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Journal : Journal of Fisheries

Effect of Homogenization Process on the Production of Arthrospira platensis Carotenoid Nanocapsules Encapsulated with Arabic Gum and Whey Protein Concentrate Khusnul Alfionita; Siti Ari Budhiyanti; Nurfitri Ekantari
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i1.32029

Abstract

Highlight ResearchThe homogenization process affected the particle size reductionThe homogenization process using both High Speed Homogenizer and Ultrasound had a smaller particle size and the highest efficiency in encapsulation AbstractArthrospira platensis contains a high concentration of carotenoids mainly 80 % β-carotene. The use of β-carotene in functional food and nutritional supplements was constrained by its lack of chemical and physical stability. Therefore, efforts were needed to protect carotenoids from damage by using an encapsulation system. The emulsion preparation before the encapsulation process had an influence on the stability of the oil-in-water emulsion. Various methods were used to prepare emulsions, such as high shear homogenizers (HSH) and ultrasound (US) treatment. The homogenization step was critical because it influenced the chemical stability of any encapsulated compounds. The purpose of this work was to generate nanocapsules of β-carotene with minimum carotenoid degradation, high efficiency of encapsulation, and small particle size, with variation in the homogenization process. This study consisted of 5 treatments, including the homogenization processes used HSH 24.000 rpm for 90 s ; HSH 24.000 rpm for 60 s, amplitude 55 % for 120 s ; amplitude 94 % for 138 s ;  amplitude 55 % for 138 s ;  and  amplitude 55 % for 120 s . Each parameter was analysed by ANOVA followed by Tukey pairwise comparisons with 95% confidence level and p<0.05. The particle size was influenced by the emulsification process, such as homogenization treatment, homogenization time, amplitude, and time of sonication. The sample that was homogenized using both HSH and US had a smaller particle size and the highest efficiency in encapsulation than others. The combination of homogenization process could decrease particle size.
Response Surface Methodology for Optimized Concentration of Gum Arabic, Maltodextrin, and Whey Protein Isolate in Arthrospira platensis Phycocyanin Microcapsules Pebriyanti, Puspa; Budhiyanti, Siti Ari; Ekantari, Nurfitri
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.74555

Abstract

Graphical Abstract   Highlight Research Phycocyanin could be encapsulated by the spray-drying process with gum Arabic (GA), maltodextrin (MD), and whey protein isolate (WPI) as coating materials. The selected optimal formula for phycocyanin microcapsules consisted of 8.3% GA, 11.7% MD, and 5.2% WPI. The optimum formulation can produce phycocyanin microcapsules meeting 76.56% of the desired targets. The concentrations of three encapsulant ingredients (GA, MD, and WPI) influenced the resulting characteristics, e.g., phycocyanin content, antioxidant activity, encapsulation efficiency, phycocyanin retention, solubility, and particle size   Abstract Phycocyanin is a blue-colored phycobiliprotein in Arthrospira platensis known for its antioxidant properties. Due to its sensitivity to pH, temperature, light, oxygen, and moisture, protecting phycocyanin pigments often involves microencapsulation through spray drying. This process allows the pigments to be rapidly entrapped within a wall material. The wall material for these microcapsules was composed of gum arabic, maltodextrin, and whey protein isolate. This study aimed to determine the optimal concentrations of these components to optimize encapsulation performance. Optimization was performed using the Minitab application with Response Surface Methodology (RSM) and a Central Composite Design (CCD). The independent variables were the concentrations of gum arabic, maltodextrin, and whey protein isolate, while the response variables measured included yield, phycocyanin content, antioxidant activity, encapsulation efficiency, phycocyanin retention, solubility, and particle size. Scanning electron microscopy (SEM) was used to analyze the morphology of the optimized microcapsules. The Minitab analysis recommended 20 potential optimization solutions, with the highest desirability value of 0.7656. The selected optimal formula consisted of 8.3% gum arabic, 11.7% maltodextrin, and 5.2% whey protein isolate. Predicted response values for this formulation were as follows: yield 75.30%, phycocyanin content 4.55%, antioxidant activity 48.87%, encapsulation efficiency 98.98%, phycocyanin retention 68.57%, solubility 95.15%, and particle size 212.73 nm. Validation results confirmed a yield of 81.70%, phycocyanin content of 3.56%, antioxidant activity of 52.08%, encapsulation efficiency of 93.96%, phycocyanin retention of 62.42%, and a particle size of 212.73 nm. These findings indicate that the proposed solution is both effective and acceptable.