothers in Tondongggeu Village, Nambo District, Kendari City Ambardini, Sri; Ahmad, Sitti Wirdhana; Muhsin, Muhsin; Karya, Adi; Syah, Muhamad Azwar; Adi Parman Rudia, La Ode; Yanti, Nur Arfa
Majalah Pengabdian Indonesia Vol. 1 No. 3 (2024): Desember 2024
Publisher : Teras Kampus as a member of PT Palem Edukasi Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69616/maindo.v1i3.32

Abstract

This community service activity is a training activity on the manufacture of health and food products that have the opportunity to improve the community's economy as well as providing education on the use of moringa leaves as face masks and papaya fruit as an analog tomato sauce to mothers in Tondonggeu Village, Nambo District, Kendari City. Tomato sauce is generally made from tomato raw materials, but it can also be made from other ingredients so it is called an analog tomato sauce. Tomato sauce is generally made from tomato raw materials, but it can also be made from other ingredients so it is called an analog tomato sauce. Papaya as a substitute for tomatoes in making analog tomato sauce can be done because papaya has characteristics like tomatoes, namely color, organic acid content, and viscosity, even papaya has a higher vitamin C level than tomatoes.  Moringa leaves contain many vitamins, including vitamin A, vitamin C, amino acids and antioxidants such as flavonoids, quercetin and zeatin which function to maintain dry skin hydration and prevent the appearance of wrinkles, because it helps fight free radicals, so it is very good to be used as a cosmetic ingredient in the form of a face mask. Participants in this training involved residents of Tondonggeu village, Thematic KKN students and a team of accompanying lecturers from the Biotechnology and Biology study program of FMIPA Halu Oleo University. The training methods used are lectures, demonstrations and discussions. The lecture method was carried out with the aim of providing information and knowledge about moringa masks and analog ketchup. The demonstration aims to provide skills to mothers through the practice of directly processing moringa leaves into masks and papaya fruits into analog tomato sauce while the discussion is intended to show the activeness and understanding of the community during the training process. The results of the training on making masks and analog tomato sauce carried out by mothers are in the good category with an average success of 85%, their response to the implementation of the training is very good as seen from the enthusiasm of the residents to participate in the activity from the beginning to the end. This training activity can increase knowledge and improve mothers' skills in producing moringa masks and papaya analogue tomato sauce.
Training of Microbe Cell Visualization from Fermentation Food at UPT. Integrated Laboratory UHO Muhamad Azwar Syah; Sapto Raharjo; La Ode Muhammad Iman Sulaiman; Jamili; Nasaruddin
Majalah Pengabdian Indonesia Vol. 1 No. 3 (2024): Desember 2024
Publisher : Teras Kampus as a member of PT Palem Edukasi Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69616/maindo.v1i3.236

Abstract

Indonesia has diverse local food commodities that are valuable as a national food source. Microbes serve an important part in food processing, but they can also be harmful and damage food products. The aim of this training is to provide students with skills in understanding microbial cell visualization techniques in food fermentation. Tempeh and the probiotic drink Yakult as sources of fungi and bacteria, respectively, were used in this training. There are four steps for this training consisting of preparation, visualization of mold on tempeh, visualization of bacteria on Yakult as well as training assessment. The findings indicate that students can identify the complex morphology of tempeh mold, which consists of aseptate hyphae, spores, and sporangiophores that sustain the sporangium. Moreover, they exhibited a bacil shape as the morphology of bacteria from Yakult under a light microscope with 400x magnification. Bacterial staining is recommended to visualize bacteria more clearly than without staining. This occurs because bacteria are smaller than mold and have a more translucent hue. The findings of the activity assessment revealed that participants were able to follow the instruction well. This is demonstrated by success indicators in displaying mold morphology, which includes hyphae, sporangiophores, sporangia, and spores. Participants were also able to identify the structure of rod-shaped bacterial organisms, known as bacilli.
Analysis of the Molecular Structure of Lipase-Dependent Chaperone from Ralstonia pickettii Strain BK6 Azwar Syah, Muhamad; Ambardini, Sri; Jamili, Jamili; Muzuni, Muzuni; Trisandy, Darul
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33770

