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Antibacterial Activity Test of Cinnamomum burmannii Solution in Inhibiting the Growth of Aeromonas hydrophila Bacteria Lilis Cahaya Septiana; Iesje Lukistyowati; Morina Riauwaty
Jurnal Akuakultur SEBATIN Vol. 5 No. 1 (2024): Mei
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.1.1-10

Abstract

Aeromonas hydrophila bacteria that cause MAS (Motile Aeromonas Septicemia) disease. Cinnamon contains essential oils, tannins, saponins, flavonoids and phenols. The active compounds of cinnamon essential oil were cinnamaldehyde and cinnamic acid that can inhibit the growth of bacteria. The research was carried out from March to July 2022 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau, Pekanbaru. This study aimed to determine the sensitivity of Cinnamomum burmannii in inhibiting the growth of Aeromonas hydrophila bacteria, Minimum Inhibitory Concentration (MIC), and Lethal Dose (LD50) concentration of C. burmannii solution against Cyprinus carpio by immersion technique for 24 hours. The research method used was an experimental method using the Kirby Bauer disc method with concentrations of 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, and 1%. The results shown that the sensitivity of the C. burmannii solution to A. hydrophila bacteria ranged from 22.60 mm to 6.67 mm. The MIC value at a concentration of 4% was determined as the average number of bacterial colonies 143 CFU/mL. The LD50 value of C. burmannii solution against Cyprinus carpio by immersion for 24 hours was 3.64%. Based on the data abtain, it can be concluded that C.burmannii is able to inhibit the growth of A.hydrophila bacteria
Identification of Clinostomum sp. Parasites In Swamp Sepat Fish (Trichogaster trichopterus) In FAPERIKA DAM, Pekanbaru Ahmad Tirmizi Nasution; Iesje Lukistyowati; Morina Riauwaty
Jurnal Akuakultur SEBATIN Vol. 4 No. 1 (2023): Mei
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.1.49-57

Abstract

Parasites are organisms that live on other organisms that can benefit from the symbiosis, while the host is harmed. This research was conducted from March to Mei 2022 at the Laboratory of Fish Parasites and Diseases, Department of Aquaculture, Faculty of Fisheries and Marine. Trichogaster trichopterus sampling was carried out Feperika Dam, Pekanbaru. The aim of the study was to obtain the prevalence and intensity of the parasite Clinostomum sp. which infects the swamp fish (Trichogaster trichopterus) in Faperika Dam, Pekanbaru. The method used is a survey method and direct observation of the sample. Sampling was carried out three times with sampling points consisting of 3 places, namely: Location 1 is at the entrance of the reservoir, locations 2 and 3 are located to the west and north of location 1 with a distance of each location 50 meters. The results of the identification showed that the sample of bog fish infected with trematode parasites was Clinostomum sp. with prevalence ranging from 93.9-100%, prevalence in locations 1 and 2 (100%), and intensity for each location, namely, at location 1 at 36.9 ind/bird, location 2 at 41.3 ind and location 3 is 34.3 ind.
Effect of Feeding Containing Hydro Vac Vaccine on The Total Red Blood Cell of Siamese Jambal Fish (Pangasius hypophthalmus) Kept in Cages Nikmal Pane; Morina Riauwaty; Iesje Lukistyowati
Jurnal Akuakultur SEBATIN Vol. 2 No. 1 (2021): May
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.2.1.32-43

Abstract

search was to determine the effect of feeding containing Hydrovac vaccine on the total of red blood cells in Siamese jambal fish (Pangasius hypophthalmus). This research was conducted in December 2019 – January 2020. The method was an experimental method with a Completely Randomized Design (CRD) one factor with four treatments level and three times replication, The treatments were P0: control (feed that does not contain HydroVac vaccine), P1: feed containing HydroVac at a dose 2 ml of vaccine, P2: feed containing HydroVac at a dose 3 ml of vaccine, P3: feed containing HydroVac at a dose 4 ml of vaccine, each treatment dose was diluted with 100 ml distilled water/Kg of feed. The test fish used were the Siamese jambal fish seeds size 8-12 cm. The result shown that the total erythrocyte, hematocryte value, hemoglobin level, absolute weight growth, relative percent survival (RPS) and water quality. The research showed that the best treatment was the giving of vaccine with dose of 4 ml seen from the average eritrocite rate 166.6969x104 sel/mm3, hematocrit value 37.43%, hemoglobin 8.66 g/dL, relatif percent survival (RPS) 56,25 %, beyond life 95,33 % and water quality, temperature range between 28-290C, pH 6, DO 7 and ammonia 0,05 ppm.
Gill histopathology of African Catfish (Clarias gariepinus) that were fed with turmeric and infected with Aeromonas hydrophila Bri Antoni Manalu; Morina Riauwaty; Iesje Lukistyowati
Jurnal Akuakultur SEBATIN Vol. 3 No. 2 (2022): November
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.3.2.14-22

