Article Per Year (5 Year)
p-Index From 2020 - 2025
3.386
P-Index
This Author published in this journals
All Journal Jurnal Natur Indonesia Jurnal Ilmu Perairan (Aquatic Science) Jurnal Akuakultur SEBATIN Jurnal Perikanan dan Kelautan Jurnal Akuakultur SEBATIN Jurnal Natur Indonesia
Iesje Lukistyowati
Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics

Published : 22 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 22 Documents
Search

 1   2   3 
Antibacterial Activity Test of Cinnamomum burmannii Solution in Inhibiting the Growth of Aeromonas hydrophila Bacteria Lilis Cahaya Septiana; Iesje Lukistyowati; Morina Riauwaty
Jurnal Akuakultur SEBATIN Vol. 5 No. 1 (2024): Mei
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.1.1-10

Abstract

Aeromonas hydrophila bacteria that cause MAS (Motile Aeromonas Septicemia) disease. Cinnamon contains essential oils, tannins, saponins, flavonoids and phenols. The active compounds of cinnamon essential oil were cinnamaldehyde and cinnamic acid that can inhibit the growth of bacteria. The research was carried out from March to July 2022 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau, Pekanbaru. This study aimed to determine the sensitivity of Cinnamomum burmannii in inhibiting the growth of Aeromonas hydrophila bacteria, Minimum Inhibitory Concentration (MIC), and Lethal Dose (LD50) concentration of C. burmannii solution against Cyprinus carpio by immersion technique for 24 hours. The research method used was an experimental method using the Kirby Bauer disc method with concentrations of 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, and 1%. The results shown that the sensitivity of the C. burmannii solution to A. hydrophila bacteria ranged from 22.60 mm to 6.67 mm. The MIC value at a concentration of 4% was determined as the average number of bacterial colonies 143 CFU/mL. The LD50 value of C. burmannii solution against Cyprinus carpio by immersion for 24 hours was 3.64%. Based on the data abtain, it can be concluded that C.burmannii is able to inhibit the growth of A.hydrophila bacteria
Prevention of Motile Aeromonas Septicemia Disease with (Muntingia calabura L) to Striped Catfish (Pangasianodon hypophthalmus) Hafil Kurnia Tama; Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 5 No. 1 (2024): Mei
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.1.73-83

Abstract

This research was held from December 2020 – February 2021 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine Science, Universitas Riau. The purpose of this study was to analyze and obtain the best dose of cherry leaf solution to prevent Motile Aeromonas Septicemia from total erythrocytes, hematocrit and hemoglobin levels of striped catfish (Pangasianodon hypophthalmus) fish. The method used to experiment with Completely Randomized Design (CRD) is one factor of five treatment levels. The doses used were negative control without soaking the cherry leaf solution and not tested against Aeromonas hydrophila, positive control without soaking the cherry leaf solution and tested against A. hydrophila, soaking the cherry leaf solution with a dose of 2800 ppm (P1), 3,200 ppm (P2), and 3,600 ppm (P3). Immersion was performed five times for 5 minutes with an interval of 7 days. The results showed that soaking with cherry leaf solution at a dose of 3,600 ppm (P3) was the best dose (P<0.05) with a total value of erythrocytes after the challenge test of 209,66 x 104 cells/mm3, hematocrit level of 37%, hemoglobin 11.86 g/dL, and the survival rate of the test fish was 86,67%.
Isolation of Lactic Acid Bacteria from Intestines of Eel (Monopterus albus) Raised in Biofloc System Cultivation Feri Julianto; Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 4 No. 2 (2023): November
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.2.31-41