Abstract

Several biotechnology industries are exploring the characteristics of lipase-dependent chaperones due to their distinctive biochemical traits. This study aimed to employ bioinformatics to analyze the molecular structure of Ralstonia picketii BK6's lipase-dependent chaperon (LipRM). The sequence mapping and amino acid distribution were examined using BioEdit (version 7.0.9.1). SignalP 5.0 and Interpro are employed for signal peptide detection, whereas Swiss-Model and VMD 1.9.2 are used for molecular dynamics modelling. The results showed that the Shine-Dalgarno sequence was discovered in the LipRM promoter, seven nucleotides upstream of the initiation codon (AUG) with the 5'-AGGAGA-3', and has a terminator region that facilitates the formation of a secondary structure. The protein's 3D structure prediction results indicate differences in the alpha helix chains (residues 166-174 and 254-271) between LipRM and the reference lipase. LipRM's molecular structure comprises a detachable signal peptide, and with variations in helix alpha chain conformation and ligand geometry.
Assistance in Mastering PCR Methods for Olympiad Students at MAN Insan Cendekia in Kendari City Syah, Muhamad Azwar; Raharjo, Sapto Raharjo; Jamili; Ambardini, Sri; Jeni; Syahrir; Yamin Yaddi; Munadi, La Ode Muh
Majalah Pengabdian Indonesia Vol. 2 No. 1 (2025): April 2025
Publisher : Teras Kampus as a member of PT Palem Edukasi Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69616/maindo.v2i1.254

Abstract

Madrasah Aliyah Negeri Insan Cendekia (MAN IC) in Kendari City is one of the OSN (National Science Olympiad) participants in the subject of biology, which is required to understand the idea and practice of PCR (Polymerase Chain Reaction). However, there are insufficient skilled PCR teachers at the school, and no PCR facilities are currently accessible. The goal of this mentoring is to provide an in-depth understanding of the concept of PCR as well as full training on how to use PCR effectively. The method consists of two components: community education and training. The introduction of the PCR concept in this community service includes genetic material, DNA concepts, genes, genomes, DNA isolation, and DNA replication, while the training material includes solution taking techniques, PCR solution preparation, PCR usage procedures, PCR result visualization with electrophoresis, and data interpretation. The PCR has three main steps consisting of denaturation, annealing and extension which are carried out for 30-35 cycles with different temperatures at each step. The evaluation results showed that this mentoring helped the student understand PCR and was beneficial for the OSN competition due to its relevance to the competition syllabus. Moreover, the student also received a bronze medal in the biology competition.
Mangrove Nursery Training fo Marine and Fisheries Student Group in Buton Tengah Regency Adi Parman Rudia, La Ode; Izal; Muhamad Azwar Syah; Phradiansyah; La Ode Abdul Fajar Hasidu
Majalah Pengabdian Indonesia Vol. 1 No. 1 (2024): April 2024
Publisher : Teras Kampus as a member of PT Palem Edukasi Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69616/m.v1i1.6

