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Temperature and holding time of instrument sterilization as an infection control of odontectomy Meliawaty, Florence; Mangundjaja, Sunardhi; Hardjawinata, Karlina
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Odontectomy should be performed aseptically. The goal of sterilization is the complete killing of all forms of microbial life including bacterial spores on the items being processed. Biologic monitoring provides the main guarantee of sterilization. The aim of this study was to find the interrelation of the temperature and the holding time of instrument sterilization as an infection control for the successful of lower molars odontectomy. This experimental laboratory study was conducted at the Oral Maxillofacial Surgery Department in the Hasan Sadikin General Hospital Bandung and at the Microbiology Laboratory Faculty of Dentistry, Universitas Padjadjaran, Jatinangor. The Protocol was performed in three methods of sterilization: dry heat with oven and ozone, dry heat with oven and infrared (125)C for 15 minutes), both were monitored by Bacillus atrophaeus as the biologic indicators, and autoclavization (121" C for 15 minutes) with Geobaciflus stearothermophilus as the biological monitoring, with 17 times repetition. After sterilization, all of the indicators were cultured on Nutrient Agar Plate (NAPS), and the subsequent growth was assessed. The colony forming units (CFUs) were counted by Stuart Electric Bacteria Colony Counter. Adequate positive and negative controls were used in every cycle. The results showed that after autoclavization, all spores were killed. In comparison with dry heat in the oven, there were still CFUs on the NAPs, but no colonies grow after 3 repetitions by oven and infrared. Heating in oven and ozone could only reduce the spore numbers, even after repeating 5 times. The reduction of the CFUs was greater in more repetition. According to the statistical analysis, the differences were significant. This study concluded that sterilization by oven and infrared will be achieved after 3 holding times (30-35 minutes) and dry heat with oven and ozone could only act as a germicide. In autoclavization, all of Geobacillus steorothermophilus have been killed.

STEVENS JOHNSON SYNDROME DISEBABKAN OLEH DRUG ERUPTION OBAT ANALGETIK Putriani, Wivda; Meliawaty, Florence
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 1 No 1 (2018): Medika Kartika Edisi PIT 10 Kedokteran Gigi
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Stevens Johnson Syndrome adalah satu bentuk reaksi obat pada kulit yang parah, yang ditandai dengan demam dan luka mukokutan yang berlanjut menjadi nekrosis dan epidermis terkelupas.  Laporan kasus ini merupakan tipe observasi di ruangan perawatan rumah sakit, yang disebabkan penggunaan asam mefenamat setelah ekstraksi gigi. Reaksi obat yang parah seperti Stevens Johnson Syndrome dapat berpotensi mengancam jiwa yang memerlukan perawatan ekstra.

Maternal Education Level and Child's Anxiety on Dental Extraction Jeffrey Jeffrey; Florence Meliawaty; Asih Rahaju
Journal of Medicine and Health Vol. 2 No. 1 (2018)
Publisher : Universitas Kristen Maranatha

Dental extraction can cause anxiety in children, observed in vital signs changes. If occursexcessively, it can be regarded as an obstacle and may affect a child's behavior that can furtherdetermine the success of dental treatment. Anxiety that occurs in children can usually be influencedby various factors, including the level of parental education. This study aimed to observe maternaleducation level and anxiety in children receiving dental extraction treatments. This is a descriptivestudy, involving 60 subjects aged 7-12 that went for dental extraction at Rumah Sakit Gigi MulutPendidikan Universitas Jenderal Achmad Yani (RSGMP UNJANI) Cimahi Juli-Desember 2016. Theparameters examined for measuring anxiety are body temperature, respiration frequency, and pulsebefore and after the procedure. This study found that 63.3% of mother of the subject had high schooleducation, 16.7% of junior high school, 8.3% S1, 6.7% D1/D3, and 5% SD. Increased bodytemperature was obtained in 36 subjects, increased respiration frequency in 46 subjects, andincreased pulse in 34 subjects. We concluded that the majority of maternal education levels in thisstudy are high school graduates and the anxiety parameters in the majority of subjects increase whenreceiving dental extraction procedure.Keywords: child's anxiety, dental extraction, mother education level

