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All Journal Biomedical Journal of Indonesia
Mita Al Maida
Medical Education Program, Faculty of Medicine, Universitas Sriwijaya-Dr. Mohammad Hoesin Hospital, Palembang, Indonesia
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Comparative Evaluation of Electrochemiluminescence and Chemiluminescence Microparticle Immunoassays for Anti-Hepatitis C Virus (HCV) Detection Phey Liana; Mita Al Maida; Gita Dwi Prasasty; Desi Oktariana; Tungki Pratama Umar
Biomedical Journal of Indonesia Vol. 10 No. 3 (2024): Vol 10, No 3, 2024
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v10i3.208

Abstract

Introduction. Anti-hepatitis C virus (HCV) testing is an immunological analysis designed to identify the presence of antibodies against the HCV antigen. This investigation is typically conducted using the chemiluminescence immunoassay (CLIA) technique, which yields precise results. Current research attempts to evaluate the outcomes of the anti-HCV test utilizing the Chemiluminescence Microparticle Immunoassay (CMIA) and the Electrochemiluminescence Immunoassay (ECLIA) approaches. Methods. This cross-sectional study comprised 63 serum samples collected via consecutive sampling. The acquired data were subjected to statistical analysis utilizing Cohen's Kappa agreement test. Results. Both immunoassay methods yielded identical results, indicating four reactive samples out of 63, equating to 6.35%. The agreement test result for the anti-HCV test was κ=1.000, signifying an almost perfect level of agreement. Conclusion. The anti-HCV assessment utilizing CMIA and ECLIA methodologies demonstrated near-perfect agreement. This signifies that these two procedures can be employed in clinical laboratories concurrently or interchangeably for the test.
Co-Authors Desi Oktariana Gita Dwi Prasasty Phey Liana Tungki Pratama Umar