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Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products Merryafinola Ifani; Efka Aris Rimbawanto; Fransisca Maria Suhartati
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2829

The study was aimed to examine effect of protecting soybean meal using mahogany leaf extract on rumen fermentation products in vitro. The material used was cow rumen fluid, basal ration consisting of concentrate and elephant grass with a ratio of 60:40%, and mahogany leaves. The research was carried out in three stages: mahogany extraction, protein protection using mahogany extract, and in vitro stages. The test was conducted in vitro based on a completely randomized design (CRD). The treatments consisted of 4 kinds of soybean meal protection with 0% tannin concentration (T0); 1.5% (T1); 3% (T2); and 4.5% (T3). Data obtained were analyzed by analysis of variance and tested using orthogonal polynomials. Results showed that addition of protected soybean meal with mahogany leaf extract had a cubical effect on partial VFA, methane gas and post-rumen dissolved protein, a quadratic effect on protozoa, and a linear effecton N-NH3, SPM, and RUDP. Giving extra mahogany leaves at a level of 1.5% produced a fermented product that was not different from the control while giving a level of 3% got the highest fermentation product. Giving mahogany leaf extract at a level of 4.5% resulted in the highest SPM, and RUDP but there was a decrease in soluble protein, which indicated the occurrence of overprotection. The addition of 3% mahogany leaf extract effectively increased rumen fermentation products, RUDP, and soluble protein without disturbing the activity of rumen bacteria.

PENGARUH DAUN TURI (Sesbania grandiflora) DAN LAMTORO (Leucaena leucocephala) DALAM RANSUM SAPI BERBASIS INDEKS SINKRONISASI PROTEIN - ENERGI TERHADAP SINTESIS PROTEIN MIKROBA RUMEN Afduha Nurus Syamsi; Fransisca Maria Suhartati; Wardhana Suryapratama
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 6 No 2 (2017): Pastura Vol. 6 No. 2 Tahun 2017
Publisher : Udayana University

An experiment was aimed to assess the use of the legume leaf as a source of protein feedstuff and levels of synchronization protein-energy (SPE) index in the diet of cattles on ammonia (N-NH3) and microbial protein synthesis (MPS). In vitro techniques was done. The research was used a completely randomized design (CRD), with factorially pattern (2x3), the first factor was the two species of legume (Sesbania leaves and Leucaena leaves) and the second factor was the three level of the SPE index (0.4, 0.5, and 0.6), there were 6 treatment combinations and each was 4 replicates. The results showed that no interaction between legume with SPE index, but each factor was significantly effect (P<0.05) on N-NH3 of rumen fluid and MPS. The research concluded that Leucaena leaf is a legume that is better than Sesbania leaf in terms of their ability toincrease MPS. SPE index is the best in producing MPS at level 0.6. Key words: Legume, synchronization of protein and energy index, ammonia, microbial protein synthesis

Fermentasi Jerami Padi Menggunakan White rot fungi dan Suplementasi Saccharomyces cerevisiae Pengaruhnya terhadap Kecernaan Nutrien Secara In Vitro Wardhana Suryapratama; Fransisca Maria Suhartati
Jurnal Agripet Vol 12, No 2 (2012): Volume 12, No. 2, Oktober 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i2.195

The influence of rice straw fermentation using white rot fungi and saccharomyces cerevisiae supplementation on in vitro nutrient digestibilityABSTRACT. An experiment to investigate the effect of rice straw fermented using white rot fungi and Saccharomyces cerevisiae supplementation on nutrient digestibility In Vitro had been implemented in two phases. The first experiments undertaken to make rice straw fermentation, using experimental methods with a Completely Randomized Design. As the treatment were White rot fungi (Phanerochaete chrysosporium) 0, 5 and 10 g/kg of rice straw (DM basis). Each treatment was repeated six times, so there are 18 experimental units. The variables measured included nutrient content of rice straw. A second experiment carried out in vitro to test the best rice straw fermentation results of the first experiment, using experimental methods, with a Completely Randomized Design. As the treatment were the supplementation of Saccharomyces cerevisiae (0, 2, 4% of the weight of fermented rice straw, DM basis). The variables measured included digestibility of dry matter, organic matter, cellulose and lignin digestibility of feed containing fermented straw. The data obtained were analyzed using analysis of variance test followed by Orthogonal Polynomials. The results can be concluded that the fermented rice straw using Phanerochaete chrysosporium 10 g/kg of rice straw is the best nutrient content. Saccharomyces cerevisiae supplementation on feed that contains fermented rice straw using Phanerochaete chrysosporium 10 g/kg rice straw is 2%.

