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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Malikussaleh Mengabdi
Mariyani
Pelita Mas College of Pharmaceutical Sciences
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Penyuluhan Pemilihan dan Penggunaan Kosmetik yang Aman Tanpa Bahan Kimia Berbahaya Terhadap Remaja M, Mariyani; Patala, Recky; Pratiwi, Dinda
Jurnal Malikussaleh Mengabdi Vol. 2 No. 1 (2023): Jurnal Malikussaleh Mengabdi, April 2023
Publisher : LPPM Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/jmm.v2i1.10662

Abstract

Kosmetik digunakan dengan tujuan memperbaiki maupun merawat kecantikan untuk menunjang penampilan  menjadi suatu kebutuhan primer untuk saat ini khususnya di kalangan remaja. Perkembangan trend kosmetik saat ini menjadi beberapa ancaman dikarenakan ada beberapa produsen yang menambahkan zat yang dilarang dalam penambahan kosmetik demi memperoleh hasil yang instan tanpa memikirkan efeknya, sehingga dilakukan pendampingan agar masyarakat memahai tentang cara pemilihan dan  penggunaan kosmetik yang aman serta dampak akibat penggunaan kosmetik dengan bahan kimia berbahaya. Kegiatan ini menggunakan metode penyuluhan dan  media leaflet, hkegiatan ini menghasilkan pemahaman masyarakat menjadi meningkat mengenai kosmetik. Diharapkan masyarakat remaja Desa Lampo Kabupaten Donggala memiliki pengetahuan dan bijak dalam memilih dan menggunakan kosmetik. 
Analysis Pork Contamination in Beef Meatballs through Polymerase Chain Reaction in Palu City Tresya J. Polibaon; Mariyani; Joni Tandi; Tien Wahyu Handayani; Rahmawati Salsa Dinurrosifa; Ikhsan Hi. Amir Sene
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.9006

Abstract

The need for halal products is essential, particularly among various processed beef products like meatballs, which are popular among nearly the entire community. These products are susceptible to issues of meat counterfeiting for greater economic gain. This study aims to determine the smallest DNA concentration that can still be detected and to assess whether there is any pork contamination in meatballs circulating in Palu City using Polymerase Chain Reaction (PCR). This test uses four DNA samples obtained from fresh meat to test the specificity of the primer: wild boar, rat, and goat DNA samples as comparisons, and pig DNA as a positive control. The presence of DNA bands in the agarose gel electrophoresis visualization results indicates that DNA amplification has occurred, meaning that the sample can be detected. The analysis results showed that the primer used (Cyt-b) had good specificity for detecting pork DNA, while the absolute pork DNA concentration of 0.01 ng/µL and the reference meatball DNA with a concentration of 0.01% are the smallest limits that can still be detected using PCR. Of the four meatball samples from Palu City, one sample produced a DNA band, indicating that the sample contains or is contaminated with pig DNA. This method is valid as it meets the specificity test and demonstrates a good detection limit.
Co-Authors Ikhsan Hi. Amir Sene Joni Tandi Pratiwi, Dinda Rahmawati Salsa Dinurrosifa Recky Patala Tien Wahyu Handayani Tresya J. Polibaon