UJI DAYA ANTIBAKTERI EKSTRAK DAUN SIRIH MERAH (Piper crocatum) TERHADAP BAKTERI Porphyromonas gingivalis SEBAGAI TERAPI PENUNJANG PERIODONTITIS KRONIS Frita Ferlita Shafri Djohan; Florence Meliawaty
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 5 No 3 (2022): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Porphyromonas gingivalis merupakan bakteri patogen dalam penyakit periodontitis kronis. Kandungan senyawa organik daun sirih merah (Piper crocatum) memiliki efek antibakteri. Ekstrak Piper crocatum diharapkan dapat digunakan sebagai terapi penunjang penyakit periodontitis kronis. Penelitian ini bertujuan untuk mengetahui efek antibakteri ekstrak Piper crocatum melalui uji daya hambat terhadap Porphyromonas gingivalis. Prosedur kerja dengan membuat sampel ekstrak etanol 80% daun sirih merah dengan konsentrasi 2,5%; 5%; dan 10% dalam 10 mL DMSO 10%. Kultur biakan bakteri P. gingivalis ATCC 33277 di media Lempeng Agar Darah (LAD). Uji daya hambat dengan metode Kirby Bower pada konsentrasi ekstrak 2.5%, 5%, 10% pengulangan sebanyak tiga kali pada LAD berisi bakteri. Hasil uji daya hambat yaitu tidak didapat zona bening pada konsentrasi ekstrak 2.5%, 5%, 10%. Kontrol positif tidak ada dan kemungkinan bakteri terkontaminasi dapat menjadi penyebab zona bening tidak terbentuk. Ekstrak Piper crocatum pada konsentrasi 2,5%, 5%, dan 10% tidak memiliki efek terhadap bakteri P. gingivalis. DOI : 10.35990/mk.v5n3.p308-320

Analysis of FOXE1 rs4460498 and GSTP-1 I105V associated with non syndromic cleft lip and palate among Deutero Malay Subrace in Indonesia Nasroen, Saskia Lenggogeni; Meliawaty, Florence; Tammama, Tichvy; Prasdiaz, Putri Indriani; Choirunnisa, Tias; Maskoen, Ani Melani
Padjadjaran Journal of Dentistry Vol 34, No 3 (2022): November 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no3.39296

ABSTRACTIntroduction: FOXE1 rs4460498 and GSTP-1 I105V gene polymorphisms are suspected of having a role in some of the non-syndromic cleft lip and palate (NS CLP) populations worldwide. This study aims to analyze FOXE1 rs4460498 and GSTP-1 I105V polymorphisms associated with NS CLP as the risk factor among Deutero Malay Subrace in Indonesia. Methods: This study was a case-control design, using samples from the venous blood of 102 NS CLP subjects and 102 healthy control subjects. After DNA was extracted, the PCR-RFLPs method was performed using TasI restriction enzyme on 100 blood samples of FOXE1 rs4460498 group and Alw26I restriction enzyme on 105 blood samples of the GSTP-1 I105V group. The Chi-Square test was used with the Kolmogorov Smirnov and Exact Fisher alternatives. Results: T mutant allele (OR= 0.926, p>0.05) and CT genotype (OR= 0.0, p>0.05) of FOXE1 rs4460498 and the G mutant allele (OR= 0.988,p>0.05) and AG genotype (OR= 0.675,p>0.05) of the GSTP-1 I105V are not the risks of NS CLP. Conclusion: FOXE1 rs4460498 and GSTP-1 I105V gene polymorphisms are not associated with non-syndromic cleft lip and palate among Deutero Malay Subrace in the Indonesian population.Key words: deutero malay; FOXE1 rs4460498; GSTP-1 I105V; NS CLP

Peningkatan Pengetahuan Masyarakat Kampung Adat Cireundeu Cimahi-Jawa Barat Mengenai Potensi Tanaman untuk Kesehatan Gigi Yuslianti, Euis Reni; Meliawaty, Florence; Sabirin, Indah Puti R.; Juliastuti, Henny; Rakhmat, Iis Inayati; Handayani, Dewi Ratih; Faramayudha, Fahrauk; Riyanti, Soraya; Syam, Akhirul Kahfi; Rahaju, Asih; Suntana, Mutiara Sukma; Khaerunnisa, Rahmadaniah; Adinugraha, Irham M.
Jurnal Medika: Medika Vol. 4 No. 1 (2025)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/53ye5t32

