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All Journal Jurnal Biodjati Journal of Biotechnology and Natural Science Berita Biologi
Wardani, Diah Kartika
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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Journal : Journal of Biotechnology and Natural Science

 1 
Identification of Phytochemical Compounds and Determination of Flavonoid and Tannin Levels in Wedelia (Sphagneticola trilobata (L.) Pruski) Setiawan, Haris; Hidayat, Andika Gundawa; Wardani, Diah Kartika; Makhabati, Ulinuha Farah
Journal of Biotechnology and Natural Science Vol. 5 No. 1 (2025): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v5i1.12349

Abstract

Wedelia plant, which has the scientific name Sphagneticola trilobata (L.) Pruski, is one of the plants classified as weeds and is found in various agricultural areas. The largest phytochemical compound content in wedelia leaves consists of flavonoids, tannins and alkaloids. The purpose of this study was to identify phytochemical compounds contained in wedelia leaves and to determine the levels of existing compounds. This research method consists of wet sorting, making extracts by maceration method using 96% ethanol. The ethanol extract was then analyzed using Fourier Transform InfraRed (FT-IR), High Performance Liquid Chromatography (HPLC), and UV-Vis Spectrophotometer. Data analysis to determine the line equation and correlation value using statistical analysis application SPSS Version 30.0 and analysis of phytochemical compound components using descriptive analysis. Based on the results of the analysis of phytochemical compounds using the FTIR method, several functional groups were obtained that were very identical to antioxidant compounds in the form of flavonoids, tannins, and alkaloids. After that, further tests on flavonoid levels using the HPLC method obtained levels of 215.435 mg/g (%b/b), and determination of tannin levels using a UV-Vis Spectrophotometer obtained levels of 128.35 mg/g (%b/b).
Study of Tridax procumbens L. Extract on SOD and MDA levels in Cingarette Smoke Exposed Rats setiawan, haris; Azali, Aulia Syafadilla; Wardani, Diah Kartika; Nurazizah, Intan Faya; Nurrohiim, Annisaa’
Journal of Biotechnology and Natural Science Vol. 5 No. 2 (2025): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v5i2.12948

Abstract

Cigarette smoke is a source of free radicals that can damage cells and tissues of the respiratory tract. Tridax procumbens L. has potential as an antioxidant because it contains flavonoids that play a role in counteracting free radicals. The study aims to determine the potential of Tridax procumbens L. as a source of antioxidants against SOD and MDA levels in Wistar rats exposed to cigarette smoke. The study used Wistar rats (25 heads) with 5 treatments consisting of K (aquadest), KN (exposed to cigarette smoke), P1 (exposed to cigarette smoke and given 100 mg/kg BW extract), P2 (exposed to cigarette smoke and given 200 mg/kg BW extract), and P3 (exposed to cigarette smoke and given 300 mg/kg BW extract). Extract administration was carried out on days 1-21 and cigarette smoke exposure was carried out on days 8-21. Parameters observed consisted of flavonoid content test (by KLT method, total flavonoid content, total phenolic content), body weight, SOD levels (whole blood method), and MDA levels (TBARs method). Data were analyzed by one-way ANOVA test (P<0.05) followed by Duncan post hoc test. The results showed that Tridax procumbens L. contains flavonoids with an Rf value of 0.23. Body weight showed no significant difference (P>0.05), but SOD and MDA levels were different between treatments (P<0.05) with the most optimum dose of 300 mg/kg BW. The conclusion shows that a dose of 300 mg/kg BW of ethanol extract of Tridax procumbens L. can act as a natural antioxidant from exposure to cigarette smoke in vitro.
Co-Authors AA Sudharmawan, AA Abda Abda Aris Setiawan Azali, Aulia Syafadilla Hidayat, Andika Gundawa Iffatuzzahra', Yumna Nabilah Makhabati, Ulinuha Farah Nurazizah, Intan Faya Nurrohiim, Annisaa’ Nurwijayanti