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Antibacterial and antibiofilm activity of mint leaves (Mentha piperita L) extracts against Streptococcus mutans UA159: a laboratory experiment Muhtar, Azmi Nur Azizah; Atthoriq, Adriansyah Altaf; Jeffrey, Jeffrey; Djohan, Frita Ferlita Shafri; Soerachman, Badi
Padjadjaran Journal of Dentistry Vol 36, No 1 (2024): March 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no1.52744

Introduction: Oral health is a major concern in healthcare worldwide, with dental caries being a prevalent issue among children and adults. Streptococcus mutans is a primary bacterium implicated in the development of dental caries due to its acidogenic nature. Mint leaf (Mentha piperita L.) is a unique herbal plant that has antibacterial and antibiofilm properties against S. mutans and minimal side effects. The purpose of this study was to analyze the antibacterial effectiveness of mint leaf against the growth of S. mutans UA159 and its antibiofilm effects. Methods: This study was conducted experimentally with the posttest-only control group design, using the broth microdilution method in 6 test groups, namely mint leaf extract with a concentration of 3.125, 6.25, 12.5, 25, 50, and 100% and also two control groups, namely the negative control using aquadest and the positive control using 0.2% chlorhexidine. Biofilm growth is determined by comparing Optical Density (OD) values and then calculating the percentage of eradication of S. mutans biofilm formation. Results: The results indicated that the mint leaf extract exhibited antibacterial effects against S. mutans, with the largest inhibition zone diameter observed at a 100% concentration. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)90 values using the broth microdilution method in this study were 12.5% and 100% concentrations, respectively. Mint leaf extract has been shown to significantly inhibit the growth of S. mutans (p<0.05). The results of the inhibition test on biofilm formation at a concentration of 100% averaged 87.39%. Conclusion: Mint leaf extract has an antibacterial and antibiofilm effect on the growth of S. mutans. The level of inhibition of mint leaf extract on the growth of S. mutans is proportional to its concentration; the higher the concentration of the extract, the stronger the inhibitory ability.KeywordAnti-Bacterial agents, biofilms, Mentha piperita, oral health, Streptococcus mutans.

Effectiveness of Sage Leaf Gel on Neutrophil Count in Wound Healing Meliawaty, Florence; Djohan, Frita Ferlita Shafri; Ramdhani, Muhamad Syahrul
Global Medical & Health Communication (GMHC) Vol 13, No 1 (2025)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v13i1.14302

A wound involves damage to the epithelial layer of the skin, extending to the subcutis and surrounding tissues. The body initiates a healing process starting with inflammation. Neutrophils play a crucial role in this phase; excessive neutrophils can cause tissue necrosis, while insufficient neutrophils may lead to infection. Neutrophils are essential for bacterial eradication during inflammation. Sage leaf extract, known for its anti-inflammatory, antioxidant, and antibacterial properties, may aid in wound healing. This study evaluates the effectiveness of 5% sage leaf extract gel on neutrophil count during the wound healing process in Wistar rats. This study was conducted in several Universitas Jenderal Achmad Yani Cimahi laboratories from 29 May 2023 to 23 February 2024. A post-test-only control group design was used with 25 samples divided into five groups: (K-1) no treatment, tissue sampled at 13 minutes; (K-2) no treatment, tissue sampled at 103 minutes; (KN) healthy/normal control; (KP1) 5% sage leaf extract gel, tissue sampled at 13 minutes; (KP2) 5% sage leaf extract gel, tissue sampled at 103 minutes. Data analysis was performed using the Kruskal-Wallis test followed by the Mann-Whitney post hoc test (p≤0.05), indicating a statistically significant difference. The highest mean neutrophil count was observed in the KP2 group, which received 5% sage leaf extract gel and had tissue sampled at 103 minutes. The study concludes that a 5% sage leaf extract gel is effective in increasing neutrophil counts during the healing of punch wounds in Wistar rats, which contributes to accelerated wound healing.

Effectiveness of Sage Leaf Gel on Neutrophil Count in Wound Healing Meliawaty, Florence; Djohan, Frita Ferlita Shafri; Ramdhani, Muhamad Syahrul
Global Medical & Health Communication (GMHC) Vol 13, No 1 (2025)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v13i1.14139

A wound involves damage to the epithelial layer of the skin, extending to the subcutis and surrounding tissues. The body initiates a healing process starting with inflammation. Neutrophils play a crucial role in this phase; excessive neutrophils can cause tissue necrosis, while insufficient neutrophils may lead to infection. Neutrophils are essential for bacterial eradication during inflammation. Sage leaf extract, known for its anti-inflammatory, antioxidant, and antibacterial properties, may aid in wound healing. This study evaluates the effectiveness of 5% sage leaf extract gel on neutrophil count during the wound healing process in Wistar rats. This study was conducted in several Universitas Jenderal Achmad Yani Cimahi laboratories from 29 May 2023 to 23 February 2024. A post-test-only control group design was used with 25 samples divided into five groups: (K-1) no treatment, tissue sampled at 13 minutes; (K-2) no treatment, tissue sampled at 103 minutes; (KN) healthy/normal control; (KP1) 5% sage leaf extract gel, tissue sampled at 13 minutes; (KP2) 5% sage leaf extract gel, tissue sampled at 103 minutes. Data analysis was performed using the Kruskal-Wallis test followed by the Mann-Whitney post hoc test (p≤0.05), indicating a statistically significant difference. The highest mean neutrophil count was observed in the KP2 group, which received 5% sage leaf extract gel and had tissue sampled at 103 minutes. The study concludes that a 5% sage leaf extract gel is effective in increasing neutrophil counts during the healing of punch wounds in Wistar rats, which contributes to accelerated wound healing.