Abstract

Biofloc technology is one of the most profitable alternative solutions to the problem of aquaculture waste. The research was carried out from July to August 2020 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine Affairs, Riau University. The purpose of this study was to isolate and identify candidate lactic acid bacteria (LAB) that have the potential as probiotics from the intestines Monopterus albus and to determine the growth rate of candidate probiotic bacteria and can suppress the growth of the pathogenic bacteria Aeromonas hydrophila. The method used is the experimental method with treatment of pH 2, pH 4 and pH 6, isolation was carried out from the best Monopterus albus rearing with a stocking density of 30 fish/25 liters of water. Candidate probiotic bacteria was found from the intestines of Monopterus albus as many as 6 LAB candidate isolates based on the results of physical, biochemical and pH treatments, the morphology of the probiotic candidate isolates consisted of one type of bacteria, namely Lactobacillus, from the 6 isolates which showed the widest zone of inhibition against the pathogenic bacteria Aeromonas hydropila was isolate derived from Monopterus albus intestine result shown that which grows at pH 2 is BL 2.1.1 with an inhibition zone diameter of 9,05 mm.
Identification of Clinostomum sp. Parasites In Swamp Sepat Fish (Trichogaster trichopterus) In FAPERIKA DAM, Pekanbaru Ahmad Tirmizi Nasution; Iesje Lukistyowati; Morina Riauwaty
Jurnal Akuakultur SEBATIN Vol. 4 No. 1 (2023): Mei
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.1.49-57

Abstract

Parasites are organisms that live on other organisms that can benefit from the symbiosis, while the host is harmed. This research was conducted from March to Mei 2022 at the Laboratory of Fish Parasites and Diseases, Department of Aquaculture, Faculty of Fisheries and Marine. Trichogaster trichopterus sampling was carried out Feperika Dam, Pekanbaru. The aim of the study was to obtain the prevalence and intensity of the parasite Clinostomum sp. which infects the swamp fish (Trichogaster trichopterus) in Faperika Dam, Pekanbaru. The method used is a survey method and direct observation of the sample. Sampling was carried out three times with sampling points consisting of 3 places, namely: Location 1 is at the entrance of the reservoir, locations 2 and 3 are located to the west and north of location 1 with a distance of each location 50 meters. The results of the identification showed that the sample of bog fish infected with trematode parasites was Clinostomum sp. with prevalence ranging from 93.9-100%, prevalence in locations 1 and 2 (100%), and intensity for each location, namely, at location 1 at 36.9 ind/bird, location 2 at 41.3 ind and location 3 is 34.3 ind.
Effect of Feeding Containing Hydro Vac Vaccine on The Total Red Blood Cell of Siamese Jambal Fish (Pangasius hypophthalmus) Kept in Cages Nikmal Pane; Morina Riauwaty; Iesje Lukistyowati
Jurnal Akuakultur SEBATIN Vol. 2 No. 1 (2021): May
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.2.1.32-43

Abstract

search was to determine the effect of feeding containing Hydrovac vaccine on the total of red blood cells in Siamese jambal fish (Pangasius hypophthalmus). This research was conducted in December 2019 – January 2020. The method was an experimental method with a Completely Randomized Design (CRD) one factor with four treatments level and three times replication, The treatments were P0: control (feed that does not contain HydroVac vaccine), P1: feed containing HydroVac at a dose 2 ml of vaccine, P2: feed containing HydroVac at a dose 3 ml of vaccine, P3: feed containing HydroVac at a dose 4 ml of vaccine, each treatment dose was diluted with 100 ml distilled water/Kg of feed. The test fish used were the Siamese jambal fish seeds size 8-12 cm. The result shown that the total erythrocyte, hematocryte value, hemoglobin level, absolute weight growth, relative percent survival (RPS) and water quality. The research showed that the best treatment was the giving of vaccine with dose of 4 ml seen from the average eritrocite rate 166.6969x104 sel/mm3, hematocrit value 37.43%, hemoglobin 8.66 g/dL, relatif percent survival (RPS) 56,25 %, beyond life 95,33 % and water quality, temperature range between 28-290C, pH 6, DO 7 and ammonia 0,05 ppm.
Effectiveness of Feed Feeding Containing Fermented Papaya Leaves (Carica papaya L.) Solutions on The Appearance of Erythrocyte and The Growth of Jambal Siam Fish (Pangasianodon hypophthalmus) Latifah Hannum Lase; Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 3 No. 1 (2022): May
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.3.1.63-77

