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All Journal Dental Journal (Majalah Kedokteran Gigi) Journal of Fisheries & Marine
Ibrahim, Zurairah
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Biochemistry, Genetics & Molecular Biology Dentistry

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Journal : Dental Journal (Majalah Kedokteran Gigi)

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Cervical vertebral maturation stage and Demirjian index for assessment of skeletal and dental maturation for children’s growth stages Pradopo, Seno; Nuraini, Prawati; Rahmawati, Luluk; Ibrahim, Zurairah
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 1 (2025): March
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i1.p60-65

Abstract

Background: Determining age is essential in various fields, particularly pediatric dentistry. A reliable method for assessing an individual's growth and development involves evaluating their chronological and biological age. Biological age can be determined by examining skeletal or dental maturation. Accurately assessing growth potential and timing of growth spurts is crucial for several clinical situations, especially in the planning and outcomes of treatments like orthodontic therapy. Purpose: This study uses common radiographic ortho-diagnosis techniques to analyze the accuracy of cervical vertebral maturation stages (CVMS) and the Demirjian index methods in evaluating children's growth and development stages. Methods: The CVMS assessment on cephalometric radiography was conducted using the Bacetti method, which includes six stages. In addition, the mandibular second molars' calcification stages were evaluated using the Demirjian index method, which encompasses stages A to H on panoramic radiography. Following this, skeletal and dental maturation accuracy was analyzed using the Statistical Package for Social Sciences application. Results: This study revealed differences in the chronological age corresponding to each stage of calcification of the mandibular second molars and the CVMS. Additionally, CVMS was found to be the most accurate method for assessing age in children. Furthermore, the right side was generally preferred over the left at the calcification stages of the mandibular second molars. Conclusion: Using CVMS to assess skeletal maturation provides a more accurate determination of growth and developmental stages in children than the Demirjian index.
Amelogenin and alkaline phosphatase expression in ameloblast after saltwater fish consumption in pregnant mice (Mus musculus) Christiono, Sandy; Pradopo, Seno; Sudiana, I Ketut; Hutami, Islamy Rahma; Mayangsari, Regilia Shinta; Rochmah, Yayun Siti; Ibrahim, Zurairah
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p166-171

Abstract

Background: The intricate process of tooth formation during embryonic development ensures sufficient nutrition for the growth of healthy dental tissues. Amelogenin and alkaline phosphatase (ALP) are serine proteinases secreted by the ameloblast during the transition and maturation phases of the amelogenesis process. Consumption of saltwater fish is predicted to increase the expression of amelogenin and ALP in ameloblast cells during tooth formation. Only now have the function of each gene, tooth-forming cells, and the proteins they produce in the biomolecular amelogenesis of tooth enamel, which began during prenatal development, been clarified. Purpose: This study aims to determine how saltwater fish powder affects the ability of mother mice to increase the expression of amelogenin and ALP in cell ameloblast. Methods: Using a completely randomized design, this study was experimental and aimed to examine the effects of sardine (Sardinella fimbriata), splendid ponyfish (Leiognathus splendens), and tuna (Euthynnus affinis) powder. As samples, twenty-four female mice (Mus musculus) were used. Two groups of mice were created: group 1 (2.14 mg/0.5 ml) and the control group. The expression of amelogenin and ALP was determined using immunohistochemistry (IHC) and t-test (p0.05). Results: Expression of ameloblast was significantly different between the treatment and control groups (p0.05). Conclusion: The consumption of saltwater fish reduces the amelogenin and ALP expressions of mouse fetal ameloblast cells during tooth development in vivo.
Co-Authors Arlina Nurhapsari Indasari, Novia Islamy Rahma Hutami, Islamy Rahma Mayangsari, Regilia Shinta Prawati Nuraini Rahmawati, Luluk Rochmah, Yayun Siti Rochmah S.Pd. M Kes I Ketut Sudiana . Sandy Christiono Seno Pradopo Suparmi Yayun Siti Rochmah Yuniar, Savira Nurazky