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All Journal Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biosfer: Jurnal Tadris Biologi Jurnal Biologi Tropis Jurnal Biogenerasi BIOVALENTIA: Biological Research Journal Journal Of Agriculture Sciences (Agrosci)
Eko Agus Srihanto, Eko Agus
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION Rustiati, Elly Lestari; Priyambodo, Priyambodo; Yulianti, Yanti; Srihanto, Eko Agus; Pratiwi, Dian Neli; Virnarenata, Elsa; Novianasari, Tika; Krismuniarti, Elisabeth Devi; Saswiyanti, Enny
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.173 KB) | DOI: 10.24233/BIOV.6.1.2020.173

Abstract

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.
IDENTIFICATION AND CHARACTERISATION OF COI GENE IN FEMALE SUMATRAN ELEPHANT (Elephas maximus sumatranus) IN ELEPHANT TRAINING CENTRE, WAY KAMBAS NATIONAL PARK Virnarenata, Elsa; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Pratiwi, Dian Neli
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (716.49 KB) | DOI: 10.24233/biov.7.1.2021.175

Abstract

Sumatran elephant is a subspecies of endemic Asian elephants on the island of Sumatra and is included in the Red List of the International Union for Conservation of Nature (IUCN) with critically endangered status. The building of the Elephant Training Centre (ETC) in Way Kambas National Park (WKNP) is one of the conservation efforts of Sumatran elephants. Small and closed population size lead to an increased risk of inbreeding that triggers reduction in genetic variation and viability and increases the risk of extinction. The phylogenetic pattern of Sumatran elephants in Indonesia has shown a low population genetic diversity. Genetic diversity information is indispensable to support the direction of decision making in Sumatran elephant conservation policy. The DNA isolation of Sumatran elephants in ETC, WKNP has performed as a first step to trace its genetic variation. The advanced step of DNA isolation is the use of cytochrome oxidase subunit I (COI) gene for identification of genetic characteristics in Sumatran elephants. The COI gene is one of the genes on the mitochondrial genome and in molecular studies it is used as a genetic marker to study genetic characteristics between species and individuals. Identification and characterisation are done by sequencing process and data analysis in the form of electroforegram using Molecular Evolution Genetics Analysis (MEGA) software version 6.0. to see the genetic diversity of the female Sumatran elephant population in ETC, WKNP. Based on the results of the analysis it is indicated that the genetic distance of 24 individual female Sumatran elephant from PLG, TNWK is 0.000 with a homology value of 100%, strengthened by the construction of phylogenetic tree. The absence of genetic distance indicates a close genetic relationship, so it can be concluded all individual female Sumatran elephants in the PLG, TNWK is derived from one population group.
Molecular Detection of Jembrana Disease in Balinese Cattle (Bos sondaicus) Samples at the Lampung Veterinary Center Kurniasih, Vidyanti; Rustiati, Elly Lestari; Srihanto, Eko Agus; Firwantoni, Firwantoni; Heni, Ahyul; Panasea Anggy, Fransiska; Febriyani, Dwi Ayu
Jurnal Agrosci Vol 2 No 3 (2025): Vol 2 No 3 January 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i3.578

Abstract

Jembrana Disease is an infectious disease in Balinese cattle, which is characterized by fever and inflammation of the oral mucous membranes (stomatitis, enlarged lymph nodes of the preskapularis, prefemolaric, and parotid, sometimes accompanied by blood sweating. This disease reaches a mortality rate of no more than 20% caused by the Jembrana Disease Virus (JDV). According to data from the Directorate of Animal Health, Jembraa Disease cases continue to increase from year to year, with as many as 398 cases in 2016 and increased to 4,797 cases in 2018. The presence of the Jembrana virus can be detected through tests. One of them is by identifying and isolating the pathogen. Technological breakthroughs in DNA-based testing systems are becoming an available alternative. Viruses can be detected through commonly used molecular analysis such as Polymerase Chain reaction (PCR). The Jembrana Disease test was carried out at the Biotechnology Laboratory of the Lampung Veterinary Center using PCR and then visualized by agarose gel electrophoresis. The test results of 53 samples consisting of 50 types of buffycoat samples and 3 types of organ samples showed that 1 type of organ sample was detected with JDV
Supporting Environmental Resilience through Biological Approach: Initial Step for Building Robusta Coffee Germplasm Database Rustiati, Elly Lestari; Priyambodo, Priyambodo; Pratiwi, Dian Neli; Srihanto, Eko Agus; Lestari, Septi Wahyu; Sandra, Shifa; Winarno, Winarno; Thesalonika, Natasya
Bioma : Berkala Ilmiah Biologi Vol. 26 No 2, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.65548