Effect of Vitamin A and Magnesium Supplementation on Protein Consumption and Nitrogen Retention of Sheep Tsany, Muhammad Iqbal Rizqi; Suryapratama, Wardhana; Suhartati, Fransisca Maria
JURNAL ILMIAH PETERNAKAN TERPADU Vol 12, No 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p272-286

The research aimed to examine the effect of vitamin A and magnesium supplementation on protein consumption and nitrogen retention in sheep was carried out on February 29 - June 26 2023. The material used was 18 local male sheep aged 7 - 8 months with a weight of 17,12 ±1,94 kg were reared for 2 months in individual stage model cages. The basal feed given is concentrate (75%) and ammoniated rice straw (25%). The in vivo experimental method uses a Randomized Complete Block Design (RCBD) as a group, namely the initial weight of the research sheep, consisting of 6 groups that act as repeaters. Treatments tested T1 = Ammoniated rice straw (25%) + concentrate feed (75%); T2 = T1 + vitamin A (2000 IU); T3 = T2 + magnesium mineral (1 gram). The variables measured were protein consumption and nitrogen retention. The data obtained was then analyzed using analysis of variance (ANOVA) which was further tested with the Honestly Significant Difference (HSD) test. Protein consumption was significantly influenced by treatment (P<0.05) with a mean of 101.71 ± 3.85 grams/head/day (T1), 109.03 ± 8.60 grams/head/day (T2), and 115.99 ± 5.35 grams/head/day (T3). Likewise, nitrogen retention was significantly influenced by treatment (T<0.05) with an average of 9.37 ± 1.41 grams/head/day (T1), 9.81 ± 1.51 grams/head/day (T2), 10.21 ± 1.53 grams/head/day (T3). The HSD test results showed that the protein consumption and nitrogen retention of T3 sheep were significantly (P<0.05) higher than those of T1 and T2. The research conclusion was that supplementation of vitamin A and magnesium (T3) in basal feed was able to increase protein consumption by 12.31% and nitrogen retention by 8.24% in sheep when compared to the control treatment (T1).

Optimization of Hydrolytic Enzyme Production from Tempeh Starter to Increase In Vitro Pepsin Digestibility of Black Soldier Fly Maggot Hasanah, Amanda; Rahayu, Sri; Hartoyo, Bambang; Suhartati, Fransisca Maria; Munasik, Munasik; Hindratiningrum, Novita
ANIMAL PRODUCTION Vol. 26 No. 2 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.2.278

This research aims to evaluate the optimal level and incubation time for the production of hydrolytic enzymes from tempeh starter (TS), in order to increase the in vitro pepsin digestibility of Black Soldier Fly (BSF), Hermetia illucens, maggot flour. The materials used were commercial tempeh starter, pepsin, and 15 days of age dried BSF maggot. In this study, two experiments were conducted. In a factorial completely randomized design, the analysis of variance (ANOVA) was performed to assess the significance of treatment effects, and orthogonal polynomial contrasts were employed to identify specific linear and quadratic trends among the group means. In experiment 1, research was carried out to determine the optimal starter level and incubation time to produce hydrolytic enzymes from tempeh starter. In experiment 2, crude enzyme from the best treatment in the first stage was used to increase the pepsin digestibility of BSF maggot flour. The optimal protein content and enzyme activities of tempeh starter was 0.10-0.13% starter with an incubation time of 2.00-3.47 days. Meanwhile, the optimal pepsin digestibility of BSF maggot flour was 1.51% enzymes with a hydrolysis time of 23.87 hours. This analysis measurement of protein content and enzymatic activity of tempeh starter and in vitro digestibility of BSF flour represent the preliminary methodology used to initially select hydrolyzed BSF flour to be a source of protein in animal feed.

