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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner
Sukmawinata, Eddy
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Veterinary

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Escherichia coli Penghasil Extended-spectrum Β-Lactamases yang Diisolasi pada Peternakan Broiler-Ikan Terintegrasi Prasaja, Aditya Gilang; Wulandari, Eka; Anggaeni, Trianing Tyas Kusuma; Sukmawinata, Eddy
Acta VETERINARIA Indonesiana Vol. 12 No. 1 (2024): Maret 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.1.25-31

Abstract

Extended-spectrum β-lactamases (ESBL) adalah sekelompok enzim yang diproduksi oleh bakteri untuk dapat resisten terhadap antibiotika termasuk sefalosporin generasi ketiga yang menjadi masalah kesehatan baik pada hewan dan manusia. Tujuan penelitian ini adalah untuk mengetahui keberadaan bakteri Escherichia coli penghasil ESBL serta profil resistensinya pada komponen lingkungan di sekitar peternakan broiler-ikan terintegrasi. Deteksi E. coli penghasil ESBL dan uji sensitivitas dilakukan dengan uji double disc synergy dan disc diffusion, dan hasil interpretasi mengacu pada The European Committee on Antimicrobial Susceptibility Testing. Sebanyak 19 isolat E. coli penghasil ESBL diisolasi dari sampel swab kloaka, ikan, dan lingkungan pada suatu peternakan broiler-ikan terintegrasi. Secara rinci, E. coli penghasil ESBL terdeteksi pada seluruh sampel sampel air (3/3), sampel ikan (2/2), sampel tanah (1/1), dan 86,7% sampel swab kloaka (13/15). Hasil pengujian kepekaan terhadap antibiotika menunjukkan resistensi terhadap ampisilin (100%), seftriakson (100%), siprofloksasin (68,4%), dan tetrasiklin (42,1%). Selain itu, 8 isolat yang terdiri dari sampel swab (6/13; 46,1%), air (1/3; 33,3%), dan ikan (1/2; 50,0%) teridentifikasi sebagai bakteri multi-drug resistant. Studi ini dapat menggambarkan terjadinya cemaran E. coli penghasil ESBL di lingkungan peternakan broiler-ikan terintegrasi.
Detection of ESBL-Encoding Genes in Antibiotic-Resistant Escherichia coli Isolated from Houseflies (Musca domestica) in Campus Food Court Lukman, Denny Widaya; Pisestyani, Herwin; Aminudin, Muhammad Rizki; Supriyono, Supriyono; Sukmawinata, Eddy
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.105866

Abstract

Escherichia coli is a dangerous pathogen that is reported to bring about disease in humans as well as animals. E. coli is commonly associated with diseases due to food contamination. Some strains of the pathogen have shown resistance to various antibiotics, one of them is ESBL-E. coli, leading to complex problems in health. The transmission of pathogenic bacteria in the environment can be mediated by houseflies (Musca domestica). This study aimed to analyze the presence of antibiotic-resistant E. coli bacteria and detect resistance genes carried by houseflies (M. domestica) collected from food courts in IPB University, Dramaga Bogor, Indonesia. The study included isolation and identification of E. coli bacteria based on ISO 16649-2: 2001 standards. E. coliisolates are then tested for antibiotic sensitivity using the Kirby Bauer disc diffusion method based on CLSI in 2023. Antibiotics tested included ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, chloramphenicol, ceftazidime, and cefotaxime. Then, the identification of genes encoding ESBL resistance (blaCTX, blaTEM, and blaSHV) was conducted. The study found 5 isolates (5/40; 12.5%). Total 4 isolates of 5 isolates indicated resistance against two or several antibiotics tested. Detection of genes encoding ESBL resistance in 5 E. coli isolates showed that 2 isolates (40%) were positive for the blaTEM gene but did not show positive results for blaCTX and blaSHV genes. The appearance of antibiotic-resistant E. coli in houseflies in food court in campus should be taken into account. 
Co-Authors Aminudin, Muhammad Rizki ANGGAENI, TRIANING TYAS KUSUMA Baehaqi Denny Widaya Lukman Herwin Pisestyani Prasaja, Aditya Gilang Supriyono Supriyono