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ISOLASI DAN IDENTIFIKASI JAMUR MIKORIZA ARBUSKULA (JMA)PADA RHIZOSFER TANAMAN MAKILA (Litsea angulata) DI DESA HATUSUA Malawat, Jasia; Matinahoru, Johan; Hadijah , Miranda
MARSEGU : Jurnal Sains dan Teknologi Vol. 1 No. 5 (2024): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/1.5.2024.496-505

The aim of this research was to identify the types of arbuscular mycorrhizal fungi (JMA) in the rhizosphere of Makila plants (Litsea angulata) and determine the morphological characteristics of each type of fungus (JMA) found. This research uses descriptive methods. Soil samples under the makila plant stands were taken randomly. Observation of spore characteristics was carried out by observing spore samples placed on a glass slide under a microscope. The results of this study showed that the identification of fungal types found 2 genera, namely Glomus and Gigaspora in the rhizosphere of Makila stands, then the results of calculating the number of JMA spores were an average of 980.25 spores per Makila tree and there was no real difference between the number of spores in the rhizosphere of each Makila tree.

EFEKTIVITAS TANAMAN JAGUNG (Zea mays) DAN KACANG HIJAU (Vigna radiata) SEBAGAI MEDIA PEMBIAKAN FUNGI MIKORIZA ARBUSKULA (FMA) Mony, Audy Yanti; Matinahoru, Johan; Hadijah, Miranda
MARSEGU : Jurnal Sains dan Teknologi Vol. 1 No. 6 (2024): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/1.6.2024.596-606

Arbuscular mycorrhizal fungi (AMF) play a role in supporting the growth of various plants under certain conditions. AMF cannot survive in artificial media because they require a host plant for obligate symbiosis, which is necessary during the inoculum production process. The purpose of this study was to determine the effectiveness of corn and green bean roots as a breeding medium for arbuscular mycorrhizal fungi. The method used in this research used a two-factor completely randomized design (CRD). Observation of samples of the growth of corn and green bean seeds was carried out by observing samples placed on glass slides under a microscope. The results of the research showed that corn plants were more effective as a breeding medium for arbuscular mycorrhizal fungi (AMF) compared to green bean plants and the roots of corn plants were infected at a higher rate, namely 57.33% compared to the roots of green bean plants, namely 44.00%.

PENGARUH LARUTAN PUPUK HAYATI ENDOMIKORIZA ASAL AKAR MERANTI TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN KANGKUNG Matinahoru, Johan
MARSEGU : Jurnal Sains dan Teknologi Vol. 1 No. 7 (2024): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/1.7.2024.718-727

Water spinach (Ipomoea sp) is a plant that is widely consumed by people as a vegetable because it is cheap and easy to cultivate. The main problems in growing water spinach are soil fertility and attack by plant pests and diseases. To increase production from kale farming, farmers generally use inorganic fertilizers such as NPK which in reality can cause environmental pollution. For this reason, various efforts to use organic (environmentally friendly) fertilizers such as fungal and bacterial biofertilizers remain the focus of research to date. Mycorrhizal fungi are known to have quite a lot of benefits for plants, especially in providing phosphate and nitrogen nutrients for host plants. In the forestry sector, it is known that the roots of meranti plants (Shorea sp) and the roots of pine plants (Pinus sp) are natural hosts for the growth and development of mycorrhizal fungi. Therefore, the aim of this research is to determine the effect of endomycorrhizal solution fertilizer from meranti roots on the growth and production of water spinach plants. The results of the research show that the endomycorrhizal biofertilizer solution from meranti roots produces growth and production of water spinach plants that are not significantly different from the NPK fertilizer treatment, so from an environmental safety aspect, it is proposed that it is better to use endomycorrhizal biofertilizer because it is environmentally friendly.