Abstract

Mangrove degradation that occurs in various regions of Southeast Sulawesi, especially in Mawasangka sub-district, Central Buton Regency, is caused by several factors, one of which is the conversion of mangroves into ponds. Real action is needed in mangrove rehabilitation efforts. Some concrete actions in mangrove rehabilitation efforts include species recognition activities as well as mangrove nurseries, which can later be used as a source of seeds for mangrove planting activities. This activity aims to provide understanding to students of the Marine Science and Fisheries Science Study Program regarding the types of mangroves, seed selection, and how to seed mangroves. The methods used in carrying out this activity were a survey of mangrove locations, introduction of mangrove species, selection of seeds, nurseries, and evaluation of activities. A mangrove location survey was carried out to inspect mangrove areas that were open due to conversion into ponds. Species introduction is carried out directly in the mangrove area around the pond. Seed selection is carried out on mangrove propagules that meet the requirements as a seed source. Mangrove seeding is carried out on previously selected mangrove species. Meanwhile, evaluations are carried out 30 days after planting, to see the level of success of mangrove seedlings in growing. The results obtained were that there were 16 types of mangroves, with 4 types of mangroves used as seed sources, namely R. apiculata, R. mucronata, C. tagal, and B. gymnorrhiza. There are a total of 1200 mangrove seedlings that have successfully grown, with a growth success rate of 100%. Apart from that, the participants' capacity in the aspect of introducing types of mangroves, how to seed several types of mangroves, and how to select good seeds has increased. Meanwhile, the participants had a very good understanding of the function and role of mangroves for the environment and society.
PENINGKATAN PENGETAHUAN PETERNAK MELALUI PENYULUHAN SAPI BALI PADA SISTEM PEMELIHARAAN SEMI-INTENSIF DI KECAMATAN KONDA, KABUPATEN KONAWE SELATAN Tasse, Andi Murlina; Rusdin, Muh.; Surahmanto, Surahmanto; Sahaba, La Ode; Kete, Surya Cipta Ramadhan; Jabuddin, La Ode; Mardin, Mardin; Syah, Muhamad Azwar; Sensu, La; Sakti, Abdul; Indi, Amiluddin
BESIRU : Jurnal Pengabdian Masyarakat Vol. 1 No. 11 (2024): BESIRU : Jurnal Pengabdian Masyarakat, November 2024
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/fgdhyr37

Abstract

Program penyuluhan tentang manajemen seleksi bibit dan sistem pemeliharaan semi-intensif sapi Bali dilaksanakan di Kecamatan Konda, Kabupaten Konawe Selatan, bertujuan untuk meningkatkan pengetahuan dan keterampilan peternak dalam meningkatkan produktivitas ternak. Metode yang digunakan meliputi ceramah, demonstrasi lapangan, diskusi, dan pendampingan intensif. Hasil kegiatan menunjukkan peningkatan signifikan pada pemahaman peternak mengenai kriteria seleksi bibit unggul, seperti kesehatan fisik, struktur tubuh, dan potensi genetik. Peternak juga lebih terampil menerapkan sistem pemeliharaan semi-intensif, yang melibatkan pemberian pakan tambahan dan manajemen kandang yang lebih baik. Dampaknya, terjadi peningkatan bobot badan sapi sebesar 10-15% dalam tiga bulan pasca pelatihan serta peningkatan pendapatan peternak sekitar 20-30%. Namun, masih terdapat tantangan dalam akses pakan berkualitas dan adaptasi terhadap teknologi baru. Program ini membuktikan pentingnya penyuluhan berkelanjutan untuk meningkatkan kesejahteraan peternak di wilayah tersebut.
Molecular Identification and Characterization of Lipase-Producing Bacteria Isolated from Wawolesea Hot Spring, Southeast Sulawesi Sapto Raharjo; Muhammad Azwar Syah; Andi Tantri; Tien Tien; Lina Lestari
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.215-220

Abstract

The Wawolesea hot spring in Southeast Sulawesi represents an extreme ecosystem characterized by high temperature and salinity, providing a potential habitat for thermo-halophilic bacteria that produce industrial enzymes, particularly lipase. Here, we carried out molecular identification of lipase-producing bacteria isolated from the Wawolesea hot spring using 16S rRNA gene analysis. The sequence comparison showed that isolate K.111 was the most closely related to Bacillus thuringiensis. This supports the hypothesis that the Wawolesea hot spring harbors thermo-halophilic bacterial communities with potential applications in industrial biocatalysis.
Kemampuan Induksi Resistensi Campuran Bakteri Endofit Asal Tumbuhan Liar Terhadap Penyakit Layu Fusarium Pada Tanaman Cabai Nur Isnaini Ulfa; Andi Khaeruni; Vit Neru Satrah; Gusnawaty HS; Nuriadi Nuriadi; Muhammad Botek; Muhamad Azwar Syah; Eka Febrianti; Rian Arini; La Ode Hikmal Abid; Tri Endrawati
Jurnal Sains Agro Vol 11, No 1 (2026): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/jsa.v11i1.2037

