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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner JURNAL KAJIAN VETERINER
Mirnawati Bachrum Sudarwanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health Veterinary

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Praktek Sanitasi Higiene pada Usaha Pengolahan Dangke Susu Sapi di Kabupaten Enrekang, Sulawesi Selatan (THE PRACTICES OF HYGIENIC SANITATION IN PROCESSING INDUSTRIES OF COW MILK DANGKE IN ENREKANG DISTRICT, SOUTH SULAWESI) Wahniyathi Hatta; Mirnawati Bachrum Sudarwanto; Idwan Sudirman; Ratmawati Malaka
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.857 KB)

Abstract

Lack of hygienic sanitation conditions in food processing industry could contribute to the food safety.The aim of this study was to assess the implementation of hygienic sanitation practices in the processingindustries of cow milk dangke in Enrekang District, South Sulawesi. Total of 60 respondents wereinterviewed using questionnaire and observation on hygienic sanitation practices was carried out usingcheck-list.  Respondents were producers and also workers in the processing units in Cendana Sub-district,Enrekang which had been selected with simple random sampling technique.  Scoring of hygienic sanitationcondition were based on the application of hygienic sanitation practices on the checklist.  None of workerswore special clothes, put dangke molds in closed container, and wiped packaging of dangke with specialclothes.  Less than 50% of the workers  wore a head coverings, prepared papaya latex with boiled water,and covered dangke when molding.  A total of 50% of the workers immediately washed the molds afterused.  More than 50% of the workers washed their hands with soap before dangke processing, washed themolds with soap, wiped the packagings with a cloth before it used, stored the milk in a closed container,and cleaned papaya fruit before preparing papaya latex.  All of the workers made dangke while doinghousework. Hygienic sanitation condition of dangke processing industries that had good category was 3%while another 57% in moderate category and poor category was 40%.  The hygienic sanitation practices hadnot been fully implemented on the processing industries of cow milk dangke in Enrekang district, which ledto the category of hygienic sanitation condition of largely of them was moderate .
Kesetaraan Uji Mastitis IPB-1 dengan Metode Breed untuk Mendiagnosis Mastitis Subklinis pada Susu Kerbau Murrah dan Kambing (THE EQUALITY OF IPB-1 MASTITIS TEST WITH BREED METHOD FOR SUB-CLINICAL MASTITIS DETECTION ON MURRAH BUFFALO’S MILK AND GOAT’S MILK Mirnawati Bachrum Sudarwanto; Hera Maheshwari; Faisal Tanjung
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.368 KB)

Abstract

Sub-clinical mastitis causes decrease in milk production and milk quality. It is not only happen tomilking dairies, but also happens to dairy buffalos and goats. The objective of this study is to know thedifferences between IPB-1 mastitis test (IMT) and Breed method to diagnose sub-clinical mastitis ondairy buffaloes and goats. Fourty two samples of buffalo’s milk and 20 samples of goat’s milk were used tosomatic cell count (SCC) with direct and indirect method. Direct method was performed by counting themilk’s SCC with Breed method, and indirect method was performed by observing the reaction betweenIMT reagent and milk. The results showed that 28 from 42 samples (66.67%) of buffalo’s milk and 13 from20 samples (65%) of goat’s milk tested with Breed method came from the herds which suffered from subclinicalmastitis and 27 from 42 samples (64.28%) and 10 from 20 samples (50%) of goat’s milk testedwith IMT showed positive reaction. This research also showed that IMT has sensitivity of 96% and specivicityof 100% for buffalo’s milk and sensitivity of 71% and specivicity of 100% for goat’s milk. IMT can be usedto obtain fast result for sub-clinical mastitis diagnosis and it is faster and easier for buffalo’s and goat’smilk.
Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus Aminudin, Muhammad Rizki; Lukman, Denny Widaya; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.99843

Abstract

Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus.
Multidrug-Resistant (MDR) E. coli dalam Daging Babi yang Berasal dari Rumah Potong Hewan (RPH) Oeba, Kota Kupang Mila Meha, Margie Pristiantine; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Acta VETERINARIA Indonesiana Vol. 12 No. 2 (2024): Juli 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.2.168-175

