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All Journal Jurnal Ilmiah Ilmu-Ilmu Peternakan Animal Production
Hasanah, Amanda
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Veterinary

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Optimization of Hydrolytic Enzyme Production from Tempeh Starter to Increase In Vitro Pepsin Digestibility of Black Soldier Fly Maggot Hasanah, Amanda; Rahayu, Sri; Hartoyo, Bambang; Suhartati, Fransisca Maria; Munasik, Munasik; Hindratiningrum, Novita
ANIMAL PRODUCTION Vol. 26 No. 2 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.2.278

Abstract

This research aims to evaluate the optimal level and incubation time for the production of hydrolytic enzymes from tempeh starter (TS), in order to increase the in vitro pepsin digestibility of Black Soldier Fly (BSF), Hermetia illucens, maggot flour. The materials used were commercial tempeh starter, pepsin, and 15 days of age dried BSF maggot. In this study, two experiments were conducted. In a factorial completely randomized design, the analysis of variance (ANOVA) was performed to assess the significance of treatment effects, and orthogonal polynomial contrasts were employed to identify specific linear and quadratic trends among the group means. In experiment 1, research was carried out to determine the optimal starter level and incubation time to produce hydrolytic enzymes from tempeh starter. In experiment 2, crude enzyme from the best treatment in the first stage was used to increase the pepsin digestibility of BSF maggot flour. The optimal protein content and enzyme activities of tempeh starter was 0.10-0.13% starter with an incubation time of 2.00-3.47 days. Meanwhile, the optimal pepsin digestibility of BSF maggot flour was 1.51% enzymes with a hydrolysis time of 23.87 hours. This analysis measurement of protein content and enzymatic activity of tempeh starter and in vitro digestibility of BSF flour represent the preliminary methodology used to initially select hydrolyzed BSF flour to be a source of protein in animal feed.
Optimization of Hydrolytic Enzyme Production from Tempeh Starter to Increase In Vitro Pepsin Digestibility of Black Soldier Fly Maggot Hasanah, Amanda; Rahayu, Sri; Hartoyo, Bambang; Suhartati, Fransisca Maria; Munasik, Munasik; Hindratiningrum, Novita
ANIMAL PRODUCTION Vol. 26 No. 2 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.2.278

Abstract

This research aims to evaluate the optimal level and incubation time for the production of hydrolytic enzymes from tempeh starter (TS), in order to increase the in vitro pepsin digestibility of Black Soldier Fly (BSF), Hermetia illucens, maggot flour. The materials used were commercial tempeh starter, pepsin, and 15 days of age dried BSF maggot. In this study, two experiments were conducted. In a factorial completely randomized design, the analysis of variance (ANOVA) was performed to assess the significance of treatment effects, and orthogonal polynomial contrasts were employed to identify specific linear and quadratic trends among the group means. In experiment 1, research was carried out to determine the optimal starter level and incubation time to produce hydrolytic enzymes from tempeh starter. In experiment 2, crude enzyme from the best treatment in the first stage was used to increase the pepsin digestibility of BSF maggot flour. The optimal protein content and enzyme activities of tempeh starter was 0.10-0.13% starter with an incubation time of 2.00-3.47 days. Meanwhile, the optimal pepsin digestibility of BSF maggot flour was 1.51% enzymes with a hydrolysis time of 23.87 hours. This analysis measurement of protein content and enzymatic activity of tempeh starter and in vitro digestibility of BSF flour represent the preliminary methodology used to initially select hydrolyzed BSF flour to be a source of protein in animal feed.
Penggunaan Fermeherbafit Enkapsulasi Dalam Pakan Terhadap Kadar Kolesterol Darah dan Daging Ayam Broiler Hasanah, Amanda; Hartoyo, Bambang
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 26 No 1 (2023): Mei 2023
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiiip.v26i1.24547

Abstract

Salah satu faktor pembatas konsumsi daging ayam broiler adalah kandungan kolesterolnya yang cukup tinggi. Kadar kolesterol dapat diturunkan melalui manipulasi pakan, yaitu suplementasi herbal yang difermentasi dengan probiotik (fermeherbafit) dan dienkapsulasi secara mikroskopis. Penelitian ini bertujuan untuk mengevaluasi efek penggunaan fermeherbafit enkapsulasi dalam pakan terhadap kadar kolesterol darah dan daging ayam broiler. Materi yang digunakan yaitu 80 ekor DOC ayam broiler unsex strain Multi Breeder (MB) 202 Platinum yang dipelihara sampai umur 35 hari. Percobaan dilakukan menggunakan metode eksperimental in vivo dan menggunakan Rancangan Acak Lengkap (RAL). Rancangan penelitian terdiri atas lima perlakuan dan setiap perlakuan diulang sebanyak empat kali dengan masing-masing ulangan terdiri dari empat ekor ayam. Perlakuan terdiri dari R0 = pakan basal/kontrol; R1 = penggunaan 1,5% fermeherbafit non-enkapsulasi, R2 = penggunaan 1,5% fermeherbafit enkapsulasi, R3 = penggunaan 3,0% fermeherbafit enkapsulasi, dan R4 = penggunaan 4,5% fermeherbafit enkapsulasi. Hasil analisis variansi menunjukkan penggunaan fermeherbafit enkapsulasi dalam pakan berpengaruh tidak nyata (P>0,05) terhadap kadar kolesterol darah dan berpengaruh nyata (P<0,05) terhadap kadar kolesterol daging ayam broiler. Uji lanjut Beda Nyata Jujur (BNJ) terhadap kadar kolesterol daging menunjukkan R0 berbeda nyata dengan R3 dan R4. Dapat disimpulkan penggunaan fermeherbafit enkapsulasi sampai level 4,5% dalam pakan ayam broiler menghasilkan rataan kadar kolesterol darah yang relatif sama. Penggunaan fermeherbafit non-enkapsulasi 1,5% menurunkan kadar kolesterol daging sebesar 0,79%, sedangkan penggunaan fermeherbafit enkapsulasi 4,5% menurunkan kadar kolesterol daging sebesar 1,50%.
Co-Authors Bambang Hartoyo Fransisca Maria Suhartati Munasik Munasik Novita Hindratiningrum SRI RAHAYU