Kesehatan gigi dan mulut merupakan aspek penting dalam kesejahteraan masyarakat, namun masih sering kurang mendapatkan perhatian, terutama di komunitas adat yang memiliki keterbatasan akses terhadap layanan kesehatan. Padahal, alam menyediakan berbagai tanaman yang memiliki potensi sebagai agen perawatan gigi dan mulut, seperti yang ditemukan di Kampung Adat Cireundeu, Cimahi. Pengabdian masyarakat ini bertujuan untuk meningkatkan pengetahuan masyarakat mengenai manfaat tanaman termasuk daun reundeu bagi kesehatan gigi dan mulut melalui penyuluhan dan pemeriksaan kesehatan gigi. Metode pengabdian masyarakat berupa penyuluhan interaktif dengan partisipasi warga mengenai tanaman berkhasiat antioksidan, antimikroba, antiinflamasi, dan kaya nutrisi, serta pemeriksaan kesehatan gigi untuk menilai kondisi gigi warga Kampung Adat Cireundeu. Evaluasi dilakukan melalui pretes dan postes terhadap 46 warga sebelum dan setelah penyuluhan Hasil menunjukkan adanya peningkatan pengetahuan masyarakat tentang manfaat tanaman termasuk daun reundeu tuntuk kesehatan gigi. Selain itu, kegiatan ini membantu dalam identifikasi masalah kesehatan gigi yang dihadapi warga. Kesimpulan penyuluhan terbukti efektif dalam meningkatkan pemahaman masyarakat mengenai potensi tanaman untuk kesehatan gigi. Sebagai langkah lanjutan, diperlukan program edukasi berkelanjutan dan pendampingan dalam pemanfaatan tanaman secara mandiri guna menjaga kesehatan gigi dan mulut secara optimal.

Maternal Education Level and Child's Anxiety on Dental Extraction Jeffrey Jeffrey; Florence Meliawaty; Asih Rahaju
Journal of Medicine and Health Vol 2 No 1 (2018)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28932/jmh.v2i1.738

Dental extraction can cause anxiety in children, observed in vital signs changes. If occursexcessively, it can be regarded as an obstacle and may affect a child's behavior that can furtherdetermine the success of dental treatment. Anxiety that occurs in children can usually be influencedby various factors, including the level of parental education. This study aimed to observe maternaleducation level and anxiety in children receiving dental extraction treatments. This is a descriptivestudy, involving 60 subjects aged 7-12 that went for dental extraction at Rumah Sakit Gigi MulutPendidikan Universitas Jenderal Achmad Yani (RSGMP UNJANI) Cimahi Juli-Desember 2016. Theparameters examined for measuring anxiety are body temperature, respiration frequency, and pulsebefore and after the procedure. This study found that 63.3% of mother of the subject had high schooleducation, 16.7% of junior high school, 8.3% S1, 6.7% D1/D3, and 5% SD. Increased bodytemperature was obtained in 36 subjects, increased respiration frequency in 46 subjects, andincreased pulse in 34 subjects. We concluded that the majority of maternal education levels in thisstudy are high school graduates and the anxiety parameters in the majority of subjects increase whenreceiving dental extraction procedure.Keywords: child's anxiety, dental extraction, mother education level

Effectiveness of Sage Leaf Gel on Neutrophil Count in Wound Healing Meliawaty, Florence; Djohan, Frita Ferlita Shafri; Ramdhani, Muhamad Syahrul
Global Medical & Health Communication (GMHC) Vol 13, No 1 (2025)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v13i1.14302

A wound involves damage to the epithelial layer of the skin, extending to the subcutis and surrounding tissues. The body initiates a healing process starting with inflammation. Neutrophils play a crucial role in this phase; excessive neutrophils can cause tissue necrosis, while insufficient neutrophils may lead to infection. Neutrophils are essential for bacterial eradication during inflammation. Sage leaf extract, known for its anti-inflammatory, antioxidant, and antibacterial properties, may aid in wound healing. This study evaluates the effectiveness of 5% sage leaf extract gel on neutrophil count during the wound healing process in Wistar rats. This study was conducted in several Universitas Jenderal Achmad Yani Cimahi laboratories from 29 May 2023 to 23 February 2024. A post-test-only control group design was used with 25 samples divided into five groups: (K-1) no treatment, tissue sampled at 13 minutes; (K-2) no treatment, tissue sampled at 103 minutes; (KN) healthy/normal control; (KP1) 5% sage leaf extract gel, tissue sampled at 13 minutes; (KP2) 5% sage leaf extract gel, tissue sampled at 103 minutes. Data analysis was performed using the Kruskal-Wallis test followed by the Mann-Whitney post hoc test (p≤0.05), indicating a statistically significant difference. The highest mean neutrophil count was observed in the KP2 group, which received 5% sage leaf extract gel and had tissue sampled at 103 minutes. The study concludes that a 5% sage leaf extract gel is effective in increasing neutrophil counts during the healing of punch wounds in Wistar rats, which contributes to accelerated wound healing.