Abstract

Turmeric is an herbal plant that contains natural antibiotics and does not cause residue or danger when consumed. The active substances contained in turmeric are curcumin and essential oils. Curcumin has anti-oxidative, anti-inflammatory and antiseptic effects, turmeric has healing activity, has anti-microbial, anti-fungal activity, essential oil components, turmeric and kurlon have a broad spectrum of power in killing bacteria. Turmeric also has high antioxidant properties because it contains curcumin compounds. Aeromonas hydrophila bacteria often cause disease outbreaks with a high mortality rate (80-100%) in a short time (1-2 weeks). This bacterium causes the fish's body color to darken, the eyes are damaged and appear bulging, the scales are peeling off, all the fins are damaged, red and whitish in color, swimming gasping for air on the water surface, the gills are damaged making it difficult to breathe, the skin becomes rough and appears followed by sores. - ulcers, flatulence. This research aims to determine changes in the structure of the gill tissue of African catfish (Clarias gariepinus) due to being fed a diet containing turmeric (Curcuma domestica) and being infected with Aeromonas hydrophila. This research was conducted from May to June 2021 at the BVet Bukittinggi and at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University. The treatment given was giving turmeric to the feed, namely 0.5 g/kg (P1), 0.7 g/kg (P2), 0.9 g/kg (P3). The results showed that changes in tissue structure that occurred in the gills were hyperplasia, elevated epithelium, edema and deformation of the lamellae. Based on the Histopathological Alteration Index (HAI) the best results were obtained, namely the treatment of giving turmeric 0.9 g/kg (P3) where the HAI score was 1.67 the fish gills were in normal condition, the abnormalities were still mild and could be cured
Sensitivity of Terminalia catappa L. Toward Aeromonas hydrophila Andre Manusun Purba; Morina Riauwaty; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 25 No. 2 (2020): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.2.166-122

Abstract

Terminalia catappa is commonly present in tropical area and its can be used for increasing pH in fish culture and it also be used as disinfectant. The aimed of this study was to obtain a dose of T. catappa leaf solution which was able to inhibit the growth of Aeromonas hydrophila. This research was conducted from April to August 2019, at the Laboratory of Parasites and Fish Diseases, Department of Aquaculture, Faculty of Fisheries and Marine, Riau University. The method used in this study is an experimental method, namely disk diffusion KIRBY-BAUER to see the sensitivity of T. catappa leaf solution against A. hydrophila. The Kirby-Bauer method uses a 6 mm diameter blank disk, to reduce the level of error, then it is repeated three times. The treatments applied were K: Control (Oxytetracycline) at a dose of 30 µg / disk, P1: Giving T. catappa leaf solution 100% dose, P2 (90%), P3 (80%), P4 (70%), P5 (60%) , P6 (50%), P7 (40%), P8 (30%), P9 (20%), P10 (10%) P11 (9%), P12 (8%), P13 (7%), P14 (6%), P15 (5%), P16 (4%), P17 (3%), P18 (2%), P19 (1%). The results showed that the T. catappa leaf solution was able to inhibit the growth of A. hydrophila bacteria at doses of 100-2% with inhibitory zone diameters of 13.9-6.5 mm.
Hematology and Physiology of Striped Catfish (Pangasianodon hypophthalmus) with the addition of guava leaf extract (Psidium guajava L) Ilham Fajri Pohan; Morina Riauwaty; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 29 No. 3 (2024): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.29.3.367-374

Abstract

Striped catfish (Pangasianodon hypophthalmus) is a widely cultivated type of superior food fish. Intensive cultivation of catfish can cause disease. Feeding striped catfish with guava leaf extract is expected to reduce stress, which can be seen in striped catfish's hematological and physiological conditions. This research aims to obtain a dose of guava leaf extract (Psidium guajava L) to improve the health of striped catfish. The method used in the study was an experimental method that applied a one-factor, Completely Randomized Design (CRD) with five treatment levels. The treatment used was without adding guava leaf extract (P0); P1, P2, P3, and P4 with doses of 25, 50, 75, and 100 mL/kg feed, and all treatments were tested against Aeromonas hydrophila. The fish used in this research were 250 striped catfish seeds measuring 6-8 cm and kept for 45 days. Feeding with the addition of guava leaf extract at a dose of 75 mL/kg of feed had an influence on the hematological and physiological profile of post-challenge catfish, such as total erythrocytes of 2.40x106 cells/mm3, hematocrit 35.66%, hemoglobin levels 9.61 g/dL, total leukocytes 10.54x 104 cells/mm3, blood glucose levels of 78.00 mg/dL and phagocytic activity 35.66%. Absolute weight growth 17.76 g, feed efficiency 41.07%.
Histopathology of Striped Catfish (Pangasianodon hypophthalmus) Fed with Moringa Leaf Addition and Maintained in Photoperiod Aquaponics Zulmi Arnoli; Windarti Windarti; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 29 No. 3 (2024): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.29.3.375-380

