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All Journal Info Abdi Cendekia Indonesian Journal of Endocrinology Metabolism and Diabetes (InaJEMD)
Sabrina Azmi
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Religion Computer Science & IT Health Professions Library & Information Science Medicine & Pharmacology Public Health Social Sciences

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Edukasi Pemberantasan Sarang Nyamuk (PSN) pada Anak-anak Sekolah Dasar Negeri 14 Pagi Sumur Batu Jakarta Pusat Ferlianti, Rika; Sabrina Azmi; Silpi Hamidiyah
Info Abdi Cendekia Vol. 7 No. 2: Desember 2024
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/iac.v7i2.167

Abstract

Prevalensi Demam Berdarah Dengue (DBD) di Indonesia masih tinggi dan angka kematian tertinggi terjadi pada anak-anak. Salah satu faktor yang mempengaruhi penyebaran penyakit DBD adalah terbatasnya pengetahuan masyarakat tentang pencegahan penyakit. Sekolah merupakan lingkungan yang potensial untuk penyebaran dan penularan penyakit DBD. Peran strategis anak usia sekolah sebagai bagian dari kegiatan pemberdayaan masyarakat dalam pencegahan dan penanggulangan DBD. Anak usia sekolah dapat berperan dalam upaya pemberantasan sarang nyamuk (PSN) baik di sekolah maupun di rumah. Tujuan dari Edukasi Pemberantasan Sarang Nyamuk (PSN) adalah untuk meningkatkan pengetahuan tentang DBD. Metode yang digunakan meliputi tiga tahap yaitu pengisian kuesioner (pre-test dan post-test), pemberian edukasi (DBD, vektor, dan PSN), dan tanya jawab dengan 31 peserta didik kelas V SDN Sumur Batu 14 Pagi, Jakarta. Hasil analisis hasil pre-test dan post-test menunjukkan hasil yang signifikan, p = 0,000 artinya ada pengaruh yang signifikan setelah dilakukan penyuluhan. Kesimpulan ada peningkatan pengetahuan siswa tentang DBD dan pencegahannya.
Isolation, In Vitro Expansion, And Cryopreservation of Primary Cells Derived from Human Thyroid Carcinoma Fatimah Eliana; Sabrina Azmi; Anggi Puspa Nur Hidayati; Juhan Khalila
‎ InaJEMD - Indonesian Journal of Endocrinology Metabolic and Diabetes Vol. 2 No. 1 (2025): InaJEMD Vol. 2, No. 1
Publisher : PP PERKENI

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Abstract

Thyroid carcinoma is a malignant neoplasm arising from thyroid parenchymal cells and currently ranks as the fourth most diagnosed cancer in Indonesia. This study aimed to isolate thyroid carcinoma cells for in vitro expansion and long-term preservation as a reliable cell culture stock, including cryopreservation for future research applications. In addition, we sought to identify and characterize cells derived from papillary thyroid carcinoma (PTC) tissue to evaluate the presence of mutations with potential prognostic significance. Primary cell isolation was performed via enzymatic digestion using collagenase, enabling effective separation of tumor cells from adjacent non-malignant thyroid tissue. Cell proliferation was supported using Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 20% fetal bovine serum (FBS), selected for its high concentration of growth-promoting factors that enhance proliferation rates. For biobanking purposes, cryopreservation of the thyroid carcinoma-derived cells was conducted using a standard slow-freezing protocol. Molecular characterization was carried out through PCR amplification, gel electrophoresis, and Sanger sequencing of key oncogenic drivers, specifically the BRAF gene and five RAS gene targets: HRAS exon 2, NRAS exons 2 and 3, and KRAS exons 2 and 3. No pathogenic mutations were identified in the analyzed BRAF or RAS gene regions.
Co-Authors Anggi Puspa Nur Hidayati Fatimah Eliana Ferlianti, Rika Juhan Khalila Silpi Hamidiyah