Abstract

Penyakit layu Fusarium yang disebabkan oleh F. oxysporum merupakan salah satu kendala utama produksi cabai merah (Capsicum annuum L.) di Sulawesi Tenggara, dengan potensi kerugian panen hingga 50%. Pengendalian kimiawi menggunakan fungisida sintetis menimbulkan dampak negatif berupa pencemaran lingkungan dan resistensi patogen, sehingga diperlukan alternatif pengendalian hayati yang ramah lingkungan. Penelitian ini bertujuan untuk mengetahui kemampuan campuran bakteri endofit asal tumbuhan liar dalam menginduksi ketahanan tanaman cabai terhadap penyakit layu Fusarium. Penelitian ini dilaksanakan di Laboratorium Proteksi Tanaman Unit Fitopatologi dan di Rumah Kaca Lahan I Fakultas Pertanian Universitas Halu Oleo pada Bulan Juli sampai September 2025. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 8 perlakuan  yang diulang 3 kali sehingga terdapat total 24 unit percobaan, setiap unit terdiri dari 5 tanaman, sehingga populasi dalam penelitan ini adalah 120 tanaman. Penelitian menggunakan tiga isolat bakteri endofit asal tumbuhan liar, yaitu BK-3(2) dari batang kersen, BSR-7(1) dari batang suruhan, dan ASR-7(1) dari akar suruhan, yang diaplikasikan secara tunggal maupun kombinasi. Parameter yang diamati meliputi aktivitas enzim peroksidase, kadar asam salisilat, aktivitas enzim polifenol oksidase, dan keparahan penyakit layu Fusarium. Data dianalisis dengan sidik ragam dan apabila terdapat pengaruh nyata, maka dilanjutkan dengan Uji Jarak Berganda Duncan (UJBD) pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa seluruh perlakuan bakteri endofit terbukti mampu menginduksi ketahanan tanaman cabai. Perlakuan kombinasi BK-3(2)+BSR-7(1) dan BK+ASR+BSR secara konsisten memberikan hasil terbaik dalam meningkatkan ketiga parameter enzim pertahanan tersebut. Tingkat keparahan penyakit pada perlakuan kombinasi BK-3(2)+BSR-7(1) dan BK+ASR+BSR tercatat paling rendah, yakni masing-masing 13,33%, dibandingkan kontrol sebesar 36,00%. Bakteri endofit menginduksi ketahanan tanaman melalui mekanisme Induced Systemic Resistance (ISR) dan Systemic Acquired Resistance (SAR). Disimpulkan bahwa konsorsium bakteri endofit asal tumbuhan liar efektif menginduksi resistensi tanaman cabai terhadap penyakit layu Fusarium.
Co-Authors Adi Parman Rudia, La Ode Ahmad, Sitti Wirdhana Amiludin Indi Andi Khaeruni Andi Murlina Tasse Andi Tantri Andreas Adhi Satya Antonius Suwanto Eka Febrianti Esti Puspitasari Gusnawaty HS Hasidu, La Ode Abdul Fajar Izal Jabuddin, La Ode Jamili Jamili Jamili Jeni Karya, Adi La Ode Hikmal Abid La Ode Muhamad Munadi La Ode Muhammad Iman Sulaiman Lina Lestari Mardin, Mardin Muh. Rusdin Muhammad Botek Muhsin Muhsin Muzuni, Muzuni Nasaruddin NISA RACHMANIA MUBARIK Nur Arfa Yanti Nur Isnaini Ulfa Nuriadi Nuriadi Phradiansyah Raharjo, Sapto Raharjo Rian Arini Sakti, Abdul Sapto Raharjo Sapto Raharjo Sensu, La Sri Ambardini Surahmanto Surya Cipta Ramadhan Kete Syahrir Tien Tien Tri Endrawati Trisandy, Darul Vit Neru Satrah Yamin Yaddi