Panasea Anggy, Fransiska
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Molecular Detection of Jembrana Disease in Balinese Cattle (Bos sondaicus) Samples at the Lampung Veterinary Center Kurniasih, Vidyanti; Rustiati, Elly Lestari; Srihanto, Eko Agus; Firwantoni, Firwantoni; Heni, Ahyul; Panasea Anggy, Fransiska; Febriyani, Dwi Ayu
Jurnal Agrosci Vol 2 No 3 (2025): Vol 2 No 3 January 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i3.578

Abstract

Jembrana Disease is an infectious disease in Balinese cattle, which is characterized by fever and inflammation of the oral mucous membranes (stomatitis, enlarged lymph nodes of the preskapularis, prefemolaric, and parotid, sometimes accompanied by blood sweating. This disease reaches a mortality rate of no more than 20% caused by the Jembrana Disease Virus (JDV). According to data from the Directorate of Animal Health, Jembraa Disease cases continue to increase from year to year, with as many as 398 cases in 2016 and increased to 4,797 cases in 2018. The presence of the Jembrana virus can be detected through tests. One of them is by identifying and isolating the pathogen. Technological breakthroughs in DNA-based testing systems are becoming an available alternative. Viruses can be detected through commonly used molecular analysis such as Polymerase Chain reaction (PCR). The Jembrana Disease test was carried out at the Biotechnology Laboratory of the Lampung Veterinary Center using PCR and then visualized by agarose gel electrophoresis. The test results of 53 samples consisting of 50 types of buffycoat samples and 3 types of organ samples showed that 1 type of organ sample was detected with JDV
Molecular Detection of Lumpy Skin Disease in Cattle Samples (Bos taurus) at Lampung Disease Investigation Center Yunior, Elfita Nova; Rustiati, Elly Lestari; Srihanto, Eko Agus; Firwantoni, Firwantoni; Heni, Ahyul; Panasea Anggy, Fransiska; Febriyani, Dwi Ayu
Jurnal Agrosci Vol 2 No 4 (2025): Vol 2 No 4 March 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i4.640

Abstract

Lumpy Skin Disaese, LSD, was first discovered in Indonesia in Riau Province in February 2022. Although LSD is not a zoonotic disease, its infection can affect the production and reproductive performance of cattle, resulting in various impacts including reduced milk production, weight loss, miscarriages, and infertility as well as significant economic losses. It is a vector-borne smallpox disease of cattle and buffalo. Characterized by the appearance of pustules on the skin. Identification of LSDV can be carried out by three methods, namely Polymerase Chain Reaction (PCR), virus isolation, and electron microscopy. PCR is the cheapest and fastest method to detect LSDV. LSD disease testing at the Biotechnology Laboratory, Lampung Disease Investigation Center using the RT- PCR method. No LSD was found over 13 blood samples and 2 nasal swab samples from January 2024 sampling showed that all of them were negative to LSD.