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All Journal Jurnal Sain Veteriner
Aminudin, Muhammad Rizki
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Veterinary

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Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus Aminudin, Muhammad Rizki; Lukman, Denny Widaya; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.99843

Abstract

Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus.
Detection of ESBL-Encoding Genes in Antibiotic-Resistant Escherichia coli Isolated from Houseflies (Musca domestica) in Campus Food Court Lukman, Denny Widaya; Pisestyani, Herwin; Aminudin, Muhammad Rizki; Supriyono, Supriyono; Sukmawinata, Eddy
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.105866

Abstract

Escherichia coli is a dangerous pathogen that is reported to bring about disease in humans as well as animals. E. coli is commonly associated with diseases due to food contamination. Some strains of the pathogen have shown resistance to various antibiotics, one of them is ESBL-E. coli, leading to complex problems in health. The transmission of pathogenic bacteria in the environment can be mediated by houseflies (Musca domestica). This study aimed to analyze the presence of antibiotic-resistant E. coli bacteria and detect resistance genes carried by houseflies (M. domestica) collected from food courts in IPB University, Dramaga Bogor, Indonesia. The study included isolation and identification of E. coli bacteria based on ISO 16649-2: 2001 standards. E. coliisolates are then tested for antibiotic sensitivity using the Kirby Bauer disc diffusion method based on CLSI in 2023. Antibiotics tested included ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, chloramphenicol, ceftazidime, and cefotaxime. Then, the identification of genes encoding ESBL resistance (blaCTX, blaTEM, and blaSHV) was conducted. The study found 5 isolates (5/40; 12.5%). Total 4 isolates of 5 isolates indicated resistance against two or several antibiotics tested. Detection of genes encoding ESBL resistance in 5 E. coli isolates showed that 2 isolates (40%) were positive for the blaTEM gene but did not show positive results for blaCTX and blaSHV genes. The appearance of antibiotic-resistant E. coli in houseflies in food court in campus should be taken into account. 
Co-Authors Denny Widaya Lukman Herwin Pisestyani Mirnawati Bachrum Sudarwanto Sukmawinata, Eddy Supriyono Supriyono