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p-Index From 2021 - 2026
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This Author published in this journals
All Journal IDEAS: Journal on English Language Teaching and Learning, Linguistics and Literature Journal of Health (JoH)
Khairun Nissa
Unknown Affiliation
Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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Optimasi Kodon dan Konstruksi Plasmid Rekombinan Protein RBD SARS-CoV-2 pada E. coli BL21(DE3): Codon Optimization and Construction of Recombinant Plasmid for SARS-CoV-2 RBD Protein on E. coli BL21(DE3) Purnamasari, Elly Widyarni Eka; Dini Wahyu Kartika Sari; Tenri Ashari Wanahari; Khairun Nissa; Kusumawati, Asmarani
Journal of Health (JoH) Vol 11 No 1 (2024): Journal of Health (JoH) - January
Publisher : LPPM STIKES Guna Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/joh.v11n1.715

Abstract

Coronavirus Disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) resulted over 7 million deaths worldwide. Comprehensive strategies are necessary to combat this pandemic, especially vaccination to mitigate further infection and devastation. The development of vaccines hinges upon the utilization of immunogenic proteins from the SARS-CoV-2 virus, with particular emphasis on the receptor-binding domain (RBD) located within the spike glycoprotein’s subunit 1. This protein plays a key role in the virus’s interaction with host cells via ACE2 receptor to infect the host along with the subunit 2 to mediate the cell membrane fusion. While there are multiple vaccines in existence, there is a requirement to create more affordable and accessible vaccine alternatives, especially in developing countries. Potential improvements can be implemented within the vaccine development paradigm, including the exploration of recombinant protein-based vaccines as a viable alternative. In the present study, we optimized the RBD gene sequence as the critical step to construct a recombinant plasmid for heterologous prokaryotic protein expression in Escherichia coli BL21(DE3). The optimized codon then inserted into the plasmid vector pET-15b and constructed a recombinant plasmid for transformation into cell host by heat-shock method. The study was evaluated and validated through colony PCR, plasmid isolation, and sequencing. These findings offering a foundational basis for further research in the expression and purification of RBD SARS-CoV-2 protein in E. coli BL21(DE3) as a candidate for subunit recombinant protein vaccine or diagnostic tool in COVID-19 research.
Analysis of Language Policy and Language Ideology in Medan City Public Spaces: A Study of Linguistic Landscape Mandra, Mandra Saragih; Harisma, Rita; Khairun Nissa; Toni Suhendra Saragih, M Afiv
IDEAS: Journal on English Language Teaching and Learning, Linguistics and Literature Vol. 13 No. 1 (2025): IDEAS: Journal on English Language Teaching and Learning, Linguistics and Lite
Publisher : Institut Agama Islam Negeri Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24256/ideas.v13i1.6966

Abstract

This study investigates the realization of language policy and language ideology reflected in the linguistic landscape of Medan City's public space. Using a mixed methods approach and an explanatory sequential design, this study integrates quantitative data from the documentation of 300 written texts in five sub-districts and qualitative data from interviews with stakeholders and analysis of policy documents. The results show the dominance of Indonesian (42.7%), followed by multilingual texts (34.7%) and texts in foreign languages such as English and Mandarin (18.3%). There is a discrepancy between the national language policy and linguistic practices in the public sphere, especially in the commercial sector where many foreign languages are used for pragmatic economic reasons. Three dominant language ideologies were found, namely language nationalism, economic pragmatism, and ethnic identity, which compete with each other in influencing language practices. This research demonstrates the dialectical relationship between language policy, language ideology and linguistic practice, and emphasizes the importance of an inclusive and adaptive policy approach to the linguistic diversity of multiethnic societies. The findings contribute to the development of a more contextualized and effective theory of linguistic landscapes and language policy practices.
Analysis of Language Policy and Language Ideology in Medan City Public Spaces: A Study of Linguistic Landscape Mandra, Mandra Saragih; Harisma, Rita; Khairun Nissa; Toni Suhendra Saragih, M Afiv
IDEAS: Journal on English Language Teaching and Learning, Linguistics and Literature Vol. 13 No. 1 (2025): IDEAS: Journal on English Language Teaching and Learning, Linguistics and Lite
Publisher : Institut Agama Islam Negeri Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24256/ideas.v13i1.6966

Abstract

This study investigates the realization of language policy and language ideology reflected in the linguistic landscape of Medan City's public space. Using a mixed methods approach and an explanatory sequential design, this study integrates quantitative data from the documentation of 300 written texts in five sub-districts and qualitative data from interviews with stakeholders and analysis of policy documents. The results show the dominance of Indonesian (42.7%), followed by multilingual texts (34.7%) and texts in foreign languages such as English and Mandarin (18.3%). There is a discrepancy between the national language policy and linguistic practices in the public sphere, especially in the commercial sector where many foreign languages are used for pragmatic economic reasons. Three dominant language ideologies were found, namely language nationalism, economic pragmatism, and ethnic identity, which compete with each other in influencing language practices. This research demonstrates the dialectical relationship between language policy, language ideology and linguistic practice, and emphasizes the importance of an inclusive and adaptive policy approach to the linguistic diversity of multiethnic societies. The findings contribute to the development of a more contextualized and effective theory of linguistic landscapes and language policy practices.
Co-Authors Asmarani Kusumawati Dini Wahyu Kartika Sari Harisma, Rita Mandra, Mandra Saragih Purnamasari, Elly Widyarni Eka Tenri Ashari Wanahari Toni Suhendra Saragih, M Afiv