Penyuluhan Kesehatan Gigi Mulut untuk Meningkatkan Pengetahuan Keluarga Pasien Celah Bibir dan Langit-Langit sebagai Implementasi Program Merdeka Belajar Kampus Merdeka Nasroen, Saskia Lenggogeni; Yuslianti, Euis Reni; Meliawaty, Florence; Ardan, Rachman; Isnaeni, Rheni Safira; Irsyad, Widya; Irfianti, Isti
Jurnal Abdimas Kartika Wijayakusuma Vol 3 No 1 (2022): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v3i1.172

Masyarakat yang keluarganya mengalami kondisi bibir sumbing atau celah bibir dan langit-langit mayoritas berasal dari kalangan ekonomi lemah. Kelainan bawaan yang bermanifestasi dari bibir atas ke gusi, dan langit-langit yang tidak sepenuhnya menyatu atau berkembang secara terpisah yang dapat disebabkan oleh faktor genetik dan lingkungan. Keluarga yang memiliki riwayat dengan celah bibir dan langit-langit penting mengetahui pengetahuan tentang celah bibir dan langit-langit. Kegiatan pengabdian masyarakat ini dilakukan sebagai implementasi program Merdeka Belajar Kampus Merdeka (MBKM) dosen dan mahasiswa Fakultas Kedokteran Gigi Unjani untuk membantu masyarakat luar kampus yang tidak terjangkau edukasi dari pelayanan kesehatan dengan intensif. Selain itu dilakukan penelitian dengan metode kuesioner kepada para orang tua penderita di Yayasan Pembina Penderita Celah Bibir dan Langit-langit Bandung. Data dianalisis T-test dependent (p<0,05). Hasil menunjukkan terdapat pengaruh signifikan terhadap peningkatan pengetahuan setelah penyuluhan dibandingkan dengan sebelum penyuluhan pada keluarga pasien celah bibir dan langit-langit (p=0,00). Dapat disimpulkan bahwa program MBKM yang dilaksanakan dapat berperan pada perubahan pengetahuan keluarga mengenai kesehatan gigi dan mulut pasien dengan riwayat celah bibir dan langit-langit setelah dilakukannya penyuluhan.

Effectiveness of Sage Leaf Gel on Neutrophil Count in Wound Healing Meliawaty, Florence; Djohan, Frita Ferlita Shafri; Ramdhani, Muhamad Syahrul
Global Medical & Health Communication (GMHC) Vol 13, No 1 (2025)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v13i1.14139

A wound involves damage to the epithelial layer of the skin, extending to the subcutis and surrounding tissues. The body initiates a healing process starting with inflammation. Neutrophils play a crucial role in this phase; excessive neutrophils can cause tissue necrosis, while insufficient neutrophils may lead to infection. Neutrophils are essential for bacterial eradication during inflammation. Sage leaf extract, known for its anti-inflammatory, antioxidant, and antibacterial properties, may aid in wound healing. This study evaluates the effectiveness of 5% sage leaf extract gel on neutrophil count during the wound healing process in Wistar rats. This study was conducted in several Universitas Jenderal Achmad Yani Cimahi laboratories from 29 May 2023 to 23 February 2024. A post-test-only control group design was used with 25 samples divided into five groups: (K-1) no treatment, tissue sampled at 13 minutes; (K-2) no treatment, tissue sampled at 103 minutes; (KN) healthy/normal control; (KP1) 5% sage leaf extract gel, tissue sampled at 13 minutes; (KP2) 5% sage leaf extract gel, tissue sampled at 103 minutes. Data analysis was performed using the Kruskal-Wallis test followed by the Mann-Whitney post hoc test (p≤0.05), indicating a statistically significant difference. The highest mean neutrophil count was observed in the KP2 group, which received 5% sage leaf extract gel and had tissue sampled at 103 minutes. The study concludes that a 5% sage leaf extract gel is effective in increasing neutrophil counts during the healing of punch wounds in Wistar rats, which contributes to accelerated wound healing.

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