PERTUMBUHAN TANAMAN GAHARU (Aquilaria malaccensis lamk) ASAL PROVENANS LAIMU MORNATENG DAN PASSO PADA TANAH INSEPTISOL DI DESA HATUSUA KECAMATAN KAIRATU KABUPATEN SERAM BAGIAN BARAT Samaung, Widya; Matinahoru, Johan; Hadijah, Miranda
MARSEGU : Jurnal Sains dan Teknologi Vol. 1 No. 9 (2024): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/1.9.2024.890-901

The aim of this research is to determine and analyze the ability of agarwood plants to grow on inceptisol soil and to analyze the factors that determine the growth of agarwood plants on inceptisol soil in Hatusua Village, Kairatu District, West seram Regency. The method used in this research is a completely randomized design method, the data collection technique used in this research is in the form of direct observations carried out on a demonstration plot of the source of agarwood (Aquilaria malacccensis lamk) seeds from which 3 trees were taken from each provenance. And data analysis used a completely randomized design and multiple regression analysis. The results obtained from the research were variance analysis of parameters which showed that only the leaf bones were real. The three provenances are determined by three soil factors, namely fertility, humidity and pH. Of the three soil factors, only Laimu provenance has an influence, because it has a larger F-value. And the environmental factor that has the most influence on the Laimu provenance is atmospheric humidity, while the environmental factor that has the most influence on the Mornateng provenance agarwood plant is soil moisture and the most influential environmental factor for the Passo provenance agarwood plant is light intensity.

EKSPLORASI BAKTERI ENDOFIT PADA TANAMAN SAMAMA (Anthocephallus macrophyllus ) Mendez, Syeren; Matinahoru, Johan; Hadijah, Miranda
MARSEGU : Jurnal Sains dan Teknologi Vol. 1 No. 9 (2024): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/1.9.2024.921-937

Bacterial exploration aims to find out how endophytic bacteria associate with the roots of samama plants and find out the morphological characteristics of the endophytic bacteria found. So we can determine the type/family of endophytic bacteria on the Samama plant. Sampling of samama plants (Anthocepalus macropyllus) was taken on the campus of the Faculty of Agriculture, Pattimura University. The parts of the plant samples taken were roots, bark, leaf twigs and leaves. The number of tree samples taken as examples was 3 individual trees. Each plant organ sampled is 200 gr. The data analysis method used is by adjusting the data resulting from the identification of macroscopic and microscopic characteristics with the characteristics of bacterial families that are available according to references. Based on the results of this research, it can be concluded that: there are 9 isolates of endophytic bacteria, namely KB1 KB2 KB3 KB4 A1 A2 A3 A4 and B1 in the root, stem and bark tissues of Samama plants. Endophytic bacterial isolates Kb1, Kb2, kb3, kb4, A2, A4 have similar characteristics to the bacterial group of the genus Bacillus with the characteristics of rod-shaped cells and are classified as gram positive, while isolates Kb4, A2 and A4 have the characteristics of the genus Bacillus and have rod-shaped characteristics and classified as gram negative. Then isolate A1, A3, B1. It has similar characters to members of the coccus genus, namely round cells and is classified as gram positive, but the isolate code is classified as gram negative. The results of biochemical analysis show that samama plants have 3 types of bacteria, namely Bacillus altitudinis, Bacillus pumilus and Bradyrhizobium sp.

KEBERHASILAN TUMBUH TANAMAN GAHARU (Aquilaria malaccensis) ASAL PROVENANSI AMBON DAN LAIMU DI DESA HATUSUA, KECAMATAN KAIRATU, SERAM BAGIAN BARAT Wattimena, Lenny Stien; Matinahoru, Johan; Tetelay, Febian
MARSEGU : Jurnal Sains dan Teknologi Vol. 1 No. 9 (2024): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/1.9.2024.974-991

Provenance testing is one of the tree breeding approaches to obtain quality seeds, a testing process is needed to find better seed sources in the development area. In this case the species chosen is Agarwood (Aquilaria malaccensis). The aim of this research is to understand the differences in growth of two provenances of gaharu plants (Aquilaria malaccensis) from Ambon Island Village and Laimu Village, Seram Island. This research took place in December 2022 and was carried out in the 2 year old Agarwood Plant Genetic Resources Area (Aquilaria malaccensis), in Hatusua Village, Kairatu District, West Seram Regency. Research shows that of 400 agarwood plants originating from Passo Village, only 309 survived and 91 died, so the percentage of surviving plants reached 77%. Meanwhile, for Laimu Village, of the same 400 plants, there are 315 that are still alive and 85 that are dead, so the percentage of living plants in Laimu Village is 79%. Based on this analysis, the calculated t-value is 2.16, while the t-table at α = 0.05 with 39 degrees of freedom is 2.02. This shows that the t-count is greater than the t-table, which indicates there is a significant difference between the provenances of Passo Village and Laimu Village. For plant diameter, the t-count was recorded at -0.21, and when compared with the t-table at α = 0.05 and 39 degrees of freedom which is 2.02, the t-count is smaller than the t-table. Therefore, there is no difference in diameter between the two provenances.