Abstract

In response to environmental challenges and the critical imperative for sustainable progress, rural regions including agriculture practices are important in ecological robustness. It can be achieved through a synergistic relationship between rural development sustainability and the biological sciences. Concurrently, research in stewardship approaches are underway to amass data on robusta coffee, Coffea canephora, species. In collaboration with Lampung Disease Investigation Centre and local coffee farmers in Pesawaran, Lampung, and under DIPA BLU Universitas Lampung year 2024, initial ongoing work on coffee germ plasm data is done. Robusta coffee leaves were sampled from 2 blocks of traditional coffee plantations, Wiyono and Bogorejo, Pesawaran, Lampung.  Of 18 robusta leaves’ samples DNA extraction, electrophoresis showed 14 samples with good results. The optimum temperature for amplification is 62 °C and 63 °C.  The results will be sequenced for its species confirmation.  This project is expected to contribute bottom up towards inclusive and sustainable rural futures at the local level.
Molecular Detection of Lumpy Skin Disease in Cattle Samples (Bos taurus) at Lampung Disease Investigation Center Yunior, Elfita Nova; Rustiati, Elly Lestari; Srihanto, Eko Agus; Firwantoni, Firwantoni; Heni, Ahyul; Panasea Anggy, Fransiska; Febriyani, Dwi Ayu
Jurnal Agrosci Vol 2 No 4 (2025): Vol 2 No 4 March 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i4.640

Abstract

Lumpy Skin Disaese, LSD, was first discovered in Indonesia in Riau Province in February 2022. Although LSD is not a zoonotic disease, its infection can affect the production and reproductive performance of cattle, resulting in various impacts including reduced milk production, weight loss, miscarriages, and infertility as well as significant economic losses. It is a vector-borne smallpox disease of cattle and buffalo. Characterized by the appearance of pustules on the skin. Identification of LSDV can be carried out by three methods, namely Polymerase Chain Reaction (PCR), virus isolation, and electron microscopy. PCR is the cheapest and fastest method to detect LSDV. LSD disease testing at the Biotechnology Laboratory, Lampung Disease Investigation Center using the RT- PCR method. No LSD was found over 13 blood samples and 2 nasal swab samples from January 2024 sampling showed that all of them were negative to LSD.
Sex Determination on Racing Pigeons (Columba livia) Molecularly Using Blood Samples Azzahra, Laila Salwa; Srihanto, Eko Agus; Rustiati, Elly Lestari; Master, Jani; Firwantoni, Firwantoni; Febriyani, Dwi Ayu; Pratiwi, Dian Neli
Jurnal Agrosci Vol 2 No 6 (2025): Vol 2 No 6 July 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i6.785

Abstract

Background. Pigeons (Columba livia) belong to the Columbidae. Pigeons possess diverse ecological, economic, and aesthetic values, as they are monomorphic, exhibiting similar characteristics between male and female individuals, especially at a young age. Aims. In collaboration with the Lampung Disease Investigation Center, a study has been done to confirm the sex determination of pigeons at a young age by the PCR technique. Blood samples were taken in two locations, East Lampung and Kota Metro. Molecular análisis was carried out at the Biotechnology Laboratory, Lampung Disease Investigation Center. Methods. The technique includes DNA extraction, DNA amplification, electrophoresis and visualization. Result. It poses a significant challenge for pigeon breeders in sex determination. Blood is a source of genetic material that can be used to determine the sex of birds. The Chromo-Helicase-DNA-binding Protein (CHD) gene is one of the genes used as a marker to molecularly differentiate the sex of birds. The sex chromosomes in female individuals have heterozygous chromosomes (ZW) and male individuals have homozygous sex chromosomes (ZZ). Conclusion. Of eleven pigeon blood samples, there were seven female and four male individuals, and there was one mistake in the gender prediction by the pigeon owner
Sex Determination in Racing Pigeons (Columba livia) Molecularly Using Young Feather Samples Adelia, Ersa Imelda; Srihanto, Eko Agus; Rustiati, Elly Lestari; Master, Jani; Firwantoni, Firwantoni; Anggy, Fransiska Panasea; Neli Pratiwi, Dian
Jurnal Agrosci Vol 2 No 6 (2025): Vol 2 No 6 July 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i6.807

