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Tis’iyyah , Siti Nur Maghfiroh
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Environmental Science Health Professions Nursing Public Health

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Specific Primer Design of Leucine Rich Repeats and Guanylate Kinase Domain Containing (LRGUK) Genes in Type II Diabetes Mellitus Patients Mushlih, Miftahul; Tis’iyyah , Siti Nur Maghfiroh; Rini, Chylen Setiyo; Sari, Azizah Krismonita
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 6 No 2 (2022): Medical Technology and Public Health Journal September 2022
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v6i2.3476

Abstract

Diabetes Mellitus type II (DT2) is a disorder of insulin function (insulin resistance) caused by 2 factors, i.e. environmental and genetic factors. Previous studies have identified the presence of specific alleles that differentiate between DT2 and non-DT2 sufferers. Identification of the allele indicated leucine rich repeats and guanylate kinase domain containing (LRGUK) gene. The aim of this research was to design a specific primer to amplify LRGUK gene. The primer design was based on a 576 bp nucleotide base and added 100 bp in the 5'  and 100 bp in the 3' direction using NCBI-Primer BLAST. The primers produced were selected based on eight criteria’s. The results were validated with 6 samples of DT2 patients and visualized using agarose gel. The results of the analysis showed that the primers Forward 5'-TCCTACTCTGTGTCCTTCCTTG-3' and Reverse 5'-GTGGTGACAAGGAGG TTTGC-3' were able to amplify specifically with a length of 687 bp.
Co-Authors Chylen Setiyo Rini Miftahul Mushlih Sari, Azizah Krismonita