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All Journal Narra J Asia Pacific Journal of Sustainable Agriculture, Food and Energy (APJSAFE)
Septiani, Popi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Energy Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Development of an inactivated viral transport medium for diagnostic testing in low-resource countries Rahmani, Silmi; Meitha, Karlia; Septiani, Popi; Priharto, Neil; Kamarisima, Kamarisima; Ningrum, Ratih A.; Angelina, Marissa; Agustiyanti, Dian F.; Wisnuwardhani, Popi H.; Nugroho, Herjuno A.; Tan, Marselina I.
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2068

Abstract

Viral transport medium (VTM) is crucial for retaining clinical specimens, such as the virus or its genetic material from the mucus of respiratory tract of coronavirus disease 2019 (COVID-19) suspected patients. However, the locally produced VTM in Indonesia lacks the ability to inactivate the virus, risking the safety of diagnostic personnel. The aim of this study was to formulate inactive VTM (iVTM) incorporating chaotropic agents like guanidine salt, along with anionic detergents, chelators, buffers, and surfactants, to inactivate the virus while maintaining RNA integrity. Viral RNA stability in iVTM (pH 4 and pH 6) was evaluated for 30 days at 4°C and 25–28°C. In vitro inactivation test was performed on SARS-CoV-2 isolate (variant B1). The stability test revealed that storing the clinical specimens in iVTM at pH 6 maintained severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) detectability by qPCR for up to 30 days at cold and room temperatures. Stability assessments conducted over a 4-month period (at 25–28°C) on iVTM with a pH of 6 revealed clear appearance, consistent pH stability, no alteration in the solution color, and no indications of bacterial or fungal contamination. Results from an in vitro inactivation assay demonstrated that iVTM pH 6 eliminated SARS-CoV-2 infectivity within just five minutes of contact. These findings suggest that iVTM pH 6 offers a safer and cost-effective alternative for handling and transportation of clinical specimens.
Eco-bioremediation potential of mealworm gut microbiome for polystyrene degradation in nutrient-based media Afandi, Afandi; Septiani, Popi; Fibriani, Azzania
Asia Pacific Journal of Sustainable Agriculture, Food and Energy Vol. 13 No. 2 (2025): December 2025
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy Network (SAFE Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36782/apjsafe.v13i2.535

Abstract

Polystyrene is a petroleum-based polymer widely used in single-use packaging and thermal insulation. Its high molecular weight and structural stability make it highly resistant to degradation, leading to persistent environmental accumulation. Recent studies have highlighted the potential of insect gut microbiota, particularly from mealworms, to biodegrade plastic waste. In this study, gut extracts were inoculated into Nutrient Broth (NB) and Potato Dextrose Broth (PDB), each supplemented with polystyrene as emulsion, powder, or film. NB showed the highest reduction in polystyrene weight (0.93%) by day 28, compared to PDB (0.73%). Total plate count and yeast-mold count analyses revealed microbial proliferation in both media, with NB exhibiting a higher final bacterial count (7.562 log cfu/mL) than PDB (6.510 log cfu/mL), while fungal counts were comparable. Scanning Electron Microscopy confirmed structural damage to polystyrene films, including surface roughening and micro-pitting, especially in NB. These findings indicate that media composition significantly influences microbial growth and degradation efficiency. NB appears to favor bacterial communities with enhanced plastic-degrading capabilities, demonstrating superior biodegradation potential in liquid culture.
Co-Authors Afandi Afandi Agustiyanti, Dian F. Fibriani, Azzania Kamarisima, Kamarisima Marissa Angelina Meitha, Karlia Ningrum, Ratih A. Nugroho, Herjuno A. Priharto, Neil RAHMANI, SILMI Tan, Marselina I. Wisnuwardhani, Popi H.