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All Journal JIM ( Journal International Multidiciplinary) Bioma : Jurnal Ilmiah Biologi
Nursyafi, Fauzan Syarif
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Other

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Innovative Utilization of Orange Peel Waste through Modified Citrus Pectin (MCP) as a Preventive Agent Against Atherosclerosis Progression via Galectin-3 (Gal-3) Inhibition: A Comprehensive Literature Review on In Vitro and In Vivo Study Designs Nursyafi, Fauzan Syarif; Febrian, Lamirza Rasyid Mikzy; Putra, Rivaldi Dwi; Renato, Hagil Alghufron; Kurniawan, Dio
JIM - Journal International Multidisciplinary Vol. 2 No. 2 (2024)
Publisher : Rumah Jurnal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58794/jim.v2i2.889

Abstract

Atherosclerosis is an arterial disease often associated with lipids and other metabolic alterations. Various factors are involved in developing atherosclerosis, including Galectin-3 (Gal-3). Pre-clinical studies have shown that Modified Citrus Pectin (MCP), a modified polysaccharide from citrus peels, has the potential of inhibiting Gal-3 and countering the development of atherosclerosis. Thus, this review aims to analyze the role of MCP on inhibiting and regulating Galectin-3 (Gal-3) as an alternative therapeutic target. We examined valid pre-clinical experimental study designs taken from the databases Science Direct, Taylor & Francis, PubMed, and Google Scholar, with publication dates ranging from 2014 to 2024. 15 research articles meet the established inclusion criteria. Our review found that Gal-3 levels rose during atherosclerosis development, triggering proteins that disturbs cardiovascular physiology. Additionally, Gal-3 targeted inhibition using MCP has shown to have therapeutic effects that alleviates hypertrophy and plaque’s size. In essence, MCP emerges as a promising alternative therapy targeting Gal-3 in atherosclerosis progression.
ISOLATION OF AnsB GENE FRAGMENT ENCODING L-ASPARAGINASE 2 ENZYME FROM Serratia plymuthica UBCF_13 AND IT’S IN-SILICO DOMAIN CHARACTERISTIC Ananda, Abi Awfa Rahman; Nursyafi, Fauzan Syarif; Aliya, Lisana Shidiqqin; Hasibuan, Imron Martua; Alioes, Yustini; Endrinaldi , Endrinaldi; Adrial, Adrial; Elmatris , Elmatris; Jamsari, Jamsari; Lily Syukriani
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/n5mfhy69

Abstract

Acute lymphoblastic leukemia(ALL) is one of the cancer diseases often occurs in children and causes high mortality in children. One of the chemotherapy treatment suggested is using L-Asparaginase 2. However due to its difficult production process making this approach expensive for the public. Therefore production technology of this enzyme is crucial enabling cheaper for ALL treatment. This study aimed to isolate the AnsB gene sequence from Serratia plymuthica UBCF_13 and perform its further in-silico analysis. The research was started by designing specific primers for the AnsB gene, isolating the AnsB gene fragment using PCR-based approach, sequencing the AnsB gene fragment, cloning the fragment to the plasmid vector and further transformed into E. coli DH5α cell. Further data analysis was carried out using some bioinformatics tools such as BLAST, MEGA X, I-TASSER InterPro. Sequence data result successfully verified that the full length of AnsB gene is 1047 bp. InterPro analysis indicated that the L-Asparaginase 2 from S. plymuthica UBCF_13 has 2 domains, namely L-Asparaginase N-terminal spanning from amino acid 26 to 216, while its C-terminal spanned from amino acid 235 to 345. The physical fragment of the gene was also successfully cloned to the pGEM-T Easy vector and subsequently transformed into E. coli DH5α cell. This result provided information for alternative sources of L-Asparaginase 2 and it’s possible engineering.
Co-Authors Adrial Adrial, Adrial Aliya, Lisana Shidiqqin Ananda, Abi Awfa Rahman Elmatris , Elmatris Endrinaldi , Endrinaldi Febrian, Lamirza Rasyid Mikzy Hasibuan, Imron Martua Jamsari Jamsari Kurniawan, Dio Lily Syukriani Putra, Rivaldi Dwi Renato, Hagil Alghufron Yustini Alioes