Optimization of Hydrolytic Enzyme Production from Tempeh Starter to Increase In Vitro Pepsin Digestibility of Black Soldier Fly Maggot Hasanah, Amanda; Rahayu, Sri; Hartoyo, Bambang; Suhartati, Fransisca Maria; Munasik, Munasik; Hindratiningrum, Novita
ANIMAL PRODUCTION Vol. 26 No. 2 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.2.278

This research aims to evaluate the optimal level and incubation time for the production of hydrolytic enzymes from tempeh starter (TS), in order to increase the in vitro pepsin digestibility of Black Soldier Fly (BSF), Hermetia illucens, maggot flour. The materials used were commercial tempeh starter, pepsin, and 15 days of age dried BSF maggot. In this study, two experiments were conducted. In a factorial completely randomized design, the analysis of variance (ANOVA) was performed to assess the significance of treatment effects, and orthogonal polynomial contrasts were employed to identify specific linear and quadratic trends among the group means. In experiment 1, research was carried out to determine the optimal starter level and incubation time to produce hydrolytic enzymes from tempeh starter. In experiment 2, crude enzyme from the best treatment in the first stage was used to increase the pepsin digestibility of BSF maggot flour. The optimal protein content and enzyme activities of tempeh starter was 0.10-0.13% starter with an incubation time of 2.00-3.47 days. Meanwhile, the optimal pepsin digestibility of BSF maggot flour was 1.51% enzymes with a hydrolysis time of 23.87 hours. This analysis measurement of protein content and enzymatic activity of tempeh starter and in vitro digestibility of BSF flour represent the preliminary methodology used to initially select hydrolyzed BSF flour to be a source of protein in animal feed.

PENGARUH PENGGUNAAN SILASE RUMPUT GAJAH YANG DIBERI SINGKONG TERHADAP KONSENTRASI VFA DAN N-NH3 CAIRAN RUMEN SAPI SECARA IN VITRO Fitriyanto, Rifqi; Suhartati, Fransisca Maria; Rahayu, Sri
ANGON: Journal of Animal Science and Technology Vol 3 No 3 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Background. This study was aimed to examine the effect of using elephant grass silage fed with cassava on the concentration of VFA N-NH3 which was carried out from 7 June to 7 July at the Laboratory of Animal Nutrition and Food Science, Faculty of Animal Husbandry, Universitas Jenderal Soedirman Purwokerto. Materials and methods The materials used in this study were rumen fluid from three beef cattles taken from the slaughterhouse of Bantarwuni Village, Kembaran District, Banyumas Regency immediately after the cattle were slaughtered, silage of elephant grass, cassava, and a set of in vitro tools. The study was carried out using in vitro methods and using a randomized design (CRD) with 4 complete treatments. Each treatment was repeated 5 times. The treatments tested were R1 = 100% DM of elephant grass silage; R2 = 95% DM of elephant grass silage + 5% DM of cassava; R3= 90% DM of elephant grass silage + 10% DM of cassava; R4 = 85% DM of elephant grass silage + 15% DM of cassava. The variables measured were the concentration of VFA and N-NH3. The data obtained were analyzed for variance and if the treatment had an effect on the measured variables, it was continued with the Honestly Significant Difference (HSD) test. Results. The average concentration of VFA is R1 : 122 mM, R2 : 126 mM, R3 : 128 mM, R4 : 130 mM, while the average concentration of N-NH3 is R1 : 2.4 mM, R2 : 3.4 mM, R3 : 4 mM, R4 : 3 mM. The results of the analysis of variance shows that the use of elephant grass silage fed with cassava had no significant effect (P>0.05) on the concentration of VFA but has a very significant effect (P<0.01) on the concentration of N-NH3. The highest VFA concentration is achieved by rumen fluid which obtained 85% silage of elephant grass and 15% cassava, although statistically there is no difference. Conclusion. The average concentration of N-NH3 is still low, but the highest concentration is achieved by rumen fluid added with 90% elephant grass silage and 10% cassava.

PRODUK FERMENTASI RUMEN SAPI POTONG SECARA IN VITRO YANG DIBERI PAKAN SILASE DAUN NANAS SEBAGAI PENGGANTI RUMPUT GAJAH Saputro, Alif Rizki Tixko; Suhartati, Fransisca Maria; Rimbawanto, Efka Aris
ANGON: Journal of Animal Science and Technology Vol 4 No 1 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Background. This study aims to examine the effect of using pineapple leaf waste silage as a substitute for elephant grass based on levels of Nitrogen-ammonia (N-NH3) and Volatile Fatty Acid (VFA) concentration in total rumen fluid of beef cattle in vitro. The research was conducted from August 25 to September 20, 2022 at the Laboratory of Nutrition and Animal Feed Science, Faculty of Animal Science, Jenderal Sudirman University, Purwokerto. Materials and methods. The study using an in vitro method with a completely randomized design, 5 treatments and each treatment was repeated 4 times so that there were 20 research units. The treatments used consisted of P0 (60% concentrate + 40% elephant grass), P1 (60% concentrate + 30% elephant grass + 10% pineapple leaf silage), P2 (60% concentrate + 20% elephant grass + 20% pineapple leaf silage), P3 (60% concentrate + 10% elephant grass + 30% pineapple leaf silage), and P4 (60% concentrate and 40% pineapple leaf silage). Results. The results showed that the treatment had no significant effect (P>0.05) on the levels of N-NH3 and the concentration of VFA. Conclusion. It was concluded that the use of pineapple leaf silage at a balance of 40% elephant grass and 60% concentrate could replace elephant grass in beef cattle feed up to 100% based on in vitro measurements of N-NH3 levels and total VFA concentrations.

