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All Journal PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Biocaster : Jurnal Kajian Biologi
Sasmita, Rani
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Immunology & microbiology Law, Crime, Criminology & Criminal Justice Public Health Social Sciences

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Introduksi Teknologi DNA Barcoding kepada Staf UPTD Perikanan Budidaya Air Payau dan Laut Karang Intan: Introduction of DNA Barcoding Technology to UPTD Staff of Brackish and Marine Aquaculture Karang Intan Sasmita, Rani; Hidayaturrahmah, Hidayaturrahmah; Badruzsaufari, Badruzsaufari; Firnanda, Muhammad Riyan; Fitri, Muhammad
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 4 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i4.7056

Abstract

Partners in community service activity are UPTD Brackish and Marine Aquaculture, Karang Intan, one of the centers under South Kalimantan Province that specializes in the hatchery of local fish such as snakehead (Channa striata), common carp (Cyprinus carpio), and climbing perch (Anabas testudineus). The problem in the partner is fish farming that still relies on seeds from nature, which causes several problems, such as slow seed growth, decreased production quality, disease attacks and hereditary abnormalities that are getting higher due to interbreeding. Based on these problems, the team collaborated with the UPTD for Brackish and Marine Aquaculture, Karang Intan, to improve the genetic quality of fish by introducing DNA barcoding techniques, especially at the spawning stage. The introduction of DNA barcoding technology is carried out through (1) classical counseling and group discussions, 2) training and assistance covering technical aspects of DNA barcoding (fish DNA isolation, DNA amplification, visualization of PCR products with electrophoresis, to the sequencing). Based on the evaluation, 100% of the participants were enthusiastic and realized the importance of DNA barcoding techniques to produce superior broodstock and fish fry, especially for snakehead, common carp, and climbing perch commodities.
Insights into Evolutionary Relationships of Proboscis Monkeys Using In Silico Mitochondrial DNA Analysis Sasmita, Rani; Badruzsaufari, Badruzsaufari; Pangestika, Intan Widya
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.688

Abstract

This study investigates the evolutionary position of the proboscis monkey (Nasalis larvatus) within the Colobinae subfamily using in silico phylogenetic analysis of three mitochondrial protein-coding genes (COI, COII, and COIII). Seventy sequences retrieved from GenBank were aligned and analyzed using the Maximum Likelihood method with 1,000 bootstrap replicates. All genes exhibited conserved lengths and strong AT bias (59,21-60,68%), reflecting typical mitochondrial genome characteristics shaped by asymmetric replication and purifying selection. Genetic distance analyses revealed high intraspecific consistency in Nasalis larvatus, while demonstrating marker dependent variation in phylogenetic resolution. COII and COIII provided clearer intergeneric separation compared to the more conserved COI marker. Phylogenetic reconstruction showed topological variation among markers, however, a consistent evolutionary signal emerged, with Nasalis larvatus clustering robustly with Simias concolor across all gene trees. This pattern supports the monophyly of the odd nosed monkey clade and aligns with genome scale phylogenomic evidence. Overall, this study highlights the limitations of single gene analyses and emphasizes the importance of multi marker or genome scale approaches for resolving complex evolutionary relationships. These findings provide foundational insights into the evolutionary history of Nasalis larvatus and reinforce the relevance of mitochondrial markers in primate conservation genetics.
Co-Authors Badruzsaufari Badruzsaufari Firnanda, Muhammad Riyan Hidayaturrahmah Hidayaturrahmah Muhammad Fitri, Muhammad Pangestika, Intan Widya