Abstract

The racing pigeon (Columba livia) has an aesthetic value and is in high demand. Pigeon is monomorphic, difficult to distinguish between male and female individuals, especially at the age of offspring. Knowing the sex of birds from an early age is necessary to prepare male bird individual as racing bird. Accurate determination of the sex of racing pigeons from an early age was carried out to have scientific confirmation of the molecular method of determining the sex in racing pigeon. Under Lampung Disease Investigation Center program, molecular methods of PCR techniques using primers 2550F and 2718R were applied to determine the sex of bird based on the presence of Z and W chromosomes from young feathers of racing pigeons. Sampling young feathers was done in individual breeders in Kota Metro and East Lampung. Molecular analysis was carried out at the Biotechnology Laboratory of the Lampung Disease Investigation Center, consisted of extraction, amplification, electrophoresis, and data analysis. Fifteen young feather samples showed five male individuals with electrophoresis visualization formed one band measuring 600 bp and ten female individuals showed two bands measuring 600 bp and 400 bp.
AMPLIFIKASI GEN COI PADA SAMPEL LEBAH TANPA SENGAT DI LAMPUNG TENGAH: ANALISIS SECARA KUALITATIF Andriyani, Yuliana; Priyambodo, Priyambodo; Rustiaati, Elly Lestari; Pratami, Gina Dania; Ashari, Minanti Mayda; Srihanto, Eko Agus; Pratiwi, Dian Neli; Adelia, Ersa Imelda; Azzahra, Laila Salwa; Lestari, Septi Wahyu; Sandra, Shifa
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5539

Abstract

Indonesia memiliki keanekaragaman flora dan fauna yang tinggi dengan iklim tropis yang dimilikinya. Lebah tanpa sengat berhabitat di daerah tropis dan memiliki keanekaragaman yang tinggi dan menghasilkan produk madu dan propolis serta telah banyak dibudidayakan. Lampung Tengah sebagai salah satu daerah yang memiliki lokasi budidaya lebah tanpa sengat, dipilih sebagai lokasi penelitian. Penelitian telah dilakukan dengan analisis molekuler menggunakan penanda molekuler gen COI. Penelitian ini bertujuan untuk mengetahui kualitas hasil amplifikasi gen COI pada empat individu lebah tanpa sengat yang dikoleksi di Lampung Tengah. Analisis molekuler telah dilakukan melalui proses ekstraksi, amplifikasi, elektroforesis, dan visualisasi DNA. Kualitas DNA diketahui melalui pita DNA yang terbentuk pada proses elektroforesis gel agarosa 1% dan divisualisasikan di bawah sinar ultraviolet. Hasil amplifikasi gen COI pada empat individu lebah tanpa sengat memiliki kualitas pita DNA yang baik dengan panjang base pair berkisar antara 600 – 750bp.
ANALISIS HASIL AMPLIFIKASI SAMPEL INDIVIDU LEBAH TANPA SENGAT BERDASARKAN GEN COI DI LAMPUNG TIMUR Ashari, Minanti Mayda; Priyambodo, Priyambodo; Rustiati, Elly Lestari; Pratami, Gina Dania; Andriyani, Yuliana; Srihanto, Eko Agus; Pratiwi, Dian Neli; Azzahra, Laila Salwa; Adelia, Ersa Imelda; Sandra, Shifa; Lestari, Septi Wahyu; Ramadan, Viki
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5540