Abstract

Moringa is one of the plants with high nutrients and antioxidants and can improve fish's immune system. This study aimed to look at the structure of the skin and kidney tissue of striped catfish fed with feed containing Moringa leaf flour after being tested for Aeromonas hydrophila bacteria. This research was conducted from January to May 2024 at the Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau, and fish histology observations were made at the Aquatic Biology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. The method used in this study is an experimental method by applying a one-factor Completely Randomized Design (CRD) with five levels of treatment and three replicates, namely KN (without moringa leaves and A. hydrophila challenge test), KP (without moringa leaves and challenged with A. hydrophila), P1 (dose of 10 g/kg feed), P2 (dose of 15 g/kg feed), P3 (dose of 20 g/kg feed). The results showed that most organ tissues were still in a normal state. The best dose of moringa flour addition to feed is 10 g/kg feed (P1). Fish tissue structure in P1 has damage such as hypertrophy, hemorrhage, and lipofuscin pigment on fish skin. Fish survival during the study was 97.67%.
Identification of Pathogenic Bacteria in Cyprinus carpio Reared in Floating Cages Erikson Sahala Pardamean; Henni Syawal; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.26-32

Abstract

Pathogenic bacteria is disease causing microorganisms that can attack fish and can cause mass death in cultured fish. The purpose of this study was to identify the types of pathogenic bacteria Cyprinus carpio that were reared in floating cages. This study used a survey method that is purposive sampling and the fish sample were obtained from, PLTA Koto Panjang. the fishes were identified in the Fish Parasite and Disease Laboratory, Fish and Marine Faculty, University of Riau. Fish samples used were 15-20 cm goldfish totaling 12 tails with 3 times taken from 4 different cages. The kidneys organ were examined and to find out the type the bacteria. Result shown identified of pathogenic bacteria consist of 3 types of bacteria namely Aeromonas sp., Pseudomonas sp., and Edwardsiella sp. Water quality during the study was Temperature: 27-300C, pH 6-7, DO 3.67-4.28 ppm, and ammonia 0.048-0.64 mg/L.
Description of Erythrocytes of Striped Catfish (Pangasionodon hypophthalmus) Infected by Edwardsiella tarda Treated with Mango Leaf (Mangifera indica) Mariana Lumban Tungkup; Henni Syawal; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.58-65

Abstract

This research has been carried out from November to December 2019 at the Laboratory of Parasites and Fish Diseases at the Fisheries and Marine Faculty of Riau University. The aim of the study was to obtain the best dose of mango leaf solution for the treatment of striped catfish (P. hypophthalmus) infected with E. tarda, seen from the erythrocyte picture. The method used was an experiment with a Completely Randomized Design (CRD) of a factor of five treatment levels and replications three times. Test fish used were striped catfish measuring 8-12 cm, with a total of 150 fish. The treatments used for treatment were 1000 ppm, 1200 ppm, 1400 ppm, negative controls (not infected and not treated), and positive controls (infected and not treated). Parameters measured and observed among others; total erythrocytes, hemoglobin levels, hematocrit values, erythrocyte cell morphology and survival rate. The results showed that apple mango leaf solution (M. indica) was able to treat conjoined striped catfish infected with E. tarda by Emmersion method. The best treatment of 1200 ppm is characterized by an average total erythrocyte, mean total erythrocytes 149.33 x 104 cells / mm3, hemoglobin level 9.03 g / dL, hematocrit value 35.67% and the survival of the test fish after treatment is 86.67%. The results of water quality measurements during the study, namely temperature 27.00-27.46 0C, pH 6.1-6.77, dissolved oxygen 3.53-3.87 mg/L, and ammonia 0.02 mg/L.
Histopathology of Kidney of Striped Catfish (Pangasiodon hypophthalmus) Feed with Turmeric Flour Sri Wahyuni; Morina Riauwaty; Windarti Windarti
Jurnal Perikanan dan Kelautan Vol. 25 No. 3 (2020): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/232-237

Abstract

Turmeric is an herbal plant that has antibacterial and antioxidant abilities, and also to improve the fish's immune system. The aim of this research was to look at the histological structure of kidney and survival rate fish which was given feed with turmeric flour before and after infected with A. hydrophila. This research was conducted from February to May 2019 in the Laboratory of Parasites and Fish Diseases of the Faculty of Fisheries and Marine Sciences, University of Riau. The method in this research was the experimental method by one-factor Completely Randomized Design (CRD) with five levels. Kn (feeding without given turmeric flour and without infection with A. hydrophila), Kp (Feeding without given turmeric flour and infected with A. hydrophila), P1 (turmeric flour at a dose of 0.5 g / kg of feed), P2 (dose of 0.7 g / kg), P3 (dose of 0.9 g / kg). The results showed that the histological structure of catfish which was given the addition of turmeric flour in feed by normal conditions and after being challenged with A. hydrophila showed some damage to the gill and kidney structure. The best dose of adding turmeric flour to feed is 0.7 g / kg of feed (P2), seen from the structure of the kidney cell, and the best survival. Histological structure of kidney cell structure in P2 treatment has abnormality such as hemorrhage and survival rate 100%.