Abstract

Papaya leaves (Carica papaya L.) contain compounds such as tannins, flavonoids, tocophenols, carpain alkaloids, and papain enzymes which have proteolytic activity, antimicrobial and can be used as an immunomodulators that can strengthen the immune system. The purpose of this study was to obtain the best dose of adding fermented papaya leaf solution to feed to improve the health of jambal siam fish (P. hypophthalmus). The method used is an experimental method using a one-factor Completely Randomized Design (CRD) with five treatment levels and three replications. The treatments used were Kn (Negative Control): The feed was not added with fermented papaya leaf solution and was not tested against Aeromonas hydrophila, Kp (Positive Control): The feed was not added with fermented papaya leaf solution but was tested against A. hydrophila), and feed added with fermented papaya leaf solution at a dose of P1 (16 ml/kg), P2 (18 ml/kg), P3 (20 ml/kg) and challenged with A. hydrophila bacteria. The fish were kept for 46 days. The challenge test of A. hydrophila was carried out on the 32nd day intramuscularly with a density of 108 CFU/ml with a dose of 0.1 ml/head. The results showed that the best dose of fermented papaya leaves added to the feed was found in P3 (20 ml/kg) treatment with a total erythrocyte of 284,33 x 104 cells/mm3, the hematocrit value was 38.67%, and the hemoglobin level was 10.67 g/dL, with weight growth absolute 7.80 g. Water quality ranges from 27 – 30 oC, pH 5.5 – 7, DO 4.3 – 5.8 ppm, and ammonia 0.017 – 0.023 mg/L.
Description of Leukocytes of Jambal Siam (Pangasianodon hypophthalmus) Feed Containing Papaya Leaf Solution (Carica papaya L.) Fermented Dian Puspitowati; Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 3 No. 1 (2022): May
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.3.1.78-92

Abstract

Papaya leaves (Carica papaya L.) contain tannins, alkaloids, flavonoids, terpenoids, saponins, and karpain alkaloids which are antiseptic, anti-inflammatory, antifungal, antibacterial and can be used as immunomodulators that can strengthen the immune system. This study aimed to obtain the best dose of fermented papaya leaf (Carica papaya L.) solution in increasing the non-specific immune response of jambal siam fish by looking at total leukocytes, leukocyte differentiation, phagocytic activity, and survival. The method used is an experimental method using a completely randomized design (CRD) with five levels of treatment, namely (Kn): feeding without being given a fermented papaya leaf solution and without being challenged with A. hydrophila bacteria, (Kp): feeding without being given a leaf solution fermented papaya and challenged with A. hydrophila bacteria, and feed added with fermented papaya leaf solution with doses of P1 (16 ml/kg), P2 (18 ml/kg), P3 (20 ml/kg) and challenged with bacteria A. hydrophila. Fish were reared for 46 days. A. hydrophila challenge test was performed on the 32nd day intramuscularly with a density of 108 CFU/ml as much as 0.1 ml/head. The results showed that fermented papaya leaf solution at a dose of 20 mL/kg of feed was the best dose to increase the non-specific immune response of Jambal Siamese fish with an average total leukocyte of 10.05 x104 cells/mm3, lymphocytes 82.00%, monocytes 4 ,67%, neutrophils 6.67%, platelets 6.67%, phagocytic activity 33.67%, and 96,67% survival
Gill histopathology of African Catfish (Clarias gariepinus) that were fed with turmeric and infected with Aeromonas hydrophila Bri Antoni Manalu; Morina Riauwaty; Iesje Lukistyowati
Jurnal Akuakultur SEBATIN Vol. 3 No. 2 (2022): November
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.3.2.14-22

