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All Journal Medical Laboratory Technology Journal Anakes: Jurnal Ilmiah Analis Kesehatan Jurnal Kefarmasian Indonesia
Hariutami, Desi
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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The Relationship of Plasmodium sp Density to Platelet Count and Hemoglobin Levels in Malaria Patients at Bhayangkara Hospital TK I Pusdokkes Police Kramat Jati, Indonesia Inderiati, Dewi; Oktapıa, Nısa; Nida, Wadhatun; Zuraida, Zuraida; Hariutami, Desi
Medical Laboratory Technology Journal Vol. 10 No. 2 (2024): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i2.613

Abstract

Malaria is an acute and chronic disease. Globally, as many as 247 million positive cases of malaria were reported in 84 malaria-endemic countries. Indonesia is a malaria-endemic country with a total of 443,530 cases with a malaria prevalence of 89% reported from Papua province. This study examined the correlation between Plasmodium sp density platelet count and hemoglobin levels. The type of research used in this study was observational analytics with a cross-sectional study approach design. The sample and population in this study were malaria-positive patients who had their platelet count and hemoglobin levels checked as recorded in the report from Bhayangkara Hospital Tk I Pusdokkes Polri Kramat Jati, Indonesia, namely using medical record data from months January to December a total of 54 samples with data analysis in this study using descriptive univariate analysis and bivariate analysis using the Fisher Exact test. This study showed that the density of Plasmodium sp with the number of platelets had a significant result, namely 0.088, which indicates that the density of Plasmodium sp does not affect the platelet count. In contrast, the density of Plasmodium sp with hemoglobin levels had a significant result, namely 0.023, which indicated a lower Hb level of malaria sufferers. The higher the level of Plasmodium density.
The Effect of Different Solvents on the Content of Black Cumin Seed Extract (Nigella sativa) Hariutami, Desi; Darmawati, Sri; Permana, Atna; Zuraida, Zuraida
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 1, FEBRUARY 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

Seeds of black cumin (Nigella sativa L.), also called "black cumin," are an herbal plant from the Ranunculaceae family. The objective of this study was to determine the yield of black cumin seed extract using 96% ethanol, N-hexane, and ethyl acetate as solvents, as well as to determine the results of phytochemical screening tests and the active ingredient content using GCMS. The stages of the research included the extraction process, phytochemical screening test, and GCMS. The study's findings on the yield of black cumin seed extract using the three solvents showed that the solvent N-Hexane produced the highest yield, at 28.09 percent. Based on phytochemical screening tests, black cumin seeds were positive for containing secondary metabolites such as alkaloids, saponins, phenolic flavonoids, triterpenoids, steroids, and glycoxides. Other potential compounds vizhexadecenoic acid, ethyl ester, Methyl (8e,11e)-8,11- octadecadienoate, 12-octadecadienoic acid (z,z)-, Grape seed oil.
Daya Hambat Ekstrak Bunga Kenanga (Cananga odorata) dan Rosella (Hibiscus sabdariffa L.) terhadap Staphylococcus aureus ATCC BAA - 1026, Pseudomonas aeruginosa ATCC 27853 dan Proteus mirabilis ATCC 2593 Zuraida, Zuraida; Nurdiani, Catu Umirestu; Rahayu, Cahyawati; Permana, Atna; Hariutami, Desi
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 2 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i2.2960

Abstract

Nosocomial infections or healthcare-associated infections (HAIs) are global health problems caused by pathogenic microorganisms, particularly antibiotic-resistant bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis. The increasing incidence of antibiotic resistance highlights the need to explore alternative antibacterial agents derived from natural products. This study aimed to evaluate the antibacterial activity of kenanga (Cananga odorata) and rosella (Hibiscus sabdariffa L.) flower extracts against Staphylococcus aureus ATCC BAA-1026, Pseudomonas aeruginosa ATCC 27853, and Proteus mirabilis ATCC 2593. The antibacterial activity was assessed in vitro using the disc diffusion method with various extract concentrations. The inhibitory activity of kenanga flower extract at a concentration of 100% showed average inhibition zone diameters of 27.50 mm against Staphylococcus aureus, 26.20 mm against Pseudomonas aeruginosa, and 24.20 mm against Proteus mirabilis. Meanwhile, rosella flower extract at a concentration of 100% produced average inhibition zone diameters of 33.10 mm against Staphylococcus aureus, 28.00 mm against Pseudomonas aeruginosa, and 25.20 mm against Proteus mirabilis. The results indicate that both kenanga and rosella flower extracts exhibit antibacterial activity against all tested bacteria, with inhibitory strength varying according to extract concentration. Rosella extract demonstrated higher inhibitory activity against Staphylococcus aureus, whereas kenanga extract showed significant antibacterial activity against specific test bacteria. In conclusion, kenanga and rosella flower extracts have potential as natural antibacterial alternatives to help control bacterial infections associated with nosocomial infections.   Keywords:  Antibacterial, Cananga odorata, Hibiscus sabdariffa L., Nosocomial infection, Antibiotic                                     resistance
Co-Authors Dewi Inderiati Nida, Wadhatun Nurdiani, Catu Umirestu Oktapıa, Nısa Permana, Atna Rahayu, Cahyawati Sri Darmawati Zuraida Zuraida