Abstract

To produce pork ready for consumption, it must go through various stages, from the farm to the packaging. The chances of contamination with pathogenic microbes are very large. Pathogenic microbes are often found in pork, one of which is E. coli, which is often the trigger for foodborne disease in humans. Previous studies have shown that E. coli isolates from pig farms in Kupang City are known to have multidrug resistance (MDR), which, if this event spreads, could negatively impact the community. RPH is a place for slaughtering animals supervised by veterinarians to produce good quality meat. This study aims to measure E. coli contamination, identify and isolate MDR E. coli from fresh pork from Oeba RPH, Kupang City. The enumeration number of E. coli was carried out based on the Indonesian National Standard (SNI) ISO 16649–2:2001 concerning the Horizontal Enumeration Method for E. coli positive-b-glukuronidase - Part 2: Colony counting technique at 44 °C using 5-bromo-4-chloro-3-indolyl-b-D-glucuronide. Resistance test of E. coli to antibiotics was carried out based on the Kirby-Bauer method. Data related to the amount of E. coli contamination and testing of E.coli antibiotic resistance were analyzed descriptively. The results showed that the contamination of E. coli in the pork used had an average of 626,440 ± 947,937 CFU/g. All E. coli isolates were resistant to penicillin (100%), most E. coli isolates were resistant to streptomycin (80%) and tetracycline (60%), and half of the E. coli isolates were resistant to oxytetracycline (47%) and amoxicillin (40%). E. coli isolates obtained from pork had MDR against 2 (7/15;46.67%) to 3 (7/15;46.67%) groups of antibiotics. This study concludes that the contamination of E. coli in pork from Oeba slaughterhouse exceeds the maximum microbial contamination limit (>1x101 CFU/g). E. coli isolates were resistant to penicillin, streptomycin, tetracycline, oxytetracycline, and amoxicillin and had MDR against 2 to 3 classes of antibiotics.
Analisis Fisikokimia dan Aktivitas Antibakteri Madu Hutan Timor terhadap Methicillin-Resistant Staphylococcus aureus Undari, Fajar Okta; Latif, Hadri; Sudarwanto, Mirnawati Bachrum
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.21448

Abstract

The emergence of antibiotic-resistant bacteria has become a global concern. One of the bacteria that is resistant to several types of antibiotics is Methicillin-Resistant Staphylococcus aureus (MRSA). Honey is known for its nutritional and antibacterial properties. The antibacterial activity of honey is due to several factors such as its physicochemical properties, H2O2, and phytochemicals compounds. Honey could be a promising alternative to overcome the problem of antibiotic resistance. The aim of this research was to determine the physicochemical, H2O2, Pinocembrin, Kaempferol and antimicrobial activity of Timor Forest honey. A total of 7 Timor Forest honey was analyzed to determine water content, water activity, pH, H2O2, Pinocembrin, Kaempferol and antibacterial activity. Moisture content, water activity, and pH were measured using a refractometer, aw-meter, and pH meter, respectively. Hydrogen peroxide content was measured using peroxide strip test. Pinocembrin and Kaempferol contents were measured using HPLC-DAD. The analysis showed that the water content, water activity and pH of Timor Forest honey were 17.52±3.31%, 0.6±0.01, 4.18±0.32, respectively. The content of H2O2, Pinocembrin, and Kaempferol were 109.1±47.1 µmol/L, 2.2±1.9 mg/100 g, 1.7±0.7 mg/100 g, respectively. The antibacterial activity of Timor Forest honey against MRSA was tested using Kirby-Bauer disc diffusion method. Vancomycin was used as positive control. The inhibition zone of Timor Forest honey and vancomycin was not significantly different with an average of 13.93±0.23 mm for Timor Forest honey and 14.33±0.05 mm for vancomycin. Timor forest honey has good physicochemical properties with a relatively high content of Pinocembrin and Kaempferol and has antibacterial activity against MRSA.
Co-Authors Aminudin, Muhammad Rizki Denny Widaya Lukman Faisal Tanjung Hadri Latif HERA MAHESHWARI Herwin Pisestyani Idwan Sudirman Mila Meha, Margie Pristiantine Ratmawati Malaka Undari, Fajar Okta Wahniyathi Hatta