Hubungan teknik menyikat gigi horizontal scrub murni dan horizontal scrub kombinasi dengan kejadian resesi gingiva pada pra-lansia dan lansia di kota cimahi: Studi cross-sectional Mubaraq, Rizky Anzal; Djohan, Frita Ferlita Shafri
Padjadjaran Journal of Dental Researchers and Students Vol 10, No 2 (2026): June 2026
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v10i2.70058

ABSTRAKPendahuluan: Menyikat gigi merupakan salah satu metode membersihkan gigi, namun jika dilakukan dengan teknik yang keliru dapat mengakibatkan resesi gingiva. Teknik menyikat gigi Horizontal scrub adalah teknik yang sederhana tetapi memiliki potensi menyebabkan resesi gingiva. Sebagian individu menyikat gigi dengan teknik Horizontal scrub kombinasi. Informasi penelitian yang menjelaskan hubungan teknik horizontal scrub murni dan Horizontal scrub kombinasi terhadap kejadian resesi gingiva masih terbatas. Penelitian ini memiliki tujuan untuk menganalisis hubungan teknik menyikat gigi Horizontal scrub murni dan horizontal scrub kombinasi terhadap kejadian resesi gingiva pada pra-lansia dan lansia di Kota Cimahi Metode: Desain penelitian yaitu analitik observasional cross-sectional. Subjek penelitian adalah pra-lansia dan lansia pada tiga kecamatan di Kota Cimahi sebanyak 165 orang. Analisis korelasi antara teknik menyikat gigi dengan resesi gingiva dengan uji Fisher dengan p-value (p<0,05) (OR<1 dan CI=95%). Hasil: Prevalensi resesi gingiva pada kelompok horizontal scrub murni sebesar 97,7% lebih tinggi dibandingkan kelompok Horizontal scrub kombinasi sebesar 82,9%. Secara statistik menunjukkan terdapat hubungan signifikan antara teknik menyikat gigi dengan kejadian resesi gingiva (p=0,003). Subjek yang menggunakan teknik horizontal scrub murni memiliki resiko lebih tinggi mengalami resesi gingiva dibandingkan kelompok horizontal scrub kombinasi (OR= 0,114; 95%CI: 0,027-0,484) Simpulan: Terdapat hubungan bermakna antara teknik menyikat gigi Horizontal scrub murni dan Horizontal scrub kombinasi dengan kejadian resesi gingiva (p=0,003; OR=0,114; 95% CI: 0,027-0,484). Perlu penguatan kembali edukasi teknik menyikat gigi pada pasien 45 tahun ke atas sebagai upaya pencegahan resesi gingiva.KATA KUNCI: Horizontal scrub; menyikat gigi; resesi gingivaThe association between pure and combined horizontal scrub technique and gingival recession among pre-elderly and elderly individuals in cimahi city: Study cross-sectional ABSTRACT Introduction: Toothbrushing is one of the most common methods of maintaining oral hygiene, however, when performed incorrectly, it may lead to gingival recession. The horizontal scrub technique is simple and widely used, but it may pose a risk of gingival recession. In practice, some individuals apply the horizontal scrub technique in combination with other brushing movements. Information regarding the association between pure horizontal scrub and combined horizontal scrub toothbrushing techniques and the occurrence of gingival recession remains limited. This study aimed to analyze the association between pure horizontal scrub and combined horizontal scrub toothbrushing techniques and the occurrence of gingival recession among pre-elderly and elderly individuals in Cimahi City. Methods: This study employed an observational analytic cross-sectional design. The study subjects were pre-elderly and elderly individuals from three sub-districts in Cimahi City, with a total of 165 participants. The association between toothbrushing technique and gingival recession was analyzed using Fisher’s Exact Test with a significance level of p<0.05 and 95% confidence interval. Results: The prevalence of gingival recession in the pure horizontal scrub technique group (97.7%) was higher than that in the combined horizontal scrub technique group (82.9%). Statistical analysis revealed a significant association between toothbrushing technique and the occurrence of gingival recession (p=0.003). Subjects using the pure horizontal scrub technique were at a significantly higher risk of developing gingival recession compared to those using the combined horizontal scrub technique (OR=0.114; 95% CI: 0.027-0.484) Conclusions: There was a significant association between the pure horizontal scrub technique and combined horizontal scrub toothbrushing techniques of gingival recession. Reinforcement of toothbrushing technique education for patients aged 45 years and older is needed as an effort to prevent gingival recession.KEY WORDS: Horizontal scrub; toothbrushing; gingival recession

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