konsumsi KONSUMSI DAN KECERNAAN PROTEIN KASAR PAKAN SAPI BALI JANTAN YANG DIBERI COMPLETE RUMEN MODIFIER Syafiq, Muhammad; Suhartati, Fransisca Maria; Yuwono, Pambudi
ANGON: Journal of Animal Science and Technology Vol 6 No 3 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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ABSTRACT Research aimed to examine the effect of giving Complete Rumen Modifier on the intake and digestibility of Crude Protein (CP) feed of Bali bulls. The material used is 3 male Balinese cows, the feed given consists of concentrate 2% of the cow's body weight and voluntary feed intake hay. The concentrate is composed of onggok 38,58%, rice bran 14,29%, coconut cake 13,14%, palm cake 10%, pollard 9,43%, molasses 4,86%, coffee husk 4,8%, dolomite 1,43%, aminosine 1,2%, salt 0,86%, urea 0,86%, zeolite 0,57%, and minerals 0,28%. The composition of CRM consists of noni leaf flour 30%, cassava vine leaf meal 30%, dry tea pulp 30%, Saccharomyches cerevisiae 3%, methionin 2%, urea 1% and sulfur 4%. The research used the experimental method of Latin Square Design, there were three treatments tested, namely P0 (concentrate 2% body weight cow and straw voluntary feed intake), P1 (P0 + CRM 0.5% from Dry Matter concentrate), and P2 (P0 + CRM 1% from concentrate). The variables measured are intake and Digestibility of crude protein, the data obtained were tested using analysis of variance and differences in treatment with the Orthogonal Polynomial test. The results of the analysis of variance showed that the treated feed given CRM had no significant effect (P>0.05) on PK digestibility. Meanwhile, PK consumption had a significant effect (P<0.05) on feed treated with P1 and P2 compared to P0. The conclusion obtained from this study is that the provision of CRM can increase consumption of PK while the digestibility of PK decreases when given CRM.

the effect PENGARUH PENGGANTIAN KONSENTRAT MENGGUNAKAN AMPAS TEMPE DAN SUPLEMENTASI COMPLETE RUMEN MODIFIER (CRM) TERHADAP KONSUMSI DAN KECERNAAN PROTEIN KASAR PAKAN DOMBA Hutasoit, Irma Uli; Suhartati, Fransisca Maria; Suryapratama, Wardhana
ANGON: Journal of Animal Science and Technology Vol 6 No 3 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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The study aims to examine the effect of concentrate replacement using tempeh dregs and Complete Rumen Modifier (CRM) supplementation on the consumption and digestibility of crude protein in sheep feed. The study used 18 local male sheep aged 7-8 months with an average weight of 20±2.34 kg. This study used a Randomized Group (RAK) design and used a type of experimental research, as a group as well as a repeat of the initial body weight of sheep after the adaptation period as many as 6 groups and 3 treatments, including P1: Ammoniated rice straw ad libitum + concentrate 2.5% DM of body weight (control), P2: Ammoniated rice straw ad libitum + tempeh dregs 2.5% DM of body weight, and P3: Ammoniated rice straw ad libitum + tempeh dregs 2.5% DM of body weight + 1% CRM. The variables observed in the study were consumption and digestibility of crude protein. Based on the results of the analysis of treatment variance, the effect was very real (P<0.01) on crude protein consumption with an average consumption of P1, P2, P3 treatments, respectively 10.26±0.05; 14.35±0.78; 14.02±0.88 (g/head/d) and a significant effect (P<0.005) on digestibility with crude protein digestibility rate of P1, P2, P3 treatment respectively at 59.44±3.65%; 67.85±3.44%; 63.56±4.83%. Based on orthogonal contrast tests, tempeh dregs can replace concentrate feed entirely and even increase crude protein consumption and digestibility by 3.90% and 3.93% respectively without 1% CRM supplementation. Keywords: CRM, tempe dregs, lamb, crude protein, consumption, digestibility.

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