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All Journal 3BIO: Journal of Biological Science, Technology and Management Narra J
Priharto, Neil
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Enhancing Lipid Extraction from Chlorella vulgaris Microalgae for Biodiesel Production: Application of Natural Deep Eutectic Solvent (NaDES) in Cell Disruption Priharto, Neil; Nadia Yasmin Dicky
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.2

Abstract

Natural deep eutectic solvent (NaDES) pre-treatment offers a promising alternative strategy to enhance lipid extraction efficiency from microalgae by influencing the integrity of the cell wall, thereby improving solvent accessibility to the cytoplasm and facilitating the release of intracellular lipid content. In this study, Chlorella vulgaris biomass underwent pre-treatment with four different NaDES formulations, each based on choline chloride (ChCl) with varying molar ratios: ChCl : Glycerol (1:2), ChCl : Glycerol (1:1), ChCl : Oxalic Acid (1:2), dan ChCl : Oxalic Acid (1:1).We analyzed the impact of these NaDES pre-treatments on lipid yield and fatty acid profiles. The extracted lipids exhibited an acid value of 42.56 mg KOH/g fat and a free fatty acid content of 0.25%. Samples subjected to NaDES treatment showed significant increases in lipid extraction efficiency, with lipid yields ranging from 1.25 to 2.3 times higher than those of untreated biomass extracted using hexane (p < 0.05). The highest lipid yield was observed in samples treated with ChCl : Glycerol (1:1), achieving a total lipid yield of 19.44% (w/w), more than double that of the untreated biomass. Although minor variations in fatty acid profiles were noted due to the NaDES treatment, the dominant fatty acids in each variation remained palmitic acid (C16:0, 21-29.5%) and oleic acid (C18:1, 13-43.88%).
Development of an inactivated viral transport medium for diagnostic testing in low-resource countries Rahmani, Silmi; Meitha, Karlia; Septiani, Popi; Priharto, Neil; Kamarisima, Kamarisima; Ningrum, Ratih A.; Angelina, Marissa; Agustiyanti, Dian F.; Wisnuwardhani, Popi H.; Nugroho, Herjuno A.; Tan, Marselina I.
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2068

Abstract

Viral transport medium (VTM) is crucial for retaining clinical specimens, such as the virus or its genetic material from the mucus of respiratory tract of coronavirus disease 2019 (COVID-19) suspected patients. However, the locally produced VTM in Indonesia lacks the ability to inactivate the virus, risking the safety of diagnostic personnel. The aim of this study was to formulate inactive VTM (iVTM) incorporating chaotropic agents like guanidine salt, along with anionic detergents, chelators, buffers, and surfactants, to inactivate the virus while maintaining RNA integrity. Viral RNA stability in iVTM (pH 4 and pH 6) was evaluated for 30 days at 4°C and 25–28°C. In vitro inactivation test was performed on SARS-CoV-2 isolate (variant B1). The stability test revealed that storing the clinical specimens in iVTM at pH 6 maintained severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) detectability by qPCR for up to 30 days at cold and room temperatures. Stability assessments conducted over a 4-month period (at 25–28°C) on iVTM with a pH of 6 revealed clear appearance, consistent pH stability, no alteration in the solution color, and no indications of bacterial or fungal contamination. Results from an in vitro inactivation assay demonstrated that iVTM pH 6 eliminated SARS-CoV-2 infectivity within just five minutes of contact. These findings suggest that iVTM pH 6 offers a safer and cost-effective alternative for handling and transportation of clinical specimens.
Co-Authors Agustiyanti, Dian F. Kamarisima, Kamarisima Marissa Angelina Meitha, Karlia Nadia Yasmin Dicky Ningrum, Ratih A. Nugroho, Herjuno A. RAHMANI, SILMI Septiani, Popi Tan, Marselina I. Wisnuwardhani, Popi H.