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All Journal Biospecies Jurnal Perlindungan Tanaman Indonesia JURNAL PENGABDIAN KEPADA MASYARAKAT
Harahap, Nafsiyah Agustina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Social Sciences Other

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Bioassay Method Development to Test Sitophilus oryzae Sensitivity against Phosphine Harahap, Nafsiyah Agustina; Witjaksono, Witjaksono; Martono, Edhi; Trisyono, Y. Andi
Jurnal Perlindungan Tanaman Indonesia Vol 27, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.75904

Abstract

Sitophilus oryzae is a cereal product pest found in warehouses. The presence of this pest negatively impacts the quality and quantity of stored rice. The common method used to control warehouse pests is fumigation. However, frequent use of insecticides will trigger resistance within target pest populations. This study aimed to develop a bioassay method based on the Food Agriculture Organization (FAO) protocols and modify bioassay protocols to determine S. oryzae susceptibility of populations collected from traditional markets across Yogyakarta. Field populations were collected from eight traditional markets in Yogyakarta (Lempuyangan Market [Kapanewon Danurejan], Kranggan Market [Kapanewon Jetis], Caturtunggal Market [Kapanewon Depok], Godean Market [Kapanewon Godean], Imogiri Market [Kapanewon Imogiri], Pleret Market [Kapanewon Pleret], Kasihan Market [Kapanewon Lendah], and Brosot Market [Kapanewon Galur]). The reference population was obtained from the Southeast Asian Regional Centre for Tropical Biology(SEAMEO BIOTROP). The bioassay was carried out following FAO protocols by using a glass jar fumigation chamber (volume 2 L) which was a modification of the desiccator of FAO recommended method number 16. This test used the lowest testable dose for this method: 0.01 × 10-2 mg/2L or 0.5 × 10-2 µg/L tested on 100 imagoes which were divided into five replications and obtained 100% mortality on the second day (48 hours). Results showed that the FAO fumigation chamber method could not be used in the test, so modifications were carried out to determine tested doses by changing the volume of the fumigation container. The test used three container volumes, including 20, 60, and 80 L. Result from 20 L container showed 82-100% mortality, 60 L containers showed 69-100% mortality, and 80 L containers showed 24-79% mortality. Results from 80 L container was suitable for the bioassay because it was able to test the lowest dose on reference insects. Eight doses ranging from 0.125 to 1.625 × 10-2 µg/L, and an untreated control were used to determine the LD50 of phosphine on each population. Insects were fumigated for 48 hours and then removed to observe mortality. The LD50 of the reference population was 0.27 × 10-2 µg/L while 0.29-0.54 × 10-2 µg/L for field populations. These findings indicate that S. oryzae populations collected from traditional markets in Yogyakarta were still susceptible to phosphine.
PENGARUH PERBEDAAN KEPADATAN POPULASIRhyzopertha dominicaTERHADAP JENIS DAN KERUSAKAN SEREALIA Harahap, Nafsiyah Agustina
Biospecies Vol. 18 No. 2 (2025): Juli 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i2.46214

Abstract

The study aimed to determine the effect ofRhyzopertha dominicapopulation density on thetype and extent of damage to cereals. The study was conducted at the Plant Pests andDiseases Laboratory, Agroecotechnology Study Program, Faculty of Agriculture,Malikussaleh University. The study was conducted in the form of alaboratory experimentwith two types of treatments arranged in a Completely Randomized Design. The cereal typetreatment consisted of two levels, namely sorghum and wheat. The types of cereal varietiesused in the study were the tropical wheat variety Dewata and the sorghum variety Super-2obtained from the Cereal Crops Research Institute, Maros, South Sulawesi. The populationdensity treatment ofR. dominicaconsisted of four levels, namely 5, 10, 15, and 20 pairs ofadults per 150 g of cereal. Each treatment combination was repeated three times, so thatthere were 24 experimental units.The results showed that increasing the population densityofR. dominicasignificantly affected the number of F1, the percentage of cereal damage, theamount of powder, andweight loss during storage. The density of 20 pairs of adultsproduced the highest number of F1 and damage. Cereal type also influenced theseparameters, with wheat showing higher levels of damage than sorghum, indicating thatwheat is preferred byR. dominica.However, there was no significant interaction betweenpopulation density and cereal type on any of the observed parameters. Data were analyzedusing analysis of variance and the DMRT test at the 0.05 level.
Peningkatan Pengetahuan Petani tentang Penggunaan Pestisida Nabati di Desa Rawang Lama untuk Mengendalikan Hama Rhiki Budianto; Harahap, Nafsiyah Agustina; Ardina; Hilda Yanti Br Torus Pane; Muhammad Safrizal; Oktopanda; Ramadani; Durahman Marpaung
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 15 No 1 (2025): Juli 2025
Publisher : LPPM UNINUS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30999/jpkm.v15i1.3681

Abstract

Penggunaan pestisida kimia yang berlebihan dalam praktik pertanian telah menimbulkan dampak negatif terhadap kesehatan manusia dan lingkungan. Pestisida nabati menjadi alternatif ramah lingkungan dalam pengendalian hama tanaman. Penelitian ini bertujuan untuk mengetahui efektivitas penyuluhan dalam meningkatkan pengetahuan petani tentang penggunaan pestisida nabati di Desa Rawang Lama, Kabupaten Asahan. Metode penelitian yang digunakan adalah pre-eksperimen dengan desain one-group pretest-posttest. Sebanyak 30 petani menjadi responden, yang dipilih secara purposive. Instrumen yang digunakan berupa kuesioner pilihan ganda yang diberikan sebelum dan sesudah penyuluhan. Hasil menunjukkan peningkatan rata-rata nilai pretest dari 53,0 menjadi 81,3 pada posttest. Hasil uji Paired Sample t-Test menunjukkan nilai signifikan (p-value) sebesar 0,000 (< 0,05), yang mengindikasikan adanya peningkatan pengetahuan yang signifikan setelah intervensi. Penyuluhan terbukti efektif dalam meningkatkan pemahaman petani mengenai pengertian, jenis, dan manfaat pestisida nabati seperti serai dan daun sirsak dalam pengendalian hama. Temuan ini menegaskan bahwa edukasi berperan penting dalam mendorong petani mengadopsi praktik pertanian yang lebih berkelanjutan dan ramah lingkungan. Kata kunci: Pestisida nabati, penyuluhan, pengetahuan petani, pengendalian hama, pertanian ramah lingkungan, Desa Rawang Lama
Co-Authors Ardina Durahman Marpaung Edhi Martono Hilda Yanti Br Torus Pane muhammad safrizal Oktopanda Ramadani Rhiki Budianto Witjaksono Witjaksono Y. Andi Trisyono