PEMBIAKAN JAMUR Fusarium oxysforum SEBAGAI MEDIA INOKULASI GAHARU (Aquilaria malaccensis) Sugianto, Puput Irlan Sari; Matinahoru, Johan; Watimena, Cornelia M. A.
MARSEGU : Jurnal Sains dan Teknologi Vol. 2 No. 2 (2025): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/2.2.2025.132-145

This study aims to determine the effect of media on the growth of fungi fusarium oxysforum and Fusarium oxysforum fungal attack on the stem of the Aloes. The research was conducted through 2 stages, namely the breeding stage conducted at the silviculture Laboratory of the Forestry Department and the application stage conducted in Ariate Village, Huamual District, West Seram Regency. The research time is 6 months from January-September 2024. This study used a complete randomized design (RAL), consisting of 1 treatment with 4 levels of inoculants, with 3 repetitions, each treatment A1=inoculant ( potato water and white sugar ), A2= inoculant ( potato water and brown sugar), A3= inoculant (rice water and white sugar), A4= inoculant (rice water and brown sugar). This study used ANOVA to test the effect of the treatment given to the growth and development of fusarium oxysforum fungi . The results of this study showed that the treatment of a1,a2,a3 and A4 can be used as a medium for the growth of fungal colonies fusarium oxysforum. the 4 growing media treatments did not give a significant effect on the speed of colony growth speed of spread, attack length, attack width, color and aroma of clover on plants.

PENGARUH BAKTERI ENDOFIT ASAL AKAR KAYU KETAPANG HUTAN (Terminalia catappa) TERHADAP PERTUMBUHAN DAN PRODUKSI PADI SAWAH (Oryza sativa) Ambarwati, Ririn; Matinahoru, Johan; Hadijah, Miranda
MARSEGU : Jurnal Sains dan Teknologi Vol. 2 No. 4 (2025): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/2.4.2025.235-247

This study aimed to determine the effect of biofertilizer containing endophytic bacteria from the roots of Indian almond trees (Terminalia catappa) on the growth and yield of lowland rice (Oryza sativa). The experiment used a completely randomized design (CRD) with three treatments: NPK fertilizer, endophytic bacterial biofertilizer, and a combination of both, each with three replications. Observed parameters included root dry weight, dry grain weight, and dry rice weight. The results showed that the endophytic bacterial treatment significantly increased all measured parameters compared to NPK and the combination treatments. Treatment A2 (endophytic bacteria) produced the highest values across all variables. These findings indicate that endophytic bacteria from the roots of Terminalia catappa have great potential as a biofertilizer to support sustainable and environmentally friendly rice cultivation.

PENGARUH BAKTERI ENDOFIT ASAL POHON BERHABITAT BASAH TERHADAP PERTUMBUHAN DAN PRODUKTIVITAS PADI SAWAH (Oryza sativa) Nawawi, Mutia; Matinahoru, Johan; Hadijah, Miranda
MARSEGU : Jurnal Sains dan Teknologi Vol. 2 No. 5 (2025): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/2.5.2025.301-315

This study focused on the effect of endophytic bacteria from trees with wet habitats on the growth and productivity of lowland rice (Oryza sativa). Endophytic bacteria were obtained from root extracts of three plant species, namely kayu burung (bird's wood), forest catangensis (katapang hutan), and kayu marsegu (marsegu wood). Samples of the roots of kayu burung (bird's wood) and kayu burung (bird's wood) were collected from Ambon Island, while the roots of katapang hutan and kayu burung (bird's wood) came from Seram Island. The study used a descriptive method with field observations. Parameters observed included plant height, number of leaves, and dry grain weight per stem. The results showed that the highest average plant height was obtained in the treatment of endophytic bacteria from kayu burung (14.16 cm), followed by kayu marsegu (12.16 cm) and katapang hutan (10.16 cm). The treatment of endophytic bacteria containing Monococcus, Streptococcus, and Staphylococcus had a positive effect on vegetative growth and rice production. Endophytic bacteria from marsegu wood and forest catangensis produced the highest dry grain weight, namely 1.216 g per stem. These results demonstrate the potential of endophytic bacteria from forest plants as biological agents to increase rice productivity in lowland rice.

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