Abstract

Lebah tanpa sengat masuk ke dalam suku meliponidae dan merupakan lebah yang tidak menggunakan daya sengat sebagai alat pertahanan diri melainkan gigitan dan kerumunan apabila berada dalam bahaya. Indonesia memiliki keragaman jenis lebah tanpa sengat dan telah ditemukan sekitar 52 jenis yang tersebar di Pulau Sumatera, Kalimantan, Jawa, Timor, Sulawesi, Ambon, Maluku dan Irian Jaya. Dari beragam jenis lebah tanpa sengat yang ditemukan di Indonesia menunjukkan bahwa adanya keragaman genetik yang tinggi. Penelitian ini bertujuan untuk melakukan analisis hasil amplifikasi secara kualitatif dari DNA lebah tanpa sengat berdasarkan gen COI. Penelitian dilaksanakan pada rentang bulan Oktober 2024 - Januari 2025. Tahapan dalam penelitian ini meliputi pengambilan sampel, ekstraksi dan amplifikasi DNA. Hasil ekstraksi dan amplifikasi diuji dengan mengggunakan elektroforesis. Berdasarkan analisis kualitatif menggunakan gel agarose 1%, keempat sampel lebah tanpa sengat berhasil diekstraksi DNAnya. Ukuran panjang DNA dari masing-masing sampel lebah tanpa sengat diketahui berkisar antara 600-700bp dari hasil uji kualitatif setelah amplifikasi DNA.
UJI KEBERHASILAN AMPLIFIKASI GEN N-METHYLTRANSFERASE PADA KOPI ROBUSTA DI PERKEBUNAN KOPI RAKYAT LEMBAH GUNUNG BETUNG, BOGOREJO, LAMPUNG Sandra, Shifa; Rustiati, Elly Lestari; Priyambodo; Wahyuningsih, Sri; Srihanto, Eko Agus; Pratiwi, Dian Neli; Febriansyah, Muhammad; Lestari, Septi Wahyu; Thesalonika, Natasya; Winarno; Ashari, Minanti Mayda; Andriyani, Yuliana; Alfandi, Aril; Maharani, Annisa Lidya; Suhada, Suhada; Permatasari, Nindy
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5542

Abstract

Indonesia memiliki dua jenis kopi yang umum dibudidayakan, yaitu kopi robusta dan kopi arabika. Di Lampung, kopi robusta menjadi jenis kopi yang banyak ditanam. Masyarakat Lampung mayoritas melakukan budidaya di perkebunan tradisional. Keanekaragaman hayati pada kopi robusta secara genetik menggunakan analisis molekuler di perkebunan kopi rakyat masih belum banyak dilakukan. Tujuan dari penelitian ini untuk uji keberhasilan amplifikasi gen N-Methyltransferase pada kopi robusta di Perkebunan Kopi Rakyat Lembah Gunung Betung, Bogorejo, Lampung. Tahap penelitian ini meliputi ekstraksi DNA untuk mendapatkan DNA murni, mengetahui ukuran amplikon DNA melalui amplifikasi DNA menggunakan commercial kit, dan elektroforesis 1% gel agarosa. Hasil amplifikasi DNA pada lima sampel daun kopi robusta memperoleh pita DNA dengan ukuran berkisar antara 630-650 bp, tidak terdapat smear, namun terdapat variasi ketebalan pita DNA. Kata Kunci: N-methyltransferase, kopi robusta, Polymerase Chain Reaction
Co-Authors Adelia, Ersa Imelda Afandi, Aril Alfandi, Aril Anggy, Fransiska Panasea Ashari, Minanti Mayda Azzahra, Laila Salwa Elly Lestari Rustiati Fahrezi, Aditya Febriansyah, Muhammad Febriyani, Dwi Ayu Firwantoni, Firwantoni Gina Dania Pratami, Gina Dania Heni, Ahyul Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Neli Pratiwi, Dian Novianasari, Tika Panasea Anggy, Fransiska Permatasari, Nindy Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Ramadan, Viki Ramadhan, Viki Rustiaati, Elly Lestari Sa'uddah, Lousanja D. Sandra, Shifa Saswiyanti, Enny Sidik, Mahfud Sri Wahyuningsih Suhada Suhada, Suhada Susanto, Alvin Wiwiet Thesalonika, Natasya Virnarenata, Elsa WINARNO Winarno Winarno Yani, Ani Andri Yanti Yulianti Yuliana Andriyani Yunior, Elfita Nova