Abstract

Turmeric is an herbal plant that contains natural antibiotics and does not cause residue or danger when consumed. The active substances contained in turmeric are curcumin and essential oils. Curcumin has anti-oxidative, anti-inflammatory and antiseptic effects, turmeric has healing activity, has anti-microbial, anti-fungal activity, essential oil components, turmeric and kurlon have a broad spectrum of power in killing bacteria. Turmeric also has high antioxidant properties because it contains curcumin compounds. Aeromonas hydrophila bacteria often cause disease outbreaks with a high mortality rate (80-100%) in a short time (1-2 weeks). This bacterium causes the fish's body color to darken, the eyes are damaged and appear bulging, the scales are peeling off, all the fins are damaged, red and whitish in color, swimming gasping for air on the water surface, the gills are damaged making it difficult to breathe, the skin becomes rough and appears followed by sores. - ulcers, flatulence. This research aims to determine changes in the structure of the gill tissue of African catfish (Clarias gariepinus) due to being fed a diet containing turmeric (Curcuma domestica) and being infected with Aeromonas hydrophila. This research was conducted from May to June 2021 at the BVet Bukittinggi and at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University. The treatment given was giving turmeric to the feed, namely 0.5 g/kg (P1), 0.7 g/kg (P2), 0.9 g/kg (P3). The results showed that changes in tissue structure that occurred in the gills were hyperplasia, elevated epithelium, edema and deformation of the lamellae. Based on the Histopathological Alteration Index (HAI) the best results were obtained, namely the treatment of giving turmeric 0.9 g/kg (P3) where the HAI score was 1.67 the fish gills were in normal condition, the abnormalities were still mild and could be cured
Identification of Pathogenic Bacteria from Striped Catfish (Pangasionodon hypophthalmus) kept in Aquaculture Ponds Jaya Maruli Tamba; Henni Syawal; Iesje Lukistyowati
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.40-46

Abstract

Koto Masjid Village is the largest production center in striped catfish fish farming activities in Riau Province. One of the inhibiting factors is the disease caused by bacteria, this study aims to determine the types of pathogenic bacteria that have the potential to infect striped catfish. This research was conducted in August - November 2019, samples of striped catfish used in the study amounted to 15 tails with an average length of 15-25 cm taken from 5 ponds in Kampung II, Koto Masjid Village, XIII Koto Kampar Subdistrict, and then identified bacteria by Conventional Laboratory in Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University, Pekanbaru. This study uses a survey method that is by taking samples from the field in Pu and then the isolation of bacteria is carried out from the kidney organ in the TSB media then purified on the TSA media. The results of the study found 3 types of pathogenic bacteria namely Aeromonas sp., Edwardsiella sp., and Pseudomonas sp. Water quality during the study was pH 6.6-70, DO 4.05-4.35 mg/L, NH3 0.035-0.2 mg/L and Temperature 28-300C.
Prevention of Motile Aeromonas Septiceamia in Striped Catfish (Pangasianodon hypophthalmus) using Cherry Leaf Solution Riski Mutia Sari; Iesje Lukistyowati; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 27 No. 1 (2022): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.1.24-31

Abstract

This research was conducted in December 2019 – February 2020 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau. The purpose of this study was to analyze and obtain the best dose of cherry leaf (Muntingia calabura) solution to prevent Motile Aeromonas Septicemia from total leukocytes, leukocyte differentiation, and phagocytic activity of striped catfish (Pangasianodon hypophthalmus). The method used is an experiment with a completely randomized design (CRD) one factor five levels of treatment. The doses used were negative control (without immersion cherry leaf solution and not infection with Aeromonas hydrophila), positive control (without immersion cherry leaf solution and infection with A.hydrophila), P1 (Immersion in cherry leaf solution at a dose of 2800 ppm), P2 (3,200 ppm), and P3 (3,600 ppm). Immersion was carried out 5 times for 5 minutes with an interval of 7 days. The results showed that immersion with cherry leaf solution at a dose of 3,600 ppm (P3) was the best dose (P<0.05) with a total leukocyte value of 10.66 x 104 cells/mm3, lymphocytes 84.67%, monocytes 5.67%, neutrophils 9.67%, phagocytic activity 36.67%, and survival rate 86.67%
Co-Authors Ade Hidayat Ilka Ahmad Tirmizi Nasution Bri Antoni Manalu Dharga Mufti Jr Dian Puspitowati Feri Julianto Hafil Kurnia Tama Henni Syawal Isna Sudirman Jaya Maruli Tamba Latifah Hannum Lase Lilis Cahaya Septiana M Riswan M. Riswan Morina Riauwaty Muhammad Muhajir Nikmal Pane Nurilam Sinaga Nurkholis Ahmad Riski Mutia Sari