Lating, Muhammad Fajri Aditthia
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Essential Oil Components of Clove Raja Variant Flower Bud Oil (Syzygium aromaticum (L.) Merr. & L. M. Perry) in Mamala Village, Central Maluku Regency, Maluku Province Mahulette, Asri Subkhan; Lating, Muhammad Fajri Aditthia; Wattimena, Anna Yuliana; Makaruku, Marlita Herlin
Jurnal Agrohut Vol. 15 No. 2 (2024): Agrohut
Publisher : Fakultas Pertanian Universitas Darussalam Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51135/agh.v15i2.339

Abstract

Clove Raja is classified as an intermediate clove type because it has morphological characteristics similar to cultivated-type cloves from the aromatic group and wild-type cloves from the non-aromatic group. The morphological similarity indicates similarities in the essential oil components between Raja clove, cultivated, and wild-type cloves. The study aims to identify the essential oil components of one of the Raja clove variants by comparing them with the results of previous essential oil component analysis in cultivated type cloves from the aromatic group represented by Tuni cloves and wild type cloves from the non-aromatic group represented by Hutan cloves. The material for distillation comes from the flower part of one of the Raja clove variants. Raja clove flower oil distillation was carried out using the steam-hydro distillation method. In contrast, the equipment for analyzing essential components used Gas Chromatography-Mass Spectrometry (GC-MS) in the form of Agilent Technologies 7890. The critical component data obtained were analyzed using R Stat 3.1.0 software to produce a heatmap profile of essential oil grouping. The results of the Gas Chromatography-Mass Spectrometry (GC-MS) analysis obtained seven crucial components of Raja cloves, namely eugenol (75.35%); caryophyllene (9.63%); Germacrene-D (3.73%); I-Isopropyl-4,7-dimethyl-1,2,3,5,6,8a—hexahydronaphtalene (1.65%); humulene (1.36%); 2'-3',4' trimethocyacetophenone (1.22%); α-cubebene (1%). The characteristic components of Raja clove oil in the heatmap profile are indicated as eugenol; germacrene-D; α-humulene; 2'-3',4' Trimethocyacetophenone, I-isopropyl-4,7-dimethyl-1,2,3,5,6,8a-hexahydronaphtalene. The identification results show that Raja cloves have similarities in the main component of eugenol with the results of previous studies on cultivated cloves represented by Tuni cloves, while the similarity to wild-type cloves represented by Hutan cloves is in the form of  germacrene-D.
Morphological Character of Clove “Raja” (Syzygium aromaticum L.), Endemic to Maluku, Indonesia Wattimena, Anna Yuliana; Mahulette, Asri Subkhan; Makaruku, Marlita Herlin; Lating, Muhammad Fajri Aditthia
Journal of Tropical Crop Science Vol. 10 No. 01 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.1.71-78

Abstract

Clove “Raja” is one of the local Maluku clove germplasm, which has characteristics similar to cultivated cloves and wild types. Until now, very limited information that are available on the morphological character of clove “Raja”. This study aims to provide basic information on the morphological diversity of “Raja”. The study was conducted at the location of the distribution of cloves “Raja” in Mamala village, Leihitu sub-district, Central Maluku district, Maluku province, in June-August 2022. The descriptors used referred to Tropical Fruit Descriptors, with some modifications. The clove “Raja” that were characterized belonged to the farmers and consisted of 30 accessions of cloves that were over twenty years old. The characterization variables included the characters of trees, stems, branches, leaves, flowers, fruits, and seeds. The results of Hierarchical Cluster Analysis (HCA) on 30 plant samples based on 54 morphological characters obtained two accession groups with a dissimilarity coefficient of 41%. The results of the Principle Component Analysis (PCA) got a total diversity of 70.5% where the first group consisted of accessions of cloves “Raja” group I having identifiable characters in the form of leaf area, leaf length, leaf width, and leaf tip shape; while the second group of clove accessions of “Raja” group I in the form of stem circumference, petiole length, flower length, fruit length, fruit width, and fruit weight. Characteristics of accession of “Raja” cloves group II had distinctive morphological traits in the form of crown shape, upper surface of leaves, lower surface of leaves, leaf thickness, leaf texture, old leaf color, shoot color, leaf tip color, leaf aroma, leaf spiciness, flower stalk length, and seed color.